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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... A pool of 4 siRNAs targeting mouse SNAP-47 and control Luciferase siRNA (Target Sequence: 5’-CGTACGCGGAATACTTCGA-3’) (Dharmacon, Thermofisher Scientific) were electroporated along with GFP into E15.5 cortical neurons (2 µg GFP + 50 pmol siRNAs) ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Immunology 2021Quote: ... The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyl phosphate p-toludine salt (NBT/BCIP chromagen) substrate solution (Thermo Scientific) for 7 min ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... maintained at 37° C and 5% CO2 and passaged every 3-4 days using trypsin-EDTA 0.05% (Life Technologies, Paisley, UK) to detach the cells ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... 3 hours later cultures were washed and fixed with 4% paraformaldehyde (Electron Microscopy Solutions) in phosphate-buffered saline (PBS; Thermo Fisher) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated glycoproteins were enriched by incubation for 3 hrs at 4°C on a rotating wheel with high affinity streptavidin agarose beads (ThermoFisher Scientific). Extensive washing was performed on a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: HSC cells atop ECM hydrogels subjected to the different treatments for 3 d were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 3 days (room temperature during the day and 4 °C at night) in PAT-NGS containing rabbit anti-GFP (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Eight days after subcutaneous immunisation of C57BL/6 mice with 50 μg of 4-Hydroxy-3-nitrophenyl acetyl hapten conjugated chicken gamma globulin (NP-CGG) in Imject Alum Adjuvant (ThermoFisher Scientific), 4.3 µg MAR hypoxia detecting probe (Goryo Chemical ...
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bioRxiv - Physiology 2024Quote: ... plus 1% penicillin/streptomycin in a humidified incubator at 37 °C and 5% CO2 and passaged every 3-4 days using 0.05% of Trypsin-EDTA (Thermo Fisher Scientific) to lift the cells from the culture dish ...
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bioRxiv - Molecular Biology 2023Quote: ... and blotted on two sides for 3 sec at 4 °C and 100% humidity before vitrified in liquid ethane using a Vitrobot Mark IV (Thermo Fisher). The grids were subsequently transferred to a Titan Krios (G3i ...
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bioRxiv - Cell Biology 2022Quote: ... 3.5 - 4 ml aliquots of the resulting 10,000xg supernatants were added up with 3-2.5 ml 1X PBS (Gibco, Carlsbad, CA, USA) to reach a final volume of 6.5 ml and were transferred on top of a 60% - 10% iodixanol gradient (Progen Biotechnik GmbH ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Microbiology 2022Quote: ... After 3 hours a fraction of the bacteria was fixed by the addition of 4% methanol-free paraformaldehyde (PFA, Thermo Scientific) for 10 min at room temperature and the reaction was quenched by the addition of 0.3 M glycine ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant bacmids were transfected into Sf9 cells to generate recombinant virus and amplified for large scale protein purification by growth to 3-4×106 cells/mL in Sf-900 II SFM media (ThermoFisher #10902088) in a humidified 28°C incubator shaking at 125 rpm ...
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bioRxiv - Microbiology 2024Quote: ... Samples were incubated at 95°C for 3 min and 10 µL were separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) in 1x MOPS buffer (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... the reaction mixture was dialyzed against deionized water at 4 °C for 2 days using Slide-A-LyzerTM Dialysis Cassette (10K MWCO, 3 mL, Thermo Scientific) and stored at −70 °C.
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bioRxiv - Immunology 2024Quote: ... 1 x 109 SupT1 CD4+ T cells were infected with 4 055 ng of VSV-G-HIVNL4-3 XCS HIV-Gagp24 for 3h in IMDM plus 10mM HEPES (Gibco/ThermoFisher Scientific) supplemented with 2 µg/ml of DEAE-dextran (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: The sample was blotted for 3 s with blot force 20 at 100% relative humidity and 4 °C using a Vitrobot Mark IV (Thermo Scientific). Grids were plunge frozen in liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Protein– antibody complexes were visualized using the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium for color development (Life Technologies).
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bioRxiv - Systems Biology 2024Quote: ... and incubated in total of 150 µL SH2 binding buffer containing 20 µM biotinylated SH2 domain on a rotator and 4 °C for 2-3 hours in low protein binding microcentrifuge tubes (1.5 mL, Thermo Scientific™). The functionalized magnetic beads are then washed twice in 1 mL of SH2 binding buffer ...
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bioRxiv - Bioengineering 2024Quote: Samples were washed 3 times with DPBS before being incubated with secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, 10125092) at 1:500 in the blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA was isolated from duplicate or triplicate samples and concentrations were measured using the NanoDrop One/One Microvolume UV-Vis Spectrophotometer (Thermofisher). CDNA was synthesized using the iScript cDNA synthesis kit (Biorad ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems, USA). Primers used in the study were designed using PrimerBlast tool of NCBI (Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... one-step quantitative real-time PCR was performed using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen) with primers specific for the TRS-L and TRS-B sites for the N gene as well as ACTB as an internal reference ...
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bioRxiv - Microbiology 2020Quote: ... One-step qRT-PCR was performed using the Invitrogen EXPRESS One-Step Superscript qRT-PCR kit (Thermo Fisher Cat #11781200) and the commercial Taqman probe ACE2 (Hs01085333_m1) ...
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bioRxiv - Systems Biology 2021Quote: ... The amount and purity of the RNA samples were quantified using a Nanodrop One/OneC Microvolume UV–VIS Spectrophotometer (ND-ONE-W) from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-PCR was performed using the Super-Script III One-Step RT-PCR System with Platinum Taq (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... one-step quantitative real-time PCR was performed using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen) with primers specific for the TRS-L and TRS-B sites for the N gene as well as ACTB as an internal reference ...
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bioRxiv - Cancer Biology 2022Quote: ... all exosome samples were diluted with one volume of Exosome Resuspension Buffer and one volume of 2X Denaturing Solution (both from Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed using One-Step Real-Time RT-PCR Master Mixes (Thermo Fisher Scientific, Waltham, USA) and the StepOnePlus Real Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). The one-step RT-PCR was performed using the forward primer Pol F1 5’-TACAGTGCAGGGGAAAGAATA-3’ (nt 4809-4829 ...
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bioRxiv - Microbiology 2023Quote: ... A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen). To amplify the pol region ...
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bioRxiv - Neuroscience 2023Quote: One million cortical cells and one million striatal cells were suspended in 6 mL D-minimum essential medium (DMEM, GIBCO) with 10% foetal bovine serum (DMEM+) ...
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bioRxiv - Immunology 2022Quote: LLC-MK2 cells were seeded in 2 × 96-well plates (one for inhibition and one for cytotoxicity) in complete media (DMEM from Gibco, Cat ...
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bioRxiv - Microbiology 2024Quote: ... and used to amplify a 204bp amplicon in a one-step RT-PCR reaction (SuperScript IV One-Step, Thermo Fisher) using custom primers (Table 1) ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...