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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the catalysis of γ-glutamyl-3-carboxy-4-nitroanilide was measured at light absorbance at 410 nm using spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 1x TBS-T with 3% BSA (Fisher Scientific, BP1600): α-CD11b (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed 3 times in warm PBS and fixed for 45 min at room temperature in 4% (w/v) formaldehyde (Thermo Scientific; 28908) in PBS containing 0.1% Triton-X100 ...
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bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged as aggregates every 3-4 days until they reach 60-70% confluency with 0.5mM EDTA diluted in PBS (Thermofisher, Canada. Catalog#15575020).
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was replaced with 100 µL full melanoma medium with 4 µM Caspase-3/7 activity dye (CellEvent, Thermo Fisher Scientific). Plates were then imaged on a Celigo Imaging Cytometer (Nexcelom ...
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bioRxiv - Microbiology 2020Quote: ... The grids were blotted for 3-4 s and plunged into an ethane/propane mixture using a vitrification apparatus (Vitrobot, Thermo Fisher Scientific). For Fab 4G2-treated VLP EM sample preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were immunized by injection of OVA coupled to the hapten 4-hydroxy-3-nitrophenylacetyl (NP-OVA) precipitated in alum (Imject® Alum, Thermo Scientific) into the hind footpads (25ul) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
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bioRxiv - Genetics 2020Quote: ... 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene 3-dodecanoic acid (BODIPY™ FL C12; Thermo Fisher Scientific, USA) (4 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: N2A cells were transfected as described above with BicD2-KIF tail fusions and MBNL-GFP proteins at a ratio of 2:3 in 4-well chamber slides (Thermo Fisher, 154526). 24 hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were passaged every 3-4 days once reaching 70-80% confluency using pre-warmed Versene (Fisher Scientific #15-040-066).
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... (3) 65%-95% for 5 min and (4) washout for 15 min) using the EASY-nano LC 1200 system (Thermo Fisher Scientific) with a flow rate of 300 nL/min ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: HTM cells cultured atop ECM hydrogels in presence of the different treatments for 3 days were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... We used siDESIGN Center (Horizon Discovery) to design siRNA (siUNG#4) targeting the 3′UTR region of UNG mRNA (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then centrifuged at 1000rpm for 3 minutes at 4°C before being suspended in 100μl HBSS (ThermoFisher, Cat No. 14025) + 1% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... followed by mechanical blotting for 3-4 s and rapid vitrification in liquid ethane with a Vitrobot Mark IV plunge-freezing device (Thermo Fisher Scientific) operated at 4 °C and 100 % relative humidity.
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bioRxiv - Molecular Biology 2023Quote: ... 7H11 plates contained 50 μg/mL hygromycin and 50 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) (Thermo Scientific). Unless specified ...
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bioRxiv - Molecular Biology 2023Quote: ... P8 pups were fed 1 μl 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid BODIPY™ FL C16 (BODIPY FL C16, ThermoFisher Scientific, D3821) diluted in olive oil at a concentration of 10 μg/μL ...
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bioRxiv - Neuroscience 2023Quote: ... before being spun (5000rpm 4⁰C for 3 minutes) and homogenised (70 seconds with a pestle) in 300 ul trizol (Invitrogen 15596-026). RNA was extracted using the Zymo Direct-zol RNA Microprep Kit (Zymo Research ...
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bioRxiv - Pathology 2023Quote: Dried peptide samples were dissolved in 4.8 μL of 0.25% formic acid with 3% (vol/vol) acetonitrile and 4 μL of each sample was injected into an EasynLC 1000 (Thermo Fisher Scientific). Peptides were separated on a 45 cm in-house packed column (360 μm OD×75 μm ID ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from n = 3–4 biological replicates per sample using the PureLink RNA Mini Kit (Invitrogen, Waltham, MA, USA), and sample quality was evaluated using a TapeStation ...
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bioRxiv - Cell Biology 2023Quote: ... Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific), then transferred to 0.45 µm nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... cells were washed in Ca2+-free PBS and incubated for 20 min at room temperature with 3 μM Fluo-4-AM (F14201, Thermo Fisher Scientific) in PBS while being protected from ambient light ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts started to migrate out of the skin biopsies after 7-10 days and were transferred to two T75 flasks after 3-4 weeks using the standard splitting protocol with TrypLE™ Express enzyme (Thermo Fisher). Fibroblasts were then cultured in IMDM (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Uterine tissues were cut into 3- to 4-mm lengths with sterile scissors and incubated in phenol red-free HBSS(−) containing dispase (#17105-041, Thermo Fisher Scientific) and pancreatin (#P3292 ...
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bioRxiv - Neuroscience 2023Quote: ... for a 35 mm dish 4 μl of Lipofectamine 2000 and selected plasmids (3 μg) were diluted in Opti-MEM medium (Thermo Fisher Scientific) to a final volume of 250 μl and incubated for 20 min at RT ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Neuroscience 2024Quote: ... Adult brains were post-fixed for up to 24 hours with 4% paraformaldehyde and cryosectioned after 2-3 days of incubation in 30% sucrose (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples were resolved on NuPAGE 4–12% Bis-Tris gels with MOPS buffer or 3–8% Tris-acetate gels with Tris-acetate buffer (Life Technologies, #LA0041) and then transferred to 0.45 μm nitrocellulose membrane (Amersham) ...
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bioRxiv - Immunology 2024Quote: ... Grids were either blotted for 3 or 4 s before being plunged into liquid nitrogen-cooled liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific).
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bioRxiv - Synthetic Biology 2024Quote: ... of 3-4 months based on previous established methods188 and maintained at 37°C and 5% CO2 in DMEM (ThermoFisher, MA, USA), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... The lysates were incubated for 3 h at 4°C under gentle rotation with Pierce Protein G Plus Agarose (Thermo Fisher Scientific) conjugated to antibodies specific for FUS (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2024Quote: ... 17,22,43,44 Early passages (P2-4) of cells from 3 rats were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Thermo Scientific ...
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bioRxiv - Biophysics 2024Quote: ... were then blotted for 3 to 6 s at 100% humidity (4 °C) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected in counted superresolution bin 2 mode on a Titan Krios G3 (FEI ...
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bioRxiv - Biochemistry 2024Quote: ... it was centrifuged 3-5x at 1,300 x g (5 min, 4 °C, Heraeus multifuge X3R, Thermo Scientific, rotor F15-8×50cy) until no pellet was visible ...
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bioRxiv - Immunology 2024Quote: ... 1 x 109 SupT1 CD4+ T cells were infected with 4 055 ng of VSV-G-HIVNL4-3 XCS HIV-Gagp24 for 3h in IMDM plus 10mM HEPES (Gibco/ThermoFisher Scientific) supplemented with 2 µg/ml of DEAE-dextran (Sigma) ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was then probed with specific primary antibodies diluted 1:1000 in antibody dilution buffer (3% BSA in TBS-T) and incubated overnight at 4°C - either anti-TNFR1 rabbit monoclonal antibody (Invitrogen, #MA5-14899) or anti-β-Actin mouse monoclonal antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... proteolytic peptides (500 ng for Experiment 3 and 400 ng for Experiment 4) were loaded on Acclaim PepMap 100 C18 trap column (75 µm x 20 mm, 3 µm particle size; Thermo Fisher Scientific) for 10 min at 2 µL/min with 100% solvent A ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) at 37 °C for 5 min in PBTX solution ...
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with Fluo-4 AM (1 mM; Molecular Probes, Invitrogen) in incubation buffer for 30 min at 37°C and washed (20 min) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with Fluo-4 AM (1 mM; Molecular Probes, Invitrogen) in incubation buffer for 30 min at 37°C and washed (20 min) ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Elavl3/4 (Same as HuC/D, 1:500, Invitrogen Molecular Probes, A21271), Activated Caspase-3 (1:200 ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Elavl3/4 (Same as HuC/D, 1:500, Invitrogen Molecular Probes, A21271), Activated Caspase-3 (1:200 ...
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bioRxiv - Bioengineering 2020Quote: ... and a slide with 4 um fluorescent beads (FocalCheck #1, Fisher Scientific), respectively.