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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (NBD-PE) was purchased from ThermoFisher (Waltham, MA). Cholesterol Oxidase from Streptomyces was purchased from MP Biomedicals (Santa Ana ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The cells were passaged every 3-4 days after they achieved confluency of 80-90% using Trypsin 0.25% EDTA (Life Technologies).
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Neuroscience 2022Quote: ... the cell cultures were washed 3 times with 37 °C DPBS+ and fixed using 4% w/v paraformaldehyde (PFA; Affymetrix) in PBS for 2.5 h ...
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bioRxiv - Genetics 2022Quote: ... LC-MS/MS based proteomics datasets (4 groups × 3 replicates) were then generated in a lab (code: NVG) using Q Exactive HF-X mass spectrometer (ThermoFisher). To eliminate confounding factors such as experimental sample-processing order with sample group ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... islets from 3-4-week-old control and β-LonP1KO mice were handpicked and then dispersed with trypsin (Thermo Scientific). Islet cells were counted and incubated with adenovirus expressing an empty vector control (Ad-RIP2 EV ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Pathology 2024Quote: ... retinas were post-fixed in 4% PFA for 3 minutes at room temperature before mounting in Prolong Gold antifade medium (Invitrogen). Images were acquired using a Zeiss LSM710 and Zeiss LSM880 confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... 30 to 50 μg of protein extracts were resolved by SDS-polyacrylamide gel electrophoresis using 4–12% NuPAGE Bis-Tris gradient gel or 3–8% Tris-acetate gel and run (Invitrogen) and MOPS or Tris-acetate running buffer (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: ... red blood cells were removed by adding 1 ml ACK lysis buffer (150 mM NH4Cl and 10 mM KHCO3) and incubated on ice for 3 minutes before washing with HBSS containing 4% fetal bovine serum (HI FBS, Gibco). During the optimization of the protocol outlined above ...
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bioRxiv - Genetics 2024Quote: ... Proteins were loaded and run on a 4-12% Bis-Tris pre-cast gel or a 3-8% Tris-Acetate pre-cast gel (Invitrogen) and were electrophoretically transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and snap-frozen in chilled 2-methyl butane (Acros Organics, Fisher Scientific, Pittsburgh, PA). Tissue blocks were sectioned (20 μm ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and snap-frozen in chilled 2-methyl butane (Acros Organics, Fisher Scientific, Pittsburgh, PA). Tissue blocks were sectioned (20 μm ...
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bioRxiv - Cell Biology 2021Quote: Mitochondrial membrane potential was tested using the fluorescent potentiometric compound tetramethylrhodamine methyl ester (TMRM, 20 nM; Invitrogen). Cells were incubated in DMEM without phenol red with 0.1% FBS and CsH (1 µM ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondria labeling was achieved by adding 2 µL of a tetramethyl rhodamine methyl ester (TMRM, ThermoFisher, T668) directly to the medium to achieve a final concentration of TMRM of 100 nM ...
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bioRxiv - Neuroscience 2022Quote: ... the brains were rapidly frozen in dry ice-cooled 2-methyl butane (Fisher Scientific, Hampton, NH, USA). Coronal sections of the VTA at 10⍰μm were obtained using a cryostat and mounted onto superfrost slides (Fisher Scientific Ltd ...
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bioRxiv - Microbiology 2024Quote: ... then PEGylated in situ using the methyl-PEG-NHS-ester reagent MS(PEG)12 (#22685, ThermoFisher Scientific). PEGylation was performed to reduce preferred orientation ...
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bioRxiv - Microbiology 2024Quote: ... The extracted fatty acid methyl esters were analysed using gas chromatography (Trace GC Ultra, Thermo Scientific, Germany) coupled to a mass spectrometer (ISQ ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected and 48 later incubated for 40min with 100nM tetramethylrhodamine methyl ester (TMRM) (#T668, Thermofisher), a fluorescent lipophilic cation ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.4) for 10 min and later incubated with 4 uM fluo-4/AM (1 mM, Molecular probes, #F14201) and 0.02% pluronic F-127 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
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bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
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bioRxiv - Cell Biology 2022Quote: ... with a one-step RT-PCR kit using Taqman® technology (AgPath-IDTM One-Step RT-PCR, Life Technologies) and using the Applied Biosystems ViiA 7 Real-Time PCR System and the appropriate primers for Taqman assays (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... To determine RNA purity and concentration we used the NanoDrop One/OneC spectrophotometer (ThermoFisher, Cat. No. ND-ONE-W). Then ...
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bioRxiv - Biophysics 2021Quote: ... at a ratio of 1:1.5:1.75:2 (FAM155A-3×FLAG:NALCN-1077-3×HA-GFP:UNC79-3×FLAG:UNC80-3×FLAG) using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 40-48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-centrin-3 (1:1000; PA5-35865; Thermo Scientific, Rockford, USA), rabbit anti-acetylated α-tubulin (1:800 ...
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bioRxiv - Cell Biology 2020Quote: ... 1% [w/w] P/S [Gibco] and 1 % [w/w] Glutamax [Gibco]) supplemented with 1 μM TO-PRO®-3 (Thermo Fisher Scientific). After 16 h ...
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bioRxiv - Immunology 2021Quote: ... 3 μl of a 1:400,000 dilution of ERCC synthetic mRNAs (ThermoFisher) were added and total RNA was isolated using a Quick-RNA MicroPrep Kit (Zymo Research ...
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bioRxiv - Neuroscience 2022Quote: ... and nuclear dye (TO-PRO-3 Iodide, 1:400, Thermo Fisher T3605) were added to the blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... and a 1:400 dilution of TO-PRO-3 (Thermo Fisher T3605) for 24 hr at 4°C in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... and rabbit anti-activated Caspase-3 (Fisher Scientific/BD, BDB559565, 1:500). Antibody used for fluorescent in situ hybridization was mouse anti-Dig (Jackson ImmunoResearch 200-002-156 ...
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bioRxiv - Microbiology 2021Quote: ... to a DNA:lipofectamine ratio of 1:3 and 100μl Opti-MEM (Gibco). Cells were collected 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-phospho-Pak1-2-3 (pSer141) (44-940G, 1:2000) from Invitrogen/Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Cell Biology 2021Quote: ... phospho-PAK1/2/3 (Thr402) (ThermoFisher PA1-4636, 1:1000 for WB), phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560 ...
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bioRxiv - Microbiology 2022Quote: Calu-3 cells were maintained in Opti-MEM I (1) + GlutaMAX (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: Calu-3 cells were cultured in Opti-MEM I (1) + GlutaMAX (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells were cultured in Opti-MEM I (1) + GlutaMAX (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 cells were maintained in Opti-MEM I (1) + GlutaMAX (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... in Retinal Differentiation Media (DMEM/F12 nutrient mix (3:1 ratio, Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... After 1–3 h incubation with Alexa Fluor-conjugated secondary antibodies (Invitrogen) followed by DAPI staining (0.1 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... TO-PRO™-3 iodide (Thermo Fisher; #T3605; 1:1,000 in PTwH.) As secondary antibodies donkey anti-rabbit Alexa Fluor® 568 (Thermo Fisher ...
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bioRxiv - Paleontology 2021Quote: ... and finally stained with 1 μM To-Pro-3(Invitrogen, CA, USA) for 30min ...
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bioRxiv - Microbiology 2023Quote: ... transfection reagent at a ratio of 1:3 DNA:Fugene in optiMEM (Gibco). SARS-2 PV were harvested at 48h post transfection and supernatant filtered through a 0.45 μm acetate cellulose filter (Starlab ...