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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and 1 µg gRNA plasmid were transfected into RPE-1 cells using the Lipofectamine 3000 reagent according to the manufacturer’s instruction (Invitrogen, Carlsbad, CA). Cells were expanded ...
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bioRxiv - Cell Biology 2021Quote: ... University of Western Australia) and plasmids for the NanoBiT complementation assays (see Supplementary Methods) were performed using Lipofectamine 2000 (Thermo Fisher) for HEK293T and Huh7 cells ...
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bioRxiv - Cell Biology 2022Quote: ... lines were generated in HEK293T cells expressing retroviral packaging plasmids (gift from N. Montserrat) and transfected using Lipofectamine™ 3000 Transfection Reagent (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... A plasmid encoding KIF22-GFP T134A for recombination was generated using Gibson Assembly of a synthesized DNA fragment (Thermo Fisher Scientific) and pAT4250 ...
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bioRxiv - Cell Biology 2022Quote: ... Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco, 31985070) according to the Lipofectamine 2000 instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg of plasmid encoding SARS-CoV2 spike (D614) with C-terminal 18 amino acid deletion using Lipofectamine LTX Plus reagent (Invitrogen, USA) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: 1μg of Cas9 plasmid and 0.3μg of either gBlock were then cotransfected into MCF7 cells with Lipofectamine3000 (Invitrogen, Cat. No. L3000001) in a 6-well plate ...
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bioRxiv - Biochemistry 2022Quote: ... and co-transfected with wild type and mutant constructs of PKD 20 h post-seeding with 250 ng of wild-type and 250 ng mutant plasmid DNA per well using TurboFect (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR template cassette together with the enAsCas12a expression plasmid were electroporated into hTERT-RPE1 cells using the Neon Transfection System (Thermo Fisher), and cells were selected with zeocin or puromycin for 2 weeks ...
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bioRxiv - Immunology 2022Quote: ... and H1 SI06 (A/Solomon Islands/3/2006) were produced by transient transfection of the corresponding plasmid(s) in Expi293 cells (Life Technologies) using Expifectamine293 transfection kit (ThermoFisher Scientific ...
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bioRxiv - Physiology 2022Quote: In vitro transcription from the gRNA template or a Cas9 plasmid (T7-Cas9-HA-2NLS) was performed to produce gRNA or Cas9 mRNA using mMESSAGE mMACHINE T7 ULTRA kit (Invitrogen, AM1344). The ssODN stock solution was prepared according to manufacturer’s recommendation (IDT Technologies) ...
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bioRxiv - Physiology 2022Quote: ... Success of the transformation and choice of the recombinant plasmids was verified in LB agar cultures (Lennox L Agar, Thermo Fisher) containing Ampicillin (200 mg/mL ...
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bioRxiv - Microbiology 2022Quote: HK1 grown in 100 mm plates were transfected with 5 μg of RFP-LMP1 or GFP-PD-L1 plasmid using Lipofectamine 3000 transfection kit (Invitrogen, L3000015). Lipofectamine reagents were diluted in Opti-MEM medium (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 500 ng of plasmid encoding the indicated protein and 1.5 μL of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids with correct gRNA sequences or empty vector control were transfected into KPFC cells with Lipofectamine 2000 (11668027, Thermo Fisher Scientific). Positive cells expressing GFP were sorted as single clones and expanded ...
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bioRxiv - Biophysics 2022Quote: 106 HEK cells were plated in 6-well plates and transfected with the indicated plasmids with Lipofectamine 2000™ reagent (Invitrogen). 24 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... genes and linear plasmid vectors were amplified by PCR reaction using the Phusion™ High-Fidelity DNA Polymerase (Thermo Scientific™) and the appropriate primers following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Biophysics 2022Quote: ... 1 μg of NPY-td-mOrange2 plasmids were co-transfected with 20 μl prepared SN25 knockdown or rescue lentiviruses by using LipofectAmine 3000 (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... DHIV3-GFP and env-expression plasmids were co-transfected at a mass ratio of 2:1 using lipofectamine 2000 (ThermoFisher, 11668030). shRNA encoding lentiviral particles were produced by co-transfecting pHIV7 ...
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bioRxiv - Developmental Biology 2022Quote: ... To obtain capped nid1b-GFP fusion mRNA for injections the plasmid was linearized with NotI and transcribed using the SP6 mMessage Machine Kit (Ambion/ThermoFisher). 2.5 nl nid1b-GFP mRNA was injected at a concentration of 80 ng/µl alone or together with 1 ng/µl nid1b-ATG-MO ...
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bioRxiv - Developmental Biology 2022Quote: ... The obtained fragment was cloned into the StuI and XhoI sites of the pCS2+ expression vector and the resulting plasmid was linearized with NotI and transcribed using the SP6 mMessage Machine Kit (Ambion/ThermoFisher). 2.5 nl mNidogen-2 mRNA was injected per embryo at concentrations of 200 ng/µl alone or together with 1 ng/µl nid2a-1.ATG-MO.
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bioRxiv - Biochemistry 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... After one day in culture cells were transfected with a mix of plasmid DNA using 250 μl Opti-MEM® I reduced serum medium and 10 μl LipofectamineTM 2000 (Invitrogen) per well ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with plasmid DNA following mixing with 50 μl Opti-MEM® I (reduced serum medium) and 2 μl LipofectamineTM 2000 (Invitrogen). For Western blotting and coimmunoprecipitation experiments ...
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bioRxiv - Cell Biology 2022Quote: ... These cells were then transfected with 6 μg of pIRES-AcGFP-FLAG-Ect2r or pIRES-AcGFP-FLAG-Ect2rΔmem plasmid using 12 μl of lipofectamine 2000 (Life Technologies, 11668019). After 36 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... and a mix of the three pGIPZ-shNSMCE2 specific constructs or the pGIPZ-shControl plasmid using TurboFect Transfection Reagent (Life Technologies). Lentiviral particles were harvested 48 hours after transfection in the culture media and used to infect target cells ...
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bioRxiv - Cell Biology 2022Quote: ... p3E-EGFP-pA (gift from Rodney Steward) and pDestpBHR4R3 (gift from Susan Brockerhoff) to generate mpeg1: WT/Y118E/Y118F-pxna-EGFP plasmids by LR clonase (Thermofisher, 11791020).
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 9µg of pCMV-SARS2-RRAR_ILR_gp41 and 29µg of pCMV-Lenti-Luc plasmids were mixed in 3.6mL of Opti-MEM™ I Reduced Serum media (Gibco, Cat# 31985070) along with 144 µL of PEI Max 40K (1 mg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Zebrafish codon-optimised Tol1 transposase mRNA was prepared by in vitro transcription from NotI-linearised pCS2-Tol1.zf1 plasmid using the SP6 transcription mMessage mMachine kit (Life Technologies). The pCS2-Tol1.zf1 was a gift from Harold Burgess (Addgene plasmid # 61388 ...
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bioRxiv - Neuroscience 2020Quote: ... Zebrafish codon-optimised Tol2 transposase mRNA was prepared by in vitro transcription from NotI-linearised pCS2-zT2TP plasmid using the SP6 transcription mMessage mMachine kit (Life Technologies). The pCS2-zT2TP was a gift from Koichi Kawakami (Suster et al. ...
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bioRxiv - Microbiology 2021Quote: ... For preparation of mutated protein this plasmid was mutated at codon 119 using the Phusion Site-Directed Mutagenesis kit (Thermo Scientific). PCR reactions were set up with Phusion HSII polymerase ...
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bioRxiv - Developmental Biology 2021Quote: ... Linearized plasmid templates were used to make mRNA for injection using the Ambion mMessage mMachine SP6 RNA synthesis kit (ThermoFisher AM1340). Total amounts of injected mRNA were as follows ...
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bioRxiv - Developmental Biology 2020Quote: ... Transgenic lines were generated by injecting 15 ng/µl of either plasmid pSMR10 or pSMR30 together with 65 ng/µl lambda DNA (Thermo Scientific) into the gonads of young adults.
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bioRxiv - Developmental Biology 2020Quote: ... 3 μl of the lysate was mixed with primers and probes detecting the excision product and a gene on the input plasmid in a total reaction volume of 20 μl using TaqMan Universal PCR Master Mix (Applied Biosystems) on the 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: Huh-7 cells were transfected with the HBsAg or luciferase reporter derived plasmids per manufacturer’s instructions (Lipofectamine 3000, Invitrogen, MA, USA). Cells were treated with the indicated compounds for 5 days ...
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bioRxiv - Biophysics 2020Quote: ... 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher). Transformants were selected on Zeocin containing YPDS plates at a Zeocin concentration of 2mg/mL (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 293FT cells were then co-transfected with lentivirus packaging plasmids (lenti-CRISPR v2-DHODH-sgRNA, psPAX2, and PMD2.G) by Lipofectamine® 2000 (ThermoFisher) for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... New York University) and a pcDNA3.1 plasmid expressing the SARS-CoV2 glycoprotein into HEK 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). The supernatant was collected 48 h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... The virus stocks were prepared from the conditional medium after transfection of plasmid DNA into 293FT cells (Lipofectamine™ 3000, Cat. No. L3000015, Invitrogen), and then concentrated using the lenti-x concentrator (Cat ...
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bioRxiv - Microbiology 2021Quote: ... (VEEV-TC-83-GFP or nLuc) was transcribed in vitro from cDNA plasmid templates linearized with MluI via MegaScript Sp6 kit (Invitrogen #AM1330) and electroporated into BHK-21 cells ...
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bioRxiv - Biochemistry 2021Quote: ... and each expression plasmid (1 μg, pcDNA3.1/ERβ or pcDNA3.1/ERβ (W335A)) using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific, Inc.), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... DNA templates were amplified from monocistronic pGL3 plasmids using a SP6-flanked forward primer and Fluc-specific reverse primer (KL414/KL415) and the MEGAscript SP6 kit (Ambion, AM1330), as described in the 4xS1m pulldown section ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.5 µg of the piggyBac transposase plasmid pCyL43 (16) were co-transfected to each well using the Lipofectamine 2000 reagent (Thermo Fisher). These cells were then selected using 2 µg/mL puromycin and 200 µg/mL G418 for two weeks ...
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bioRxiv - Biochemistry 2020Quote: ... containing 300 µg of PB-CMV plasmid DNA was mixed with 15 mL Opti-MEM medium containing 400 µL 293fectin reagent (Thermo Fisher). The mixture was incubated for 5 minutes before being added to the shaker flask ...
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bioRxiv - Molecular Biology 2022Quote: ... The sequencing ladder was obtained on a plasmid containing the 9S coding gene (rrfB) using USB® Sequenase ™ version 2.0 DNA polymerase (Affymetrix) following the supplier’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR product was cloned into the Age1 and BamHI sites of pLVX-TetONE-puro plasmid using T4 DNA ligase (Thermo Scientific). CYB5B-HA ...
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bioRxiv - Immunology 2022Quote: ... pAb20-hCHIgG1 and pAb20-hCK plasmids discretely expressing heavy and light chain of antibodies were transiently co-transfected into Freestyle-293F cells (R79007, Thermo Fisher) employing purefection reagent (LV750A-1 ...
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bioRxiv - Immunology 2022Quote: ... pAb20-hCHIgG1 and pAb20-hCK plasmids separately expressing heavy and light chain of antibodies were transiently cotransfected into Freestyle-293F cells (R79007; Thermo Fisher) using purefection reagent (LV750A-1 ...