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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... and a Step-One PCR instrument (Applied Biosystems) were utilized for the qRT-PCR reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... Step-one Real-time PCR system (Life Technologies) or Bio-Rad C1000 Thermal Cycler (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and one-part DMEM/F12 (Fisher Scientific; 11320033) supplemented with 5 μg/ml insulin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... coli One Shot BL21 Star (DE3) cells (Invitrogen). The unlabeled expression was carried out in the Terrific Broth medium (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Step-One Plus thermal cycler (Thermo fisher scientific), using Power Up SYBR green master mix (containing STBR green as reporter dye) ...
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bioRxiv - Microbiology 2024Quote: ... with the NanoDrop One spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... supplemented with one mM sodium pyruvate (Gibco 11360070), two mM GlutaMAX™ (Gibco 35050061) ...
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bioRxiv - Immunology 2024Quote: ... supplemented with one mM sodium pyruvate (Gibco 11360070), two mM GlutaMAX™ (Gibco 35050061) ...
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bioRxiv - Pathology 2024Quote: ... Conjugation efficiency was verified by NanoDrop One (ThermoFisher). All antibody concentrations were titrated.
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bioRxiv - Molecular Biology 2024Quote: ... One hundred µL of Alamar Blue® (Thermofisher) was added into each well ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA content was quantified using NanoDrop One (ThermoFisher), diluted with 25 mM NaPO4 (pH 6.5 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1x Culture One supplement (Thermofisher #A33202-01) along with the neurotrophic factors 20 ng/mL BDNF (Peprotech #450-02) ...
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bioRxiv - Genomics 2024Quote: ... one part of Medium 199 (22340-020, Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... with 10% fetal bovine serum (Gibco One Shot).
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bioRxiv - Cancer Biology 2024Quote: ... quantified by NanoDrop One C (Thermo Fisher Scientific) and reverse-transcribed to cDNA with Maxima First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was quantitated using Nanodrop one (Thermo Scientific) then converted to cDNA (RevertAid Reverse Transcriptase ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was quantitated using Nanodrop one (Thermo Scientific) and tapestation ...
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bioRxiv - Developmental Biology 2024Quote: ... using CellsDirect One-Step qRT–PCR Kits (ThermoFisher). Pre-amplification was performed in a Veriti Thermal Cycler (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were washed in 3 times in 3% PBST (10 min) and incubated in goat anti-chicken Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in blocking buffer at room temperature for 2hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16°C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3).
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was then visualised using 1 µg ml-1 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) or 4 µM TO-PRO-3 Iodide (TOPRO ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 1 μm FluoSpheres (1 to 800 dilution, 585/608 nm, ThermoFisher Scientific, F13083), were applied to the grid in the humidity chamber of the Leica EM GP plunger prior to the vitrification step ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were split 1:4-1:12 using Versene Solution (Thermo Fisher Scientiûc, Weltham, MA, USA) 2-3 times per week ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run using primer/probes at 1:20 dilution and Taqman FastVirus one-step reaction mix (Thermo Scientific), and amplified using an Applied Biosystems QS3 cycler ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed three times with PBS and incubated for one hour with secondary antibody (Alexa Fluor 594, chicken anti-mouse 1:1’000, Invitrogen) in PBS with 0.1 % Triton X-100 and 6 % FBS ...
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bioRxiv - Genomics 2021Quote: ... Samples were transferred to 1.5 mL safe-lock tubes containing one scoop of acid-washed glass beads (<106μM) and 1 mL TRIzol (Invitrogen) containing 20μg/mL GenElute-LPA (Sigma-Aldricht ...
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bioRxiv - Neuroscience 2022Quote: ... and one or more of the following antibodies: goat anti-rabbit IgG Alexa Fluor 568 (1:1000, #A11036, Invitrogen), goat anti-mouse IgG Alexa Fluor 647 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.1%TBS-Tween for one hour and incubated with anti-Biotin (Invitrogen, Cat # 31852, RRID: AB_228243, 1:1000) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies for one series included rabbit anti-beta Amyloid (1:300, Cat# 715800, Lot# SH257822; Invitrogen, Waltham, MA). Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500 ...
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bioRxiv - Immunology 2022Quote: ... After one washing step (1% BSA/PBS) cells were permeabilized in PermBuffer (saponin-based permeabilization and wash buffer; ThermoFisher) for 15min and subsequently stained with pAKT Ser473 (CellSignaling ...
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bioRxiv - Neuroscience 2020Quote: ... and AMG44 (final concentration: 5μM) treatment one-hour after plating in cell media (DMEM/F12 [Gibco, 10565018], 1% N2 [Gibco, 17502048] ...
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bioRxiv - Molecular Biology 2021Quote: Cells were lysed by adding one volume of TNES buffer (TNE + 1% SDS) supplemented with RNaseA (Invitrogen cat #12091021) at final concentration of 100 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 1:2000) and secondary antibody for one hour (Optogenetics experiment: Alexa Fluor 488 goat anti chicken, Invitrogen (cat# A11039), 1:400 ...
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bioRxiv - Physiology 2023Quote: ... and one of the following secondary antibodies: Donkey anti-rabbit Alexa Fluor 488 (Invitrogen; Catalog# A21206; 1:250 dilution), Donkey anti-goat Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for one hour at room temperature (1:1000, Alexa Fluor 488 Goat anti-Mouse IgG, Invitrogen A-11001), and similarly washed ...
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bioRxiv - Neuroscience 2023Quote: ... USA) and incubated for one hour at room temperature with Streptavidin Alexa Fluor™ 568 conjugate (1:500)(Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular DNA was extracted with one volume of phenol:chloroform:isoamyl alcohol (25:24:1, #15593031, ThermoFisher Scientific, Waltham, MA, USA), precipitated with one volume of isopropyl alcohol ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were incubated in one of the following primary antibodies overnight: rabbit anti-KCNQ2 (1:1000, ThermoFisher PA1-929), rabbit anti-KCNQ3 (1:400 ...
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bioRxiv - Cell Biology 2024Quote: ... secondary antibodies were added for one hour at room temperature for either Alexa-488 or Alexa-568 (1:1,000, Invitrogen). Cells were further washed 4 times with PBS for 5 minutes each before mounting using DAPI mounting medium (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... and CAF-1 p60 (5′-AAUCUUGCUCGUCAUACCA-3′) were transfected using RNAi MAX (Invitrogen).
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... and TO-PRO®-3 (1:3000, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: JEG-3 cells were infected with ZIKV for 1 h in DMEM (Gibco) with 2% FCS at an MOI of 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were detected with TO-PRO-3 (1:400; Thermo Fisher Scientific). The retinal and optic nerve slices were analyzed with a confocal laser-scanning microscope (LSM 510 META ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μl of sample on a 1 mm thick glass slide (Fisher Scientific) and an 18 x18 mm cover slip (VWR ...
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bioRxiv - Molecular Biology 2021Quote: ... siRNAs were transfected on day 1 and 3 with LipofectamineTM RNAiMAX (Thermo Fisher) and collected on day 8 for analysis ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then treated with hexamethyldisilazane-trimethylchlorosilane-pyridine solution (3:1:9; ThermoFisher) for 20 min at 110°C ...
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bioRxiv - Physiology 2020Quote: ... 1 h with oxygenated water 3% (Tyramide Superboost Kit, B40922, Thermo Fisher, USA) and ...