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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) and Sulfo-NHS (N-hydroxysulfosuccinimide) were purchased from ThermoFisher (USA). Unless otherwise stated all other chemicals were received from Sigma (St Louis ...
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bioRxiv - Molecular Biology 2020Quote: ... was heated at 90 °C for 20 sec with gel loading buffer II (Thermo Fisher Scientific) and placed on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Once 70-90% confluent they were passaged as clumps using EDTA (0.5 mM, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: BSRT7/5 cells at 90% confluence in 48-well dishes were transfected using Lipofectamine 2000 (Invitrogen) with a plasmid mixture containing 0.125 μg of pGaussia/Firefly minigenome ...
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bioRxiv - Biochemistry 2021Quote: ... The chemically synthesized p53 TAD2 domain peptide (38QAMDDLMLSPDDIEQWFTEDPGPD61) was obtained with >90% purity from Thermo Scientific, USA ...
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bioRxiv - Biophysics 2021Quote: ... Movies of 90 frames were acquired during a 9 second exposure on a Krios (ThermoFisher Scientific) microscope with a Quantum-K2 detector ...
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bioRxiv - Biophysics 2020Quote: ... Fusion protein expression plasmids were transfected into (70-90% confluent) cells using Turbofect™ (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... for 90 min at 37 0C and then filtered through a 70-um cell strainer (ThermoFisher) and treated with ACK lysing buffer (Worthington Biochemical) ...
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bioRxiv - Microbiology 2021Quote: ... Viral lysate was diluted 1:10 by adding 90 μl of nuclease-free water (Life Technologies). qRT-PCR reactions were prepared by mixing 9.6 μl of diluted viral lysate with 10.4 μl of reaction mix containing 10 μl of 2x Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation for 90 minutes in streptavidin Alexa Fluor 488 conjugate (1:500; ThermoFisher Scientific). AP signal was visualised by Fast Red staining (Kem En Tec Diagnostics) ...
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bioRxiv - Neuroscience 2020Quote: ... Then, a Neuropixels probe phase 3A (Imec, Belgium) 90 coated with a fluorescent dye (DiD, Thermofisher) was inserted into the brain with the tip reaching down to 1-1.5 mm below the target area ...
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bioRxiv - Molecular Biology 2022Quote: ... and 90 µl 1 µM RBNS RNA library supplemented with 20 units of SUPERaseIn (ThermoFisher Scientific) was added and rotated for 1 hr at RT ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells at ∼80-90% confluency were detached with phosphate-buffered saline (PBS, Gibco® ThermoFisher Scientific) solution supplemented with 1 mM EDTA (Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells at ∼80-90% confluency were detached with phosphate-buffered saline (PBS, Gibco® ThermoFisher Scientific) solution supplemented with 1 mM EDTA (Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged as single cells at 70-90% confluency using TrypLE Express (Thermo Fisher Scientific) and split at a ratio of 1:5 to 1:20 ...
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bioRxiv - Microbiology 2021Quote: ... samples were derivatized with 90 μL of MSTFA with 1% TMCS (Thermo Fisher Scientific, Waltham, MA) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells at ∼80-90% confluency were detached with phosphate buffered saline (PBS, Gibco® ThermoFisher Scientific) solution supplemented with 1 mM EDTA (Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... and the cells were passaged at 90% confluency using Trypsin/EDTA solution (25200072, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... RT4 and 5637 cells were cultured to 80-90 % confluence and harvested using TrypLE Express (Gibco). Cell concentrations were determined with a TC20 Automated Cell Counter (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells at ∼80-90% confluency were detached with phosphate buffered saline (PBS, Gibco® ThermoFisher Scientific) solution supplemented with 1 mM EDTA (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The biopsies were digested by adding 90 µL of proteinase K buffer (4489111, Thermo Fisher Scientific) and 10 µL proteinase K (25530049 ...
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bioRxiv - Microbiology 2022Quote: FIB-milled grids were rotated by 90 degrees and loaded into a Titan Krios microscope (Thermofisher) equipped with a K2 or K3 direct electron detector and (Bio- ...
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bioRxiv - Systems Biology 2022Quote: IMR-90 (human female lung fibroblast; ATCC, CCL-186) cells were grown in DMEM (Gibco, 10564) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... at 40V for 90 minutes using a wet transfer system and NuPAGE transfer buffer (ThermoFisher Scientific) containing 20% methanol ...
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bioRxiv - Microbiology 2024Quote: ... When cells reached 80-90% confluency they were passaged using 0.25% trypsin/EDTA (Gibco, Cat. #25200056).
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bioRxiv - Cell Biology 2024Quote: ... they were grown up to 70-90% confluency and trypsinized for 3 min (Gibco, 15400-054). For injection ...
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bioRxiv - Cell Biology 2024Quote: ... and 90% of samples were dialyzed using 3.5 MWCO Slide-A-Lyzer cassettes (Thermo Scientific; 66330) in RIPA buffer with gentle stirring overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Coverslips were incubated for 45-90 min at 37°C with 1 μM Fura-2AM (Invitrogen) in Neurobasal medium supplemented as above ...
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bioRxiv - Biochemistry 2023Quote: ... 6x106 cells were plated onto standard sterile 90 mm petri dishes (Sterilin™, Thermo Fisher Scientific) with 10 ml of complete RMPI with 1% Penicillin and Streptomycin and 10% L929 supernatant for infection assay experiments ...
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bioRxiv - Microbiology 2023Quote: BSRT7/5 cells at 90% confluence in 96-well dishes were transfected using Lipofectamine 2000 (Invitrogen) with a plasmid mixture containing 62.5 ng of pGaussia/Firefly minigenome ...
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bioRxiv - Cancer Biology 2023Quote: Bulk RNA was extracted from ∼90 mg of snap-frozen liver in 1 mL TRIzol (Invitrogen) and the RNeasyMini Kit (Qiagen) ...
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bioRxiv - Bioengineering 2024Quote: Cryopreserved human primary hepatocytes (post-thaw plateability with >90% viability) were purchased from Thermo Fisher (Hu1960). Hepatocyte donor information is available in supplementary Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were cryopreserved in freezing medium composed of 90% knock-out serum (Thermo Fisher Scientific) and 10% dimethyl sulfoxide (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... Radiolabeled RNA was incubated at 90°C for 5 min with alkaline hydrolysis buffer (OH; Ambion) or at 37°C for 5 min with ribonuclease T1 (0.1 U ...
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bioRxiv - Physiology 2024Quote: ... At 90-100% confluency differentiation was initiated by changing the medium to α-MEM Glutamax (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested at ∼90% confluency and detached with 0.25% Trypsin-EDTA (Thermo Fisher Scientific, UK) and collected by centrifugation at 300 x g for 5 mins ...
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bioRxiv - Cancer Biology 2024Quote: ... for 90 seconds and were mounted in the slides with the mounting media (Invitrogen, Cat #S36938). The slides were visualized using a confocal microscope at 63X (Leica ...
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bioRxiv - Neuroscience 2021Quote: Slices obtained from VGAT-Venus rats were bulk loaded with 50 μM Fura-2,AM (Invitrogen; Carlsbad, CA) 0.2% Pluronic F-127 in our standard extracellular solution but containing 2 mM MgCl2 ...
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bioRxiv - Bioengineering 2022Quote: ... 4-Chlorobenzenesulfonate Salt (DID) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). Ammonium bicarbonate ...
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bioRxiv - Immunology 2020Quote: ... the proliferation capacity of NK92 cells was measured by an EdU (5-ethynyl-2 ‘-deoxyuridine, Invitrogen, CA, USA) proliferation assay ...
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bioRxiv - Molecular Biology 2022Quote: ... After digestion with Type II collagenase (2mg/mL in PBS/2% BSA, Life Technologies Inc., Carlsbad, CA, USA) at 37°C for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... The digestant was diluted into 2% v/v high purity HNO3 (Optima; Fisher Scientific Company; Nepean, ON, CA) in acid-washed 15-mL polypropylene tubes ...
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bioRxiv - Molecular Biology 2021Quote: ... and diluted to 1-2 million cells/mL using a Countess™ Automated cell counter (Invitrogen; Carlsbad, CA). On each day of experiments ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Microbiology 2024Quote: ... Then, 4′,6-diamidino-2-phenylindole (DAPI, Solabio) and Alexa Fluor 488 Conjugate Concanavalin-A (Invitrogen, CA, USA) were added to each slide and incubated for 5 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... hTERT-MSCs were maintained between passage 2 and 15 in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 coding sequences were inserted using the Gateway LR Clonase II Enzyme Mix kit (Invitrogen, CA), yielding plasmids pLCPD-S ...
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bioRxiv - Molecular Biology 2023Quote: ... MMP-2 and −9 activity was determined using gelatine containing zymography gels (Thermo Fisher Scientific, Karlsbad, CA, USA) as previously described (37).
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bioRxiv - Neuroscience 2023Quote: ... we cultivated the cells in a medium with 2% fatty acid-free BSA (Life Technologies Invitrogen, Carlsbad, CA).
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bioRxiv - Neuroscience 2023Quote: ... we cultivated the cells in a medium with 2% fatty acid-free BSA (Life Technologies Invitrogen, Carlsbad, CA).