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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HK-2 was seeded in 6 well plates (1 × 106 cells/well) or 96 well plates (1 × 105 cells/well) and cultured in 90% RPMI1640 medium (Gibco, USA) supplemented with 100 U/mL penicillin ...
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bioRxiv - Biochemistry 2021Quote: Cells were transfected at 90% confluency in 60 mm dishes and using 5 µg DNA and lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Size exclusion fractions enriched in crosslinked peptides were combined into two mass spectrometry samples and each one was acquired over a 90 minute UPLC gradient acquiring sequential HCD and EThcD product ion spectra on a Fusion Lumos mass spectrometer (Thermo Scientific). Peaklists were generated using PAVA for each dissociation method and searched with Protein Prospector 6.3.23 ...
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bioRxiv - Neuroscience 2021Quote: ... After drying the slides were dehydrated using a progression of alcohol (30%, 60%, 90%, 100%, 100% ethanol) followed by Citrasolv (Fisher Scientific) before coverslipping with Permount (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... Peptides were eluted along a 90 min gradient from 6 to 40% Buffer B (see StageTip purification) with the EASY□nLC 1,200 system (Thermo Fisher Scientific). Measurement was done on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01, in 10□×□PBS pH 7.2 (Gibco, #70013032). The final volume was topped up with DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were multiplexed and analyzed by LC-MS/MS using a 90-minute gradient on Orbitrap Eclipse (Thermo Fisher, Waltham, USA) with a method using CID-MSA for the identification of MS2 and a real-time search algorithm to quantify reporters in MS3 ...
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bioRxiv - Microbiology 2023Quote: ... Growth medium on HEK293T cells at 90% confluency in 6 well plates was replaced with Opti-MEM reduced serum medium (Thermo Fisher) and cells in each well were transfected with 250ng each of 8 pDUAL reverse genetics plasmids ...
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bioRxiv - Microbiology 2024Quote: ... The viral media overlayed on the sorbitol cushion was applied to a Sorvall WX-90 ultracentrifuge and SureSpin 630 rotor (Thermo Scientific) at 20,000 rpm for 1.5 hours at 18°C ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were then incubated for 90 min at room temperature with secondary antibodies (1:500, Alexa Fluor 568, Alexa Fluor 488, Life Technologies). After three rinses ...
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bioRxiv - Physiology 2022Quote: Production of NO was measured in HUVEC exposed to BPS for 30 or 90 min by loading with a DAF-FM Diacetate probe (Invitrogen, D23844) for 45 min at 37°C and quantifying on a SpectraMax M2 plate reader or flow cytometer ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were eluted from the column using a linear acetonitrile gradient from 2 to 30% acetonitrile over 90 minutes followed by high and low organic washes for another 20 minutes into an LTQ XL mass spectrometer (Thermo Scientific) via a nanospray source with the spray voltage set to 1.8kV and the ion transfer capillary set at 180°C ...
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bioRxiv - Bioengineering 2024Quote: ... water and then dehydrated through graded ethanol series (in %: 50, 70, 80, 90, 95 and 100) (Thermo-Fisher Scientific, Waltham, MA) before proceeding with Critical Point Dryer (CPD ...
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bioRxiv - Genomics 2024Quote: ... to a final volume of 200 μL and then incubated 90 min at 37°C with 6 μL of RNase cocktail (Ambion, AM2286), followed by 150 minutes at 55°C with Proteinase K (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... cell concentration and viability (80-90%, see fig. S1B) of each cell suspension were determined using 0.4% trypan blue (Gibco™: 15250061). Cells were washed and resuspended in 1x PBS (calcium and magnesium free ...
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bioRxiv - Biochemistry 2024Quote: ... reaction were applied to grids for 60 seconds at room temperature and 90% humidity in a Vitrobot Mark IV (Thermo Fisher). Grids were double-side plotted with force 0 for 5 seconds and immediately plunge frozen in liquid ethane ...
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bioRxiv - Molecular Biology 2023Quote: ... or 90 pmol of SBP-tagged eIF4A1 protein was incubated with 30 μl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific), which had been preequilibrated with equilibration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then blocked with a 5% (v/v) FBS solution and incubated for 90 min in a 1:200 phalloidin–Alexa Fluor 594 (Life Technologies) diluted in a 1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated with a 90 μl primary antibody solution containing mouse anti-cTnT primary andibody (1:100; Cat. #: MA5-12960, Invitrogen, USA) and rabbit anti-IRX4 primary antibody (1:100 ...
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bioRxiv - Bioengineering 2024Quote: ... at concentrations ranging from 1.6-16 mM for 90 minutes before being detached from the surface by treatment with 0.25 % trypsin-EDTA (Thermo Fisher Scientific). The detached cells were then centrifuged at 350 x g for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... were formed by incubating 90 pmol sgRNA and 10 pmol Cas9-2NLS in Buffer R (Component of Neon Transfection System Kit; Thermo Scientific) at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cells at 80∼90% confluence were transfected with 200 ng of pCAGGS derivates using Lipofectamine® 2000 (11668027, Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Human fetal lung fibroblast (Institute for Medical Research-90, IMR90, ATCC) cells were cultured in Minimum essential medium (MEM, 1×) (Invitrogen, USA) containing 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... at 300 nL/min over 90 minutes and eluted through a nanospray interface into a Q-Exactive Orbitrap mass spectrometer (Thermofisher Scientific). Qualitative and label-free quantification was performed using Proteome Discoverer 2.3 37 ...
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bioRxiv - Cancer Biology 2022Quote: ... was dissolved in a diacetyl-L-tartaric anhydride(DATAN) solution (90 μl, 50 mG/ml in freshly mixed 80% acetonitrile/20% acetic acid, DATAN, Acros Organics). The solution was sonicated ...
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bioRxiv - Immunology 2022Quote: ... The media was then removed from each well and replaced with 90 μL fresh media and 10 μL PrestoBlue reagent (ThermoFisher Scientific) and incubated at 37°C and 5% CO2 for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Phage DNA integrity was checked using agarose gel electrophoresis (1 % agarose gel in 1× TAE buffer; 70 V, 90 min) against a GeneRuler 1 kb ladder (ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: Cell suspensions containing formaldehyde-fixed AnTat1.1E 90–13 GFP::PAD1utr parasites were stained with a PBS solution containing 0.005 mg/mL Hoechst 33342 (Thermo Fisher Scientific) for 20 min at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... standard curve analysis was carried out to ensure that the amplification efficiency of PCR for each primer pair was within the prescribed range of 90-110% (Applied Biosystems). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2023Quote: NPK-C1 cells at 70-90% confluence or MC38 cells at 50-75% confluence were harvested with 0.05% trypsin (Gibco, Gaithersburg, MD), washed with PBS ...
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bioRxiv - Genetics 2023Quote: ... primary myoblasts were labelled for 90 minutes and processed with EdU Click-iT™ EdU Cell Proliferation Kit (C10337, ThermoFisher, GmbH). Percentage of EdU+ nuclei was determined after normalizing the EdU+ nuclei to DAPI stained nuclei.
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bioRxiv - Neuroscience 2024Quote: ... the slides were dehydrated in a series of ethanol (30%, 60%, 90%, 95%, 100%, 100%) and cleaned with Citrisolv (Fisher Scientific). We then cover-slipped slides with Permount (Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... a half-media change was conducted and the Lipofectamine 2000 protocol for transfection was followed using the materials mentioned along with 90 µL of Lipofectamine 2000 (Invitrogen, 11668019). Virus was prepared and stored as detailed previously.
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bioRxiv - Cell Biology 2024Quote: IMR-90 human fibroblast was purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Gibco, 12800-017) with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown in 150 mm dishes to ∼90% confluence then washed with sterile PBS and detached (Gibco TrypLE Express #LTS12604021). Cells used for all experiments were ≤9 passages from stock and tested negative for mycoplasma contamination (Agilent MycoSensor QPCR Assay Kit ...
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bioRxiv - Bioengineering 2024Quote: ... a half-media change was conducted and the Lipofectamine 3000 protocol for transfection was followed using the materials mentioned along with 90 µL of Lipofectamine 3000 and 30 µL P3000 (Invitrogen, L3000001). Supernatants were collected 24 ...
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bioRxiv - Microbiology 2024Quote: ... to let the bacteria and aggregates adsorb before removing the liquid and replacing it with 90 µL tyramide reagent containing tyramide conjugated with Alexa Fluor 488 (1:100) (Invitrogen, B40953), 2 mM ATP (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... and 5 units/mL DNAse (MilliporeSigma) for 90 min at 37°C and then filtered through a 70 μm cell strainer (Thermo Fisher) and treated with ACK lysing buffer (Worthington Biochemical) ...
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bioRxiv - Bioengineering 2024Quote: ... 80-90 % of cell cultures were split into a ratio of 1/3 to 1/6 using Tryple Express Enzyme (Gibco, 12604021) and Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... 45-90 nl of fluorophore-conjugated (AlexaFluor 488, 555 or 647) cholera toxin subunit B (CTB; C34775, C34776 or C34778, ThermoFisher Scientific) or 45-90 nl of red Retrobeads or Retrobeads IX (Lumafluor ...
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bioRxiv - Cancer Biology 2024Quote: ... MDA-MB-231 cells 70-90% confluent were washed two times with cold PBS and 600 μL TRIzol reagent (Invitrogen, 15596018) was used to collect the cells ...
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bioRxiv - Biochemistry 2024Quote: ... 6-well plates of sub confluent 70-90% CAL51 cells were incubated for 6h at 37°C in fresh medium DMEM high Glucose (Gibco, 11965) + 10% FBS (PAN Biotech ...
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bioRxiv - Biochemistry 2024Quote: ... 12-well plates of sub confluent 70-90% CAL51 cells were incubated for 24h at 37°C in fresh medium DMEM high Glucose (Gibco, 11965) + 10% FBS (PAN Biotech ...
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bioRxiv - Genetics 2024Quote: Concentrated RENDER-CRISPRoff (v3) were denatured at 90 °C for 5 min in reducing Laemmli SDS sample buffer (Thermo Fisher Scientific). Denatured proteins were separated on a NuPAGE 3-8% Tris-Acetate Gel (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... filtered through a 40 μm filter to achieve > 90% single cells and evaluated by Trypan Blue Solution (Thermo Fisher Scientific, 15250061) to confirm > 90% cell viability ...
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bioRxiv - Cell Biology 2024Quote: ... The grids were then blotted for 7.5 s at ∼90 % humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: Viably frozen healthy BM and PDX samples (Patient ID: CR2, CR4, CR6, Neg2, Neg5, Neg11) were thawed in 90% RPMI medium (ThermoFisher Scientific) with 10% FCS ...
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bioRxiv - Cell Biology 2024Quote: ... and after 24h the E8 media was removed and 90 μL of E8 Flex media and 10 μL of PrestoBlue HS reagent (Invitrogen, USA) were added ...
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bioRxiv - Cell Biology 2024Quote: Human iPSCs were grown in 6-cm cell culture dish until reached 80-90% confluence and were treated with 0.1 ug/mL Colcemid (Cat. #15212012, KaryoMAX Colcemid, Gibco, Thermo Fisher Scientific) and 0.5 uM Wee1 inhibitor (PD0166285 ...