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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... HDL was labeled with Alexa568 by incubating 4.5 mg Human HDL in 90 μl 1 M sodium bicarbonate with Alexa Fluor 568 succinimidyl ester (A20003, Thermo Fisher) for 1 hr at room temperature (8:1 dye:estimated apoA1 mole ratio) ...
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bioRxiv - Cell Biology 2021Quote: ... The hiPSC-CMs were cryopreserved at differentiation d20 or d21 as previously described in a freezing medium comprising of 90% Knockout Serum Replacement (Gibco) and 10% DMSO (Brink et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM MgCl2) at 55,000 × g for 1 h in a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (ThermoFisher Scientific). The TB40r mGFP-IE-FKBP BAC was kindly provided by E ...
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bioRxiv - Systems Biology 2021Quote: ... A total of 90 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Lafayette, CO, U.S.A.) was then added to the samples and shaken at 1400 rpm for 30 min at 37 °C ...
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bioRxiv - Systems Biology 2020Quote: ... Antibodies (90-100 μg) were buffer-exchanged to 1× PBS using 7K MWCO Zeba Spin Desalting Columns (Thermo Scientific 89882), and reacted with 10 equivalent of PEGylated SMCC cross-linker (SM(PEG)2 ...
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bioRxiv - Systems Biology 2020Quote: ... One bottle of the Express Five medium (1 liter) was supplemented with 90 ml of L-Glutamine (200 mM, Invitrogen). During cultivation ...
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bioRxiv - Microbiology 2021Quote: ... Transfection was performed on 8-well glass slides at 90% confluent cells using Lipofectamine® 3000 (Cat: L3000075, Invitrogen Co.), following the manufacturer’s instructions and a total of 250 ng of DNA per well was transfected ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Microbiology 2020Quote: ... with 10% dimethyl sulfoxide (DMSO) and plated one day prior to incubation with droplets in 90% fresh medium (RPMI 1640 (Gibco) supplemented with 10% FBS and 2 mM glutamine ...
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bioRxiv - Molecular Biology 2021Quote: One plasmid containing dCasRX-FLAG-YTHDC1 in pXR002 in combination with one plasmid containing the designated guide RNA in pXR003 (see description in Plasmid Construction) were transfected into a 70-90% confluent 10-cm dish using Lipofectamine 2000 (11668030, Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 70-90% confluent Eph4 cells are incubated in media containing Lipofectamine® and DNA in Opti-MEM (Gibco, 31985-062). Eph4 cells were followed for 1-3 days and then analyzed.
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bioRxiv - Cell Biology 2020Quote: ... HEK 293T cells were seeded at 5×10^6 cells and grown to 70-90% confluence in 10 cm plates in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Labeled probes were denatured by heat for 5 min at 90 °C and then incubated with the blot in ULTRAhyp Ultrasensitive Hybridization Buffer (Invitrogen) overnight at 42 °C ...
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bioRxiv - Cell Biology 2022Quote: In CD protocol 2 hESCs at 80%-90% confluency in 6-well plates were lifted with 1 ml/well UltraPure 0.5 M EDTA (Invitrogen, 15575038), counted ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed on 8-well glass slides at 90% confluent cells using Lipofectamine® 3000 (Cat: L3000075, Invitrogen Co.), following the manufacturer’s instructions and 250 ng of plasmid DNA per well were transfected ...
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bioRxiv - Systems Biology 2022Quote: ... Samples were cryopreserved at a concentration of 107 cells per milliliter in a solution of 90% fetal bovine serum (FBS, Gibco) and 10% dimethylsulfoxide (SigmaAldrich ...
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bioRxiv - Cell Biology 2022Quote: ... MuSCs were blocked for 45 minutes at 37°C in wash media (WM: 90% Ham’s F-10 + 10% horse serum) with 10 µM Hoechst 33342 (Thermo Fisher). Cells were then stained with 0.1 mg/mL Pyronin Y (Santa Cruz sc-203755 ...
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bioRxiv - Microbiology 2022Quote: ... Samples were heated to 90°C for 10 min before being loaded on 4-12% Bis-Tris polyacrylamide gels (ThermoFisher). Each experiment was triplicated ...
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bioRxiv - Cell Biology 2022Quote: ... Samples with DNA below 12.5 ng/ul (∼90 of 512 samples) were concentrated using SpeedVac Vacuum Concentrator (Thermofisher #SPD1030A-115) for <1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated for 10 min at 90°C and loaded on a 4–20% Tris-Glycine precast gel (ThermoFisher). Protein was transferred onto a nitrocellulose membrane using the iBlot Dry Blotting System ...
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bioRxiv - Neuroscience 2021Quote: ... cells at 80-90% confluence were suspended using a pre-warmed 0.25% trypsin-EDTA solution for 5 minutes maximum (Gibco SH30236.02) and transferred to 35 mm glass bottom culture dishes (Mutsunami D1130H ...
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bioRxiv - Cell Biology 2022Quote: ... RNAi was transfected into primary astrocytes at 80-90% confluence using Lipofectamine RNAiMax (Invitrogen by Thermo Fisher, Cat. No. 56531). Knockdown efficiency was confirmed with qPCR as well as western blotting ...
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bioRxiv - Cell Biology 2022Quote: ... RNAi was transfected into primary astrocytes at 80-90% confluence using Lipofectamine RNAiMax (Invitrogen by Thermo Fisher, Cat. No. 56531). Knockdown efficiency was confirmed with qPCR as well as western blotting ...
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bioRxiv - Biochemistry 2022Quote: ... the cell suspension typically contains >90% cardiomyocytes and were seeded in black optical bottom 96-well plates (Thermo Scientific, 165305) coated with human plasma fibronectin + 0.1% gelatin in DMEM low glucose + 7% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... Native complexes were size separated for 30 min at 150V with Dark Blue cathode buffer and 90 min at 250V with Light Blue cathode buffer (Invitrogen). Finally ...
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bioRxiv - Physiology 2021Quote: ... Cells were transfected when at a confluence about 80-90% using Lipofectamine® 3000 Transfection Kit (Thermal Fisher Scientific, 2232162) based on manufacturer’s instruction.
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were further diluted 1:10 (10 μL into 90 μL) in phosphate-buffered saline (PBS) (Gibco,18912-014) containing 2 mg/mL kanamycin to halt protein translation ...
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bioRxiv - Microbiology 2021Quote: ... the plugs were covered with dilute compost extract in 90 mm triple-vented petri dishes (five per plate; Thermo Scientific) and were placed under constant light conditions at 20 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting solution was added dropwise to a T75 flask of 90% confluent HEK293T cells grown in 10% FBS in DMEM media (Gibco) and gently mixed by tilting ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... this complex was incubated for 90 minutes with 50 μl horseradish peroxidase-conjugated antibody (anti rabbit, IgG, Fisher Scientific; NA934) diluted in 50% PBS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a 2 hour room temperature incubation in 10% NGS/90% antibody buffer containing the following fluorescently-conjugated secondary antibodies: Goat anti-mouse Alexa 488 (1:500; Invitrogen) and goat anti-rabbit Alexa 594 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cell Biology 2021Quote: ... The RNP mixture containing 1.8 μl of sgRNA with 3 μl (90 pmol) Truecut Cas9 v2 (Thermo Fisher; catalog A36498) in a total volume of 5 μl was incubated at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... the pellet was resuspended in 90 μl of Secondary antibody solution (Blocking buffer + 1:500 Goat anti-rabbit IgG AlexaFluor 647 antibody [#A27040, Invitrogen]) and the samples were incubated at room temperature for 45 min in the dark ...
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bioRxiv - Biophysics 2023Quote: ... Sorted cells were allowed to grow to 90% confluency and plated at 0.7 × 106 cells in FluroBrite DMEM (Gibco A1896701) supplemented with 10% FBS per well in a 6-well TC plate ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific]; 5 M NaCl [#01094764, ThermoFisher Scientific]; 90 mM KCl [#01276444, ThermoFisher Scientific] ...
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bioRxiv - Cancer Biology 2023Quote: ... a 6-well plate of cells at approximately 80-90% confluency was harvested by treatment with trypsin without EDTA (Gibco). The staining was performed by the use of an Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme) ...
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bioRxiv - Bioengineering 2023Quote: The normal human fibroblast cell line IMR-90 (ATCC CCL-186) expressing the fluorescent protein GFP was cultured in Dulbecco’s Modified Eagle Medium (Gibco, USA) containing 4.5 g/L D-glucose ...
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bioRxiv - Microbiology 2023Quote: ... The MCF7 (ER+, PR +/-, HER2 0, Ki-67 90%) (Subik et al., 2010) cell line was maintained in Minimum Essential Media (Gibco) supplemented with 10% fetal bovine serum (Atlanta Biologics) ...
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bioRxiv - Microbiology 2023Quote: ... the cells were pre-treated during 90 mintes with an anti-bleaching media (DMEM without phenol red, Ref 31053023, Gibco) containing 1% Penicillin-Streptomycin ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were cultured until ∼90 % conflueny in a cell culture incubator (Heracell™ 150i CO2 Incubator, 50116048, Thermo Scientific™) under a 5 % CO2 atmosphere at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The upper 90% of the supernatant was extracted and dialysed using Slide-A-Lyzer™ cassettes (Thermo Scientific, Cat. 66330) with a 2 kDa molecular weight cut off ...
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bioRxiv - Molecular Biology 2024Quote: Retroviral particles were produced by transfecting pLPC plasmids into low passage Phoenix cells (ATCC) at 90% confluence using Lipofectamine3000 (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... they were exposed for 90 min to 1% BSA in PBS containing a secondary antibody (Alexa Fluor 488 anti-rabbit IgG, Invitrogen) at RT.
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bioRxiv - Microbiology 2024Quote: Digested peptides were analyzed with 90-minute gradients in C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000,3500 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive Orbitrap HF ...
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bioRxiv - Microbiology 2023Quote: ... aureus strains (Cowan I and LE2332) at MOI 1 for 90 minutes ROS and NO production by monocytes was quantified by using CellROX Green (ThermoFisher) and DAF-FAM Diacetate (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice with 90 µL of PBS before incubated with a secondary rabbit anti-goat antibody coupled to Alexa647 (Invitrogen) at 1:100 dilution ...
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bioRxiv - Genetics 2023Quote: ... and the cells were counted and centrifuged before being resuspended in 90% M2 medium and 10% FBS (Cat#A31606-01 Gibco). From day 16 to day 30 ...
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bioRxiv - Microbiology 2022Quote: ... strain F HSV-1 stocks were prepared by infecting African green monkey kidney (vero) cells at 80–90% confluency in serum-free VP-SFM (Gibco). The virus was harvested 2 d after infection ...
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bioRxiv - Biophysics 2022Quote: 3T3 cells were cultured in plastic cell culture flasks in culture media (90 ml DMEM with glutamine (Gibco 22320-022), 10 ml Fetal Bovine Serum (Gibco 25300054) ...