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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Fly guts were dissected in PBS and stained with lysotracker (LysoTracker Red DND-99; Thermo Fisher Scientific; 1:2000) and Hoeschst 33342 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... VNC were dissected in 1X PBS without fixation and incubated in a lysotracker solution (Lysotracher Red DND-99, Invitrogen) 1:5000 during 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... LysoTracker Red DND-99 was used to label Tg(myo6b:YFP-Hsa.TUBA)idc16Tg larvae while LysoTracker Green DND-26 (ThermoFisher, L7526) was used to label Tg(myo6b:rib a-TagRFP)idc11Tg larvae ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine perchlorate (DiD) (D7757) and LysoTracker Red DND-99 (L7528) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... at 99°C for 10 min and proteins were separated on NuPAGE 4–12% Bis-Tris Gels (ThermoFisher Scientific). They were then transferred onto a PVDF membrane using the iBlot system (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were heated from 25 °C to 99 °C in 1% steps using StepOnePlus (Applied Biosystems, Life Technologies). Data were analyzed using Thermal Shift software (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were heated from 25 °C to 99 °C in 1% steps using StepOnePlus (Applied Biosystems, Life Technologies). Data were analyzed using Thermal Shift software (Life Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... lysosomes were stained with a 500 nM final concentration of LysoTracker™ Red DND-99 (LTR) (L7528, Thermo Fisher) for 20-30 min in serum free medium at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Each PCR mixture contained 2 µl of 10X Accuprime PCR buffer II (Life Technologies, CA, USA), 0.15 µl of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... growth medium was replaced with differentiation medium consisting of 2% horse serum (Life Technologies, Carlsbad, CA) and 0.1% penicillin/streptomycin in high glucose DMEM to induce myotube formation ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were visualized on 2% agarose gel stained with SYBR® Safe (Invitrogen, CA USA).
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bioRxiv - Immunology 2021Quote: ... The reaction mixture contained 2×SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA), primer pairs and cDNAs ...
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bioRxiv - Immunology 2021Quote: ... incubated with SARS-CoV-2 Nucleoprotein polyclonal antibody at 1:500 dilution (Invitrogen, Carlsbad, CA, USA) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM L-glutamine (PAA Laboratories GmbH, Germany) and N2-supplement (Life Technologies Invitrogen, Carlsbad, CA) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM L-glutamine (PAA Laboratories GmbH, Germany) and N2-supplement (Life Technologies Invitrogen, Carlsbad, CA) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and gels were dry blotted onto nitrocellulose membranes (iBlot 2 NC Ministacks, Invitrogen, Carlsbad, CA USA) by the iBlot 2 transfer system (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... isolated FDB fibers were loaded with 4 µM fura-2 AM (Thermo Fisher, Carlsbad, CA, USA) in Ringer’s solution at room temperature (RT ...
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bioRxiv - Microbiology 2024Quote: Total RNA from tissues and HK-2 cells was isolated using TRIzol (Invitrogen, Carlsbad, CA, USA). Complementary DNA (cDNA ...
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bioRxiv - Plant Biology 2020Quote: DNA fragment lengths were determined on an ABI 3500xl Genetic Analyzer from 1 μL of 1:99 diluted PCR products mixed with 8.9 μL of HiDi Formamide (Life Technologies), and 0.1 μL of Gene Scan 500 LIZ Size Standard (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The N- and C-terminus of thyA (lpg2868) were amplified by BKMP94-95 and BKMP98-99 primers and introduced into pDonorP1-P4 and pDonorP3-P2 (Invitrogen) to generate pMME1084 and pMME1094 ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were heated to 99 °C for 15 min and aliquots (5 µL) resolved by SDS-PAGE on 8 % – 16 % Tris-glycine minigels (Invitrogen) (150 V ...
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bioRxiv - Immunology 2021Quote: ... The RNA was then dissolved in a 10μL mixture of 99 μL of DNase/RNase/Nucleotide-free water and 1 μL of SUPERase•In™ (Invitrogen, Life Technologies Incorporated ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... 24h after plating were incubated for 2h in LysoTracker Red DND-99 (75 nM) and for 45 min in Hoechst 33342 (Invitrogen) (0.25μg/ml).
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bioRxiv - Biophysics 2022Quote: ... 0.020 M potassium phosphate monobasic), sodium chloride (BioXtra grade, purity ≥99.5%) and D-(-)-Fructose (HPLC grade, purity ≥99%) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated under heating from 25 °C to 99 °C in StepOne Plus Real-time PCR system (Thermo Fisher Scientific). The kinetics of fluorescent signals were analyzed to calculate Tm values in Protein Thermal Shift Software 1.3 (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2022Quote: ... The sections were then cleared and stained in Clearsee solution with Basic Fuchsin (Fisher scientific Cat no 632-99-5) for 30 minutes followed by two washing steps in Clearsee only ...
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bioRxiv - Biochemistry 2024Quote: ... at 100 µg/mL in complete DMEM medium for 4 h and treated with LysoTracker Red DND-99 (Invitrogen #L7528) for 15 min and subsequently imaged on a Nikon CSU-W1 SoRa microscope ...
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bioRxiv - Molecular Biology 2023Quote: ... immediately followed by a triple MS2 stem-linker sequence (PNAS September 17, 2002 99 (19) 12203-12207) was purchased from ThermoFisher. This was used as a template to make pcDNA3.0_NORAD_NRU78_MS2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The measurement of lysosomal pH and Cathepsin B activity was evaluated with LysoTrackerTM Red DND-99 probe (InvitrogenTM, ThermoFisher Scientific) and the Magic Red® kit (Immunochemistry Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Lens apoptosis was determined in the same SAG and control DMSO embryos as heart eye measurements were made by staining with 5 μg/ml Lysotracker Red DND 99 (Invitrogen) for 30 min in the dark as described previously (Ma et al. ...
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bioRxiv - Microbiology 2024Quote: ... Phosphopeptide enrichment was carried out on the supernatant as described in 99 using the KingFisher Apex robot (Thermo Fisher Scientific) with 25 µl of Fe-NTA MagBeads (PureCube ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondly a PCR was performed using the same primer 99.R (Table 3) and Taq polymerase (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 90% confluent PNPs were washed with PBS and treated with TrypLE express (Thermo Fisher Scientific) at 37oC for 3-5 mins ...
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bioRxiv - Biochemistry 2020Quote: ... Substrates were PAGE purified to >90% purity using 10% non-denaturing polyacrylamide gel electrophoresis (Invitrogen). The biotin-labeled substrate was incubated with a 2-fold molar excess of neutravidin before the primer extension assay ...
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bioRxiv - Microbiology 2020Quote: ... 90 minute) directly inline with a Fusion Lumos Orbitrap Tribrid mass spectrometer (Thermo Fisher Scientific) operated in data independent acquisition mode (DIA ...
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bioRxiv - Cell Biology 2021Quote: ... Freezing of iPSCs was performed using freezing media containing 90% foetal bovine serum (Gibco, 10270) and 10% dimethyl sulfoxide (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged 1:5 at ∼80-90% confluency with 0.25% Trypsin (15090-046; Gibco). Co-culture assays were performed by incubating cells in 2.5 μM CFSE (65-0850-84 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged 1:5 at ~80-90% confluency with 0.25% Trypsin (15090-046; Gibco). Cells were routinely screened for mycoplasma ...
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bioRxiv - Bioengineering 2020Quote: ... or in a 90°C oven (Isotemp Vacuum Oven Model 282A, Fisher Scientific, Massachusetts, USA) to facilitate accelerated degradation [52].
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bioRxiv - Plant Biology 2022Quote: ... 90 μL of each well was mixed with 10 μL of Presto Blue (ThermoFisher Sci) in a clean ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were allowed to reach 80 – 90% confluency and collected using RIPA buffer (ThermoFisher, #89900) or 1% NP-40 buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were either frozen 3 days after transduction in 90% SVF (Life Technologies, France) and 10% DMSO (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated PBMCs were then cryopreserved in solution containing 90% heat-inactivated FBS (HI-FBS) (ThermoFisher) and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2020Quote: ... B: 20 mM ammonium formate added in 90% ACN (cat No. A998, Thermo Fisher, USA), ammonium hydroxide (cat No ...
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bioRxiv - Genomics 2021Quote: Myoblasts were grown to 90-100% confluency in flasks mounted on slides (Thermo Scientific, 170920) then differentiated over 3 days as previously described ...
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bioRxiv - Genomics 2020Quote: Minigene constructs were transfected into >90% confluence HeLa cells using Lipofectamine 3000 (ThermoFisher Scientific, L300015). Total RNA was isolated after 24 h transfection using TRIzol (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... at 2500rpm for 90 minutes followed by selection in 10μg/mL Blasticidin-S-HCl (Gibco). Guide RNA (gRNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were harvested in culture medium [90% Leibovitz’s L-15 medium with L-glutamine (ThermoFisher), pH 9.0 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were cultured until 90 % confluency and detached from T75 flasks using TrypLE (Life Technologies), and subsequently centrifuged at 250 g for 5 min and counted using a hemocytometer.
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bioRxiv - Developmental Biology 2022Quote: ... RNPs were assembled by adding 90 pmol TrueCut Cas9 v2 protein (Thermo Fisher, Cat #A36499) to 180 pmol of sgRNA and incubating at room temperature for 15 min ...