Labshake search
Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... the cells at 90% confluency were transduced using CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific). The media was changed 24 h after transduction and then daily ...
-
bioRxiv - Cell Biology 2020Quote: ... was used to transfect HEK-293 cells at 70-90% confluency using Lipofectamine 3000 reagent (Thermo Fisher Scientific) as per manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... Cell pellets were resuspended in 1 mL pre-chilled freezing medium containing 90% Fetal Bovine Serum (FBS, Gibco) and 10% DMSO (Sigma) ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were grown to 90% confluency on a T75 flask using DMEM media with 10% FBS (ThermoFisher) at 37 °C and 5% CO2 ...
-
bioRxiv - Pathology 2021Quote: ... Hepatocytes were allowed to attach for 90 minutes on collagen-coated plates in RPMI containing 10% FBS (Gibco), followed by overnight starvation in serum-free medium before experiments (Lipogenesis and β-oxidation assay).
-
bioRxiv - Bioengineering 2021Quote: ... If the confluency reached around 80-90 % the HUVECs were detached with TrypLE™ Express (Gibco, 12605-028) and partially re-plated in expansion medium or used for the 3D culture ...
-
bioRxiv - Neuroscience 2021Quote: ... 90 nl of CTb 488 and 180 nl of Alexa 647-conjugated CTb (CTb 647; Thermo Fisher Scientific) were injected into the GPe (in mm ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells expressing EC-BioID and C-BioID were seeded on p150 dishes and cultured to 70%-90% confluency in Dulbecco’s Modified Eagle’s Medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (Gibco) ...
-
bioRxiv - Immunology 2020Quote: Transfections were carried out in 293T cells seeded at 70-90% cell density using Lipofectamine 3000 (ThermoFisher Scientific) as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... transiently transfected at an 80–90 % confluence using a Lipofectamine® 3000 Transfection Kit (Invitrogen, Thermo Fisher Scientific). Each well received 100 ng of the pMIR-REPORT™ Luciferase vector containing the 3’UTR-hTLR4 (without a negative control ...
-
bioRxiv - Cell Biology 2021Quote: ... transiently transfected at an 80–90 % confluence using a Lipofectamine® 3000 Transfection Kit (Invitrogen, Thermo Fisher Scientific). Each well received 100 ng of the pMIR-REPORT™ Luciferase vector containing the 3’UTR-hTLR4 (without a negative control ...
-
bioRxiv - Neuroscience 2021Quote: ... The brains were then mounted on frosted and 90° ground object slides (AGAA000001##12E, ThermoFisher Scientific, MA, USA) inside paper hole reinforcements (3510 ...
-
bioRxiv - Genomics 2023Quote: RNA was extracted from cells grown to 80-90% confluence using the PureLink RNA mini kit (Invitrogen #12183018A). Samples were processed for stranded total RNAseq libraries ...
-
bioRxiv - Systems Biology 2023Quote: ... Human embryonic kidney cells (HEK293T) were cultured in 10% DMEM (90% DMEM high glucose with GlutaMAX (Gibco, 10569010), 10% FBS (GE ...
-
bioRxiv - Plant Biology 2023Quote: ... siliques of st 17 flowers from T3 generation plants were fixed in acetone 90% (v/v) (Thermo Fisher) at -20 °C overnight (ON) ...
-
bioRxiv - Neuroscience 2023Quote: ... 90% acetonitrile in water) was employed for peptide separation on an ASY-nLC 1200 UPLC system (ThermoFisher Scientific). The separation gradient was set as follows ...
-
bioRxiv - Cell Biology 2023Quote: ... All stocks of hESC lines are preserved in liquid nitrogen in 90 % Knock-out serum (Thermo Fisher Scientific) and 10% sterile DMSO ...
-
bioRxiv - Biochemistry 2023Quote: ... The coverslips were washed and incubated for 90 min with AlexaFluor dye- conjugated secondary antibodies (Thermo Fisher Scientific). Afterward ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell stocks were previously frozen at 1 million cells/ml in 90% fetal bovine serum (FBS, ThermoFisher #SH300880340) and 10% DMSO ...
-
bioRxiv - Cell Biology 2022Quote: ... Human primary WI-38 and IMR-90 diploid fibroblasts (Coriell Institute, AG06814-J, I90-79) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FBS ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were fed twice a week and passaged when they reached 90% confluence using trypsin EDTA (ThermoFisher Scientific). Cultured cells were used at passage 3 or 4 ...
-
bioRxiv - Developmental Biology 2023Quote: ... At 90% confluence cell transfection was performed using Opti-MEM™ Reduced Serum Medium (Thermo Fisher CN:31985062) with PGL2 ...
-
bioRxiv - Cell Biology 2023Quote: ... HUVECs were cultured in complete medium supplemented with 90% Dulbecco’s modified Eagle’s medium (DMEM; C11995500CP, Gibco, United States), 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2023Quote: ... Arabinose was made as a 20% stock solution in 10:90 and filtered through 0.2μm filters (Fisher Scientific) before diluting into 10:90 for growth assays.
-
bioRxiv - Genomics 2024Quote: 90 µL of supernatant from liquid cultures of passaged colonies were mixed with 10 µL chloroform (Fisher Scientific), centrifuged for 1 minute at 21,000 RCF at 25° C ...
-
bioRxiv - Biophysics 2024Quote: ... To prepare cell lysate cells were subjected to sonication for 90 seconds (pulse 15, Amp1 60%) (Fisher Scientific) and then pelleted by centrifugation at 16000 RCF for 20 minutes at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... and the coated plates were blocked with 90 µl 10% SuperBlock™ T20 (PBS) Blocking Buffer (ThermoFisher, 37516) in PBS at 500x rpm for 3 hours at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... 10 µl bacterial suspension was added to 50,000 hMDM or THP-1 cells (D.O.I. = 10) in 90 µl Opti-MEM reduced serum media (Thermo Fisher) and plates were centrifuged for 5 minutes at 500 x g to promote contact ...
-
bioRxiv - Bioengineering 2024Quote: ... the MDA-MB-231 cells were harvested after reaching 90% confluence with 0.25% trypsin-EDTA (25200-072, Gibco) and resuspended in the culture medium at a concentration of 5.0 × 107 cells/mL ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Gels were run at constant voltage (100 V) for 90’ in a Mini Gel Tank (Invitrogen, Waltham, MA) and afterwards stained for 1 h in 50 ml of 0.5X TBE buffer supplemented with 5 µl of SYBR Gold dye (Invitrogen ...
-
bioRxiv - Physiology 2024Quote: ... ADSCs were grown to 90% confluence and differentiated into adipocytes using a StemPro™ Adipogenesis Differentiation Kit (Gibco) following the manufacturer’s instructions.
-
Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3XbioRxiv - Neuroscience 2024Quote: ... and 90 min after plating the medium was replaced with pre-warmed Neurobasal Plus (Thermo Fisher Scientific, #A3582901) supplemented with B27 Plus (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were passaged at 70-90% confluency with 75 μL/cm2 0.5 mM EDTA (Thermo Fisher Scientific, #15575020) for 5-7 min ...
-
bioRxiv - Molecular Biology 2024Quote: Cells were grown to 80-90% confluency and crosslinked with 0.5 mg/mL disuccinimidyl glutarate (DSG; Thermo Fisher) in PBS for 45 min at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... once in 500 µL Wash Buffer (90) and then resuspended in 1X NuPAGE Sample Loading Buffer (Invitrogen, NP0007) and 1X NuPAGE Reducing Agent (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then rocked for 90 minutes at 4°C with dissociation buffer containing 3mM EDTA (Invitrogen; Cat# AM9260G) and 0.5mM DTT (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 µg of total RNA were reverse transcribed with High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) following manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... Ileal H&E sections were then imaged at 10X with an EVOS FL Auto 2 Cell Imaging System (Invitrogen, CA) to measure extent of ileal involvement of each NEC Grade ...
-
bioRxiv - Genetics 2021Quote: ... animals were resuspended in 200 µl of M9 and 1 µl of 2 mg/ml DiI (Molecular Probes, Carlsbad, CA) in dimethylformamide (DMF ...
-
bioRxiv - Microbiology 2021Quote: ... 5 × 106 HEK 293T cells were co-transfected with pNL4-3.Luc.R-E- and recombinant SARS-CoV-2 Spike (D614G) plasmid or its derivatives (B.1.1.7 and B.1.617) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Supernatants containing pseudotyped viruses were harvested 48 h post-transfection ...
-
bioRxiv - Cell Biology 2021Quote: iPSC-CMs cultured in chamber slides were loaded with 1 μM Rhod-2 (Molecular Probes, Life Technologies, Carlsbad, CA, USA) in Hank’s balanced salts modified buffer (HBSS ...
-
bioRxiv - Cell Biology 2021Quote: iPSC-CMs cultured in chamber slides were loaded with 1 μM Rhod-2 (Molecular Probes, Life Technologies, Carlsbad, CA, USA) in Hank’s balanced salts modified buffer (HBSS ...
-
bioRxiv - Molecular Biology 2020Quote: ... sections were then incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse AlexaFluor 568 (Invitrogen, Carlsbad, CA) at 1:500 dilution ...
-
bioRxiv - Physiology 2020Quote: ... We used 2 µg RNA for reverse transcription with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA, USA). The cDNA was diluted (1:10 ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were counterstained with DAPI (0.25 μg/ml) for 2 min and embedded in Prolong Gold (Invitrogen, Carlsbad, CA, USA). When staining for α-tubulin and actin ...
-
bioRxiv - Microbiology 2024Quote: Phage DNA was isolated by treating 2 ml of Phage solution with 1 U/μL DNAse (Invitrogen, Carlsbad, CA, USA) to remove free DNA ...
-
bioRxiv - Cell Biology 2023Quote: ... Tissues from Ca1/2-Ca12/13 from each mouse were pooled and stored into RNAlater Reagent (Invitrogen, Carlsbad, CA, USA) at -80°C till isolation (n = 6 mice/genotype) ...
-
bioRxiv - Genetics 2023Quote: ... 2–5 μg of total RNA was reverse transcribed using a Superscript First-Strand Synthesis system (Invitrogen, Carlsbad, CA, USA). The quantitative RT-PCR was performed using the suh1-4 RT primers (Supplementary Table S2 ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mM L-glutamine and 100 U/ml penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA, Thermo Fisher Scientific, Waltham, MA, USA). The normal human lung cell line HSAEC1-KT (ATCC® CRL-4050™ ...
-
bioRxiv - Cell Biology 2024Quote: ... For the Ca+2 washout experiments Cells were grown for 24 hours and then the growth medium was replaced to medium without Ca+2 (DMEM-Gibco) for a different periods of time ...