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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: RNA was isolated from day 2 LPS blasts seeded with sorted B cells using TRIzol reagent following the manufacturer’s instructions (Life Technologies, Carlsbad, CA). Genomic DNA was extracted from flow-purified naïve and GC B cells using lysis buffer (0.2% SDS ...
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bioRxiv - Neuroscience 2022Quote: ... except that they also received 2 injections of 10 mg/kg 5-ethynyl-2’-deoxyuridine (EdU) from the Click-iT EdU Alexa Fluor 647 imaging kit (Invitrogen, Carlsbad, CA), the day after DOX injection (4 DPN ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed with PBS three times and then incubated for 2 h with 1:1000 goat anti-mouse Alexa 488 (Catalogue no. A11029, Invitrogen, CA, USA) and 1:1000 goat anti- rabbit Alexa 59 (Catalogue no ...
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bioRxiv - Neuroscience 2022Quote: Complementary DNA (cDNA) was obtained from 2-5µg total RNA using the SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, EUA) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: The HeLa cell line (ATCC-CCL-2) was obtained from ATCC and cultured with complete DMEM (high glucose) (ThermoFisher Scientific, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... Detection of SARS-CoV-2 was performed by TaqMan-based RT-qPCR using SuperScript III Platinum One-Step RT-qPCR Kit (Invitrogen, Carlsbad, CA) and E-Sarbeco primers and probe as previously described30 ...
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bioRxiv - Biochemistry 2024Quote: ... and only samples with 260/280 absorbance ratio between 1.8 and 2 were used for reverse transcription using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... and 200 nM of the designated primers (Table 2) on a 7500 Fast Real-time PCR system (Applied Biosystems, Carlsbad, CA, USA). Amplification was initiated at 95 °C for 20 sec and was followed by 40 cycles of 95 °C for 3 sec and 60 °C for 30 sec ...
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bioRxiv - Physiology 2024Quote: ... the cell suspension was centrifuged at 300xg for 5 minutes before being resuspended in a pre-warmed neurobasal medium supplemented with 2% B27 supplement (Gibco Invitrogen, CA, USA), 0.25% GlutaMAX (Gibco Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation in response to GNE-3511 treatment was assessed by 5-ethynyl-2′-deoxyuridine (EdU) incorporation using the Click-iT EdU Alexa Fluor 488 cell proliferation assay kit (Invitrogen, Carlsbad, CA). CAL33 cells were seeded in µ-Plate 24 Well plates (Ibidi GmbH ...
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bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
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bioRxiv - Microbiology 2022Quote: ... PAFB was labelled with the green fluorophore 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylenediamine hydrochloride (BODIPY™ FL EDA, Invitrogen, Waltham, MA, USA) as described (32).
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bioRxiv - Biochemistry 2021Quote: Total RNA of cells was extracted after treatment of cells with sertraline hydrochloride for 72 h using TRIzol reagent (Cat no. 15596-018, Ambion, Life Technologies, USA). Complementary DNA (cDNA ...
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bioRxiv - Systems Biology 2023Quote: ... Val-Tyr-Val, methoxyamine hydrochloride (MeOX), N-methyl-N-(trimethylsilyl)-trifluoroactamide (MSTFA), and pyridine (Anhydrous, 99.8%) were all purchased from Fisher Scientific (Hampton, NH, USA). Fatty acid methyl esters (FAMEs ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in CAS block for 1 hour (Invitrogen, Carlsbad, CA), and incubated in primary antibodies (diluted in CAS Block ...
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bioRxiv - Neuroscience 2020Quote: ... (Invitrogen, Carlsbad, CA), supplemented with B27 (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... (Invitrogen, Carlsbad, CA).
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bioRxiv - Immunology 2020Quote: (Gibco, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... CA-beads (Invitrogen) and 19% Polyethylene glycol PEG ...
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bioRxiv - Cell Biology 2024Quote: ... (Gibco, Carlsbad, CA) and 10 ng/mL EGF (Peprotech)86 ...
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bioRxiv - Biophysics 2021Quote: ... the LNPs were formulated with a PolyA cargo and concentrated after dialysis to ∼90 mg/mL of lipid by Amicon ultrafiltration with a 30 kDa MWCO (Thermo Scientific). Each batch of LNPs was characterised prior to use to determine particle size ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were separated with an increasing gradient of organic solvent (0-60 % acetonitrile in 90 min) on an analytical column (Acclaim PepMap column (Thermo Scientific), 2 μm particle size ...
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bioRxiv - Biophysics 2020Quote: ... The SH-SY5Y human neuroblastoma cells at a confluency of 80-90% were transfected with the plasmid and Lipofectamine LTX according to the manufacturer’s protocol (Thermo Fisher Scientific). The cells were incubated under 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... vector and had a stop codon inserted at residue 90 to create the N-cTnC construct using the Phusion site-directed mutagenesis protocol (Thermo Scientific). This construct was transformed into the BL21(DE3 ...
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bioRxiv - Genetics 2021Quote: ... but extending cDNA synthesis at 42°C for 90 min in the presence of 20 U RiboLock RNase Inhibitor (EO0381; Thermo Scientific). Prior to quantitative real-time PCR (qRT-PCR) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated at room temperature for 90 min in a cocktail of the following secondary antibodies: goat anti-rabbit Alexa Fluor 555 (1:500; Molecular Probes, Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Spleen tissue was gently pressed with the flat end of a 5-ml syringe to release the splenocytes into cell cultural medium that contained 90% RPMI-1640 (Cat#11875, Life technologies) supplemented with 10% heat-inactivated fetal bovine serum [HI-FBS] (Cat#SH30071.03 ...
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bioRxiv - Bioengineering 2021Quote: ... These proteins were then reduced at 90°C for 10 min and loaded into a 4-12% bis-tris gel (Life Technologies). This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cells were cultured on 35 mm dishes until reaching 80-90% confluency and total RNA was isolated using the TRIzol reagent according to manufacturer’s instructions (Thermo Fisher Scientific). mRNA quality was controlled by running 1% agarose gels and checking the presence of sharp ...
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bioRxiv - Biochemistry 2021Quote: ... and proteins were separated using a gradient of solvent B (99% acetonitrile with 0.1% formic acid) from 5 to 65% in 90 minutes using an EASY-nLC 1000 system (Thermo Fisher Scientific). Ion fragmentation was conducted using collision-induced dissociation (CID) ...
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bioRxiv - Systems Biology 2021Quote: ... Steady state and migrated LC were processed through fluorescence-activated cell sorting (FACS) and Drop-seq or cryopreserved in 90% FBS (Gibco, UK), 10% DMSO (Sigma ...
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... Explants were passaged at 70% confluency and cryopreservation stocks were prepared from P0 passage times in 90% BS 10% DMSO (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... cushion at 20,000 x g for 1 h in a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (Thermo Fisher Scientific). Viral stock titers were titered on MRC-5 (TB40/EFb-eGFP ...
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bioRxiv - Microbiology 2021Quote: ... Cell pellets were resuspended in 1 ml trace-metal grade 35% nitric acid (HNO3) and digested at 90°C for 1 hour in a high-density polyethylene scintillation vial (Fisher Scientific). Digested bacterial cells were diluted at a ratio of 1:10 in reagent-grade water prior to analysis ...
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bioRxiv - Microbiology 2020Quote: ... in 20 mL of 0.2 μm-filtered lake water with the addition of 0.8 M urea in closed 50-mL glass serum bottles with agitation (90 rpm, orbital shaker MaxQTM 2000, Thermo Fisher Scientific) at room temperature and under natural light cycles for 12 days ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... LGm and OXPHOSm were plated in IBIDI μ-Slide 8 Well ibiTreat at a density of 6000 cells/well and after 24 h cells were incubated for 90 minutes with MitoTracker Red CMXRos (Molecular Probes, Invitrogen) at 100 nM ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to 90% confluence on a 10 cm plate and were treated with 0.1 μg/mL KaryoMax Colcemid (Thermo Fisher Scientific) for 16 h overnight ...
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bioRxiv - Neuroscience 2022Quote: ... extracted samples were denatured at +90°C for 5 min in a buffer containing 1X LDS (NuPAGE® LDS sample buffer, Invitrogen) and DTT 1X (NuPAGE® sample reducing agent ...
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bioRxiv - Developmental Biology 2022Quote: ... The hiPSC were passaged at 70-90% confluency by washing with DPBS and then adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 °C for 4 mins ...
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bioRxiv - Developmental Biology 2022Quote: ... The hiPSC were passaged at 70-90% confluency by washing with DPBS and then adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 °C for 4 mins ...
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bioRxiv - Developmental Biology 2022Quote: ... The hiPSC were passaged at 70-90% confluency by washing with DPBS and then adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 °C for 4 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... the tryptic peptides were separated by increasing concentrations of 80% ACN/0.1% formic acid at a flow of 250 nL/min for 90 min and analyzed with a QExactive HF mass spectrometer (Thermo Scientific). The instrument was operated in the data dependent acquisition mode to automatically switch between full scan MS and MS/MS acquisition ...
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bioRxiv - Bioengineering 2021Quote: ... Caco-2 cells were seeded in a tissue culture treated 12-well plate and grown to 90–100% confluence by observation (Fisher Scientific). The medium was replaced every other day.
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bioRxiv - Neuroscience 2022Quote: ... cDNA of N-terminal Flag-tagged wild-type human eIF4A1 was amplified by PCR and subcloned into the pLenti-CMV-Puro-DEST (w118-1) vector 90 using the Gateway cloning system (Thermo Fisher). The TIMMDC1 ...
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bioRxiv - Developmental Biology 2022Quote: ... hESCs are obtained from WiCell (RRID:CVCL_9772) maintained and split from around 90% confluence with 1x versene (Fisher Scientific 15-040-066) for 5 minutes at RT and then split with 1:8 ratio into 6 well plates pre-coated with 1:200 diluted geltrex in DMEM/F12 medium (Thermo Scientific A1413201 ...
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bioRxiv - Developmental Biology 2022Quote: ... The hiPSC were passaged at 70-90% confluency by washing with DPBS and then adding Accutase cell dissociation reagent (ThermoFisher A1110501) and incubating at 37 °C for 4 mins ...
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bioRxiv - Immunology 2021Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37◦C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
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bioRxiv - Immunology 2020Quote: ... MoDCs (50,000/well) were loaded for 60–90 min at 37°C with peptide and labelled with Cell Trace Violet (Thermo Fisher) to distinguish them from T cells prior to co-culturing with 50,000 T cells/well in a 96 well plate for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... The fruit were dried off and two fruit were placed into each sterile 90 mm triple-vented petri dish bottom or lid (Thermo Scientific). The petri dish lids and bottoms with fruit were placed on trays sterilised with 70% ethanol ...
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bioRxiv - Genomics 2021Quote: ... cells were cultured on gelatin-coated 6-wells in 60% BRL conditioned medium to 70-90% confluency and transfected with Lipofectamin3000 (Invitrogen L3000008) according to the supplier protocol with 2 μg donor vector and 1 μg Cas9/guide vector ...