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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... 50μL of the supernatant was transferred into a new 96-well plate (Fisher Scientific: 07-200-90) to measure lactate dehydrogenase (LDH ...
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bioRxiv - Microbiology 2024Quote: ... 90 µg of total protein lysate were loaded onto SDS-PAGE 4-12% Bis-Tris gels (Invitrogen); an Ab rabbit anti-CPSF6 (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... 90 μL aliquots of the supernatant were transferred into a black clear bottom 96-well plate (Nunc) and the GFP fluorescence (excitation 485 nm ...
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bioRxiv - Neuroscience 2024Quote: ... The SMART RT reaction was conducted at 42°C for 90 minutes using commercial SuperScript II (Invitrogen) and a Template-Switching Oligo ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were grown to 80–90% confluency and then passaged as clusters using Versene (Thermo Scientific, USA) into 6-well plates for hiPSC maintenance and into 24-well plates for hiPSC differentiation ...
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bioRxiv - Cancer Biology 2024Quote: ... After 90 min incubation at 37 °C cells were washed using advanced DMEM/F-12 medium (Gibco). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... for 90 min at 30°C and derivatized with MSTFA with 1% TMCS (Thermo Fisher, TS-48915) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: Figure 2: Total RNA was isolated from cells or embryos using Trizol (Ambion by Life Technologies, Carlsbad CA, 15596018) followed by extraction using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... whole menisci were cut into small pieces (1-2 mm) and digested in DMEM (11996-065, Gibco, Carlsbad, CA) containing 2% penicillin/streptomycin (15140-122 ...
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bioRxiv - Neuroscience 2020Quote: ... cell membranes were loaded with the voltage-sensitive dye D-2- ANEPEQ (JPW1114, 0.2 mg/mL, Invitrogen, Carlsbad, CA) for 30 minutes using a first patch clamp recording and then re-patched a second time with dye free solution as previously described (Canepari et al ...
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bioRxiv - Microbiology 2021Quote: ... Each 20-μlL PCR mixture contained 2 μL of 10X Accuprime PCR buffer II (Life Technologies, CA, 1 USA), 0.15 μL of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Striatal pieces were then transferred to 10 ml sterilin tubes containing 3ml of HA-Ca + 25 mM L-Glutamine medium supplemented with 2 mg/mL papaïn (Whortington) and 25 mg/mL dispase (Gibco) and incubated for 30 minutes at 37°C with shaking (200 rpm) ...
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bioRxiv - Cell Biology 2020Quote: ... Hoechst 33342 stain (2 mg/ml) and wheat germ agglutinin (WGA) Alexa Fluor 594 conjugate (#W11262, Invitrogen, CA, USA) were added for 10 min in PBS before the slides were mounted ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μg of RNA was incubated at room temperature for 15 min with DNase I (Invitrogen, Carlsbad, CA, USA). The product was incubated with random hexamer primers ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The tumor was excised and was washed in homogenization buffer (0.25 M sucrose [Merck, CAS 57–50–1], 10 mM HEPES, 1 mM EDTA, 2% antibiotic and antimycotic [ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by an enzyme solution consisting of 2 nM Cel7A labeled with 0.5 nM Qdot (Thermo Scientific; Cas: Q10143MP), 5 mM dithiothreitol ...
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bioRxiv - Immunology 2024Quote: ... murine BMDCs at 2 × 106 cells/mL were stained with 5 μM carboxy-H2DCFDA (Invitrogen™, Carlsbad, CA, USA) and pre-incubated with prepared fatty acids (1 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... Following that cells were loaded (40 min at 37°C) with 2 µM Fura-2AM (Invitrogen; Carlsbad, CA, USA), for ratiometric cytosolic Ca2+ [Ca2+]c measurements ...
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bioRxiv - Microbiology 2024Quote: ... cells were mounted using Prolong gold anti-fade reagent containing 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, Carlsbad, CA). Images were captured using a Leica SP8 laser scanning confocal microscope (Leica Microsystems ...
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bioRxiv - Cell Biology 2024Quote: ... were injected into an Acclaim PepMap100 Nano-Trap Column (100 μm × 2 cm, ThermoFisher Scientific, San Jose, CA, USA) containing 5 μm C18 particles at a flow rate of 5 μl per min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lot 183859), ammonium formate (99%, AC401152500), acetonitrile (ACN; Optima®, Lot 184819), and formic acid (99.5%, A117-50) from Fisher Scientific (Waltham, MA) was used for extractions and instrument solvent gradient ...
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bioRxiv - Molecular Biology 2021Quote: ... containing varying concentrations of the corresponding crowding agent: 0/5/10/305515% w/v of serine (L-Serine, 99%, ACROS Organics™, AC132660250), 0/5/10/15% w/v of Polyethylene glycol 3350 (PEG ...
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bioRxiv - Neuroscience 2023Quote: Fly brains expressing Atg8-mcherry were dissected in Schneinder’s media and stained for 3 minutes with lysotracker (LysoTracker Red DND-99; Thermo Fisher Scientific; 1:2000), rinsed in Schneinder’s media and then directly visualised with a Zeiss Airyscan 880 ...
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bioRxiv - Neuroscience 2024Quote: ... brains were dissected in phosphate-buffered saline (PBS) followed by 15 minutes of incubation with 200 nM LysoTracker™ Red DND-99 Red probe (Thermofisher Scientific, L7528) in the dark ...
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bioRxiv - Molecular Biology 2024Quote: ... cerevisiae purified 40 nm VLPs using randomly selected primer from laboratory stock 99.R and RevertAid H Minus Reverse Transcriptase (Thermo Fisher Scientific, USA) performing cDNA synthesis step at 37 °C ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: Mice were anaesthetised using 1mg/kg Medetomidine Hydrochloride (Dormitor) and 75mg/kg ketamine (Ketavet) in 0.9% saline (Thermo Fisher Scientific). Mice were irradiated using a single 137Cs source in a Shepherd Mark-I-68A 137Cs irradiator (JL Shepherd & Associates) ...
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bioRxiv - Immunology 2020Quote: ... 25 μl/well of 60 mM water solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Thermo Fisher Scientific) were added ...
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bioRxiv - Neuroscience 2023Quote: Cocaine hydrochloride and morphine were provided by the National Institute on Drug Abuse (NIDA) and dissolved with 0.9% saline (Fisher Scientific). Mice were injected intraperitoneally (i.p. ...
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bioRxiv - Bioengineering 2024Quote: ... 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Cat. A638729) were purchased from Aladdin (Shanghai). KLH (Cat. 77600) was purchased from ThermoFisher. Peptide synthesis was conducted by Genscript (Nanjing ...
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Tuning non-linear mechanics in collagen hydrogels modulates cellular morphotypes in three dimensionsbioRxiv - Biophysics 2024Quote: ... expressing mScarlet-I H2B42 and TIFs expressing Paxillin-mCherry was established by lentiviral transduction.54,79 TIF cells were cultured in 10 µg/mL Blasticidin S Hydrochloride (Gibco, A1113903).
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bioRxiv - Neuroscience 2023Quote: ... The carboxylic groups on the carbon fiber surface were electro-activated by incubation in 0.4 M 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC; Life Technologies) and 0.1 M and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Cancer Biology 2024Quote: Cells were minced and lysed in lysis buffer (8 M urea, 100 mM Tris hydrochloride, pH 8.0) containing protease and phosphatase inhibitors (Thermo Scientific) followed by 1 min of sonication (3 s on and 3 s off pulse ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked in CAS block (Invitrogen, Carlsbad, CA) and incubated in primary antibodies at 4°C overnight (for dilutions ...
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bioRxiv - Developmental Biology 2021Quote: ... and the stroma was resuspended in 90 µL FACS buffer ((1xHank’s buffered saline solution (HBSS) (Life Technologies #14175095), 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... the remaining 90% was incubated with anti-ThPOK antibody bound to goat anti-rabbit M280 magnetic beads (Invitrogen) overnight at 4°C prior to washing with RIPA buffer and overnight decrosslinking at 65°C ...
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bioRxiv - Developmental Biology 2021Quote: ... except that animals were permeabilized with 5 µg/ml Proteinase-K for 90 seconds (ThermoFisher Scientific, cat# AM2548), and hybridized with 1ng/µl of RNA probe for a minimum of 48 hours at 62°C.
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bioRxiv - Neuroscience 2021Quote: ... confluent cultures (90%) were treated overnight with siRNA Px1 mixed with 10 μl/ml Lipofectamine reagent (ThermoFisher Scientific). Transfection reagents were removed and cells incubated for 4-6 h in DMEM medium (2% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... while 90% was incubated with RQ1 DNAse for 30 min for later RNA extraction with Trizol LS (Ambion).
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bioRxiv - Molecular Biology 2021Quote: ... 10% of beads were set aside for quality control and 90% resuspended in 1ml Trizol (Thermo Fisher Scientific) and stored at -80°C until further processing.
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bioRxiv - Neuroscience 2020Quote: ... Ps2Ex3 sgRNA (90 ng/μL final concentration) was first mixed with Cas9 nuclease (Invitrogen, Carlsbad, California, USA, B25640), and then incubated at 37°C for 15 min to maximize cleavage efficiency ...
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bioRxiv - Microbiology 2021Quote: ... The coverslips were stained with DAPI for 90 sec and mounted with 15-20 μl Antifade (Invitrogen, USA) to reduce photo-bleaching ...
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bioRxiv - Cell Biology 2021Quote: ... RNA transfection experiments were performed in NHF-1 and IMR-90 cells for 48 hours using RNAiMAX (ThermoFisher) transfection reagent according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... and the rest of the excreta (90 μl) was added to 110 µl Glucose Reagent (Thermo Scientific, #TR15421) and incubated for 3 minutes at 37°C for glucose level measurement ...
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bioRxiv - Molecular Biology 2022Quote: ... 90 µM β-mercaptoethanol (MilliporeSigma, Cat# M3148) and 100 U/mL penicillin-streptomycin (Thermo Fisher Scientific, Cat# 15140122). INS-1E cells were cultured in 11 mM RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... run at 150 V for 90 minutes in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific). Proteins were electrotransferred onto an Immobilon-fl polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2022Quote: ... Samples were then diluted 8x with S-Trap buffer (90% methanol, 100 mM triethylammonium bicarbonate (TEAB) (ThermoFisher, 90114)) solution ...
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bioRxiv - Bioengineering 2022Quote: ... [28] Upon reaching ≥ 90 % confluence (day 0) media was exchanged to RPMI 1640 medium (RPMI; 1185063, Gibco, USA) supplemented with B27 supplement without insulin (B27-I ...