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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 90 minutes with Alexa Fluor 488 goat anti-rabbit IgG (1:500 dilutions; Invitrogen, Cat.#: A11011). After that ...
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bioRxiv - Genomics 2020Quote: ... Ligation reactions were transformed into One Shot™ Stbl3™ chemically-competent E.coli cells (42°C heat-shock, 90 seconds; Invitrogen C737303) and positive clones were selected by Sanger sequencing ...
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bioRxiv - Physiology 2020Quote: In situ hybridization was performed on primary cell cultures seeded on chamber slides at 90% confluency (106 cells/well) (Thermofisher Scientific, UK). Cells were fixed in 4% PFA for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples are frozen for at least 10 min at −80°C and then immediately centrifuged for 90 min at 4°C and 13000 rpm (Heraeus™ Biofuge fresco, Thermo Scientific). After centrifugation three different stable phases are obtained ...
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bioRxiv - Cancer Biology 2020Quote: ... as per the manufacturer’s instructions and incubated with 10 μg primary antibodies overnight at 4°C followed by 90 minutes incubation with 35 μl protein A/G agarose plus (50% slurry) (ThermoFisher Scientific 20421). Immune pellets were washed 5 times in PBS containing 1% Triton-X100 and analyzed by SDS-PAGE followed by either immunoblotting or mass-spectrometry (LC/MS-MS) ...
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bioRxiv - Biochemistry 2021Quote: ... To stop the digestion reaction the samples were heated for 1 min to 90°C and the concentration was determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific) resulting in a protein concentration of 0.94 μg/μL ...
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bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency and cell surface proteins were biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher Scientific, #21330) at 0.5 mg/ml in HBSS (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 20 min wash at 90% followed by 50 min re-equilibration) and directly spray onto the mass spectrometer using EasySpray source (Thermo Fisher Scientific). Mass spectrometer was set to collect one MS1 scan (Orbitrap ...
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bioRxiv - Biophysics 2020Quote: Multiple colonies from the agar plates were picked with the aid of sterilized cotton-tipped applicators and inoculated in 5 mL of BB10 (90% Brucella Broth, Millipore Sigma + 10% Fetal Bovine Serum, Gibco™) to grow overnight cultures ...
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bioRxiv - Cell Biology 2021Quote: ... The grids were blotted with a single blotting paper for 5.5 s at a blot force of −2 in 90% humidity at 4°C before being plunge-frozen in liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2020Quote: ... The electrophoresis of amplified products was performed on 6% acrylamide gels at 160 Volts for 30 minutes (Thermo Electron Electrophoresis EC250-90, Thermo Fisher Scientific). The results were visualized under UV light using UVITEC Imaging Systems (Cleaver Scientific ...
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bioRxiv - Microbiology 2020Quote: ... CHO cells were seeded on coverslips in 24-well plates until reach 70-90% of confluency and were transfected using Lipofectamine® LTX (Invitrogen USA) according to manufacturer protocol with 1 µg of each plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... — frontal cortices were bilaterally dissected on ice and immediately submerged in a cryopreservation medium containing 90% of fetal bovine serum (Gibco™ A3160601) and 10% DMSO (Sigma-Aldrich ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... LGm and OXPHOSm were plated in IBIDI μ-Slide 8 Well ibiTreat at a density of 6000 cells/well and after 24 h cells were incubated for 90 minutes with MitoTracker Red CMXRos (Molecular Probes, Invitrogen) at 100 nM ...
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bioRxiv - Cell Biology 2021Quote: ... The electrophoretic run was performed for 90 min at 125 volts with MOPS as the running buffer (Thermo Fisher Scientific, NP0001, US) (2017 ...
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bioRxiv - Biochemistry 2022Quote: The IMR-90 cells (JCRB cell bank #JCRB9054) were cultivated in four T75 flasks containing 30 mL of Minimum Essential Media (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 20 min wash at 90% followed by 50 min re-equilibration) and directly spray onto the mass spectrometer using EasySpray source (Thermo Fisher Scientific). Mass spectrometer was set to collect one MS1 scan (Orbitrap ...
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bioRxiv - Plant Biology 2022Quote: ... the acidic protons were subsequently derivatized with 90 μL of N-methyl-N-trimethylsilyltrifluoroacetamide plus 1% trimethylchlorosilane (Thermo Fisher Scientific, Waltham, MA) at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: RD cells grown in 90% confluency were infected with HCoV-HKU1 (108 RNA copies per ml) in Dulbecco’s MEM (Thermo Fisher Scientific, Poland) supplemented with 3% fetal bovine serum (heat-inactivated ...
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bioRxiv - Microbiology 2020Quote: ... Hindgut contents were washed with ice-cold TU three times at 3,000 rpm in an Eppendorf microcentrifuge for 90 seconds and then lysed in 1mL of TRIzol™ Reagent (Thermo Fisher Scientific). RNA was isolated per the manufacturer’s protocol and treated with TURBO™ DNase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: HEK293 adherent cells grown to a confluency of 70–90% were transfected using a Lipofectamine 3000 Reagent Kit (L3000075, Invitrogen, Waltham, MA). Transfection procedures were adapted from the product manual ...
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bioRxiv - Cancer Biology 2021Quote: ... were surgically removed from resected liver lobes from ICC patients and immersed in a complete medium containing 90% Dulbecco’s Modified Eagle medium (Gibco, Gaithersburg, MD, USA) and 10% fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2020Quote: 90 μL CFE semi-continuous reactions were performed using 3.5 kDa MWCO 96-well plate dialysis cassettes (Thermo Scientific, Rockford, IL, USA) in 2 mL microcentrifuge tubes with 1.4 mL dialysis buffer solution ...
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bioRxiv - Biophysics 2020Quote: ... 60 or 90 min post treatment using 4% paraformaldehyde and next stained with primary antibodies for STIM1 (#PA1-46217, Thermo Fisher Scientifc), Orai1 (#NBP1-75523 ...
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bioRxiv - Microbiology 2020Quote: Each sample was analyzed using an Q-LTQ-OT tribrid mass spectrometer (Orbitrap Fusion Lumos) during a 90 min nano-liquid chromatography using a Dionex UltiMate 3000 RSLCnano system (Thermo Fisher Scientific). MS parameters differed for the analysis of phosphopeptides enriched and unenriched sample ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 6 min wash at 90% followed by 25 min re-equilibration) and directly sprayed onto the Orbitrap Fusion using EasySpray source (Thermo Fisher Scientific). Mass spectrometer was set to collect one MS1 scan (Orbitrap ...
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bioRxiv - Molecular Biology 2023Quote: ... the monomer solution was removed and retinas were incubated in 90 μl of monomer solution with the addition of ammonium persulfate (APS, 17874, Thermo Fisher Scientific) and tetramethylethylenediamine (TEMED ...
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bioRxiv - Genomics 2023Quote: ... A 10 μl aliquot of suspended nuclei was diluted in 90 μl APB (1:10 dilution) and manually counted on a hemocytometer with Trypan Blue staining (Thermo Scientific T8154). The stock nuclei suspension was then diluted to a concentration of 2,857 nuclei/μl in APB ...
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bioRxiv - Biophysics 2023Quote: ... samples were stained for 90 min with 1.65 x 10−7 M Alexa Fluor 488 phalloidin (Invitrogen, Life Technologies Corporation, Oregon, USA) in 1x DPBS ...
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bioRxiv - Microbiology 2023Quote: ... 20 min wash at 90% followed by 50 min re-equilibration) and directly sprayed onto the mass spectrometer using EasySpray source (Thermo Fisher Scientific). Mass spectrometer was set to collect one MS1 scan (Orbitrap ...
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bioRxiv - Physiology 2023Quote: ... Ultra-thin sections (90 nm) were obtained with an ultra-microtome for morphological examination using a transmission electron microscope (Tecnai G2, Thermo Fisher Scientific). Cremaster muscles with thrombi were mounted on an SBF pin ...
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bioRxiv - Genomics 2023Quote: ... the samples were heated on the heat block at 90°C for 5 minutes in the 50% denaturation buffer with aluminum sealing tapes (Thermo Scientific 232698) on the inlet and outlet of the custom chamber ...
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bioRxiv - Cell Biology 2022Quote: ... cell counting was performed with trypan blue staining to assess cell viability (>90%) using an automated cell counter (CountessTM, Thermo Fisher Scientific). Conditioned medium was then collected and centrifuged for 10 min at 300 g and 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were maintained at 37°C in a > 90% relative humidity incubator with 5% CO2 (HeraCell 150i, Model No. 1335, Thermo Fisher Scientific). Routine subculturing was performed with 0.25% Trypsin-EDTA (Gibco® ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were used at 80% to 90% confluency for all the experiments with cell viability ranging from 95 to 98% (Countess II FL, Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Tryptic digests were analyzed using a standard 90-minute LC gradient on the Thermo Q Exactive Plus (Thermo Fisher, Waltham, MA, USA) mass spectrometer.
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bioRxiv - Neuroscience 2023Quote: ... Neuron-cancer cocultures were quantified for neuronal outgrowth by identical image generation and processing with additional pre-incubation (3 drops/mL for 90 min at 37◦C) of NucRed Dead 647 ReadyProbes™ Reagent (TO-PRO-3 iodide) (Invitrogen, #R37113) for dead cells and NucBlue Live ReadyProbes™ Reagent (Hoechst 33342 ...
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bioRxiv - Cancer Biology 2023Quote: ... were denatured for 10 min at 70°C then reverse transcribed at 37°C for 90 min in a buffer containing Superscript II reverse transcriptase (Thermo Fisher Scientific), DTT (10 mM) ...
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bioRxiv - Systems Biology 2023Quote: ... then held at 90% B for 9 minutes at a flow rate of 300 nl/min delivered by an Easy1200 nLC system (Thermo Fisher Scientific). All MS1 spectra were collected with orbitrap detection at a 70,000 resolution and a scan range from 300 to 1,500 m/z ...
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bioRxiv - Neuroscience 2023Quote: ... On the second day slices were washed 3 × PBS with 0,3% Trition X-100 and incubated for 90 minutes with secondary antibodies conjugated with anti-mouse AlexaFluor 555 (1:500, Invitrogen, A31570, RRID:AB_2536180) and/or anti-rabbit AlexaFluor 555 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... discs were incubated at room temperature for 90 min with the appropriate Alexa-fluor secondary antibodies (Invitrogen #’s A11035, A48255, or A48265) diluted 1:200 in PBSTx and 5% goat serum ...
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bioRxiv - Microbiology 2024Quote: ... and/or hemin (5 µM) and vortexed for (30 s) before transferring 90 µL to a coverslip with an attached Gene Frame (Thermo Scientific, AB0577) and incubating for 30 min (RT ...
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bioRxiv - Immunology 2024Quote: ... medium was aspirated and 30 µg of plasmid in 1.5 ml of Opti-MEM was mixed with 90 μl lipofectamine 2000 (Invitrogen, Cat. #11668-019) (i.e ...
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bioRxiv - Systems Biology 2024Quote: ... About 2 µg per fraction in 1% formic acid was analyzed in 90 min by LC-MS on an Orbitrap Ascend (Thermo Fisher Scientific) using a Real-Time-Search MS3 method25 ...
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bioRxiv - Immunology 2024Quote: ... and 20 min wash at 90% acetonitrile followed by re-equilibration for 50 min) and directly sprayed onto the mass spectrometer using EasySpray source (Thermo Fisher Scientific). The mass spectrometer was optimized to collect one MS1 scan (Orbitrap ...
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Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brainbioRxiv - Neuroscience 2024Quote: ... Slices were then washed three times with PBS for 5 minutes and incubated for 90 minutes in secondary Alexa 594 1:1000 (Thermo Fisher Scientific). Slices are washed three times with PBS for 5 minutes each ...
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bioRxiv - Physiology 2024Quote: ... At 90-100% confluency differentiation was initiated by changing the medium to α-MEM Glutamax (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 2% FBS ...
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bioRxiv - Neuroscience 2024Quote: A single DRG from 90 organ donors was sectioned at 20μm onto SuperFrost Plus charged slides (Fisher Scientific; Cat 12-550-15). The donors were randomly selected from our tissue bank as part of routine tissue morphology checking as part of our quality control process ...
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bioRxiv - Biophysics 2024Quote: ... Grids were blotted (1.0–1.5 s) at room temperature and 90% humidity and plunge froze into liquid ethane on a Vitrobot Mark IV (FEI, Thermo Fisher Scientific). Image datasets were collected at the Pacific Northwest Cryo-EM Center (OHSU ...
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bioRxiv - Cell Biology 2024Quote: Human iPSCs were grown in 6-cm cell culture dish until reached 80-90% confluence and were treated with 0.1 ug/mL Colcemid (Cat. #15212012, KaryoMAX Colcemid, Gibco, Thermo Fisher Scientific) and 0.5 uM Wee1 inhibitor (PD0166285 ...