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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... filtered through a 70 µm nylon colander (Falcon) with 3-4 ml of Ca2+- and Mg2+-free HBSS medium containing 10 % fetal bovine serum (FBS, Gibco), 1 % MEM-vit (Gibco ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... Samples were boiled at 95ᵒC for 3 minutes to denature proteins and separated on a 4-12% NuPage Bis-Tris gel (Invitrogen, NP0322BOX). Proteins were transferred using an iBlot Gel Transfer device (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
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bioRxiv - Molecular Biology 2021Quote: ... COCS were incubated for 3 days at 4°C with secondary antibodies Alexa594-conjugated goat anti-rabbit IgG (Life Technologies) or Alexa647-conjugated goat anti-rat IgG (Life Technologies ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Genomics 2020Quote: ... Cell lysates dissolved in SDS load buffer were heated at 90°C for 3 min and separated on a Novex WedgeWell 4-12% Tris-Glycine Gel (Invitrogen). Proteins were transferred to a PVDF Transfer Membrane (Thermo Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were then centrifuged at 300 g for 3 min and fixed with 4 mM 3,3’– dithiobis(sulfosuccinimidyl propionate) (DTSSP, Life Technologies) in PBS at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resolved on NuPage 3-8% w/v Tris-Acetate or 4-12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: Group 3 MB cells (3×105) were seeded overnight and subsequently serum-starved for 4 hours prior to transient transfection using Lipofectamine 2000 (Invitrogen) for 6 hours in serum-free media with miR-212-3p mimic (25 nM and 50 nM ...
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bioRxiv - Cell Biology 2021Quote: Extracts were analyzed by SDS–PAGE using a 4-12% Bis–Tris or 3-8% Tris-Acetate NuPAGE gels (Invitrogen) and transferred onto PVDF membranes (Millipore) ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM MEK inhibitor PD0325901 (Wako, Tokyo, Japan), 3 μM GSK3 inhibitor CHIR99021 (Wako, Tokyo, Japan) and Penicillin-Streptomycin (Invitrogen). Blastocyst outgrowths were disaggregated and passaged onto the new wells plated with feeder cells in the same medium condition ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were submitted to an SDS-PAGE protein separation in 4-12 % or 3-8 % (depending on the molecular weight of the protein of interest) NuPAGE bis-tris gels (Invitrogen) and transferred to a nitrocellulose membrane (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... DTT concentration in the buffer was reduced by dialysis against TEDG buffer with DTT 0.1 mM at 4 ºC overnight using a 3 KDa pore size dialysis cassette (ThermoFisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... reinhardtii cells were grown at room temperature for 3-4 days in the light in 20 mL of TAP media (ThermoFisher). The algae were collected by centrifugation at 10,000 x g for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins were briefly subjected to SDS-PAGE (3 min at 200 V, 4– 12% Bis-Tris gel, Life Technologies) and stained with InstantBlue Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 1.3163 g/cm3) and ultracentrifuged for 3 hours at 180,000 × g (average) at 4°C (TH-641 rotor, Thermo Fisher, thinwall polypropylene tube with 13.2 ml capacity ...
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bioRxiv - Cell Biology 2021Quote: ... and equal concentrations of each sample were separated on a 3-8% Tris-Acetate or 4-12% Bis-Tris gel (Invitrogen) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with RNAScope H2O2 for 4 min at RT and subsequently washed 2 x 3 min with UltraPure Distilled Water (Invitrogen) at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Biophysics 2022Quote: ... were washed three times in sterile PBS and opsonized overnight at 4°C in 3 mg/mL mouse IgG (Invitrogen). To remove excess antibody ...
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bioRxiv - Microbiology 2020Quote: ... or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16, Thermo Fisher Scientific) in a modified SC medium ...
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bioRxiv - Plant Biology 2021Quote: ... TRV:NRC2/3/4 plasmid (Wu et al., 2016; Wu et al., 2017) by Phusion High-Fidelity DNA Polymerase (Thermo Fisher) using primers listed in SI Appendix ...
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bioRxiv - Physiology 2022Quote: ... then perfused the lungs with 3 mL of ice-cold DPBS containing Ca2+ and Mg2+ followed by 5 mL of 4% methanol-free formaldehyde (ThermoFisher) in DPBS containing Ca2+ and Mg2+ ...
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bioRxiv - Cell Biology 2020Quote: Eluted proteins were briefly subjected to SDS-PAGE (3 minutes at 200 V, 4-12% Bis-Tris gel, Life Technologies), and stained with InstantBlueTM Coomassie protein stain before being washed with ddH2O overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were rinsed thrice in PBS and individually incubated with MCDB131 growth medium supplemented with 4 µM Fluo 3-AM (Invitrogen) for 25 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... and 143B cybrid cells were passaged every 3-4 days at 80% confluence and trypsinised using 0.25% trypsin-EDTA (Gibco #25200056). Similarly ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated in 4-12% Nu-Page Bis-Tris (FANCA analysis) or NuPAGE 3-8% Tris-Acetate (FANCD2) polyacrylamide gels (Invitrogen) and transferred to nitrocellulose membranes (Amersham Biotech ...
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bioRxiv - Immunology 2021Quote: ... A force of -4 was used to blot excess liquid for 3 s using a Vitrobot Mark IV (Thermo Fisher) followed by plunge-freezing with liquid ethane ...
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bioRxiv - Immunology 2020Quote: ... The excess liquid was blotted for 3 seconds with a force of −4 using a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: ... were crossed for 2-3 days and then transferred to fly food made from 0.6 g of Carolina Formula 4-24 (Fisher Scientific) and 2 mL of ddH2O ...
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bioRxiv - Microbiology 2020Quote: ... the same amounts of SOSIP trimer and SOSIP-I53-50NP were loaded on a 4-12% Bis-Tris NuPAGE gel or 3-12% Bis-Tris NuPAGE gel (both from Invitrogen), respectively.
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bioRxiv - Microbiology 2023Quote: ... was used for induction of gene expression and X-gal (X-Gal 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside; Thermofisher) TSA plates were used for bacterial assessment ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... For differential glucose concentration treatments between day 2 and 3 and between day 3 and 4, N2B27 was made using DMEM/F12 without glucose (L0091-500, Biowest) and Neurobasal without glucose (A2477501, ThermoFisher). For 1X and 3X glucose concentrations ...
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bioRxiv - Immunology 2023Quote: Following euthanasia, adult zebrafish heads (15 wpf, N=3) were fixed in a solution of 4% methanol-free formaldehyde (Thermofisher) in 60 mM HEPES buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell bodies were removed by centrifugation (1,150 g, 3 min, 4°C; Sorvall Legend XTR, Thermo Fisher Scientific). A sucrose cushion (10 ML of 25% sucrose in HMS ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% CO2 in a humidified incubator and were passaged every 3-4 days using 0.05% Trypsin-EDTA (ThermoFisher Scientific, 25300). Stable U2OS-derived cell lines ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Neuroscience 2022Quote: ... slices for optical recordings were incubated for 30 minutes in oxygenated Krebs solution added to a 3% Di-4-ANEPPS (Invitrogen) stock solution and mixed with an equal volume of fetal bovine serum (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... mRNA samples of 4 ng were subjected to DNaseI treatment and 3’ dephosphorylation using FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) and T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred to a 15 mL column containing 3 mL Ni-NTA Agarose affinity resin equilibrated with buffer A at 4 °C (Invitrogen). The column was washed using a gradient of Buffer A containing 20 mM ...
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bioRxiv - Genomics 2024Quote: ... and transfer plasmids were transfected at a mass ratio of 2:3:4 with Lipofectamine 3000 (Thermo Fisher Scientific L3000001) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Molecular Biology 2022Quote: ... The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells, 288 reactions in 3×96-well plates) following manufacture’s protocol (Invitrogen C862003). After transformation ...