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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... A total of 3×105 cells were electroporated with 900 ng sgRNA and 3000 ng linearized donor plasmid using the Neon Transfection System (ThermoFisher Scientific). Positive clones were enriched using a low-dose of G418 (200 μg/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... a heavy chain (HC) and light chain (LC) plasmid was generated for co-expression in HEK293 suspension culture cells (Expi293F cells) (Fisher Scientific). For species specificity swapping experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid was heat shocked into Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific, Cheshire, UK) and purified as previously described43 ...
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bioRxiv - Developmental Biology 2021Quote: The ocean pout polyA terminator (OPT) sequence was released from plasmid GBT-RP2 (Clark et al., 2011) via restriction digest with BfaI-FD (Thermofisher Scientific), ligated into pGGEV_5_linker (Addgene #49285) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected with 1 ug of pCAG-Rox-STOP-Rox-LoxP-DsRed-Stop-LoxP-eGFP-Stop and indicated plasmids using Lipofectamine 2000 (Invitrogen, Inc.). 24 hours after transfections ...
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bioRxiv - Biochemistry 2021Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mg of plasmid DNA (1 mg HsSLC7A11 + 1 mg HsSLC3A2) was diluted in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, UK) to a total volume of 30 ml ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells seeded in poly-D-lysine coated 100 mm dishes at ∼75 % confluency were washed five times with Opti-MEM and transfected using 24 µg of the S glycoprotein plasmid with Lipofectamine 2000 (Life Technologies). After 5 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were transiently transfected with plasmids expressing GSPT1-HA wild type and variants (6 µg) using lipofectamine 3000 according to manufacturer’s protocol (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Developmental Biology 2021Quote: ... then the plasmid was linearized with NotI and dCas9-Neon mRNA was synthesized in vitro with mMESSAGE mMACHINE™ SP6 Transcription Kit (Ambion). Single guide RNA (80nM/ul ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded at a density of 2.5 x 105 cells/well in a 6-well dish and transfected with CRISPR-Cas9 plasmids using the standard Lipofectamine 3000 (Thermo Fisher) protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and transfection was performed using the above described plasmids for 4 hours with Lipofectamine 3000 reagents (catalog #L3000008, Thermo Fisher Scientific) following manufacturer’s procedures ...
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bioRxiv - Synthetic Biology 2021Quote: ... All assembled plasmids (pFB66 to pFB72, pFB96 to pFB130) were checked correctly by agarose gel electrophoresis after FastDigest XbaI (Thermo Scientific) digestion ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were transfected with 5ug of pSpCas9(BB)-2A-GFP plasmid containing the respective sgRNA using Lipofectamine 3000 (Life Technologies, L3000008). After 24h incubation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK293T cells seeded on 48-well plates were incubated until 60% to 70% confluence and then were transient transfected with 0.01 μg pGL3-STK38 plasmid using Lipofectamine 2000 (Invitrogen, CA, USA). After transfection 36-48 h ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transfection of each plasmid (2.5 µg DNA/1.5 × 105 cells) was performed using Invitrogen Lipofectamine® 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The transfection of Huh7.5.1 cells and Huh7 cells in ribosome profiling and RNA-seq experiments were transfected with indicated plasmids using Lipofectamine 2000 (Life Technologies). si-RNA transfection was performed using MaxFection™ 8600 Transfection Reagent (Biomaterials USA ...
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bioRxiv - Immunology 2020Quote: ... The amplicons where then ligated into the pCR4-topo plasmid between the M13F and M13R sequences and transformed into DH5α competent cells following the manufacturer’s protocols (Invitrogen, 18265-017). Up to 8 colonies were selected for HLA-A ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... the plasmid complement of each isolated clone was determined using primer pairs previously described (77) and DreamTaq Green DNA Polymerase (ThermoFisher Scientific). Each clone was confirmed to contain all B31-specific plasmids ...
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bioRxiv - Genomics 2020Quote: ... plasmid encoding lentivirus was co-transfected with pMD2.G and pCMV-dR8.91 into the cells using Lipofectamine 3000 (Thermo Fisher, L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and directly ligated into either pYD1 or pYES2/NTC plasmids with compatible restriction ends using T4 DNA ligase (Thermo Fisher Scientific). The pYD1 expression vector is a low copy number plasmid designed for surface display of proteins by high-jacking the Aga1-Aga2 system of the yeast ...
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bioRxiv - Immunology 2021Quote: ... and firefly Luc reporter gene plasmid (pHR′ CMV Luc) (47) in a 1:17:17 ratio in Opti-MEM (Life Technologies). Transfection mixtures were added to pre-seeded HEK 293T/17 cells in T-75 flasks containing 12 ml of growth medium and incubated for 16-20 hours at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The GOIs were isolated from the purified plasmids by digestion with EcoRI (Esp3I for RUNX1T1) and Xbal according to the manufacturer’s protocol (FastDigest, Thermo Fisher Scientific). Positive clones were purified from a 1% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In T-REx 293 cells were co-transfected with pcDNA5/FRT/TO and pOG44 plasmids using Lipofectamine2000 (Thermo Fisher, 11668019). 24h after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... Selectively replicated pING vectors containing the lepB-secM(Ms) constructs were subsequently purified from the cultured cells using GeneJET Plasmid Miniprep Kit from Thermo Scientific, according to the supplier’s specifications ...
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The G2-phase enriched lncRNA SNHG26 is necessary for proper cell cycle progression and proliferationbioRxiv - Molecular Biology 2021Quote: Hek293T cells were plated 24 hours before transfection with Lenti-sgRNA puro containing the different gRNAs or pHR-SFFV-dCas9-BFP-KRAB or pXPR_120 together with the packaging plasmids psPAX.2 and pMD2.G using Lipofectamine 2000 (Invitrogen™, 11668019). Culture medium was replaced 7 hours after transfection to decrease toxicity ...
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bioRxiv - Microbiology 2021Quote: VEEV-TC-83-nLuc RNA was transcribed in vitro from cDNA plasmid templates linearized with MluI via MEGAscript SP6 kit (Invitrogen #AM1330) and electroporated into BHK-21 cells ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S gene containing plasmid p20017 and adenovirus backbone plasmid pAADV-C01 (Genemedi, China) were co-transfected into HEK293 based adapted viral production cell (ThermoFisher, USA). Viral production cells were seeded in a 6 well TC treated plate (Nest ...
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bioRxiv - Microbiology 2021Quote: PCR products and linearized plasmids for transformation were purified and concentrated using a GeneJET PCR purification kit (Thermo Fisher Scientific, Inc.). Electroporation was used to introduce the DNA into cells following a previously described method [73] ...
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bioRxiv - Genomics 2021Quote: ... The second fragment was the parent plasmid digested at two locations using the FastDigest enzymes Bgll and Bglll (Thermo Fisher Scientific). The final fragment was another region of the plasmid amplified via PCR ...
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bioRxiv - Cell Biology 2021Quote: ... A Lipofectamine2000 transfection solution was prepared according to the manufacturer’s instructions containing a total of 0.8 (for rescue experiments with either TANGO1S-mSc or TANGO1L-HA) or 1 μg plasmid DNA per construct and 2.5 μL lipofectamine2000 (Thermo Fisher Scientific) in 200 μL OptiMEM (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... 5 × 106 cells were plated in 10 cm dishes and co-transfected with 3 μg VSV-G plasmid and 9 μg of the respective construct Lipofectamine LTX (Thermo Fisher) the following day ...
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bioRxiv - Genomics 2021Quote: ... 600,000 cells were plated in 6 well plates and transfected with 2 μg plasmid DNA using 6 μl Lipofectamine LTX reagent (Thermo Fisher). Medium was exchanged after 16 h and harvested 24 h later ...
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bioRxiv - Molecular Biology 2021Quote: ... with the respective plasmids using the protocol provided with the FreeStyle 293 Expression System (Thermo Fisher Scientific, Cat. no. K9000-01). The isotypes contain human V regions from hybridomas that were established from a human HHKKLL Trianni mouse (Patent US 2013/0219535 A1) ...
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bioRxiv - Bioengineering 2021Quote: ... The standard curves were constructed from a series of 10-fold dilutions of plasmid DNA obtained by TOPO TA cloning (ThermoFisher, USA).
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bioRxiv - Cell Biology 2019Quote: ... Purified PCR products and fluorescent protein expression plasmids were digested with the corresponding restricted enzymes (ThermoFisher, see Table S1 for details) and then cleaned up with a PCR purification kit ...
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bioRxiv - Molecular Biology 2021Quote: ... pUC57-T7-vaultRNA1-1 plasmids were linearized and used for in vitro transcription of RNA using the MEGAshortscript kit (AM1354, Thermo Fisher) with 32P-αUTP (SRP-210 ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamine CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: Plasmids carrying the antibody heavy and light chain of nAbs were used for transient transfection of Expi293F™ cells (Thermo Fisher) as previously described22 ...
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bioRxiv - Microbiology 2022Quote: The linearized RG plasmids were used as templates to produce capped T7 RNA transcripts using T7 mMESSAGE mMACHINE™ T7 Transcription kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Expi293 cells were transfected with the expression plasmid hACE2-1-740aa-8xHis or hACE2-1-614aa-8xHis using ExpiFectamine 293 (ThermoFisher Scientific). Four days later ...
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bioRxiv - Neuroscience 2022Quote: ... and Gαq/s for LPAR2 and LPAR4)-encoding plasmid in 400 µL of Opti-MEM (Thermo Fisher Scientific, Waltham, MA, USA). After a 1-day culture ...
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bioRxiv - Neuroscience 2022Quote: ... Transfections with GPCR/pcDNA3.1 plasmids were performed when cells reached 70-80% confluency using Lipofectamine® LTX with Plus™ Reagent (Invitrogen) in serum-free medium ...
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bioRxiv - Developmental Biology 2022Quote: ... The ubi:urp2 and ubi:STOPlox-urp2 tol2 transgenesis vectors were produced using plasmids from the tol2kit (Kwan et al., 2007) and Gateway recombination system (Life Technologies). To produce the entry plasmid p5E-ubi:STOPlox (pCM351) ...
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bioRxiv - Cancer Biology 2022Quote: ... LCV2-GFP or LCV2-RFP were mixed with sPAX2 and MD2.G plasmids and transfected in HEK293 cells using Lipofectamine 2000 (Life Technologies). After 24h ...