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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: S2 cells (cat# CRL-1963, ATCC) were maintained at 27 °C in 90% Schneider’s Drosophila Medium (Cat# 21720024, ThermoFisher) suppled with 10% heat-inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... MS was removed and 90 μL of MS was added together with ammonium persulfate (APS, 17874, Thermo Fisher Scientific) and tetramethylethylenediamine (TEMED ...
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bioRxiv - Microbiology 2024Quote: ... was pre-run at 90 V for 5 minutes with 0.5X TBE buffer (prepared from 5X TBE buffer (Thermofisher)) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by secondary antibody incubation with species-specific Alexafluor IgG secondary antibodies (90 min at RT, Invitrogen; 1:1000). Tissues were washed again in 0.1% PBST (4 x 15 min) ...
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bioRxiv - Microbiology 2024Quote: ... sg.ORF7a) and genomic RNA (NSP3 region; g.NSP3) (90) on a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). Relative mRNA expression was normalized to the levels of GAPDH and expressed relative to the values for control cells using the ΔΔCt method.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Subcultivation of monolayers was performed at 70–90% confluence by detachment using TrypLE (Gibco™ Ref 12604013, Paisley, UK). For experiments ...
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bioRxiv - Neuroscience 2024Quote: ... supernatant centrifugation at 100.000g for 90 mins at 4 °C in a fixed-angle rotor (TH865, Thermo Fisher Scientific), followed by pellet wash with 1x PBS and centrifugation at 100.000g for 90 mins at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... B6 and MRL/MpJ cells were seeded at 90% confluency on NuncTM EasYFlaskTM culture flasks (#159910; Thermo Fisher Scientific), rinsed once with 1X DPBS (#21-030-CV ...
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bioRxiv - Microbiology 2024Quote: ... incubated for 90 min in infection medium with 1 µM BODIPY-FL-C12-sphingomyelin (Thermo Fisher Cat. No. D7711), 10 µM BODIPY-FL-C12-ceramide (Santa Cruz ...
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bioRxiv - Biophysics 2024Quote: ... A total of 1.1 mg/L of DNA was used at a 2:1 ratio of hNaV1.8 to β4 and was mixed into 90 mL of Opti-MEM Reduced Serum Medium (Gibco). A total of 10 mL (1 mg/mL in PBS ...
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bioRxiv - Genetics 2024Quote: ... after which 10 µL was mixed with 90 µL of ATP Determination reaction mixture (Cat. # A22066, Thermo-Fisher Scientific) and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... ticks from each pool were put into a 2 mL centrifuge tube containing 1 mL of Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) prior to being homogenized in a frozen grinder (Jingxin ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... We converted 2 μg of RNA to cDNA with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Quantitative PCR on Quant Studio 12K Flex (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alexa Fluor 488–conjugated phalloidin (#A12379) and 4’,6-diamidino-2-phenylindole (DAPI) (#1306) were obtained from Invitrogen (Carlsbad, CA, USA). IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Microbiology 2022Quote: ... The Hela cell line was provided by HeLa CRM-CCL-2™ and maintained at 37°C with 5% CO2 in Dulbecco’s high glucose modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μg of total RNA was reverse transcribed with oligo-dT primers and SuperScript III reverse transcriptase (Life Technologies, Carlsbad, CA). Quantitative PCR reactions were run using Power SYBR PCR mix (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: Phage DNA was isolated by treating 2 mL of phage solution with 1 U/μL DNase I (Invitrogen, Carlsbad, CA, USA) to remove free DNA ...
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bioRxiv - Neuroscience 2024Quote: ... substituted with 10% fetal calf serum (FCS; Capricorn Scientific, Palo Alto, CA, USA) and 2 mM L-glutamine (Invitrogen, Carlsbad, USA), 50 U/ml penicillin/streptomycin (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed on 4-μm thick sections containing cross-sections of the entire ileal length and were imaged at 10X using an EVOS FL Auto 2 Cell Imaging System (Invitrogen, CA). H&E stained sections from each animal were graded for intestinal damage according to a histological NEC damage grade severity scoring system ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Carlsbad, CA, USA). Cells were viewed using an Olympus IX81 or Olympus BX53 epifluorescence microscope ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 µg RNA samples were reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). Additional file 1 presents the SYBR Green assay primers ...
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bioRxiv - Cancer Biology 2024Quote: ... A549 and/or H1299 cells were cotransfected with 2 ug of NPRL2 plasmid DNA (pKGB2-NPRL2) and 1 ug of pcDNA3.1 vector (Invitrogen, Carlsbad, CA) containing a neomycin-resistant gene ...
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bioRxiv - Bioengineering 2024Quote: ... the embryos were incubated in secondary antibody solution (1 mL CAS-Block containing 2 μL Alexa Fluor 488 (Invitrogen, A-11006), 2 μL Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... 2) designed and tested in a genomic cleavage detection (GCD) assay in Neuro 2A murine cells by Thermo Fisher (Carlsbad, CA). Based on the results of the validation ...
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bioRxiv - Genomics 2021Quote: ... with protease inhibitor cocktail (Leupeptin, Carl Roth, CN33.4; Pepstatin A, Carl Roth, 2936.3; PMSF, Carl Roth, 6367.3; Benzamidine hydrochloride, Acros Organics, 105245000, resuspended in ethanol) and 250 U/μL benzonase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: Total RNA of cells was extracted after treatment of cells with sertraline hydrochloride for 72 h using TRIzol reagent (Cat no. 15596-018, Ambion, Life Technologies, USA). Complementary DNA (cDNA ...
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bioRxiv - Immunology 2020Quote: ... was added and incubation continued 24 hours before changing the media with Screening media (DMEM+10% FBS containing 10 μg/ml Blasticidin S hydrochloride (Invitrogen, cat#21001)) ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 was isolated from 0.35 mg of lysate by 90 min incubation at 4°C with 20 µl Protein-G Dynabeads (ThermoFisher, 10004D) and 2 µg of each of three anti-CDC20 mAbs ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transferred to 90 mm culture dishes to undergo negative selection with 50 µg/ml Hygromycin B (Gibco) until all non-transfected cells were dead ...
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bioRxiv - Neuroscience 2021Quote: ... BV2 cells were seeded in 24 well-plates at 70-90% confluence 24 hours prior to the experiment in high glucose DMEM (Gibco) supplemented with 10% FBS without antibiotics ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were incubated for 90 minutes with Alexa Fluor 488-conjugated donkey-anti-rabbit secondary antibody (1:300; Invitrogen; A21206) and Cyanine Cy™3-conjugated donkey-anti-rat secondary antibody (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... and then the cells were incubated for 0-90 minutes at 37 °C with 100 µl of pHrodo Red E.coli (ThermoFisher, P35361). The BMDCs were washed three times with PBS and resuspended in FACS buffer ...
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bioRxiv - Immunology 2022Quote: Total or individual CD8+ or CD4+ T cells were purified from PBMC suspensions from our PLWH and HIV-1 negative control study cohorts by negative immunomagnetic selection (purity >90%) using the Untouched Human T cell and CD4 T cell Dynabeads Kits (Invitrogen) and the MojoSort Human CD8 T cell (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum and 2 mM L-glutamine at 37 °C in 5% CO2 and passaged at 70–90% confluency with 0.05% TrypLE Express (all from ThermoFisher Scientific).
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bioRxiv - Genomics 2020Quote: ... Cell lysates dissolved in SDS load buffer were heated at 90°C for 3 min and separated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to a PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... These pieces were then washed in HBSS and dissociated in glia medium that consisted of 90% plating medium and 10% DNAse (Invitrogen). Both hemispheres of one brain were dissociated in 1 ml glia medium and plated in 10 ml of plating medium that was identical to rat primary culture plating medium ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... copper-catalyzed click reaction was performed by incubating the cells for 90 min at RT with freshly prepared labeling solution containing 10 µM Alexa Fluor 488 Azide (Invitrogen), 10 mM sodium ascorbate ...
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bioRxiv - Bioengineering 2021Quote: ... the medium was removed from the wells and replaced with 90 μL/well of Gibco CO2-independent medium (Life Technologies) + 10% FBS containing 1% v/v of cAMP GloSensor substrate stock solution (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... and 2mM PMSF) and heated to 98°C for 30 min and 90°C for 2h on a heat block (ThermoFisher). Samples were mixed well and centrifuged at 22,000 × g for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... mice were perfused 90 minutes after stimulation and brain sections were stained with rabbit anti-c-Fos antibody (1:500, Invitrogen). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI ...