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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Microarray transcriptomic data from UII-hFGFR3-S249C and BBN mice was combined with transcriptomic array data from human bladder tumors (CIT; Affymetrix Exon 1.0 ST; 96 MIBC and 99 NMIBC). Batch effects due to data combination were corrected using the surrogate variable analysis R package ...
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Can Intestinal Absorption of Dietary Protein Be Improved Through Early Exposure to Plant-Based Diet?bioRxiv - Zoology 2020Quote: ... 2 µg of RNA from each sample was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) to obtain a 20 µl cDNA solution ...
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bioRxiv - Microbiology 2021Quote: ... Western blots were probed with α- CP or α-RRV E2 primary antibodies at a 1:5000 dilution in 2% milk in TBST followed by AlexaFluorTM750 goat α-rabbit secondary antibody (Invitrogen A21039, Carlsbad, CA) at a 1:20,000 dilution in 2% milk in TBST ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Biophysics 2021Quote: ... The filtered lysate was diluted 1:2 in purification buffer UA (8 M Urea, 50 mM sodium acetate [Acros Organics, Carlsbad, CA], pH 4). The protein was then purified using a cation exchange HiPrep SP HP 16/10 (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... After that, 2 µl of RT mix (1x SuperScript II first-strand buffer, 18 U/µl SuperScript II reverse transcriptase (Life Technologies, Carlsbad, CA, USA), 3.6 µM C1-P1-RNA-TSO (5’-AAU GAU ACG GCG ACC ACC GAU NNN NNG GG -3’) ...
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bioRxiv - Microbiology 2020Quote: ... The eluted peptides were electrosprayed at 2 kV into a dual linear quadrupole ion trap Orbitrap Velos Pro mass spectrometer (MS) (Thermo Fisher, San Jose, CA). The MS was set to switch between a full scan (400 < m/z > 2000 ...
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bioRxiv - Systems Biology 2022Quote: ... 3 μm particle size) connected to an Acclaim PepMapTM RSLC C18 analytical column (50 μm x 15 cm, 2 μm particle size) (Thermo Scientific, San Jose, CA). Of each sample ...
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bioRxiv - Evolutionary Biology 2022Quote: A 2-μg aliquot of the peptide mixture was analyzed using a LTQ-Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse and/or goat anti-rabbit AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500) secondary antibodies ...
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bioRxiv - Physiology 2020Quote: ... such as trypsin and chymotrypsin were determined in pancreatic tissue homogenates using fluorescent substrates as previously reported (34): Trypsin substrate (R110-(CBZ-Ile-Pro-Arg)2 was purchased from Life Technologies (Carlsbad, CA, USA); Chymotrypsin (Suc-AAPF-AMC ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and incubated for 2 hours at RT with Alexa Fluor 488 donkey anti-rabbit (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 555 donkey antimouse (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... from an Acclaim PepMapTM RSLC C18 nano Viper analytical column (2 mm, 100 Å, 75-μm ID × 50 cm, Thermo Scientific, San Jose, CA) using a gradient of 5-25% solvent B (80/20 acetonitrile/water ...
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bioRxiv - Microbiology 2020Quote: ... from an Acclaim PepMap™ RSLC C18 nano Viper analytical column (2 μM, 100Å, 75-μm ID × 50 cm, Thermo Scientific, San Jose, CA) using a gradient of 5-25% solvent B (80/20 ACN/water ...
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bioRxiv - Immunology 2023Quote: ... 25-40% acetonitrile for 20 min followed by a 90% acetonitrile wash for 10 min and a further 30 min re-equilibration with 2% acetonitrile) and directly introduced into Q Exactive HF mass spectrometer (Thermo Scientific, San Jose CA). MS1 scans were acquired at 60K resolution (AGC target=3e6 ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA was synthesized from 1–2 μg of DNase-treated total RNA using SuperScript™ IV Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) and a mixture of anchored oligo(dT)18 and random octamer primers (Genomics Core Facility ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then incubated for 2 h at 4℃ with Alexa fluor 488-conjugated secondary antibody (1:200, Invitrogen, #A21206, Carlsbad, CA, USA) or DAPI (1:30000 ...
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bioRxiv - Molecular Biology 2022Quote: ... ACE2-expressing HEK293T cells were seeded to 24-well plates and transfected with 2 μg of each mRNAs/well using Lipofectamine™ MessengerMAX™ Transfection Reagent (Invitrogen, CA, USA). After 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Microbiology 2023Quote: ... Bodies and legs/wings were placed into 2-mL tubes containing 500 µL PBS and a 5-mm glass bead (Thermo Fisher Scientific, Emeryville, CA), then homogenized at 30 hertz for 5 minutes in a TissueLyser (Retsch ...
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bioRxiv - Neuroscience 2022Quote: ... slides were rinsed with 0.025% Triton X-100/1xTBS 2 × 5 min and incubated in goat anti-rabbit AlexaFlour488 secondary antibody (1:1000, Life Technologies, #A27034, Carlsbad, CA) at RT for at least 2 h followed by 4’,6-diamidino-2-phenylindol (DAPI ...
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bioRxiv - Pathology 2024Quote: ... and predesigned TaqMan-based qPCR assays for target and housekeeping genes (Table 2) and run on the 7500 Fast Real-Time PCR System and QuantStudio 3 thermocyclers (Applied Biosystems; Waltham, CA, USA). Relative expression values were normalized by the expression of housekeeping genes using the 2-ΔΔCt method ...
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bioRxiv - Immunology 2024Quote: ... slides were rinsed with 0.025% triton X-100 in TBS (2 × 5 min) and incubated in goat anti-rabbit AlexaFluor488 secondary antibody (1:1000, Life Technologies, #A27034, Carlsbad, CA) or goat anti-rat AlexaFluor594 secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissues were washed with PBTBN three times and incubated for 2 h with Alexa-Fluor 647 goat anti- mouse or anti-rat IgG (Molecular Probes, Carlsbad, CA, USA) diluted 1:400 in PBTBN ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK-293 epithelial cells (CRL-1573) and HeLa cells (CCL-2) purchased from the ATCC were maintained in DMEM (Life Technologies Inc, Carlsbad, CA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... and ADAM9 siRNA-2 (AD9-2) (Ambion 4390826). Antibodies used in this study include rabbit anti-ADAM9 (CST 4151 ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were blocked with blocking solution (CAS Block) (Invitrogen, San Diego, CA), followed by incubation with the primary antibody overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were blocked and permeabilized in CAS-T (CAS-Block (Thermo Scientific) with .2% Triton-X ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked at RT in PBST-CAS (CAS blocking; ThermoFisher #00-8120) for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... CG (Applied Biosystems, CA) and run on 7500 Fast Dx Real-Time PCR Instrument with SDS 1.4 software ...
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bioRxiv - Genomics 2020Quote: ... CG (Applied Biosystems, CA) and run on 7500 Fast Dx Real-Time PCR Instrument with SDS 1.4 software ...
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bioRxiv - Bioengineering 2021Quote: ... from Applied Biosystems (Life Technologies, Carlsbad, CA). The Arabidopsis eEF-1 α gene (Assay ID ...
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bioRxiv - Cell Biology 2021Quote: ... (Affymetrix, Santa Clara, CA).
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bioRxiv - Genomics 2021Quote: ... sense ssDNA suitable for labeling was generated from total RNA with the GeneChip WT Plus Reagent Kit from Affymetrix (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... B27 (Invitrogen, Carlsbad CA), glutamine (CCF media core) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lipofectamine 3000 was purchased from Invitrogen (Invitrogen, Camarillo, CA, USA). Page Ruler Plus Prestained protein ladder was purchased from Thermo Fischer Scientific (Rockford ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... coli (Invitrogen, Carlsbad, CA) cells ...
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bioRxiv - Cell Biology 2022Quote: ... GFP (Invitrogen, Carlsbad, CA), AIMP1 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... (Gibco™, Carlsbad, CA) as a suspension.
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bioRxiv - Immunology 2020Quote: ... 20,000 (Invitrogen, Carlsbad, CA)] ...
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bioRxiv - Cancer Biology 2022Quote: ... and other culture materials were from Invitrogen (Life Technologies, Carlsbad, CA). Antibodies against PKD2 ...