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Citations for Thermo Fisher :
1851 - 1900 of 6549 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... consisting of DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml 1% penicillin/ streptomycin (pen/strep; all Thermo Fisher Scientific, Waltham, MA, USA) + 20 ng/ml BDNF + 20 ng/ml GDNF + 20 ng/ml NGFb (all Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... collected as single cell suspensions using cell strainer and resuspended in sphere formation medium which was composed of 1:1 DMEM and F-12 nutrient mix supplemented with B-27 and N-2 (Thermo Fisher Scientific, Waltham, MA, USA). 1,000 cells were plated in each well of ultra-low attachment 6-well plates (Corning ...
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bioRxiv - Genetics 2022Quote: ... Over the course of the following 4 days, the colonies were gradually transitioned to neural induction medium (NIM) (consisting of DMEM/F12, 1% N-2 supplement (Fisher Scientific, Cat #: 17-502-048), MEM non-essential amino acids (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Mtb-SC and Mtb-AG-infected rabbit lungs (n=3 per group per time point) was isolated using TRIzol reagent (Life Technologies, Grand Island, NY, USA) as described earlier (5 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA from each sample was used to generate amplified and biotinylated sense-strand cDNA from the entire expressed genome according to the Sensation Plus FFPE Amplification and WT Labeling Kit (P/N 703089, Rev.4 Thermo Fisher Scientific Inc., Life Technologies). GeneChip ST Arrays (GeneChip Human Gene 2.1 ST Array Plate ...
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bioRxiv - Molecular Biology 2021Quote: ... and hNAA40 vectors served as templates to generate attB-flanked hNAA40 L(dTIS>CTC) and hNAA40S PCR products suitable for use in a Gateway® BP recombination reaction with a donor vector (pDONR221, Invitrogen, cat n°12536-017) thereby creating an entry clone ...
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bioRxiv - Biophysics 2022Quote: ... supernatant discarded and the cell pellet dissolved by suspension in 300 µL of N-PER™ Neuronal Protein Extraction Reagent (Thermo Scientific catalog number: 87792) lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... After staining of the transferred proteins with amido black the cleavage product was cut out for sequencing via N-terminal Edman degradation performed on a Procise Protein Sequencer 494C (Applied Biosystems, Foster City, United States).
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from hiPSC-derived cortical neurons at DIV8 (Q5, Q6, n=3 each group) using the miRVana miRNA isolation kit (#AM1560, Ambion, Thermo Fisher Scientific, Waltham, MA, USA) in accordance to their manuals ...
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bioRxiv - Bioengineering 2024Quote: A separate set of ATA samples (n=4 per group) were immersed in optimal cutting temperature compound (OCT; 4585, Thermo Fisher Scientific; Waltham, MA, USA) immediately after excision and frozen at 80◦C for fluorescent immunostaining ...
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bioRxiv - Cell Biology 2024Quote: ... Silencer® Select N°1 negative control siRNA or predesigned Silencer® Select siRNAs for ZNF827 (s45694, s45695, s45696) (Thermo Fisher Scientific, Waltham, MA, USA) were used for the transfection process ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR of the cDNA was performed using a primer set for the N gene of RSV (45) and ACTB (Applied Biosystems, TaqMan Assay ID: Hs99999903_m1) as a housekeeping gene ...
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bioRxiv - Cell Biology 2023Quote: ... Expression constructs were made by LR reaction using the following destination vectors: pDEST15 (for N-terminal GST-fusion in E. coli, cat#11802014, Thermo Fisher Scientific, Waltham, MA, USA) and pcDNA-DEST53 (for N-terminal GFP fusion in mammalian cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Surface CRT was detected using anti- CRT(N) or anti-CRT(Cmut) antibody (1:200 dilution) followed by staining with Alexa Fluor™ 647 conjugated secondary antibody (Invitrogen A21245, 1:2000 dilution). Total CRT in healthy donor platelets was stained with anti-CRT(N ...
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bioRxiv - Neuroscience 2023Quote: ... KSR medium was replaced by N2 medium (N2; DMEM/F12 GlutaMAX + 1X B-27 Plus Supplement + 1X N-2 Supplement + 100 U/ml pen/strep [all: Thermo Fisher Scientific, Waltham, MA, USA]), supplemented with 2i + 3i in 25% increments every two days until day 10 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein band corresponding to Fragment 2 was cut out and subjected to N-terminal sequencing analysis (Matsudaira, 1987) on an automated protein sequencer (#Procise 491 cLc; Applied Biosystems, Foster City, CA, USA) using the standard pulsed-liquid program for PVDF-blotted proteins.
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bioRxiv - Plant Biology 2024Quote: Three replicates of 50 after-ripened mature seeds were sterilized as above and subsequently imbibed on moist nitrocellulose filter papers (Whatman n°1; Thermo Fisher Scientific; Waltham, MA, USA) placed on 55 mm Petri dishes containing solid MS/2 with or without 1 μM 2-cis ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells expressing TMPRSS2 proteins or its domains in 6-well plates were washed with PBS and incubated with EZ-Link™ Sulfo-N-Hydroxy Succinimide– SS-Biotin (0.25 mg/ml, 1 ml/well) (Thermo Fisher Scientific; Cat No: A44390) label for 20 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: PCL nanofibrous sheets were cut into discs (16 mm diameter, n=24) using an Acuderm Acu-Punch Biopsy Punch® (Acuderm Inc., Fisher Scientific Ltd., Loughborough, UK) and inserted into a 24-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... KSR medium was replaced in 25%-steps with N2 medium (N2; DMEM/F12 GlutaMAX, 1X B-27 Plus Supplement, 1X N-2 Supplement, 100 U/ml penicillin/streptomycin [all: Thermo Fisher Scientific, Waltham, MA, USA]) every other day ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... The test was carried out at the optimized temperature determined as described above with four positive controls (1 pg pUC57-N DNA) and four negative controls (ddH2O) in a StepOne™ System (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Cancer Biology 2019Quote: Treated cell-pellets were lysed and digested using the commercially available Pierce™ Mass Spec Sample Prep Kit for Cultured Cells (Thermo Fisher Scientific, P/N 84840) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA) (1 ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from BALB/cByJ x C57BL/6J F1 hybrid dissected spleens (n = 4) preserved in RNA later (Thermofisher, Cat. No. AM7020; Waltham, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2022Quote: Untargeted lipidomics profiling (n = 3) was performed via high-resolution liquid chromatography with tandem mass spectrometry (Thermo Scientific QExactive Plus/HF Orbitrap HR-LC-MS/MS) at the Mass Spectrometry Core at Beth Israel Deaconess Medical Center in Boston ...
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bioRxiv - Systems Biology 2020Quote: ... Figure S5 shows the knockdown efficiency of siRNA transfections in each cell line with GAPDH siRNA compared to no siRNA (just N-TER transfection reagent) and negative control siRNA by Western blot (GAPDH antibody: Cell Signaling Technology, Cat#2118; Tubulin antibody: Thermo Fisher Scientific, Cat#62204). Experiments were performed 24hr after transfection.
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bioRxiv - Pathology 2023Quote: ... Xcc 8004 and Xe 85-10 strains were synthesized as gene fragments with appropriate BpiI sites (GeneArt gene synthesis by Thermo Scientific; codon-optimized for N. benthamiana) and subcloned as level 0 CDS1ns modules (in pAGM1287 ...
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bioRxiv - Neuroscience 2023Quote: ... pellets were reconstituted in 50[µl of 0.1[N NaOH and kept for protein quantification using a BCA protein assay kit (Thermo Scientific Pierce BCA Protein Assay Kit, PI23227). Dialysate concentrations are expressed as ng/ml and tissular concentrations as ng/mg of total proteins.
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bioRxiv - Physiology 2023Quote: ... The tissue or whole body samples were homogenized in 1 N HCl (100 µl per 10 tissues or whole body) manually using plastic pestles (Thermo Fisher Scientific Inc., Waltham, MA, USA). To enhance homogenization and solubilization ...
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bioRxiv - Cell Biology 2020Quote: ... cells were co-stained with 16.7 nM tetramethylrhodamine methyl ester (TMRM; Invitrogen) and 100 nM Mitotracker Green (MTG ...
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bioRxiv - Microbiology 2020Quote: ... PobA activity was inhibited with methyl 4-hydroxy-3-iodobenzoate (Fisher Scientific) at a saturating concentration (0.48 mM) ...
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bioRxiv - Microbiology 2021Quote: ... Cysteine alkylation was induced by addition of methyl methanethiolsulfonate (MMTS, Thermo Scientific) in 100% isopropanol to obtain a final concentration of 10 mM followed by a 10 min incubation in a thermoshaker at 37°C ...
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bioRxiv - Immunology 2021Quote: For measurement of mitochondrial membrane potential TMRM (Tetramethylrhodamine, Methyl Ester, Perchlorate; ThermoFisher) was added to the cells at 30 nM final concentration ...
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bioRxiv - Molecular Biology 2023Quote: To assess paraquat (Methyl viologen 98%, Thermo Fisher Scientific Cat. No.: 227320050) toxicity ...
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bioRxiv - Developmental Biology 2022Quote: ... using donor embryos labeled with tetramethyl rhodamine methyl ester (TMRM, Fisher Scientific). To produce contemporaneous stage-matched embryos for PMC transplants ...
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bioRxiv - Microbiology 2023Quote: ... proteins were eluted with a 1M α-Methyl-D-mannopyranoside (Acros Organics) pH8 solution.
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM MS(PEG)4 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) at 30 °C for 45 minutes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 10.6 mM Nα-tosyl-L-arginine methyl ester HCl (Fisher Scientific, T03301G), 10.8 mM tosyl-lysine-chloromethylketone HCl (Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... interleukin 6 (IL-6; Invitrogen, Carlsbad, CA), plasminogen activator inhibitor 1 (PAI-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Alkaline phosphatase staining was performed using the one-step nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP) solution (Thermofisher).
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bioRxiv - Microbiology 2023Quote: ... was used for induction of gene expression and X-gal (X-Gal 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside; Thermofisher) TSA plates were used for bacterial assessment ...
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bioRxiv - Molecular Biology 2021Quote: The human HCT 116 colon cancer cell line was kindly provided to us by the Johns Hopkins Sidney Kimmel Comprehensive Cancer Center (Baltimore, USA) and cultivated in McCoy’s medium (Gibco™, Thermo Fisher Scientific Inc., cat n°22330-021). K-562 (ATCC ...
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bioRxiv - Microbiology 2023Quote: ... The template for generating homology-directed repair DNA for gene tagging was constructed by inserting a P2A viral skip peptide in frame with a downstream blasticidin-S deaminase (BSD) or puromycin N-acetyl-transferase (puro) and TOPO cloned into a pCR4 backbone (Thermo Fisher, Waltham, MA, United States of America) 51 ...
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bioRxiv - Neuroscience 2023Quote: ... The original INNOTEST kit plate was replaced with a 96-well MicroWell™ MaxiSorp™ flat bottom plate coated with V5 antibody (Invitrogen P/N-0705; 1:10000) in carbonate buffer (NaHCO3 0.1 mM ...
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bioRxiv - Cell Biology 2021Quote: ... total protein extracts were prepared from hearts homogenized and solubilized in radioimmunoprecipitation assay (RIPA) buffer supplemented with 2.4 mg/ml nicotinamide and a combined protease and phosphatase inhibitor cocktail (Thermo Fisher). Cardiac mitochondrial protein extracts were prepared by solubilizing heart mitochondria in the same RIPA buffer ...
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bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 5 L-malic acid and 20 μM tetramethylrhodamine methyl ester (TMRM, Thermo Fisher) for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Fly brains were incubated with 100 nM Tetramethylrhodamine methyl ester (TMRM) (Invitrogen, I34361) for 30 min at 37 °C and washed three times with Hank’s balanced salt solution (HBSS) ...
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bioRxiv - Neuroscience 2019Quote: ... The co-cultures were stained with tetramethylrhodamine methyl ester (TMRM, Invitrogen; 250 nM) for 45 min at 37 °C ...