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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cells were treated with compound at a final DMSO concentration of 2% prior to transfection with 100 ng of the wild-type or triple mutant (L50F E166A L167F) plasmid with Lipofectamine LTX (ThermoFisher#15338100) for 24h ...
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bioRxiv - Cancer Biology 2022Quote: HeLa cells were transfected with 20 g of empty vector pCMV6 (PS10001) or 18 g of FLOT2 (RC220884) plasmid with Lipofectamine LTX Plus Reagent (15338100, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentiviral particles were produced in HEK293T cells using 2nd generation packing plasmids and Calcium Phosphate Transfection Kit (Invitrogen, Carlsbad, CA, US). K562 cells were transduced as described previously27 ...
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bioRxiv - Biophysics 2022Quote: A 250 mL culture of Expi293 cells at 3e6 cells/mL was transfected with 100 μg each of the heavy chain and light chain plasmids (pMAS478, pMAS481) in 20 mL of Opti-MEM (Life Technologies) supplemented with 200 μL of FectoPRO (Polyplus) ...
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bioRxiv - Biophysics 2022Quote: ... Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco, 31985070) according to the Lipofectamine 2000 instructions ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmids were purified using Qiagen Maxi Kit and quantified using a Nanodrop spectrophotometer (Thermo Fisher Scientific, Catalog No: ND-2000). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... coding sequences of CMV MPs and CmVPS41s were PCR amplified using specific primers (Supplemental table 1) and cloned into plasmid pENTR/D-TOPO (Thermo Scientific by Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Linearized template DNA (amplified from plasmid with T7/SP6 primers) was used to synthesize RNA probes with either T7 or SP6 RNA polymerase (Life Technologies), and DIG-labeling mix (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... 50–60% confluent cells were transfected with 2.4 μg plasmid DNA using 8.5 μl Lipofectamine™ 2000 transfection reagent (Thermo Fisher Scientific) in 300 μl Opti-MEM reduced serum medium (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: We used 1.2 × 106 HEK293T cells to transfect the base editor plasmids (2000 ng) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by cotransfecting paired heavy and light chain expression plasmids into Expi293F cells using ExpiFectamine 293 transfection reagents (ThermoFisher Scientific), purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare) ...
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bioRxiv - Genetics 2022Quote: ... a ben-1 expression plasmid was combined with the myo-2p::GFP co-injection marker (5 ng/uL) and 1 kb DNA ladder (Invitrogen #10787018) at the specified concentrations (Table S2 ...
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bioRxiv - Microbiology 2022Quote: ... Transfection conditions used a ratio of 1 µg total DNA (including all three plasmids) for 3 µl Lipofectamine 2000 that were mixed in the presence of Opti-MEM (Life Technologies) as described previously.36 ...
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bioRxiv - Microbiology 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
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bioRxiv - Immunology 2022Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 μg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting solution was used to perform multiplex PCR using primer pairs specific for each endogenous plasmid of strain B31 [61] and the DreamTaq Green DNA Polymerase (Thermo Scientific). For genomic DNA extraction ...
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bioRxiv - Microbiology 2022Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Systems Biology 2022Quote: ... Individual wells were transfected with 25ng of plasmid expressing one tagged Parkin variant using a Lipofectamine 3000 kit (Invitrogen, L3000-015) via manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were heat shocked into Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific, Cheshire, UK) and purified as previously described82 ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting plasmid was used to transfect HEKR4 PTC reporter cells in 6-well plates with Lipofectamine 2000 (Invitrogen, Cat# 11668019) and PLUS™ Reagent (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was cloned into the universal domestication plasmid pUPD2 by restriction ligation reaction with BsmBI and T4 ligase (both Thermo Scientific), and selected clones were verified by Sanger sequencing (Eurofins ...
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bioRxiv - Plant Biology 2022Quote: the vector pENTER223 containing the coding sequence of AtMYB68 (G22683) was purchased and recombined in the pFK247 plasmid using the Gateway® (Invitrogen) system.
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bioRxiv - Plant Biology 2022Quote: ... the insert was transferred to binary plasmid pGWB3 for the expression under CaMV35S promoter through LR clonase reaction (Thermo Fisher Scientific). Chickpea transformation was done with these constructs ...
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bioRxiv - Neuroscience 2022Quote: ... 25 ng of plasmids containing sgRNAs for respective genes were transfected into rat primary neurons on DIV 4 using Lipofectamine 2000 (ThermoFisher, #11668027) along with 50 ng of TDP43(WT)-EGFP or mApple then survival was measured over the course of 10 days ...
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bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40 and MERS-CoV UpE targets were transcribed from 600 ng of linearized and purified plasmids using the TranscriptAid T7 High Yield Transcription kit (Thermo Scientific). The IVT mastermix was set up according to the manufacturer instructions and the reaction was performed at 37°C for 6-7 hr ...
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bioRxiv - Microbiology 2022Quote: BHK-21 cells were transfected with 1 μg of pCAGGS plasmid encoding the NL09 wild-type or escape mutant HA proteins using Lipofectamine (Invitrogen, USA) and Plus reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... with 250ng each of the 8 plasmids corresponding with each segment of viral genome (or 7 plasmids for mock) and 4 µl Lipofectamine 2000 transfection reagent (Thermo Fisher) per well ...
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bioRxiv - Neuroscience 2022Quote: ... 2 µg of plasmid and 6 µg of PEI were diluted in 0.5 mL of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... each DNA fragment was blunt-end ligated into the pCR2.1-TOPO-TA or pCR-Blunt-II-TOPO plasmid vector (Invitrogen, Waltham, USA). Ligation was performed using the TOPO TA or Zero Blunt TOPO PCR Cloning Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... MSI2 pRD-RIPE plasmids were electroporated into the cell line using the Neon electroporation system (Invitrogen, 1100V 30ms and 2 pulses) and allowed to grow at 37°C for one week ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and transfected with 1 μg of Edit-R SMART Cas9_mCMV_(PuroR) expression plasmid (U-005200-120, Dharmacon) using 5 μL of Lipofectamine 2000 (11668027, Thermo Fisher Scientific) in 400 μL of Opti-MEM (31985062 ...
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bioRxiv - Neuroscience 2022Quote: ... capped and polyadenylated mRNA was synthesised in vitro from plasmid stocks using the mMESSAGE mMACHINE™ SP6 transcription and poly(A) tailing kits (Invitrogen), which was subsequently diluted to a standard concentration of 200 ng/μL (100 ng/μL for GFP mRNA) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were placed in OPTI-MEM media in the presence of the plasmid and Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA). Transfection was achieved following the manufacturer’s protocol ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... the neurons were given 12 hours in growth media at 37°C/5% CO2 to allow sufficient recovery and expression of the plasmid prior to fixation in 4% formaldehyde (Thermo Fisher) / 4% sucrose (VWR ...
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bioRxiv - Synthetic Biology 2024Quote: ... encoded on pcDNA-based plasmids were co-transfected with their respective transposon vector by lipofection with Lipofectamine LTX with PLUS Reagent (ThermoFisher 15338100). 100 ng of transposase expression vector was mixed with 500 ng of reporter-containing integration vector ...
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bioRxiv - Cell Biology 2023Quote: ... Tetracycline inducible HeLa EPHB2-FLAG cells were generated by transfecting Flp-In T-REx HeLa cells with EPHB2-BirA*-FLAG expression plasmid using Lipofectamine 3000 (Invitrogen, #L3000015) followed by hygromycin selection (200µg/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... Transient transfections were accomplished with Lipofectamine LTX using 5 μg plasmid DNA per million cells in Opti-MEM reduced serum media according to manufacturer’s instruction (ThermoFisher, Waltham, MA). After transfection ...
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bioRxiv - Microbiology 2024Quote: Mutations in Htc10-LBD were generated through site-directed mutagenesis by PCR (Quick Change) using plasmid pFR2 as template and DNA polymerase Pfx (Thermo Fisher). Oligonucleotides carrying the desired mutations are listed in Table S1 ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
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bioRxiv - Molecular Biology 2024Quote: ... 293T human cells were seeded onto coverslips in a 6-well plate followed by transfection with the GFP plasmids noted above using Lipofectamine 3000 reagent (Thermo Fisher). For mitochondrial localization ...
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bioRxiv - Cell Biology 2024Quote: ... The pLV vectors were co-transfected with packaging plasmids (Celecta #CPCP-K2A) into HEK 293T cells using Lipofectamine 3000 reagent (Invitrogen #L3000001) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were used as host cells to transfect the SARS-CoV-2 FSE dual luciferase reporter plasmid using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific). The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al ...
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bioRxiv - Immunology 2024Quote: ... Transfections of PhoenixA cells were carried out in 100-mm cell culture dishes with pBMN-I-EGFP construct plasmid DNA (20 μg) and 40 μl of Lipofectamine 2000 reagent (Life Technologies). Transfected cells were cultured in DMEM medium for two days before culture supernatants containing retroviral particles were harvested ...
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bioRxiv - Neuroscience 2024Quote: ... Expression plasmid of HyPer7 was designed based on reported mammalian expression plasmid for HyPer7 (Pak et al. 2020) and was synthesized by Thermo Fisher Scientific with codon optimization for gene expression in C ...
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bioRxiv - Microbiology 2024Quote: ... the complete segments of IBDV Soroa were amplified from viral dsRNA using a sequence independent amplification method (Potgieter et al., 2009) and cloned in plasmid pJET1.2 (Thermo Fisher Scientific). The hammerhead ribozymes ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... Neuro-2a cells were cultured on 6-well plates and then transfected with a total of 2 µg of an expression plasmid using Lipofectamine 2000 (Invitrogen, USA) as per the instructions provided by the manufacturer.