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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Insulin Receptor β CT-3 mouse monoclonal antibody (1:1000, AHR0271, ThermoFisher), Perilipin-1/PLIN1 D1D8 rabbit monoclonal antibody (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... cells were passaged 1:15 every 3 days using trypsin (Gibco, 25300054) and centrifugations at 200 xg ...
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bioRxiv - Cell Biology 2024Quote: ... and replaced with M2 containing 1 μM TO-PRO-3 iodide (ThermoFisher). A SpectraMax i3X multimode detection platform equipped with a MiniMax cytometer (Molecular Devices ...
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bioRxiv - Plant Biology 2023Quote: ... and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen), 2 μL GlycoBlue (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... transfection reagent at a ratio of 1:3 DNA:Fugene in optiMEM (Gibco). SARS-2 PV were harvested at 48h post transfection and supernatant filtered through a 0.45 μm acetate cellulose filter (Starlab ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei were counterstained with TO-PRO™-3 Iodide (Invitrogen, 1:2000). For immunofluorescence detection of DEK or PCNA cells were fixed with 4% PFA/PBS (20 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and extracted total RNA using BCP (1-bromo-3-chloropropane; Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... mouse anti-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:50000, Thermo Fisher, AM4300), mouse anti-CASQ1 (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... following a 3-hours treatment with 100 ng ml−1 colcemid (GIBCO) cells were trypsinized and recovered in a falcon tube ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were incubated with TO-PRO-3 Iodide (1:1000, Life Technologies) in 1x PBS for 20 minutes and then mounted with VectaShield mounting media (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... Synthesized cDNA was diluted 1:3 and QS5 or QS6 (Life Technologies) systems were used for performing RT-qPCR analyses ...
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bioRxiv - Immunology 2024Quote: ... at a DNA mass ratio of 3:1 in Opti-MEM (ThermoFisher). After a 10 minute incubation ...
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bioRxiv - Neuroscience 2024Quote: ... TO-PRO-3 (1:3000; Thermo Fisher Scientific, cat. #T3605, Waltham, USA) or DAPI (1:2000 ...
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bioRxiv - Biophysics 2024Quote: ... were cleaned and silanized with 1% 3-aminopropyl-trimethoxysilane solution (Acros Organics) for 10 min and then treated with 0.5% glutaraldehyde (Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The mounting slide (3’’ x 1’’ x 1mm, Fisher Scientific, Pittsburgh, PA) was placed on top of the cover slide to finish preparation ...
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bioRxiv - Biophysics 2024Quote: ... Concentration of RyR1 in the combined fractions was measured with a Nanodrop One spectrophotometer (Thermo Scientific; 1 abs @ 280 nm = 1 mg/mL). After incubation ...
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bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated for one hour with AlexaFluor®-conjugated donkey secondary antibodies (1:500, Thermo Fisher Scientific) and finally counterstained with Hoechst 33342 (20 μm ...
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bioRxiv - Microbiology 2020Quote: SAV3 RNA from heart tissue was quantified with a modified one-step nsP-1 assay (Ag-Path, Ambion) (Jiraporn Jarungsriapisit et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... one volume of chromatin was mixed with 1/10 volume of Dynabeads M-270 Epoxy (ThermoFisher, LOT 01063817) coupled to immunoglobulin G (IgG ...
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bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was measured spectrophotometrically (ε280 21430 M-1cm-1, MW 24.79 kDa) using NanoDrop One (Thermo Scientific). Dialysed MSP1D1 was concentrated to 5 mg/ml using a 10 kDa MWCO Amicon Ultra centrifugal concentrator (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... and 10 ml of 1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Life Technologies). Sf9 insect cells (ATCC CRL-1711 ...
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bioRxiv - Neuroscience 2022Quote: ... probenecid (1:250) and PowerLoad concentrate (1:100) (Fluo-4 Calcium Imaging Kit, Molecular Probes) in BrainPhys basal media for 30 min (37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was visualized using 1 µg ml-1 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen). Acrosomes were visualized using 0.5 µg ml-1 lectin peanut agglutinin (PNA ...
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bioRxiv - Bioengineering 2022Quote: ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 1 M was purchased from Gibco (Waltham, MA), sodium chloride 5 M was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2024Quote: ... Serum samples were assayed at 3-fold dilutions starting at a 1:3 dilution in Blocker Casein in PBS (Thermo Fisher Scientific) diluent ...
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bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by transfer to a positively charged nylon membrane and then crosslinked with EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) at 60°C for 1–2 hours and prehybridized with ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen, cat # AM8670). The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen ...
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bioRxiv - Immunology 2022Quote: ... The 40 mg/ml 4-HT stock solution was diluted 1:1 in Kolliphor EL (MilliporeSigma) 59.Before injection the solution was further diluted 1:3 in PBS (Gibco) and warmed to 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Claudin-4 (1:200, Thermo Fisher Scientific, 36-4800), rabbit anti-Hopx (1:100 ...
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bioRxiv - Bioengineering 2021Quote: ... and ethidium homodimer-1 (4 µM, stains dead cell nuclei, Invitrogen) for later timepoints.
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 1 μM Fluo-4-AM (Thermo Fisher) in culture medium and incubated at 37 °C and 5% CO2 for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... were used at 4°C overnight: anti-GFP (1:250, Invitrogen), anti-DsRed(RFP ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal anti-Claudin-4 (1:100, Novex, Life Technologies, USA) and rabbit polyclonal anti-claudin-4 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:4 dilution of trypsin was used (Gibco, Cat# 15400054) to split the cells into thirds.
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bioRxiv - Bioengineering 2022Quote: ... eBCs were stained with polyclonal Synaptotagmin-4 antibody (1:50; Invitrogen) and incubated at 4 °C overnight ...