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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... cells at approximately 90% confluence were detached with TrypLE cell dissociation reagent (Gibco 12605-036), resuspended in a calcium-free alginate diluent buffer (145 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... free fatty acids were extracted from cells using 90% methanol (LC-MS grade, Thermo Scientific). The extracts were clarified by centrifugation at 15,000 x g for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli was incubated at 90°C in digital dry baths/block heaters (Thermo Fisher Scientific) for 10 min and placed on ice for 3 min before being transferred to the sensor culture containing 1 mL LB ...
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bioRxiv - Neuroscience 2024Quote: ... iPSC colonies grown till 90% confluency were dissociated using 1 mM EDTA (Invitrogen, 15575-038) in 1X PBS (VWR ...
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bioRxiv - Bioengineering 2024Quote: ... and HUVECs were harvested after reaching 90% confluence with 0.05% trypsin-EDTA (25300-062, Gibco) and resuspended in the culture medium at a concentration of 5 × 108 cells/mL ...
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bioRxiv - Immunology 2024Quote: ... frozen in 5 × 106 cells/ml aliquots in 90% FBS/10% DMSO (Thermo Fisher Scientific) and stored at –80°C prior to being shipped on dry ice to New York ...
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bioRxiv - Biophysics 2024Quote: ... Cells were routinely subcultured at ∼90% confluency using 0.25% trypsin-0.53 mM EDTA (Thermo Fisher). For primary rat astrocytes DMEM supplemented with 20% FBS was used for culturing and accutase (Thermo Fisher ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were trypsinized at 90% confluency by using 0.25 % trypsin ethylenediaminetetraacetic acid (EDTA; Invitrogen). HBMSCs were further cultured to passage 3–5 ...
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bioRxiv - Cancer Biology 2024Quote: Free fatty acids were extracted from cells using 90% methanol (LC/MS grade, Thermo Scientific). The extracts were clarified by centrifugation and dried down using a SpeedVac ...
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bioRxiv - Plant Biology 2020Quote: DNA fragment lengths were determined on an ABI 3500xl Genetic Analyzer from 1 μL of 1:99 diluted PCR products mixed with 8.9 μL of HiDi Formamide (Life Technologies), and 0.1 μL of Gene Scan 500 LIZ Size Standard (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The N- and C-terminus of thyA (lpg2868) were amplified by BKMP94-95 and BKMP98-99 primers and introduced into pDonorP1-P4 and pDonorP3-P2 (Invitrogen) to generate pMME1084 and pMME1094 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were heated to 99 °C for 15 min and aliquots (5 µL) resolved by SDS-PAGE on 8 % – 16 % Tris-glycine minigels (Invitrogen) (150 V ...
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bioRxiv - Immunology 2021Quote: ... The RNA was then dissolved in a 10μL mixture of 99 μL of DNase/RNase/Nucleotide-free water and 1 μL of SUPERase•In™ (Invitrogen, Life Technologies Incorporated ...
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bioRxiv - Molecular Biology 2023Quote: ... immediately followed by a triple MS2 stem-linker sequence (PNAS September 17, 2002 99 (19) 12203-12207) was purchased from ThermoFisher. This was used as a template to make pcDNA3.0_NORAD_NRU78_MS2 ...
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bioRxiv - Biochemistry 2024Quote: ... at 100 µg/mL in complete DMEM medium for 4 h and treated with LysoTracker Red DND-99 (Invitrogen #L7528) for 15 min and subsequently imaged on a Nikon CSU-W1 SoRa microscope ...
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bioRxiv - Developmental Biology 2023Quote: ... Lens apoptosis was determined in the same SAG and control DMSO embryos as heart eye measurements were made by staining with 5 μg/ml Lysotracker Red DND 99 (Invitrogen) for 30 min in the dark as described previously (Ma et al. ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... 24h after plating were incubated for 2h in LysoTracker Red DND-99 (75 nM) and for 45 min in Hoechst 33342 (Invitrogen) (0.25μg/ml).
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bioRxiv - Biophysics 2022Quote: ... 0.020 M potassium phosphate monobasic), sodium chloride (BioXtra grade, purity ≥99.5%) and D-(-)-Fructose (HPLC grade, purity ≥99%) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated under heating from 25 °C to 99 °C in StepOne Plus Real-time PCR system (Thermo Fisher Scientific). The kinetics of fluorescent signals were analyzed to calculate Tm values in Protein Thermal Shift Software 1.3 (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2022Quote: ... The sections were then cleared and stained in Clearsee solution with Basic Fuchsin (Fisher scientific Cat no 632-99-5) for 30 minutes followed by two washing steps in Clearsee only ...
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bioRxiv - Cancer Biology 2023Quote: ... The measurement of lysosomal pH and Cathepsin B activity was evaluated with LysoTrackerTM Red DND-99 probe (InvitrogenTM, ThermoFisher Scientific) and the Magic Red® kit (Immunochemistry Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Phosphopeptide enrichment was carried out on the supernatant as described in 99 using the KingFisher Apex robot (Thermo Fisher Scientific) with 25 µl of Fe-NTA MagBeads (PureCube ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondly a PCR was performed using the same primer 99.R (Table 3) and Taq polymerase (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed for 2 genes of interest and 2 internal controls (Suppl Figure S1) using Taqman® gene expression assays (Thermo Fisher, Foster City CA) and Taqman® Fast Universal Master Mix (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... The 2-mL PCR reactions used the Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific, Carlsbad, CA) and the forward primer 5’-GCAGAATTCTAATACGACTCACTATAGGTTGCCGAATCC-3’ ...
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bioRxiv - Genetics 2020Quote: ... 10ng DNA was used as template for 2 µl TaqMan assays (Applied Biosystems, Foster City, CA, USA) performed on the ABI 7900HT platform in 384-well format and analysed with Autocaller software ...
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bioRxiv - Neuroscience 2021Quote: ... loaded into 12% SDS-PAGE gels and transferred to nitrocellulose membranes using iBlot 2 (Invitrogen, CA, USA). Membranes were incubated in blocking solution containing 5% skim milk (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... and washed twice with 2 ml 1× Dulbecco’s phosphate-buffered saline (DPBS) (Gibco, 14190136, Carlsbad, CA, USA) solution containing 2% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in VeroE6 cells in Opti-MEM I (Invitrogen, Carlsbad, CA, USA) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... VeroE6 and MDCK ⍺2-6 cells were cultured in minimum essential medium (MEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2024Quote: ... An aliquot of 2 mM stock solution in DMSO of FM4-64 (Cat # 13320, Invitrogen, Carlsbad, CA) was made and stored at -20°C ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription was performed using 2 μg of DNase treated RNA and Superscript II (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2 g wet weight was homogenized in a TRIzol reagent (Invitrogen, Life Technologies, Carlsbad, CA, USA) by using a bead beater (Biospec ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2 g wet weight was homogenized in a TRIzol reagent (Invitrogen, Life Technologies, Carlsbad, CA, USA) by using a bead beater (Biospec ...
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bioRxiv - Physiology 2024Quote: ... Fluorescently conjugated secondary antibodies were applied in 1:2 primary/secondary molar ratios (Life Technologies, Carlsbad, CA). Labeled samples were incubated in EasyIndex (LifeCanvas Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... coverslipped with ProLong® Diamond Antifade Mountant with diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, CA, USA), and allowed to set for at least 24 hours before imaging.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 2 µg was used to synthesize the cDNA using SuperScript IV Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Gibco 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) was obtained from Invitrogen (Carlsbad, CA) and sterile filtered before use ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2024Quote: ... EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) and Sulfo-NHS (N-hydroxysulfosuccinimide) were purchased from ThermoFisher (USA). Unless otherwise stated all other chemicals were received from Sigma (St Louis ...
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bioRxiv - Biophysics 2021Quote: ... and biotin-PEG-SVA at a ratio of 99:1 (w/w) (Laysan Bio) in 0.1 M sodium bicarbonate (Thermo Fisher Scientific) for 3 h.10 Excess PEG was rinsed with water ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes in live-cell imaging medium and lysosomes were visualized by staining cells with 50nM Lysotracker Red DND-99 (Thermo Fisher) for 30 minutes in live-cell imaging medium according to manufacturer’s description ...
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bioRxiv - Cell Biology 2020Quote: ... Live glands requiring staining were treated for 5 min in a 1:100 dilution of LysoTracker Red DND-99 (Life Technologies) in ice-cold PBS.
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bioRxiv - Neuroscience 2024Quote: For the lysosomal functionality assay fibroblast cells were incubated with CM containing 100 nM LysoTracker™ Red DND-99 (Thermo Fisher) for 5h ...
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bioRxiv - Cancer Biology 2024Quote: ... LMD samples were incubated at 99 °C for 30 min followed by 50 °C for 10 min with SMART trypsin (ThermoFisher Scientific). SMART trypsin was added at a ratio of 1 μg per 30 mm2 tissue ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated for 5 min at 99°C before loading on 4-12 % SDS-PAGE with MOPS as running buffer (Thermo-Fisher Life Technologies). BenchMark protein ladder was used as marker (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: Acidic vesicles were visualised by incubating RAW 264.7 cells in medium enriched with LysoTracker™ Red DND-99 (1:2500) (Invitrogen, L7528) for at least 1 hour prior to fixation at 37°C [35] ...
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bioRxiv - Cell Biology 2022Quote: Lysosomal mass was estimated by incubating cells under various genetic perturbations (7 days after sgRNA transduction) with LysoTracker Red DND-99 at 100 nM (Invitrogen; L7528). After incubation at 37ºC for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... the gut of control or rapamycin-treated flies were dissected out in 4°C chilled PBS and then stained in a 10 μM Lysotracker Red:1μg/ml Hoechst solution (LysoTracker™ Red DND-99, Hoechst 33342 Solution, Thermofisher Scientific) for 3 minutes ...