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Citations for Thermo Fisher :
9851 - 9900 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 3×105 cells were seeded in a 6-well plate and transfected 24h later with 5 µg pX459 plasmid DNA using Lipofectamine 3000 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: RNA template sequences were ordered as dsDNA gBlocks that contain the T7 promoter from Integrated DNA Technologies (IDT) and inserted into a linearized pRZ plasmid using the infusion cloning kit (Thermo Fisher) and transformed into Sure2 cells (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... Flp-In HEK293 cells were co-transfected with the pCDNA3-TurboID-cyclin F plasmid and the pOG44 Flp-Recombinase Expression Vector (Invitrogen, V600520) for co-expression of the Flp-recombinase using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... LifeAct and ARPC4 CDS cDNAs were subcloned into Gateway pENTR plasmids and introduced into transient expression vectors or lentiviral constructs by LR recombination (Thermo Fisher) to generate EGFP ...
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bioRxiv - Molecular Biology 2023Quote: ... RBM17- and SAP30BP-depleted HeLa cells were transfected at 48 h post-transfection with 0.5 µg of mini-gene plasmid (Supplementary Table S2) using lipofectamine 2000 reagent (Invitrogen–Thermo Fisher Scientific). These cells were incubated for 24 h prior to the extraction of RNAs described previously6 ...
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bioRxiv - Microbiology 2023Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Genomics 2023Quote: ... 1.2×105 cells per well were plated on coverslips coated with 0.1% gelatine in 12-well plates and transfected with 500ng of plasmid DNA per well using Lipofectamine 2000 (Invitrogen, Cat# 11668019) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... CAG-T7-hspCas9-T2A-RFP-H1-MPV17L2-gRNA#1 and CAG-T7-hspCas9-T2A-RFP-H1-MPV17L2-gRNA#2 plasmids were transfected into HeLa S3 cells using Lipofectamine 3000 reagent (Thermo Fisher). RFP-expressing cells were concentrated by fluorescence-activated cell sorting and subjected to limiting dilution cloning ...
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bioRxiv - Bioengineering 2024Quote: ... and m6A-Tracer-NES plasmid (m6A-Tracer; addgene, #159607) were simultaneously transfected into HEK-293 cells using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000008). After 48-h co-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at 100,000 cells per well in 12-well plates 24 h prior to transfecting 1 µg of each plasmid using Lipofectamine 3000 (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Phoenix amphotropic cells were transfected with the pBMN plasmid containing the FAP gene (received from vectorbuilder) using Lipofectamine and Plus reagent (Life Technologies) as previously described59 ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmid p5E-vsx2 was generated by inserting a polymerase chain reaction (PCR)-amplified vsx2 promoter genomic fragment into p5E plasmid using BP clonase (ThermoFisher, 11789013). PCR reaction was performed using primers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA concentrations were converted to genome copies per milliliter by entering the genome length of the pGEM plasmid into the DNA copy number and dilution calculator by Thermo Fisher Scientific Inc for determining the number of copies of a template (https://www.thermofisher.com/il/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/dna-copy-number-calculator.html).
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bioRxiv - Neuroscience 2023Quote: ... and TfR-Halo were mixed with Lipofectamine 2000 (0.25μg per coverslip for each plasmid) in Opti-MEM (Gibco, Catalog number: 31985070), transfer the mixture into primary neurons for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... transduced cells with iTAP-FLAG-HA or iTAP-ALFA-HA plasmids were selected using 15 µg/ml blasticidin S and 2 µg/mL puromycin (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Tetracycline inducible HeLa EPHB2-FLAG cells were generated by transfecting Flp-In T-REx HeLa cells with EPHB2-BirA*-FLAG expression plasmid using Lipofectamine 3000 (Invitrogen, #L3000015) followed by hygromycin selection (200µg/ml) ...
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bioRxiv - Developmental Biology 2024Quote: All plasmids generated in this study were constructed using conventional restriction enzymes and CloneJet PCR Cloning Kit (Thermo Scientific™, K1231).
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bioRxiv - Biophysics 2024Quote: ... The concentration and purity of the purified plasmid DNA was assessed by measuring the absorbance ratio (A260/A280) using a Nanodrop spectrophotometer (Thermo Scientific) and aiming for a 1.9 ratio ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
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bioRxiv - Microbiology 2024Quote: Mutations in Htc10-LBD were generated through site-directed mutagenesis by PCR (Quick Change) using plasmid pFR2 as template and DNA polymerase Pfx (Thermo Fisher). Oligonucleotides carrying the desired mutations are listed in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... were purchased from Twist Biosciences to insert into various plasmids for tagging or overexpression using the Gateway cloning kit (ThermoFisher Scientific). These plasmids were pAG426GAL-ccdB ...
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bioRxiv - Immunology 2024Quote: ... Replication-competent VSV-G pseudotyped HIV-1 virus was prepared by co-transfecting 293FT cells with the respective HIV-1 molecular clone and VSV-G expression plasmid using Lipofectamine 3000 reagent (Life Technologies). Transfection media was replaced with fresh antibiotic free DMEM media 8 h post-transfection ...
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bioRxiv - Developmental Biology 2024Quote: Retroviral particles for the generation of DN-KASH and Delta-PP lines were generated in HEK293T cells expressing retroviral packaging plasmids (gift from N. Montserrat) and transfected using Lipofectamine™ 3000 Transfection Reagent (Invitrogen). The viral titer collected 2-days post transfection was used to infect Rex1-GFP mESCs overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... which were grown for maxiprep purification using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit (Thermo Fisher Scientifics, Waltham, MA).
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bioRxiv - Cell Biology 2024Quote: ... were transiently transfected with 300 ng of plasmid construct YFP-PARP14 MD1 Mut (Kind gift from Dr. Ivan Ahel, University of Oxford) using Lipofectamine 3000 (Invitrogen, L3000001) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Individual constructed lentiCRISPR-v2 vectors were co-transfected with psPAX2 and pMD2.G plasmids into 293T cells using Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... A549 and/or H1299 cells were cotransfected with 2 ug of NPRL2 plasmid DNA (pKGB2-NPRL2) and 1 ug of pcDNA3.1 vector (Invitrogen, Carlsbad, CA) containing a neomycin-resistant gene ...
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bioRxiv - Immunology 2024Quote: ... was synthesized using T7 promoter-flanked PCR products generated by amplifying a Luciferase-containing plasmid with T7-flanked PCR primers with the MEGAscript RNAi kit (Life Technologies) (Supplementary Table 5) ...
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bioRxiv - Cell Biology 2024Quote: ... Stable cell lines in this study were generated by stably transfecting plasmids containing gene of interests either by electroporation using Neon Transfection Kit (ThermoFisher; MPK1025) (RPE1 hTERT cells ...
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bioRxiv - Biochemistry 2024Quote: ... the donor plasmids pAH18 or pAH20 were co-transfected with the nuclease plasmid PX458-AAVS1 (a gift from S. Jakobs, Max Planck Institute for Biophysical Chemistry) using Lipofectamine 3000 (Thermo Fisher). Transfected cells were selected with 10 µg/mL blasticidin (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... 8% CO2 with 125 rpm rotation to a density of 3.0 x 106 live cells per mL of culture were transiently transfected with an appropriate plasmid at 1 μg of DNA per 1 mL of culture using the Expifectamine transfection kit (Thermo Fisher) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... mESCs were co-transfected with the two plasmids (px458-gRNA1 and px330-mCherry-gRNA2) by the use of Lipofectamine 200 following manufacturer’s instructions (Invitrogen; Cat #11668019). GFP+/mCherry+ single cells were FACS-purified ...
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bioRxiv - Molecular Biology 2024Quote: ... Transfection with 200 ng of (d)CasRx or other (d)Cas13 expression plasmids and 200 ng of the indicated gRNA was done with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells were plated at a density of 200,000 cells per well in a 24-well plate and transfected with 800 ng of dCasRx expression plasmid and 800 ng of gRNA using Lipofectamine 2000 (Life Technologies). Transfected cells were harvested 72 hours post-transfection.
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bioRxiv - Neuroscience 2024Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... Plasmid clones were picked and inoculated into liquid LB media for overnight culture followed by miniprep using the PureLink Plasmid MiniPrep Kit (Invitrogen; K210010), and verified using sanger-sequencing by Eurofins genomics (Louisville ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells were transfected with the PX459 plasmid containing sgARH3-2 (Table S2) using the Lipofectamine 3000 transfection reagent (Invitrogen #L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 reverse transcription products and standard plasmids were subjected to real-time PCR analysis (QuantStudio 7 Flex Real-Time PCR System; Applied Biosystems) using human β-actin as an internal control ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were co-transfected with 12 µg lentivirus expression plasmid and 4.5 µg each of the packaging and envelope plasmids psPAX2 and pMD2.G in a 10 cm dish with Lipofectamine 2000 (Invitrogen, 11668019) in Opti-MEM medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... 2X105 cells were seeded into a 6-well plate and transfected using a total of 2 µg of plasmid using Lipofectamine 3000 (Invitrogen, USA) as per the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: ... a Saccharomyces cerevisiae J694α (α mating type) library containing a plasmid (pDEST™22, GAL4 activation domain, AD; Thermo Fisher Scientific) collection of 1,200 Open Reading Frames of TFs in A ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol for a 25 mL culture ...
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bioRxiv - Genomics 2024Quote: 20 μg of retroviral plasmid DNA (sgGFP-Cr2 or sgZbtb20-Cr3-cs2) with 6 μg pCL-Eco plasmid DNA were transfected using 60 μl Lipofectamine 2000 (Invitrogen, #1668019) in 3 ml OptiMEM (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... DNA was extracted using a Zymo Research Plasmid Miniprep kit and concentrations were measured with a Nanodrop 2000c spectrophotometer (Thermo Scientific). Sequencing analyses were used to confirm successful plasmid generation.
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bioRxiv - Biochemistry 2024Quote: ... plasmid isolation and PCR product extraction from agarose gels were carried out using GeneJET kits (Thermo Fisher Scientific, USA, #K0503, #K0832). Site directed mutagenesis was carried out using Phusion™ Plus DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... sec-eGFP mRNA was transcribed from pCS2+-secEGFP plasmid (23) and prepared using Ambion mMESSAGE MACHINE kit (Thermo Fisher, Waltham, MA). 80 ng of sec-eGFP mRNA was injected into 1-2 cell stage wild type zebrafish embryos ...
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bioRxiv - Microbiology 2024Quote: ... a small fragment containing a single parS site was produced by PCR amplifying a region of the large plasmid containing 1x parS sites described above with Phusion High-Fidelity DNA Polymerase (Thermo Scientific) (Supp ...
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bioRxiv - Molecular Biology 2024Quote: ... or 2.5 µg (6-well plates) of plasmid DNA diluted in OptiMEM medium using the Lipofectamine 3000 reagent (Invitrogen, ThermoFisher Scientific, France) for 48 h ...
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bioRxiv - Immunology 2024Quote: THP1-Dual™ KO-STING Cells in 6-well plate were transfected with 1µg TMEM173 plasmid (in pcDNA 3.1 vector) with Lipofectamine LTX and Plus Reagent (Invitrogen, cat no: A12621) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... SLI for selection of parasites with the plasmid integrated into the genome was done as described (Birnbaum et al., 2017) by adding 400 μg/mL G418 (ThermoFisher; pSLI) or 0.9 μM DSM1 (Sigma Aldrich ...