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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were split 1:10 every 3 days by using trypsin (Gibco, 25200072).
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bioRxiv - Neuroscience 2022Quote: ... Cell nuclei were detected with TO-PRO-3 (1:400; Thermo Fisher Scientific). To analyze PNN structure and to detect synaptic proteins ...
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bioRxiv - Neuroscience 2021Quote: ... samples were then incubated with TO-PRO-3 (Thermo Fisher, T3605, 1:300), goat anti-rat Alexa Fluor 568 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... A final incubation of 3 min with DAPI (1:1000, Thermo Fisher Scientific) was done ...
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bioRxiv - Microbiology 2021Quote: ... followed by DNA staining with 1 µM TOPRO-3 iodide (642/661; Invitrogen) and staining of the fungal hyphae with 100 μg/ml Fluorescent Brightener 28 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were treated with DAPI (1:500, 3 min RT, ThermoFisher Scientific, D1306), washed briefly in PBS (2x) ...
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bioRxiv - Genetics 2021Quote: ... Nuclei were stained with TO-PRO-3 (Topro, Invitrogen, T3605; 1:3000 dilution).
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT with 3% BSA (Thermo Fisher) in tris-buffered saline with 0,1% Tween (TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in a 3:1 mixture of IMDM (ThermoFisher Scientific Cat. 12440053) and Ham’s F12 (ThermoFisher Scientific Cat ...
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bioRxiv - Microbiology 2021Quote: ... and primer sequences (Table 1) on a QuantStudio 3 platform (Applied Biosystems, USA). The relative expression levels of target genes were plotted as fold change compared with untreated or negative controls ...
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bioRxiv - Immunology 2020Quote: ... 100 μl) mixed with Alum (1:3 v/v) (ThermoFisher, Waltham, MA, USA). HBc-S was used as a control ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 mM 3’-d-GTP [>99% purity for UTP and CTP (ThermoFisher); ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase,” and thereby to stabilize ...
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bioRxiv - Biophysics 2022Quote: ... Cells were split 1:10 every 3 days by using trypsin (Gibco, 25200072).
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bioRxiv - Cancer Biology 2022Quote: ... incubated for 3 minutes in Hoechst nuclear stain (1:1000 in H2O, Thermofisher), and mounted with Vectashield (VectorLaboratories ...
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bioRxiv - Synthetic Biology 2022Quote: ... for assembly into levels 1 and 3 or 10 U BpiI (Thermo Scientific) for assembly into levels 2 in 20 μl 1x T4 ligase buffer (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... or streptavidin-HRP (1:1000 dilution, Fisher Scientific, Novex Cat# 43-423-3) to ensure that biotinylated and bait proteins were present ...
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bioRxiv - Cell Biology 2023Quote: ... INS-1 832/3 cells were transfected with 40 nM of Control (Ambion Silencer Select Negative Control #1 siRNA ...
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bioRxiv - Immunology 2023Quote: ... for 3 h and goat anti-rat AF 633 (1:200, Life technologies) for 1 h diluted in PBS containing 4 % BSA and 1 % goat serum at RT in a humidified dark chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... and primary antibodies for 3 days: chicken anti-GFP (1:750, Thermo Fisher Scientific Cat# A10262 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05% Tween-20 in PBS along with 1:1000 To-Pro-3 (Invitrogen) for 2h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... species-specific secondary antibodies (Life Technologies; 1:300 dilution in 3% BSA/PBS). Images were collected on a Zeiss Axioimager.D2 upright microscope using a 63x/Plan-neofluar objective and captured using a Coolsnap HQ2 camera (Photometrics ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 U/ml EPO (Zyrop 4000 IU injection) and 1× Pen-Strep (Gibco) as per established protocols ...
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bioRxiv - Biophysics 2023Quote: ... NucGreen™ Dead 488 (Invitrogen™, 1 drop per 3 ml in PBS); Alexa 647 Phalloidin (Invitrogen™ at 1/500) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA and lipofectamine (1:3 w/v) were diluted in Opti-MEM (Gibco) and incubated at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
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The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... The embryos were immunostained with TO-PRO-3 (Thermo Fisher; T3605; 1:1000) to identify the nuclei and images were captured using an A1 confocal microscope (Nikon) ...
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bioRxiv - Cancer Biology 2024Quote: ... and sgRNAs at a 1:3 molar ratio in Neon buffer R (Invitrogen) and incubating at room temperature for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... were performed with the Express one step RT-qPCR Universal kit (EXPRESS One-Step Superscript™ qRT-PCR Kit, universal Invitrogen) using 3.5µL of RNA and 6.5µL of RT qPCR mix and standard fast cycling parameters ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We did One-step real-time RT-PCR (Step-One Plus real-time PCR thermocycler, Applied Biosystems, Foster City, CA, USA), using total nucleic acids extracted by Trizol® (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... and the concentration was measured using a NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific ND-ONE-W). Amplification of 300 ng of DNA was performed using KOD Hot Start Master Mix (Sigma-Aldrich 71842) ...
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bioRxiv - Pathology 2024Quote: ... After one last freeze-thaw cycle the total protein content was measured on the NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Pathology 2024Quote: ... Kidneys were divided longitudinally with one half stored in formalin for histological evaluation and one half placed in RNALater (Thermofisher Scientific). Sham animals underwent a midline laparotomy alone with no renal pedicle clamping ...
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bioRxiv - Genetics 2023Quote: ... according to the manufacturer’s protocol and normalized to the correct concentration using the NanoDrop One Spectrophotometer (Thermo Scientific, ND-ONE-W). Samples were then submitted to the Center for Genome Sciences and Systems Biology (Washington University in St ...
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bioRxiv - Microbiology 2024Quote: ... extracted RNA was used as a template in a cycle-limited one-step RT-PCR reaction (SuperScript IV One-Step, Thermo Fisher) using custom primers (Table 2 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were cultured for 4 days with 1 µM 4-OHT in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with GlutaMax (Thermo Fisher Scientific), non-essential amino acids (NEAA ...
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bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... the mix was cooled down to 4°C and supplemented with 1 μl of a solution containing 4 U Terminal Transferase (Thermo Scientific), 0.1 μl 10X NEB 4 buffer (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug dilution series (1:3) were prepared in PDAC organoid culture medium containing 1 μg/ml Hoechst (Invitrogen) and 1 μg/ml PI (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Genetics 2022Quote: Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the tumoroids in a 1:3 and 1:10 effector:target (E:T) ratio in RPMI 1640 (Gibco) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... stained with TO-PRO-3 diluted 1:1000 in DPBS and 1 µM Hoechst 33342 (Thermo Fisher 62249) nuclear counterstain for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 3 μg mRNA at a mass ratio of 1:1 using Lipofectamine MessengerMAX (Invitrogen) according to the manufacturer’s guide ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... cells were stained by BODIPY™ FL C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific #D3922) to determine the amount of lipid droplet in regular conditions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... 1.5×107 cells were incubated for 4 hours with 3 μL of AHA diluted in DMSO (Acros Organics via ThermoFisher Scientific, Germany) to final concentrations of 0 ...
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bioRxiv - Microbiology 2020Quote: ... 1.5×107 cells were incubated for 4 hours with 3 μL of AHA diluted in DMSO (Acros Organics via ThermoFisher Scientific, Germany) to final concentrations of 0 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then placed again into the Cytofunnel and spun at 800 RPM for 3 min using a Cytospin™ 4 Cytocentrifuge (Thermo Fisher). For intracellular visualization of IgM ...