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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... Barcoded samples were then pooled and size-selected on a 2% agarose Invitrogen E-gel (Invitrogen, Carlsbad, CA) for fragments ranging between 350-450 bp in size ...
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bioRxiv - Cell Biology 2020Quote: ... washed with organoid media and then incubated with 100 cell pellet volumes of organoid media with 10 nM LysoTracker Red DND-99 (Thermo Fisher L7528) at 37C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... Grids were blotted from the backside for 10 seconds at 4℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific). We removed one of the blotting arms for one-side blotting because Birbeck granules would be absorbed onto the filter paper ...
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bioRxiv - Biophysics 2022Quote: Lysosomes were stained by loading the cells with LysoTracker™ Red DND-99 (1 mM, ref. L7528) at a final concentration of 50 nM purchased from Life Technologies | Thermo Fisher Scientific (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... the plate was again washed six times and 100 μL/well substrate (200 μL 40 mg/mL o-Phenylenediamine dihydrochloride 99+% (OPD, Acros Organics BVBA) + 2 μL H2O2 (Merck ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were heated at 99 °C for 5 min and separated using Novex Precast 4–20% gels (Invitrogen, Waltham, MA, #WXP42020BOX) in SDS-PAGE ...
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bioRxiv - Systems Biology 2023Quote: ... and 99%) were used to dehydrate the slides and a coverslip was mounted with Diamond anti-fade mounting medium (Invitrogen, cat.no. P36961).
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bioRxiv - Cell Biology 2023Quote: BMDMs were seeded in 96 well plates (6055302, Revvity) and stained with 25 nM LysoTracker DND-99 (LTR) (L7528, Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Dodecane overlay cultivations were performed under the same conditions as controls with no extraction aid but with the addition of 20% of the cultivation volume of sterile dodecane at 99% purity (Acros Organics, UK). All microplate cultivations were made in triplicates to increase the statistical robustness of the results ...
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bioRxiv - Genomics 2023Quote: Adults were relaxed by incubating for 3–16 hours at 4 °C in about 400 mL ASW with approximately 5 g of 99% L-menthol crystals (Thermo Scientific A10474) (3 adults per 400 mL volume) ...
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bioRxiv - Genetics 2024Quote: ... Samples were denatured at 99°C for 5 minutes and separated on a 4-20% tris-glycine protein gel (Thermo Fisher Scientific) at 150 V for 75 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Media was aspirated and replaced with 100 μL fresh full serum DMEM media supplemented with 15 μM LysoLive GalGreen fluorogenic substrate (MarkerGene Technologies, M2776) and 50 nM LysoTracker Red DND-99 (Thermo Scientific, L7528) and incubated at 37°C for 2 h ...
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bioRxiv - Genomics 2024Quote: ... diluted 1:100 or indirectly using a rabbit polyclonal antibody (99) (1:10 dilution) in conjunction with a goat anti-rabbit conjugated secondary (Jackson ImmunoResearch 111-295-003 or Invitrogen A11008) diluted 1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were heated with an increasing gradient of 0.05°C/s from 25 to 99°C and melting curves were recorded using a QuantStudio 5 Real-Time PCR System system (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... and the resulting gel was digested in 1 mL of digestion buffer (0.8M guanidine hydrochloride and 0.5% Triton X-100 in 1X TAE buffer) by Proteinase K (0.2mg/mL, Thermofisher, AM2548) for 1 hour at 45°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and a protease inhibitor cocktail containing Leupeptin (Carl Roth, CN33.4), PMSF (Carl Roth, 6367.3), Pepstatin A (Carl Roth, 2936.3) and Benzamide hydrochloride (Acros Organics, E1014) for 1 h at 4°C on a rotator ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
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bioRxiv - Cell Biology 2020Quote: ... iPSCs at 90% confluence were incubated with differentiation basal medium comprising RPMI 1640 medium (Thermo Fisher, 11875095) and B27 supplement minus insulin (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: The plasmid library was transfected in triplicates to 90 million cells/replicate using Neon transfection system (Invitrogen). The cells were detached with trypsin ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... immature peripheral neurons were frozen in 90% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA) and 10% dimethyl sulfoxide (DMSO) ...
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bioRxiv - Microbiology 2022Quote: ... and iSLK.219 cells (a generous gift from Don Ganem) (90) were cultured in DMEM (Thermo Fisher) supplemented with 100 U/mL penicillin ...
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bioRxiv - Molecular Biology 2021Quote: ... and mixed with 90 µl 1x RNA Sequencing Buffer from the RNase T1 kit (Thermo Fisher, AM2283). After incubation at 50°C for 5 min and on ice for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... The microscopy slide was then transferred to a plastic Petri dish (90 mm, Thermo Fisher, Waltham, USA), and five circular agarose pads (Ø 10 mm ...
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bioRxiv - Genomics 2020Quote: More than 90% recovery of ligated RNA and cDNA was achieved from 15% TBE-Urea (Invitrogen EC6885BOX) and 10% TBE-Urea (Invitrogen EC6875BOX) ...
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bioRxiv - Biochemistry 2020Quote: ... 90 μl aliquots of the diluted lysate were then placed into 250 μl PCR tubes (Fisher Scientific). 10 μl of 10X stocks of either CMP or m5CMP (made up in 25 mM HEPES pH 7.5 and 150 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were briefly air-dried and manually mounted using ~90 μl of Prolong Diamond Antifade (Fisher Scientific) and standard coverslips (24 × 50 mm2 ...
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bioRxiv - Cell Biology 2021Quote: ... and washed with trituration media (90% of Dulbecco Modified Eagle Medium and 10% fetal bovine serum, Invitrogen). Dissociated cells were seeded onto 50 μg/ml of poly-D-lysine (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... The cell pellets were resuspended in 90% fetal bovine serum (FBS; 10270106, ThermoFisher Scientific, Waltham, MA, USA) with 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2022Quote: ... followed by ultracentrifugation on the T1270 rotor at ~280,000xg for 90 min at 4°C (Thermo Scientific). After ultracentrifugation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... immature peripheral neurons were frozen in 90% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA) and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were briefly air-dried and manually mounted using ∼90 µl of Prolong Diamond Antifade (Fisher Scientific) and standard coverslips (24 × 50 mm2 ...
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bioRxiv - Cell Biology 2022Quote: hPSCs were cultured to 80-90% confluence and harvested using Cell Dissociation Reagent (Accutase; Thermo Fisher Scientific) for 4-6 min at 37 □ ...
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bioRxiv - Systems Biology 2021Quote: ... 90% for each combined TMT-set was purified on Pierce peptide desalting spin columns (Thermo Fisher Scientific) according to the manufacturer’s instructions and separated into 2 equal aliquots ...
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bioRxiv - Cell Biology 2021Quote: ... PC12 cells were transiently transfected at 90% confluency according to manufacturer’s guidelines with Lipofectamine 2000 (Invitrogen, #11668) with 1.5ug of each DNA construct to be expressed ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were purified to ≥ 90% purity as assessed by SDS-PAGE and PageBlue protein staining (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... medium containing virus was processed by centrifugation (Sorvall WX-90 Ultracentrifuge and SureSpin 630 rotor; ThermoFisher Scientific), using a sorbitol cushion (20% sorbitol ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were dehydrated with alcohols (70%, 90%, 100% Ethanol) and cleared with xylenes (Fisher Scientific, Catalog #X3P). Slices were then placed on glass slides (Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... samples were stained for 90 min with 1.65 x 10−7 M Alexa Fluor 488 phalloidin (Invitrogen, Life Technologies Corporation ...
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bioRxiv - Microbiology 2022Quote: ... samples were denatured at 90°C for 10 min with a 10x reducing agent (Thermo Fisher Scientific) before electrophoresis on 4-12% Bis-tris gels ...
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bioRxiv - Biochemistry 2024Quote: ... heated to 90°C for 5 min and then loaded onto 12% NuPAGE Bis-Tris Gels (Invitrogen). Proteins were separated using NuPAGE MOPS (3-(N-morpholino)propanesulfonic acid ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 90 minutes in anti-Rabbit Alexa Fluor 488 (Invitrogen, 1:500 dilution) to detect Phospho-Tyrosine and anti-Mouse Alexa Fluor 633 (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were then permeabilized by 1 min incubation in EPS solution (90% D[+] Limonene [Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The SMART RT reaction was performed at 42°C for 90□min using commercial SuperScript II (Invitrogen) and a TSO ...
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bioRxiv - Neuroscience 2023Quote: ... NESC were cultured in Geltrex-coated (1:90 dilution in media, Thermo Fisher Scientific, cat. no A1413302) 6-well plates in maintenance media (base culture media further supplemented with 150 µM ascorbic acid (AA ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... 90% Percoll was diluted in L15 complete media [Leibovitz’s L-15 Medium without phenol red (Gibco, 21083027) + 10% calf bovine serum (ATCC 50-189-025NP ...
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bioRxiv - Genetics 2022Quote: The template RNA was extracted by homogenizing 80-90 male flies suspended in TRIzol™ (ThermoFisher, #15596026) in a Mini-Beadbeater-16 (BioSpec ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were denatured at 90 °C for 10 min with 10× reducing agent (Thermo Fisher Scientific) before gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... at 90% confluency (24 pmol siRNA per 12 well dish cavity with Lipofectamine RNAiMAX, Thermo Fisher Scientific). Sixty-five hours later ...
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bioRxiv - Microbiology 2024Quote: Cryo-EM FIB-milled grids were rotated by 90° and loaded into a Titan Krios microscope (Thermofisher) equipped with a K3 direct electron detector and (Bio- ...
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bioRxiv - Developmental Biology 2024Quote: ... Phoenix-Eco cells were cultured at 37℃ in 5% CO2 with standard culture medium [90% DMEM (Gibco), 10% Fetal Bovine Serum (Corning) ...