Labshake search
Citations for Thermo Fisher :
1301 - 1350 of 6146 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... and from the liver graft of living donors (N=4) were obtained at the time of liver transplantation and stored frozen in RNAlater solution (Invitrogen). ABCB4 pathogenic mutations were documented in all PFIC3 by genetic analysis ...
-
bioRxiv - Immunology 2023Quote: ... and stained for SARS-CoV-2 N protein using Biotin-labelled-CR3009 antibody produced in-house together with a Streptavidin-Alexa488 (S32354, Invitrogen) and cellular DNA using DAPI (10236276001 ...
-
bioRxiv - Neuroscience 2022Quote: ... the medium was changed to neural induction media (1:1 mixture of DMEM/F-12 GlutaMAX: Neurobasal media supplemented with 0.5x N-2 (Life Technologies), 0.5x B-27 (Life Technologies) ...
-
bioRxiv - Immunology 2022Quote: ... sheared gDNA of each sample was split in two duplicates and samples were incubated o/n at 65°C after mixing with an equal volume hybridization buffer (1 ml composition: 667 µl 20x SSPE (Gibco); 267 µl 50x Denhardt’s solution (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... formalin-fixed fibrotic capsule tissue samples (n=2 per group) and subcutaneous tissue samples (n=2) were placed in a solution of 2 mg/ml FITC (Invitrogen) in DMEM/F12 media (Gibco) ...
-
bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
-
bioRxiv - Biophysics 2023Quote: ... Cy3B and AF647 free dyes were prepared by hydrolyzing the corresponding NHS ( N-hydroxysuccimidyl) esters (PA63101, Cytiva, and A200006, Invitrogen) in 0.1 M sodium bicarbonate overnight ...
-
bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
-
bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
-
bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing N-terminally FLAG-tagged DOR or MOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
-
bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: ... 30 mg of tissue per sample was homogenized in N-PER Neuronal Protein Extraction Reagent (Thermo Fisher Scientific, Cat. # 87792) with protease and phosphatase inhibitors (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... stained with N-(3-triethylammonium propyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM 1-43FX, Invitrogen, Cat.-No. F35355) at 5.6 µg ml-1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 0.1M Tris-base in DEPC H2O) and 1X PBS for 5 min each before being blocked using 100 mM N-succinimidyl acetate (NHS-acetate, ThermoFisher) in NHS-acetate buffer (0.1M NaP ...
-
bioRxiv - Immunology 2023Quote: Following euthanasia, adult zebrafish heads (15 wpf, N=3) were fixed in a solution of 4% methanol-free formaldehyde (Thermofisher) in 60 mM HEPES buffer (pH 7.4 ...
-
bioRxiv - Biophysics 2023Quote: ... incubated in 10 mg/mL sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... as already described [17] and reseeded onto 0.2 mm bone slices labelled with N-hydroxysuccinimide ester-activated rhodamine fluorescent dye (ThermoFisher Scientific), at a cell density of 1x105 cells/bone slice (Boneslices.com) ...
-
bioRxiv - Plant Biology 2023Quote: ... For C-terminal fusions the CDS of PYE and OLV versions were amplified from cDNA of Fe deficient Arabidopsis WT roots with primer pairs PYE_B1 fw/PYEns_B2 rev and OLV_B1 fw/OLVns_B2 rev carrying B1 and B2 attachment sites without stop codon or from existing pDONR with N-terminal fusion (Supplemental Table S1) and transferred via Gateway cloning into pDONR207 (Invitrogen) according to the manual (Gateway ...
-
bioRxiv - Molecular Biology 2023Quote: ... The flow-through was dialysed O/N at 4°C in a SnakeSkin™ Dialysis Tubing (3.5K MWCO, Thermo Scientific) against dialysis buffer (50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™) for 3 h at 37°C ...
-
bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
-
bioRxiv - Neuroscience 2023Quote: ... A 6xHis-myc tag or GFP was attached to the N-terminus and the construct was cloned into the pSecTag2A vector (Invitrogen) in frame with the N-terminal IgK signal sequence ...
-
bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
-
bioRxiv - Biochemistry 2024Quote: ... Labeling of WT and p.R196H actin at cysteine–374 using N–(1–pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) followed established protocols (55).
-
bioRxiv - Genetics 2024Quote: ... Wild type N/TERT-2G cell line was cultured using EpiLife medium with 60 µM calcium (Gibco™, cat. #MEPI500CA), supplemented with human keratinocyte growth supplement (HKGS ...
-
bioRxiv - Microbiology 2024Quote: ... at physiological pH before being spun down and resuspended in PBS with the addition of FM 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide (Cat. no. T3163, Invitrogen). Images were taken on a Zeiss X10 light microscope and cell area was measured using CellProfiler 4.2.1 (33,34).
-
bioRxiv - Cell Biology 2024Quote: ... and pCRSIPR-Lenti-V2 by electroporation using the NEON system (Thermo Fisher, buffer N, 4 pulses, 10 ms, 1230 V). After electroporation ...
-
bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco® ...
-
bioRxiv - Microbiology 2024Quote: O/N cultures were diluted 10000-fold in BHI and 150 μl inoculated into a round bottom 96-well plate (Nunc) to initiate biofilm formation in microtiter plates ...
-
bioRxiv - Microbiology 2024Quote: ... HEK293FT (293T cells optimized for VLP production, gift from N. Manel) and OMK cells were cultivated in media DMEM (Thermofisher) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 3,4-dihydroxystyrene and 2-methoxy-4-vinylphenol concentrations were quantified using a Dionex UltiMate 3000 HPLC (Thermo Scientific) with a Discovery® HS-F5-3 column (15 cm x 2.1 mm ...
-
bioRxiv - Biophysics 2020Quote: ... Protein solutions were then allowed to fibrillate O/N at 37°C with 900 rpm shaking in a table Eppendorf shaker (Thermo Scientific). After 24 h incubation ...
-
bioRxiv - Biophysics 2019Quote: ... and subsequently cloned into CMV-mCherry-Gateway vector or Vivid Colors™ pcDNA™6.2/N-EmGFP-DEST vector (ThermoFisher Scientific) to obtain CMV-mCherry-DBNL or CMV-EmGFP-DBNL ...
-
bioRxiv - Cell Biology 2020Quote: ... Germany) followed by staining with Oregon Green 488 goat anti-rabbit IgG (cat. n. O-11038, Molecular Probes, Leiden, The Netherlands). Microglia cells were plated in astrocyte-conditioned high glucose-DMEM medium (cat n ...
-
bioRxiv - Cell Biology 2020Quote: ... vector and had a stop codon inserted at residue 90 to create the N-cTnC construct using the Phusion site-directed mutagenesis protocol (Thermo Scientific). This construct was transformed into the BL21(DE3 ...
-
bioRxiv - Cell Biology 2020Quote: ... 12×106 Sf9 cells (1ml) were transfected with 5μg of purified bacmid using 15μl of Cellfectin™ (Invitrogen, P/N 58760). After 5h incubation ...
-
bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... Xenopus tropicalis R651X and a nuclear-localized β galactosidase (n gal) lineage tracer were synthesized in vitro according to β manufacturer’s protocols (mMessage mMachine kit, Ambion). Antisense RNA probes for in situ hybridization (ISH ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Ovipositors of wild and lab-reared flies (n ≤ 10 per species) were mounted onto slides with Permount mounting medium (Fisher Scientific) and coverslips ...
-
bioRxiv - Developmental Biology 2021Quote: ... testes were collected from Dnmt3AKO and WT littermate animals at 19dpp and 25dpp (n=2 per genotype and age) and homogenized in Trizol (Life Technologies) using TissueLyser LT (Qiagen) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were separated on 4–12% NuPAGE® Bis-Tris gradient gels (1.0 mm x 12 well) (Invitrogen, Cat n°NP0321) using MOPS SDS Running Buffer (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
-
bioRxiv - Genetics 2019Quote: ... n = 4) for 24 hours and processed for RNA isolation and reverse transcription with the Cells-to-Ct Kit (Life Technologies), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... The X-Y resolution of N-SIM was calibrated to 120-150 nm using 20 nm FluoSphere beads (Thermo Scientific, F887) in 580 nm laser ...
-
bioRxiv - Bioengineering 2019Quote: ... 1.0 x105 cells were seeded in 12-well plates (n=3/condition/cell-type) and cultured in monolayer in differentiation media (StemPro Osteogenic Media; ThermoFisher Scientific) for 28 days ...