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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were used at 80% to 90% confluency for all the experiments with cell viability ranging from 95 to 98% (Countess II FL, Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... The iPSCs were passaged every 4 - 5 days when it reached 80 - 90% confluency using the StemPro® EZPassage™ disposable stem cell passaging tool (23181-010, Life Technologies). The cells were passaged as small clumps with a 1:6 split ratio and cultured with daily medium change ...
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bioRxiv - Biophysics 2023Quote: ... They were grown at 37 ◦C in an atmosphere of 5% CO2 until 90 % confluence in complete DMEM culture medium (from GIBCO BRL; Life Technologies, Paisley, Scotland) supplemented with 1% glutamine ...
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bioRxiv - Plant Biology 2023Quote: ... Tissue was grounded in liquid nitrogen and combined with 500 μl of pre-chilled 90% methanol (A452-1, Fisher Scientific, Hampton, NH, USA) vortexed and finally incubated for 4h on ice ...
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bioRxiv - Pathology 2023Quote: Slides were incubated at 90°C for 20 minutes in High pH IHC Antigen Retrieval Solution (Thermo Fisher Scientific Catalog Number 00-4956) and allowed to cool to room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The iPSCs were passaged every 4 - 5 days when it reached 80 - 90% confluency using the StemPro® EZPassage™ disposable stem cell passaging tool (23181-010, Life Technologies). The cells were passaged as a small colony with a split ratio of 1:6 and cultured by changing the medium every day ...
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bioRxiv - Evolutionary Biology 2023Quote: ... muscarius was isolated from Mycotal (Koppert, NL) and spread on Sabouraud dextrose agar (SDA) in 90 mm triple vented Petri dishes (Fisher Scientific, Loughborough, UK). Isolation and production of conidia involved incubation in darkness at 23±1 °C on SDA plates for 10-14 days ...
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bioRxiv - Cell Biology 2023Quote: ... Pericytes were passaged at 60%-90% confluence by washing with Dulbecco’s phosphate buffered saline without magnesium or calcium (DPBS-/-, ThermoFisher Scientific, cat.#14190-144) prior to treatment with TrypLE Express (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... coated vessels.90–92 The Matrigel coating was done by diluting the stock Matrigel 1:50 with KnockOut DMEM (Thermo Fisher Scientific; 10829018) and incubating at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... in LC-MS grade water (solvent A) and 0.1 % (v/v) formic acid in 90% (v/v) LC-MS-grade acetonitrile (solvent B; Fisher Scientific, Hampton, NH, USA) at a flow rate of 0.4 mL/min and column temperature of 40 °C ...
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bioRxiv - Cell Biology 2024Quote: IMR-90 primary human lung fibroblasts (ATCC, Manassas, VA; #CCL186) were cultured in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Waltham, MA; #12430054) supplemented with penicillin-streptomycin (5,000 U/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Cell Biology 2020Quote: ... with a 220-min gradient from 10 to 30% ACN-0.2% FA and a 600-nl/min flow rate on a Easy nLC-1000 connected to an Orbitrap Fusion (Thermo Fisher Scientific, San Jose, CA). Each full MS spectrum acquired at a resolution of 240,000 was followed by tandem-MS (MS-MS ...
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bioRxiv - Genetics 2021Quote: ... cDNA was synthesized by incubating RNA for 2 h at 37 C using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). Afterwards ...
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bioRxiv - Biochemistry 2020Quote: ... the reaction mixtures with No.1 set of primers were heated at the optimized temperature 56.5°C for 100 min (2 min per cycle) in StepOne™ System Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), while the reaction mixtures with No.2 set of primers were heated at 55°C for 60 min (30 s per cycle) ...
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bioRxiv - Plant Biology 2020Quote: cDNA was synthesized from 2 μg of RNA using random primers in 20 μL reaction with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Physiology 2022Quote: ... and a final step at 72 °C for 2 min was used in a GeneAmp PCR system 2700 thermal cycler (Applied Biosystems, Foster City, CA, USA). The PCR products were resolved on 1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... S100 Calcium-binding protein A7 (S100A7) and human β-defensin 2 (DEFB4A) were amplified by using TAQMAN Gene Expression Assays (Applied Biosystems, Foster City, CA). TaqMan probes were grafted with a fluorophore (FAM ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and incubated for 2 hours at RT with Alexa Fluor 488 donkey anti-rabbit (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 555 donkey antimouse (1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s instructions and sequencing was performed on a 3730xl Genetic Analyzer for cohort 1 or 3130xl Genetic Analyzer for cohort 2 (Applied Biosystems, Foster City, CA, USA). Sequences were then analyzed using the Sequencing analysis V5.3.1 and the SeqScape V2.6 software (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1% formic acid) on an Acclaim PepMap RSLC 75 μm x 25cm column packed with C18 particles (2 μm, 100 Å) (Thermo Scientific, San Jose, CA). The peptides were ionized using a stainless steel nano-bore emitter (#ES542 ...
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bioRxiv - Cell Biology 2022Quote: ... with a 56-min gradient from 10 to 30% ACN-0.2% FA and a 600-nl/min flow rate on an Easy nLC-1000 connected to an Orbitrap Fusion (Thermo Fisher Scientific, San Jose, CA). Each full MS spectrum acquired at a resolution of 60,000 was followed by tandem-MS (MS-MS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The eluted peptides were ionized under high voltage (2 kV) and analyzed online using a LumosTM mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). The initial MS spectrum (MS1 ...
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bioRxiv - Immunology 2023Quote: ... Each sample was injected onto a PepMap C18 trap column (75 μM X 2 cm, 3 μM, 100 Å, Thermo Fisher Scientific, San Jose, CA) at 5 μL/min for 6 min using 0.05% trifluoroacetic acid (TFA ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s instructions and sequencing was performed on a 3730xl Genetic Analyzer for cohort 1 or 3130xl Genetic Analyzer for cohort 2 (Applied Biosystems, Foster City, CA, USA). Sequences were then analyzed using the Sequencing analysis V5.3.1 and SeqScape V2.6 software (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... with a 56-min gradient from 10 to 30% ACN-0.2% FA and a 600-nl/min flow rate on a Easy nLC-1000 connected to an Orbitrap Fusion (Thermo Fisher Scientific, San Jose, CA). Each full MS spectrum acquired at a resolution of 60,000 was followed by tandem-MS (MS-MS ...
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bioRxiv - Molecular Biology 2023Quote: ... this latter was performed on 1 ng of genomic DNA (gDNA) of each organism listen in Table 2 by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... with a 56 min gradient from 10 to 30% ACN-0.2% FA and a 600 nl/min flow rate on an Easy nLC-1200 connected to an Exploris 480 (Thermo Fisher Scientific, San Jose, CA). Each full MS spectrum acquired at a resolution of 120,000 was followed by tandem-MS (MS-MS ...
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bioRxiv - Immunology 2024Quote: RNA from BMDCs after treatments at different time points (2-8 hr) was extracted using the MagMAX-96 Total RNA Isolation Kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in 500 μL of primary antibody solution containing rat anti-alpha-tubulin YL1/2 (MA1-80017, Invitrogen, CA, SAD, diluted 1:500) overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... with a 56-min gradient from 10 to 30% ACN-0.2% FA and a 600-nL/min flow rate on a Easy nLC-1200 connected to a Exploris 480 (Thermo Fisher Scientific, San Jose, CA). Each full MS spectrum acquired at a resolution of 120,000 was followed by tandem-MS (MS-MS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in 500 μL of primary antibody solution containing rat anti-alpha-tubulin YL1/2 (MA1-80017, Invitrogen, CA, SAD, diluted 1:500) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Nonspecific binding was blocked by incubation with CAS block (Life Technologies, CA, USA) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... CA)9 were cultured in DMEM/F12 supplemented with N2 (Invitrogen, Carlsbad, CA), murine fibroblast growth factor-2 (20 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2024Quote: In vivo labeling of tumor-infiltrating T lymphocytes (TILs) with 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195) was done by intravenous (retroorbital ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 2 washes of 2× SSC (Ambion AM9765) for 5min ...
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bioRxiv - Immunology 2020Quote: ... 2-mercaptoethanol (2◻×◻10-5 M, ThermoFisher), penicillin (100◻IU◻per ml ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 0.05 mM 2-mercaptoethanol (2-ME; Gibco). Mouse melanoma cell line B16 (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 mM GlutaMax supplemented with 2% B27 (Gibco) and 5% horse serum (Hyclone) ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liverbioRxiv - Immunology 2024Quote: ... 1% N-2 and 2% B-27 supplements (Gibco). MC38 cells were cultured in DMEM (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (Gibco), sodium pyruvate (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (Gibco), sodium pyruvate (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
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bioRxiv - Neuroscience 2024Quote: ... non-metabolizable glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Thermo Fisher Scientific, cat.no. 11569116). Samples were prepared as described in the section ...
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bioRxiv - Immunology 2024Quote: The glucose uptake in ILC2 was measured using the glucose analog 2-(N-(7-Nitrobenz-2-oxa- 1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher Scientific, Catalog No. N13195) which was stored at -20°C at a stock concentration of 10 mM (5 mg lyophilised powder in 1.46 mL dimethyl sulfoxide (DMSO)) ...
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bioRxiv - Neuroscience 2021Quote: ... and centrifugated during 90 min at 20000xg (4°C) for ectosomes and 100000xg for 90 min for exosomes (4°C) in a swing rotor (TH-641 Sorvall; Thermo Fisher Scientific, MA, USA). As a control ...
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bioRxiv - Immunology 2022Quote: ... chopped lung tissue was incubated at 37°C for 90 minutes (mins) in RPMI with 5% fetal bovine serum (FBS) and 150 U/ml collagenase (Life Technologies, Rockville, MD, USA). Nonadherent cells were enriched on Percoll density gradients (44/67% ...
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bioRxiv - Microbiology 2022Quote: ... in BHI broth or in mouse whole blood and transferred (90 μl/well) into a 96-well transparent-bottom white microplate (Thermo Scientific Nunc: cat. no. 165306). Infection was performed by adding 10 μl of ϕA1122 or PST phage solution (MOI = 0.01 ...