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Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The PACRG /Myc and DNALI1/Flag plasmids were co-transfected into COS-1 cells by sing Lipofectamine™ 2000 transfection reagent (Invitrogen). After 48 h transfection ...
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bioRxiv - Immunology 2022Quote: ... The three Fabs were expressed by co-transfection of plasmids of heavy and light chain at the ratio of 2:1 in ExpiCHO expression system (Life Technologies) for two weeks according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2022Quote: ... were transfected in suspension with 4.0 µg/well of SARS-CoV-2/mCherry-Nluc BAC plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Transfection media was changed to post-infection media (DMEM containing 2% FBS and 1% PSG ...
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bioRxiv - Developmental Biology 2022Quote: ... Wild-type or dominant-negative GJA1 mRNAs were transcribed from the cloned plasmid using an mMESSAGE mMACHINE™ SP6 Transcription Kit (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids were utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen #10359016). Exponentially growing Spodoptera frugiperda 9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium #BELN12-730Q ...
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bioRxiv - Developmental Biology 2019Quote: ... and pgam2 (CCTGGAGGAGGCGAAACG) were subcloned into plasmid pDR274 and transcribed into mRNAs in vitro by the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies). The linearized plasmid pZα-Cas9 ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed with PBS and placed in contact with duplexes of 1 µg of plasmid and Lipofectamine 3000 (Life Technologies), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with appropriate plasmids using Lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, Thermo Fisher Scientific Inc., Waltham, MA, USA). 24 h post-transfection cells were subjected to live-cell confocal microscopy analyses.
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bioRxiv - Cell Biology 2019Quote: ... A donor plasmid comprising homology arms (700–800 bp on either side of the NIPBL coding sequence start site) and EGFP were cloned into plasmid pJet1.2 (Thermo Scientific, K1232). Cas9 guide RNA sequences were identified using the website crispr.mit.edu (guide A ...
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bioRxiv - Bioengineering 2019Quote: ... HEK293T cells were plated into 10cm dishes and transfected at ~ 60% confluency with SpCas9-gRNA-expressing plasmid (15 μg) using Lipofectamine 2000 (Life Technologies). For PAM validation at endogenous site ...
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bioRxiv - Immunology 2019Quote: ... On day 1 cells were transfected with 1 μg of each of the two guide RNA plasmids and 0.5 ug of pGFP2-C1 vector with a Neon Transfection System Kit and Neon Transfection System (Thermo Fisher Scientific) according to manufacturer’s guidelines as described for this cell line ...
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bioRxiv - Cell Biology 2019Quote: ... THD-EGFP wild-type or mutant sequences downstream of the Tet-On responsive element were cloned into the pcDNA5/FRT plasmid (Life Technologies) expressing the Tet-On transactivator from the mouse PGK promoter ...
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bioRxiv - Microbiology 2019Quote: ... A stable line expressing the recombinant protein was generated by co-transfection of pIEX-10: Pbc43-SP/TM and pIEX-10:Neo plasmid [11] using the Cellfectin® II Reagent (ThermoFisher) according to the manufacturers’ guidelines ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 0.06 μg of CMV-promoter driven plasmid encoding SARS-CoV-2 wild type or mutant spike variants using Lipofectamine™ 2000 transfection reagent (ThermoFisher). Pseudovirus was harvested by collecting supernatant 48 h after transfection and filter sterilized (0.44 μm ...
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bioRxiv - Bioengineering 2020Quote: ... The GFP fragment (942 bp) was produced by amplifying the pEGFP-C1 plasmid using polymerase chain reaction in the Proflex PCR system (ThermoFisher Scientific). The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
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bioRxiv - Microbiology 2019Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Microbiology 2021Quote: For GFP RIPs HEK-293Ts were transfected with 2μg GFP or GFP-ORF57 plasmids with DNA in a 1:2 ratio with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were lysed before incubated with GFP-Trap Agarose beads overnight at 4 °C (Chromotek ...
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bioRxiv - Biophysics 2020Quote: ... After 12-16h cells were transfected with actinin-mEOS2 or Lifeact-mEOS2 (Supplementary information: Plasmid sequences) with a Lipofectamine 3000 (Thermo Fisher) according to the protocol provided by manufacturer ...
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bioRxiv - Synthetic Biology 2020Quote: All in vitro RNA editing experiments were carried out in HEK 293FT cells seeded in 24 well plates using 1500ng total plasmid and 2ul of commercial transfection reagent Lipofectamine 2000 (Thermo Fisher). Specifically ...
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bioRxiv - Synthetic Biology 2020Quote: RNA editing experiments for targeting 5’-GA-3’ were carried out in HEK 293FT cells seeded in 24 well plates using 1000ng total plasmid and 2ul of commercial transfection reagent Lipofectamine 2000 (Thermo Fisher). Specifically ...
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bioRxiv - Molecular Biology 2021Quote: ... The two sgRNA expression plasmids were amplified using Subcloning Efficiency™ DH5α™ Competent Cells (Thermo Fisher Scientific, Carlsbad, CA, USA). Midi-preparations of plasmids were made with the NucleoBond Xtra Midi kit (Macherey- Nagel ...
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bioRxiv - Molecular Biology 2021Quote: ... along with 50 ng and 100 ng of expression plasmids encoding 3xHA-tagged wildtype or mutant mouse PORCN-D as indicated using Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of 100 ng DHFR plasmid was amplified with 0.4 units of Phusion™ High-Fidelity DNA polymerase (Thermo Fisher), 4 μl of 5x Phusion™ HF buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were first transfected with siRNA for 24 h and then transfected with different plasmids by Lipofectamine 2000 (Invitrogen, 11668019) again ...
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bioRxiv - Developmental Biology 2019Quote: ... the newly generated pB-TRE3G_Kdm4b-Egfp vector was isolated using a PureLink™ HiPure Plasmid Filter Kit (Thermo Fisher, New Zealand) and validated by restriction enzyme digestion and sequencing analysis.
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bioRxiv - Immunology 2020Quote: Aag2 cells were seeded at 1 × 105 cells/well in 96-well plates and transfected at the time of seeding with 10 ng pKM50 (Renilla luciferase transfection control) and 50 ng/well of the respective firefly reporter plasmid using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Aag2 cells were seeded at 1 × 106 cells/well in 12-well plates and transfected at the time of seeding with 3 μg protein expression plasmid using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The cells were transfected at a density of approximately 2×106 cells/ml with equal amounts of each plasmid using Expifectamine reagent (Thermo Fisher) according to the instructions provided by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... IgGs and 6xHis-tagged Fabs were expressed by transient transfection of paired heavy chain and light chain expression plasmids into HEK293-6E or Expi293 cells (Life Technologies). Fabs and IgGs were purified from transfected cell supernatants using Ni-NTA (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... the PSG3 plasmid was co-transfected into HEK293T cells along with JRFL or YU2 HIV envelope plasmids using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Cancer Biology 2019Quote: ... transient transfection of the cells with some indicated plasmids alone or in combination was also performed in the TurboFect Transfection Reagent (Thermo scientific) or another Lipofectamine®3000 Transfection Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... BP recombination was carried out according to the manufacturer’s protocol using equimolar amounts (100 fmol) of the attB-PCR product and the pDONR entry clone plasmid (Invitrogen, 12535-019). Components were incubated in TE buffer with BP Clonase enzyme mix and reaction buffer at 25°C for one hour ...
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bioRxiv - Cancer Biology 2019Quote: Luciferase reporter assays were performed by cotransfecting 293 cells with 8 µl of 5 µM miR-26a mimic or negative control mimic with 500 ng of psiCHECK2 reporter plasmids using Lipofectamine 3000 (Thermo Fisher) and performing the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Developmental Biology 2019Quote: ... 140 μg AAV9-Rep/Cap, and 320 μg pHelper (pAd-deltaF6, Penn Vector Core) plasmids were produced by maxiprep (Invitrogen, K210017) or Gigaprep (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... by stable transfection of a aVb3 expression plasmid and thus required antibiotic selection with 0.5 mg/ml Geneticin (Thermo Fisher, USA). If not otherwise stated ...
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bioRxiv - Synthetic Biology 2019Quote: ... and cloned using isothermal assembly.43 Relevant plasmid sequences are included in the Supplementary Table 2 All plasmids were purified for cell-free expression using Qiagen Plasmid Midiprep Kit (Cat. 12145) and quantified by Qubit dsDNA BR Assay Kit (Invitrogen #Q32853).
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bioRxiv - Genetics 2020Quote: ... three pX330-based plasmids encoding sgRNAs targeting each gene were transfected into McA-RH7777 cells using lipofectamine 3000 (Thermo Fisher Scientific). Transfected cells were seeded sparsely in 10 cm dishes to obtain colonies ...
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bioRxiv - Genetics 2020Quote: ... HEK293T cells were plated into 10 cm dishes and transfected at 50∼60% confluency with Cas9-gRNA-expressing plasmid (15 μg) using 30 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM ...
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bioRxiv - Genetics 2020Quote: Cas9 mRNA for zygotic injections was generated from the XhoI-digested pCMV/T7-hCas9 plasmid (Toolgen) using the mMESSAGE mMACHINE® T7 ULTRA Transcription Kit (Ambion) and purified using RNeasy Mini Kit (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA (2 μg of each plasmid) and FuGENE (3 μL FuGENE/μg DNA) were mixed thoroughly in 100 μL Opti-MEM (Thermo Fisher) and incubated at room temperature for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Transformants with pPICZc plasmid were selected on yeast extract peptone dextrose (YPD) solid media containing 100 mg/l Zeocin (Gibco, #R25001). For transformation of cells with pIB2 or pIB4 plasmids ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: Transfection of sgRNA-Cas9 plasmids into Calu-6 cells for individual gene validation was done using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Expression characteristics of each plasmid were evaluated in HEK 293T cells by transient transfection using Lipofectamine™ 3000 (Thermo Fisher Scientific).
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bioRxiv - Genomics 2021Quote: ... We co-transfected gRNA_cloning vectors (1 µg each) and pSpCas9(BB)-2A-Puro plasmid (1 µg) using lipofectamine 3000 reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... U2-OS and HEK293T cells were transfected with dCas9-3xGFP and C9-sgRNA plasmid at a 1:10 ratio using Lipofectamine 3000 (Thermo Fisher).
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bioRxiv - Physiology 2020Quote: ... The purified PCR products were cloned into plasmid vectors (pCR 2.1 TOPO TA, Invitrogen, and pJet 1.2/blunt, Thermo Fisher Scientific, USA) that were then used to transform electro-competent DH5α or DH10B Escherichia coli ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... CHO-K1 and furin-deficient CHO-FD11 cells were cultured at 37 °C and 5% CO2 in Ham’s F-12 medium supplemented with 10% (v/v) FBS 44 and transfected with 3 μg of plasmid following the standard protocol for Lipofectamine 2000 (Thermo Fisher). For the PCs inhibition ...
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bioRxiv - Immunology 2020Quote: ... A pair of plasmids separately expressing heavy and light chain of antibodies were transiently co-transfected into Expi293™ cells (Catalog No. A14528, ThermoFisher) with ExpiFectamine™ 293 Reagent ...
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bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the cytomegalovirus (CMV) promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen, Carlsbad, CA). The Fbxl22 RNAi plasmid encodes EmGFP and an artificial pre-miRNA targeting the full length gene of mouse Fbxl22 under bicistronic control of the CMV promoter ...