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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... the neurons were given 12 hours in growth media at 37°C/5% CO2 to allow sufficient recovery and expression of the plasmid prior to fixation in 4% formaldehyde (Thermo Fisher) / 4% sucrose (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... Expression plasmid of HyPer7 was designed based on reported mammalian expression plasmid for HyPer7 (Pak et al. 2020) and was synthesized by Thermo Fisher Scientific with codon optimization for gene expression in C ...
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bioRxiv - Neuroscience 2023Quote: ... Transient transfections were accomplished with Lipofectamine LTX using 5 μg plasmid DNA per million cells in Opti-MEM reduced serum media according to manufacturer’s instruction (ThermoFisher, Waltham, MA). After transfection ...
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bioRxiv - Neuroscience 2024Quote: ... Both HEK cells and astrocytes were transfected the day after plating with 500 ng of plasmid DNA using lipofectamine 2000 (Invitrogen #11668019) and OptiMEM (Life Technologies #31985-070) ...
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bioRxiv - Cell Biology 2024Quote: ... add-back DLD1 cells were induced with doxycycline (1µg/mL) and 48 hr later transiently transfected with the construct of interest cloned into the pcDNA5 FRT/TO plasmid (Life Technologies). 48 hr after the transient transfection ...
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bioRxiv - Genomics 2024Quote: ... Each well of cells was transfected with the indicated plasmids and 4 µL each of Lipfectamine 3000 and P3000 reagent (Invitrogen #L3000015), suspended in 250 µL Opti-MEM (Gibco #31985062) ...
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bioRxiv - Developmental Biology 2024Quote: ... and in-vitro transcription using the Tol2 transposase plasmid was performed using the mMESSAGE mMACHINE SP6 kit (Invitrogen, Waltham, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... The cells were then transfected with the BFP reporter plasmid containing CAPG and CAPZA3 targeting gRNAs using Lipofectamine 3000 (ThermoFisher # L3000008). 2 days after transfection the cells were sorted and plated as individual cells into each well of 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were isolated according to the manufacturer’s instructions using a Midi Spin Column Plasmid Kit (Geneaid) and sequenced using vector-specific forward and reverse primers on a 3130xl Genetic Analyser (Applied Biosystems). Transfection of Giardia trophozoites was performed as follows ...
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bioRxiv - Immunology 2024Quote: ... a kind gift from Jason McLellan) was expressed by transiently transfecting transiently transfecting plasmid using FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 H2B-GFP/mRFP-α- tubulin/Halo-Tag9-LaminB1 cell line was generated by transiently transfecting a HaloTag9-LaminB1 plasmid (gift from Tom Misteli) using Lipofectamin 2000 (Life Technologies). Specifically ...
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bioRxiv - Plant Biology 2023Quote: ... previously obtained by in vitro transcription from plasmids containing the individual genomic sequences using the MAXIscript T7 Transcription Kit (Thermo Fisher). N ...
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bioRxiv - Cancer Biology 2024Quote: ... lentiviral vector DNA was transfected with psPAX2 and pMD2.G packaging plasmids into HEK-293FT cells using Lipofectamine 2000 transfection reagent (Invitrogen, 11668019) and D10 media lacking antibiotics ...
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bioRxiv - Cancer Biology 2023Quote: ... recombination was achieved by transfecting the acceptor cells with the KIF18A plasmid and recombinase using an LTX transfection (Thermo Fisher Scientific). Recombination was initially selected for with 1 μg/mL puromycin for 48 hours followed by a stricter selection with 2 μg/mL puromycin for 48 hours prior to switching back to 1 μg/mL puromycin for HeLa Kyoto cell lines ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA encoding either the CHIKV (pSP6-ICRES1) or DENV-2 strains (pFK-DVs) was linearised using FastDigest NotI (Thermo Fisher) or XbaI (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... XbaI linearized plasmid templates were used to make mRNA for injection using the Ambion mMessage mMachine T7 mRNA Synthesis Kit (ThermoFisher AM1344) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Plasmid containing a codon-optimized sCD4 gene was transfected into 293F cells using 293fectin transfection reagent (Invitrogen, Thermo Fisher Scientific Inc.). Transfected cells were grown for 3-6 days at 37°C and 8% CO2 with continuous shaking and the secreted proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... were cloned into the Gateway pENTR plasmid using Gibson Assembly and recombined using the Gateway LR Clonase II Enzyme mix (Life Technologies). Resultant plasmids were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research) ...
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bioRxiv - Biochemistry 2022Quote: ... Cac1 mutants from the pDONR/Zeo plasmids were then sub-cloned into the pDEST8 vector via Gateway technology (Thermo Fisher Scientific) followed by Sanger sequencing verification (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200μg of plasmid DNA was transfected into 200 ml of Expi293F cells (3.0 x 106 cells/ml) using Expifectamine (#A14524, Thermo Fisher Scientific). Cells were cultured in Expi293 expression medium (#A14351 ...
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bioRxiv - Immunology 2023Quote: ... The absolute number of gene copies was calculated using a plasmid DNA standard curve and the results were normalized to levels of Gapdh (Applied Biosystems). The relative quantification of X31 M genes (primers from Ward et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Co-transfection of CMV-GFP construct and plasmids packaged with pCMV-vsvg and pCMV-gp was achieved using lipofectamine 3000 (Life Technologies). The medium of transfected cells was collected and concentrated by ultracentrifugation (20,000 for two hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Large-scale preparation of pCARGO-RMCE-Airn plasmid was purified from cells grown overnight at 30°C with ampicillin selection (100μg/mL ampicillin sodium salt, Fisher Scientific BP1760), using the NucleoBond BAC100 Maxiprep Kit (Macherey-Nagel 740579) ...
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bioRxiv - Microbiology 2023Quote: ... BSF cells and a T7 polymerase/Cas9 expressing derivative line (derived using plasmid pJ1339)128 were cultured in HMI-9 medium (Life technologies), supplemented with 10% foetal calf serum at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... containing an ORF of interest and retroviral packaging plasmids (VSV-G and Gag-Pol) were transfected into HEK293T cells using Lipofectamine LTX (Life Technologies) for 15 h ...
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bioRxiv - Plant Biology 2023Quote: ... The purified Pdonr223-NtFP plasmid was used to clone NtFP into the binary vector PMDC84 with Gateway LR Clonase II Enzyme Mix (Invitrogen, USA) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... full-length infectious molecular clone plasmid DNA (originally obtained from Dr John Kappes, University of Alabama at Birmingham, USA67) was transfected using Lipofectamine (Life Technologies) or Fugene 6 (Promega ...
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bioRxiv - Biophysics 2023Quote: 12-well cultures of Hep-2 cells at 70% confluence were transfected with 0.5 μg of wild-type or mutant UL34 or UL31-FLAG plasmid using Lipofectamine as described by the manufacturer (Gibco-BRL) and incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... the HEK293T-MMTV cells were transfected with the appropriate plasmids (listed in Table 1) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific, USA), as described by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... the HEK293T-MMTV cells were transfected with the appropriate plasmids (listed in Table 1) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific, USA), as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected with relevant expression plasmids as described in the figure legends using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown to 70% confluency in 24-well plates and transfected with 250 ng of indicated plasmids using Lipofectamine LTX and Opti-MEM (both from Life Technologies), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were transfected with plasmid vectors expressing λN-HA-BASU and one of the BoxB-RNA fragments using LipofectamineTM 3000 transfection reagent (L3000001; ThermoFisher Scientific), according to vendor’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Human pDEST47-MCU-GFP plasmid (1.5 µg) was overexpressed in B16 cells plated at 60% confluency using Lipofectamine 2000 (Invitrogen, 11668-019). After 18 hours post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... MEFs were transfected with the isolated N-TAP progranulin plasmid (courtesy of the UF CTRND) using Lipofectamine LTX Reagent with PLUS Reagent (Invitrogen 15338030) according to its included protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... TALEN mRNAs were in vitro transcribed from SacI-linearized expression plasmids with T3 RNA polymerase using a mMessage mMachine mRNA kit (Thermo Fisher). Embryos ...
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bioRxiv - Cancer Biology 2023Quote: ... The B6-LSL-Cas9-eGFP keratinocytes were transiently transfected with 1μg of a CRE-expressing plasmid in 6-wells using Lipofectamine 2000 (Invitrogen; 11668-027) to activate Cas9-GFP ...
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bioRxiv - Developmental Biology 2023Quote: ... These inserts were introduced into the pMPRA1 Addgene plasmid backbone (no. 49349) (Melnikov et al., 2014) via Gibson assembly and transformed into ElectroMAX Stbl4 Competent Cells (ThermoFisher, 11635018) using 1.8 kV ...
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bioRxiv - Molecular Biology 2023Quote: ... sub-confluent HEK293T cells in 24-well plates were transfected with 500 ng of the psiCHECK-2 reporter plasmids mentioned above (G4Q27, G4M27, and native psiCHECK-2) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Neuroscience 2023Quote: ... The tol2 transposase mRNA was in vitro transcribed from the NotI-linearized pCS-TP6287 plasmid (gift from Wilson lab, UCL) using an SP6 mMESSAGE mMACHINE Kit (Ambion, USA). RNA was purified using RNA Clean & Concentrator Kits (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... and 3 µg of a plasmid expressing the WT or mutant C-prM-E structural genes of interest using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with a mixture of 3 µg of the lentiviral plasmid containing the gene of interest and 9 µg of the ViraPower Packaging Mix using LipofectamineTM 2000 (ThermoFisher # 11668030). At 72 hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 1.5 ug of WT or mutant SARS-CoV-1 S plasmid using polyethylenimine (PEI) (Thermo Fisher Scientific). Cells were transfected for 24 hours ...
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bioRxiv - Systems Biology 2023Quote: ... A 6-well plate was co-transfected with 0.5 µg of the pEXPR plasmid and 1 µg of the pOG44 Flp-recombinase expression vector (Thermo Fisher Scientific) using 4.5 µg of PEI (Polysciences ...
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bioRxiv - Neuroscience 2023Quote: 1×106 ES cells were electroporated with 2.5 µg of the cloned pX330-Puro-C9orf72 plasmid and 2.5 µg of targeting vector using the Neon Transfection System (Thermo Fisher Scientific) (3 × 1400 V ...
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bioRxiv - Molecular Biology 2023Quote: ... cRNA was prepared according to the manufacturer’s instructions from linearized plasmids encoding SARS-COV2 Protein E using the mMESSAGE mMACHINE T7 kit (Ambion, TX, USA). RNA concentrations were quantified by UV spectroscopy (NanoDrop ...
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bioRxiv - Microbiology 2023Quote: ... with 127 μg each of heavy and light chain expression plasmids for the respective antibodies (kindly provided by Peter Kwong, Vaccine Research Center, NIH) using 293fectin (ThermoFisher Scientific) transfection reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... A1/A2 dKO Raw 264.7 cells were maintained in chamber slides and plasmids were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) in presence of NATETM (InvivoGen) ...