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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... 50 °C for 30 s and 72 °C for 90 s) with the DreamTaq Hot Start Greeen PCR Master Mix (Thermofisher scientific, Waltham, MA, US) in a final volume of 50 μl ...
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bioRxiv - Physiology 2021Quote: ... Neurobiotin brains were dehydrated and rehydrated in an alcohol series (50 %, 70 %, 90 %, 100 %) and propylene oxide and next incubated in Oregon Green-avidin (Oregon green® 488 conjugate, Molecular probes, Oregon, US) with 0.2 % Triton X and 1 % BSA overnight at 4 ° C (58) ...
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bioRxiv - Immunology 2021Quote: ... ventral and dorsal sheets of the ear were separated from the cartilage and incubated for 90 min at 37 °C in RPMI 1640 (Invitrogen, Grand Island, NY, USA) containing 0.01% DNAse (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... dehydrated through a graded ethanol series (50, 70, 90 and 100%) and washed in xylene before being mounted with coverslips on DPX mounting medium (ThermoFisher Scientific, Waltham, MA, USA). Immunostaining was not observed in negative control tests without the primary antibodies or with primary antibodies that had been pre-adsorbed with the antigen peptide at a concentration of 20 µM (data not shown).
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bioRxiv - Genetics 2023Quote: ... The nematodes were then digested by incubating the PCR strips at 65°C for 90 minutes followed by inactivation of Proteinase K at 95°C for 15 minutes in a Thermal Cycler (Applied Biosystems ProFlex PCR System).
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bioRxiv - Neuroscience 2023Quote: ... based 4-20 % polyacrylamide (Rotiphorese Gel 30; Carl Roth) gradient gels and run (80-120 V; 90 min) in NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific; Cat#NP000102) using Mini-PROTEAN Tetra Vertical Electrophoresis Cells (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... chopped lung tissue was incubated at 37°C for 90 minutes (mins) in RPMI with 5% fetal bovine serum (FBS) and 150 U/ml collagenase (Life Technologies, Rockville, MD, USA). Nonadherent cells were enriched on Percoll density gradients (44/67% ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 90 min at room temperature with secondary antibodies diluted in PBS/1% BSA: Alexa488 goat anti-rabbit (Life Technologies # A11070; 1/1200) or Alexa594 goat anti-mouse (Life Technologies # A11020 ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293/GC-A+/AT2+ cells were cultured in 60 mm dish to reach 90-100% confluency before being harvested in RIPA buffer (Thermo Fisher Scientific, Waltham, MA) with protease inhibitors ...
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bioRxiv - Cell Biology 2024Quote: ... and 90 minutes by radioimmunoprecipitation assay buffer (RIPA) with Halt™ protease inhibitor and Halt™ phosphatase inhibitor cocktails (all from Thermo Fisher Scientific). Protein concentrations were determined by Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... cell line GRANTA-519 waskept in culture in RPMI 1640 medium supplemented with 10% heat-inactivated FBS 2 mM L-glutamine and 1% penicillin/streptomycin antibiotics (Gibco®, Thermo Fisher Scientific, Carlsbad, CA, USA). For cultivating the lymphoblastoid cell line (LCL ...
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bioRxiv - Cell Biology 2020Quote: ... LC separation was performed using an Acclaim PepMap RSLC C18 capillary column (75 μm × 25 cm; 2 μm particles, 100 Å) (Thermo fisher Scientific, San Jose, CA). Gradient elution was performed using an Ultimate 3000 nanoLC system (Thermo fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... / 3% ACN in water and then separated through a PepMap C18 analytical column (75 μM X 50 cm, 2 μM, 100 Å, Thermo Fisher Scientific, San Jose, CA) at a flow rate of 300 nL/min ...
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Molecular Biology 2021Quote: ... and FURA 2-AM (Fura-2-acetoxymethyl ester) from Invitrogen, CA ...
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bioRxiv - Cell Biology 2021Quote: ... A fluorescent derivative of 2-deoxyglucose (2-NBDG, Invitrogen, N13195) was added at a final concentration of 20 µM for a further 30 min incubation at 37 °C ...
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The structure of insulin granule core determines secretory capacity being reduced in type-2 diabetesbioRxiv - Physiology 2022Quote: ... Nucleobindin 2 and siRNA control #2 were purchased from ThermoFisher Scientific ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... 2 mM L-Gln and 50 μM 2-mercaptoethanol (Invitrogen).
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bioRxiv - Genetics 2022Quote: ... 2% B-27 and 1% N-2 supplements (Life Technologies) and supplements 20ng/ml BDNF ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were loaded with 2 μM Fura-2/AM (Invitrogen). Ratiometric Ca2+ imaging was performed at 340 and 380 nm in 0 or 2 mM Ca2+ Ringer’s solution using an IX81 microscope (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME, Thermo Fisher Scientific)) supplemented with 4 ng/ml bFGF ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM L-glutamine and 2% penicillin/streptomycin (ThermoFisher Scientific). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... with 2 pulses of 950V lasting 2 ms by Invitrogen Neon Transfection system (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME, Thermo Fisher Scientific), supplemented with 100 ng/mL Activin A (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2024Quote: ... A 2 × 2 cm piece of Parafilm M (Fisher Scientific) was carefully placed onto the tissue so that the gene panel mix was evenly spread across the tissue and no bubbles were introduced between the tissue and the film ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... containing 2% of 2-mercaptoethanol (Thermo scientific Cat. No: 125470100) and heated at 70°C for 10 mins before freezing them at – 80°C ...
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bioRxiv - Microbiology 2024Quote: ... IL-2 was measured by human IL-2 ELISA (Invitrogen) using the manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 2-Nitrobenz Oxadiazol Amino Deoxyglucose (2-NBDG, Invitrogen, Cat# N13195) for 30 minutes (figure 4(a)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μg mL−1 puromycin (58-58-2, ≥98%, Invitrogen) and 100 μg mL−1 hygromycin (31282-04-9 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with 2 μM Fura-2 (F1221, Invitrogen) at 37℃ for 30 min dispersed in Margo solution with 0.2‰ w/v Pluronic F-127 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µL FastAP Thermosensitive Alkaline Phosphatase (2 U) (Thermo Scientific) was added and the solution was incubated at 37°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 2 mL sample was preserved in 2 mL RNAlater (Invitrogen), stored at 4°C until all national samples were collected (usually within a few days) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 % penicillin-streptomycin) supplemented with 2% N-2 supplement (Gibco), 1% B-27 supplement serum free (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: Biosafety Level 2 (BSL-2) laminar flow hood (Thermo Fisher scientific #1300 Series A2 or equivalent)
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bioRxiv - Biophysics 2020Quote: ... respectively (Life Technologies, Carlsbad, CA).
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bioRxiv - Cell Biology 2020Quote: ... or DAPI (Invitrogen, CA, USA) was used as a nuclear marker ...
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bioRxiv - Genetics 2021Quote: ... (Affymetrix, Santa Clara, CA, USA). Further details about imputation ...
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bioRxiv - Genomics 2020Quote: ... respectively (Life Technologies, Carlsbad, CA). RNA quality was evaluated using the Agilent 2200 TapeStation and RNA ScreenTape (Santa Clara ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobasal(-A) (Invitrogen, Carlsbad, CA), B27(-A ...
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bioRxiv - Neuroscience 2021Quote: ... B27(-A) (Invitrogen, Carlsbad, CA), heparin (2ng/mL) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNaseA (Affymetrix, Santa Clara, CA) was diluted 1:1000 in 1xPXL ...
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bioRxiv - Bioengineering 2020Quote: ... Cell culture medium and reagents were obtained from Gibco (Thermo Fisher Scientific, Valencia, CA). Cells were propagated to a maximum of 20 passages.
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bioRxiv - Microbiology 2021Quote: ... pH 7.4 (Invitrogen, Carlsbad, CA). The flasks were swirled until the lipids were in suspension ...
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bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 3000 (Invitrogen, CA, USA) was mixed with 2.5 μg of plasmids for EGFP-FL CEPB3 (1-698aa) ...
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bioRxiv - Cancer Biology 2020Quote: ... DMEM (Life Technologies, Carlsbad, CA) supplemented with 10% FetalPlex (Gemini Bio-Products ...
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bioRxiv - Bioengineering 2020Quote: ... Lysotracker dye (Invitrogen, Carlsbad, CA) was incubated with cells for 30 min ...