Labshake search
Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... sections were finally mounted in the anti-fade buffer (90% glycerol in PBS plus 2.5 mg/ml p-Phenylenediamine (Thermo Fisher, 417481000)) and sealed using clear nail polish in the borders.
-
bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
-
bioRxiv - Cell Biology 2020Quote: Transient transfections were performed using Lipofectamine 2000 according to the manufacturer’s instructions using 2 μg DNA for a 35 mm well (Invitrogen, Carlsbad, CA, Cat# 11668027). Plasmid ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were visualised using agarose gel electrophoresis with a 2% TAE gel containing a 1:10,000 dilution of SYBR® Safe DNA Gel Stain (Invitrogen, Carlsbad, CA, USA).
-
bioRxiv - Developmental Biology 2021Quote: A dam at E16.5 in gestation was given an intraperitoneal (IP) injection with EdU (5-ethynyl-2’-deoxyuridine) from the Click-iTTM EdU Alexa FluorTM 647 Imaging Kit (Invitrogen, Carlsbad, CA, USA) at a dosage of 0.25mg /10g body weight ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNAs from 7-d-old seedlings grown on 1/2 MS with or without 1 μM ABA were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) or Universal Plant Total RNA Extraction Kits (Spin-column)-I (BioTeke ...
-
bioRxiv - Molecular Biology 2020Quote: ... Zebrafish larvae were incubated in 1x E3-medium buffered with 4mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technologies, Carlsbad, CA, USA). Seahorse XF Cell Mito Stress kit (Agilent Technologies ...
-
bioRxiv - Genetics 2021Quote: ... Poly (A) RNA was obtained from 2 μg of total RNA using the Dynabeads Oligo (dT)25-61005 Kit (Thermo Fisher, CA, USA), with two rounds of purification ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were concentrated onto the trap column at 5 μl min-1 for 5 min with Buffer A (0.1% formic acid, 2% Acetonitrile) and infused into an LTQ-Orbitrap Elite (Thermo Scientific, San Jose CA) at 300 nl min-1 via the analytical column using a Dionex Ultimate 3000UPLC (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 67-56-1) and 2-propanol (CAS; 67-63-0) were all of OptimaTM LC/MS Grade and obtained from Fisher Scientific (Ontario, Canada). Dichloromethane (CAS ...
-
bioRxiv - Cell Biology 2021Quote: ... The mRNA levels were determined by SYBR Green dye using an ABI 7500 sequence detection system with a reaction mixture that consisted of SYBR Green 2×PCR Master Mix (Applied Biosystems, CA, USA), cDNA template ...
-
bioRxiv - Cancer Biology 2021Quote: ... SK-MEL-2 ATCC® HTB-68™) and cultured in DMEM (Mediatech, Manassas, VA, USA) with 5% FBS (Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... embryos were washed 3 times in PBV before incubation for 2 h at room temperature in the dark with anti-rabbit Alexa Fluor 594-conjugated secondary antibody (Life Technologies, Carlsbad, CA) at 1:500 dilution with 10 % goat serum in PBV ...
-
bioRxiv - Cancer Biology 2021Quote: HEPG2 cells were transfected with 2 μg of either KYAT1 overexpression vector or an empty vector using Lipofectamine 3000 (Invitrogen, Camarillo, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... cultures were centrifuged for 2 min in a microcentrifuge at room temperature and the pellet was quickly resuspended in Trizol solution (Invitrogen, Carlsbad, CA, USA). For transcriptional analyses of bacteria in infected cells ...
-
bioRxiv - Neuroscience 2020Quote: ... a secondary antibody was applied to the sections for 2 hours (AlexaFluor goat anti-mouse 594 diluted 1:400 in blocking buffer; Life Technologies, Carlsbad, CA). Sections were again washed in PBS over 30 minutes prior to the application of mounting media and coverslips.
-
bioRxiv - Developmental Biology 2020Quote: ... sections were rinsed in 0.02M KPBS and incubated for 2 hr at room temperature in blocking buffer containing goat anti-rabbit Alexa-Fluor conjugated secondary antibodies (Life Technologies, Carlsbad, CA). Sections were coverslipped using ProLong antifade mounting medium (Life Technologies ...
-
bioRxiv - Synthetic Biology 2021Quote: ... HeLa and LinXe cells were maintained in DMEM containing 10% fetal bovine serum (Gemini Bio-Product, CA, USA) and 2 mM GlutaMax (Thermofisher Scientific, MA, USA). Fugene 6 transfection reagent (Promega ...
-
bioRxiv - Cell Biology 2020Quote: HK-2, and ACHN cells were cultured in DMEM (Hyclone, ThermoScientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) at 37 °C in 5% CO2 ...
-
bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific, Carlsbad, CA, USA) and 20 μL of a 50% slurry of sepharose Protein A (Rockland Immunochemicals ...
-
bioRxiv - Immunology 2021Quote: ... rVSV-ΔG/SARS-2-S*-luciferase pseudovirus was generated by transfecting BHK21/WI-2 cells with pcDNA expressing codon-optimized full-length SARS-CoV-2 S protein (GenBank accession number: MN908947) using Lipofectamine™ 2000 transfection reagent (Invitrogen, Carlsbad, CA). Transfected cells were then infected with rVSV-ΔG/G*-luciferase 24 h later ...
-
bioRxiv - Immunology 2020Quote: Seventy-six consecutive patients with SARS-CoV-2 infection confirmed through reverse transcriptase-polymerase chain reaction (RT-PCR, Life Technologies, Carlsbad, CA, USA) from March 16 through March 27 ...
-
bioRxiv - Molecular Biology 2023Quote: About 0.5 μg protein as tryptic peptides dissolved in 2% acetonitrile (ACN) and 0.5% formic acid (FA) were injected into an Ultimate 3000 RSLC system (Thermo Scientific, Sunnyvale, CA, USA) connected online to Orbitrap Eclipse mass spectrometer (Thermo Scientific ...
-
bioRxiv - Genetics 2024Quote: ... The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco, Thermo Fisher Scientific, CA, USA).
-
bioRxiv - Genetics 2024Quote: ... The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco, Thermo Fisher Scientific, CA, USA).
-
bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
-
bioRxiv - Immunology 2024Quote: ... Magnetic bead removal and the evaluation of CAR expression on T cells by flow cytometry were performed on day 6 by staining with a goat anti-mouse F(ab’)2 antibody (Invitrogen, Carlsbad, CA, USA). CART cells were harvested and cryopreserved on day 8 for future experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were washed three times in PBS and incubated for 2 hours in a goat anti-rabbit Alexa Fluor 555 antibody (A21429, 1:500, Invitrogen, Carlsbad, CA, USA), they were washed in PBS three more times ...
-
bioRxiv - Immunology 2023Quote: ... Cells were transfected using 2 μg of pNF-kB-Luc and 10 ng of pRL-TK-Luc per well using Lipofectamine (Invitrogen, San Diego, CA) for 24 hrs ...
-
bioRxiv - Biochemistry 2022Quote: ... Lyophilized peptide mixtures were dissolved in 0.1% formic acid and loaded onto a 75 μm × 50 cm PepMax RSLC EASY-Spray column filled with 2 μM C18 beads (ThermoFisher San, Jose CA) at a pressure of 800 BAR ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... Tissues from L1/2-L6/S1 and Ca1/2-Ca14/15 of same mouse were pooled and served as a single sample stored into RNAlater® Reagent (Invitrogen, Carlsbad, CA) for minimum 2 days at -80°C (n = 7 mice/genotype ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were infected with a lentiviral vector containing wild-type WWOX and selected with 2 mg/mL G418 (Gibco:11811031, San Diego, CA). To induce KRAS expression in 266-6 cells 2 μg/mL of doxycycline (Tamar Laboratory Supplies ...
-
bioRxiv - Immunology 2023Quote: ... Mouse T cells were maintained in RPMI-1640 supplemented with sodium pyruvate and 2-mercaptoethanol (both from Life Technologies Corporation, Carlsbad, CA, USA). All media was supplemented with 10 % fetal bovine serum ...
-
bioRxiv - Microbiology 2023Quote: ... The following custom TaqMan gene expression assay IDs were used to assess the expression of RSV N and SARS-CoV-2 N genes: ARU66XH and APNKYWD (Applied Biosystems, CA, USA). A no template control was included in each run ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: We used para-chlorophenylalanine (PCPA, a.k.a. DL-4 Chlorophenylalanine, fenclonine) (CAS: 7424-00-2, ThermoFischer, Acros Organics, Geel, Belgium; stored at 4°C), an inhibitor of the enzyme tryptophan hydroxylase (TPH) ...
-
bioRxiv - Molecular Biology 2024Quote: ... PCR products were visualized on a 2% agarose gel to ensure robust amplification and treated with ExoSAP-IT (Affymetrix, Santa Clara, CA, US) using 1 µL of ExoSAP-IT and 7 µL of PCR product under the following conditions ...
-
bioRxiv - Physiology 2024Quote: ... the cell suspension was centrifuged at 300xg for 5 minutes before being resuspended in a pre-warmed neurobasal medium supplemented with 2% B27 supplement (Gibco Invitrogen, CA, USA), 0.25% GlutaMAX (Gibco Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: cDNA synthesis was performed using 2 µg total RNA with the High-Capacity RNA-to-cDNA Kit (Applied Biosystems; Foster City, CA, USA). Quantitative PCR (qPCR ...
-
bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
-
bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
-
bioRxiv - Biophysics 2022Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Catalog No. 22980) and Sulfo-NHS (N-hydroxysulfosuccinimide, Catalog No. 24510) were purchased from Thermo Scientific (Waltham, MA, US). Phosphate-Buffered Saline (PBS ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
-
bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
-
bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
-
bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
-
bioRxiv - Neuroscience 2024Quote: ... 5% CO2 in GFD medium supplemented with 5 mM L-glucose and 1 mM 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG; Invitrogen). Finally ...
-
bioRxiv - Cell Biology 2024Quote: Cells were transfected and 24h later treated with 250µM or 500µM of glucose fluorescent analogue 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (#N13195, Thermofisher) in Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Cell Biology 2020Quote: ... Differentiation was started when the cells were 80-90% confluent with medium containing Roswell Park Memorial Institute (RPMI) 1640 supplemented with GlutaMAX (Thermo Fisher Scientific), 0.5% B27 supplement (Thermo Fisher Scientific) ...