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Citations for Thermo Fisher :
9901 - 9950 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... VEEV-TC-83-nLuc RNA was transcribed in vitro from cDNA plasmid templates linearized with MluI via MEGAscript SP6 kit (Invitrogen #AM1330) and electroporated into BHK-21 cells ...
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bioRxiv - Biophysics 2021Quote: MRC5 cells (human lung fibroblasts, ATCC® Cat# CCL-171™, RRID:CVCL 0440) were maintained in MEM media (Cat# 11095080, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... h-iECs were dissociated into single cells and sorted into CD31+ and CD31-cells using magnetic beads coated with anti-human CD31 antibodies (DynaBeads, ThermoFisher, Cat No. 11155D). The purified CD31+ h-iECs were then expanded in culture on 10-cm dishes coated with 1% gelatin ...
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bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml for TNF-α assessment in myeloid cells or Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) for IFN-γ assessment in T-cells in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Genomics 2020Quote: Optimal establishment of primary human hepatocytes in tissue culture required a substratum of type 1 collagen (24-well plates; ThermoFisher Scientific #A11428-02) and initial plating at 200,000 cells/well in complete Williams’ E medium containing supraphysiological concentrations of insulin ...
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bioRxiv - Genomics 2020Quote: Small-interfering (si)RNA knockdown of LOC157273 was performed on primary human hepatocytes from donor Hu8200 (genotype G/G at rs4841132) plated into collagen-coated 6-well plates (ThermoFisher Scientific #A11428-01). Cells were incubated in complete Williams’ E medium and transfected with siRNA (50 nM ...
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bioRxiv - Bioengineering 2019Quote: ... Jurkat and Human B-lymphoid cell lines were initially tested for mycoplasma contamination and then cultured in RPMI-1640 medium (Life Technologies, Zug, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... B lymphocytes were obtained by negative cell selection using the MagniSort Human B-cell enrichment kit II (Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: The cytotoxicity of fluoro-TEMPO and CTEMPO against human T24 urinary bladder epithelial cells was examined utilizing the Pierce™ LDH cytotoxicity assay kit (Life Technologies, Australia) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with Aß-S8C dimer was subjected to hybridization in duplicates on the GeneChip PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Biochemistry 2019Quote: For mammalian expression a DNA polynucleotide encoding the transmembrane domain (residues 1-295) of GtACR1 optimized for human codon usage was cloned into the pcDNA3.1 vector (Life Technologies, Grand Island, NY) in frame with an EYFP tag ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2019Quote: ... was performed on the Rituximab expressing cell pool by staining with an anti-human IgG antibody conjugated to FITC (Invitrogen, Carlsbad, CA, USA) and subsequent single-cell sorting using fluorescence activated cell sorting (FACS ...
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bioRxiv - Cell Biology 2019Quote: Human RBCs (1% in PBS) were double labeled with the lipidic dye R18 and the content marker calcein-AM (Molecular Probes, Life technologies). 20 µl of R18 (1 mg/ml in ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... gondii parasites were maintained by serial passage in confluent human foreskin fibroblast (HFFs, ATCC# SCRC-1041) monolayers in Dulbecco’s Modified Eagle’s Medium (DMEM, Life Technologies-Gibco, Cat# 10569-010), supplemented with 1% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... labeled cDNA targets were generated with the Encore® Biotin Module (Nugen) and hybridized to a GeneChip® Human Transcriptome Array 2.0 (Affymetrix, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: The human hepatocellar carcinoma (HCC) cell line SNU-398 was cultured in Roswell Park Memorial Institute 1640 medium (RPMI) (Life Technologies, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: MOLM-13 cell clones cultured on-chip for 72 h were stained for the hematopoietic marker CD45 using a PE-conjugated primary antibody (mouse anti-human CD45-PE, Invitrogen #12-0451-82). Prior to imaging ...
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bioRxiv - Bioengineering 2020Quote: CD4+ T cells were electroporated prior to stimulation with anti-CD3/CD28 beads (Dynabeads Human T-Activator CD3/CD28, Thermo Fisher Scientific (ThermoFisher), Waltham ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 ng of labelled probe was combined with 5 μg of salmon sperm and 10 μg of human Cot1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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bioRxiv - Genomics 2021Quote: ... The over-expression construct of human LY6 was created by PCR amplification of cDNA by Phusion® High-Fidelity DNA polymerase (Thermo Fisher Scientific), using the following primers LY-6S-Pac1-S-5’-GGAACGTTAATTAACGCTGAAGTTTGTCTGTGCACTA-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... H1944 (human lung adenocarcinoma, ATCC, CRL-5907), and H441 (human lung adenocarcinoma, ATCC, HTB-174) cells were maintained in RPMI 1640 medium (Thermo Fisher Scientific, A1049101) with 10% FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells (HEK293T, ATCC, Cat #CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The iPSCs were cultured on irrMEF with medium for human iPSCs : KnockoutTM-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% KnockoutTM-Serum Replacement (KO-SR ...
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bioRxiv - Systems Biology 2021Quote: ... MDA-MB-231 human breast cancer cells were cultured in DMEM/F12 (1:1) media52 (Thermo Fisher Scientific, Life Technologies, Item #11330-032) supplemented with 10% Fetal Bovine Serum (Atlanta Biological ...
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bioRxiv - Bioengineering 2021Quote: ... MDA-MB-231 (ATCC) and MCF7 (ATCC) human mammary cancer cells were both cultured in 1:1 DMEM/F-12 (Thermo Fisher Scientific, USA) media supplemented with 10% fetal bovine serum (Atlanta Biological ...
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bioRxiv - Immunology 2021Quote: ... The pcDNA 3.1+ vectors were then transfected individually into human embryonic kidney (HEK293T) suspension cells using ExpiFectamine 293 transfection reagent (ThermoFisher Scientific, Waltham, MA, USA) following manufacturer’s specifications ...
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2021Quote: The whole genome siRNA screen was conducted as described (35) using HeLa S3 cells and the Dharmacon ON-TARGETplus® SMARTpool® human siRNA library (Thermo Scientific) and strain T3SA+.
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bioRxiv - Cancer Biology 2020Quote: ... human NSCLC cell lines and HARA human lung squamous carcinoma cell line were maintained in RPMI-1640 supplemented with 10% FBS (Gibco BRL, Gaithersburg, MD). Cell transfection was carried out by Lipofectamine 2000TM (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and after 24h of incubation fresh medium was added and cells were transfected with 100ng of total RNAs from cell cultures or human tissues using Lipofectamine 2000® (11668-019, Thermo Fisher Scientific). As positive controls ...
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bioRxiv - Cell Biology 2021Quote: Human epithelial colon adenocarcinoma, Caco2, cells (ACC 169, DSMZ, Leipzig, Germany) were grown in DMEM/F-12 (Gibco, Thermo Scientific, Waltham, Massachusetts, USA) supplemented with 10 % heat inactivated fetal bovine serum (Biowest ...
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bioRxiv - Cancer Biology 2021Quote: ... and metastasis human colon cancer cell line (SW-620) was cultured in Roswell Park Memorial Institute medium 1640 (RPMI 1640: Gibco, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (FBS ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... using a commercial human fetal brain cDNA library containing cDNAs fused to the gal4 activation domain of pEXP-AD502 (ProQuest™, ThermoFisher Scientific, USA), as prey ...
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bioRxiv - Cancer Biology 2022Quote: ... A CRISPR based kinome-wide screening was performed using a lentiviral sgRNA library (LentiArray™ Human Kinase CRISPR Library, Thermo Fisher Scientific, M3775) that knocks out 840 kinase and kinase related genes individually with total number of 3214 sgRNA constructs ...
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bioRxiv - Immunology 2021Quote: ... Fresh human peripheral blood mononuclear cells were used as effector cells after red blood cell lysis with ACK lysing buffer (ThermoFisher Scientific, Waltham, MA). After 1 h incubation at 37°C ...
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bioRxiv - Neuroscience 2021Quote: Post-selection iPSCs with stably-integrated human NGN2 under a tetracycline-inducible promoter were dissociated into single cells using Accutase (Thermo Scientific Cat. #A1110501) and plated on a Matrigel (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained for 30 min on ice using AlexaFluor700-conjugated anti-Human CD54/ICAM-1 (clone 1H4, Life Technologies, #MA528553, 1/75), PE-Cy7-conjugated anti-Human CD106/VCAM-1 (clone STA ...
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bioRxiv - Neuroscience 2021Quote: Lund human mesencephalic (LUHMES) neuronal precursors were grown in T75 flasks in proliferation medium (DMEM/F12 GlutaMAX™ supplement medium (Thermo Fisher Scientific), N2 supplement (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Panc1 and MiaPaCa2 with RBFOX2 knockdown and non-targeting control were generated using human Affymetrix Clariom D Arrays (Life Technologies, Cat. no. 902922) with the standard input kit run on the Applied Biosystems GeneChip 3000 instrument ...
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bioRxiv - Cell Biology 2022Quote: ... MCF10A human breast epithelial cells (CRL-10317, ATCC, Manassas, VI, USA) were maintained in DMEM/F12 medium (11039-021, Invitrogen, Carlsbad, CA, USA) supplemented with 20 ng.ml-1 epidermal growth factor (AF-100-122 ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: ... All primer pairs were tested alone and in multiplexed PCR reactions using 10 ng of TaqMan® Control Human Genomic DNA (Thermo Fisher Scientific) in 10 μl reaction volumes ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Immunology 2021Quote: ... 32,33 were maintained by serial 2-day passaging in human foreskin fibroblast (HFF-1, ATCC® SCRC-1041™, American Tissue Culture Collection) monolayers cultured in DMEM (Thermofisher scientific) with 10% fetal bovine serum (FBS ...