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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... in forward and reverse orientations were cloned into a pENTR vector and plasmids used for site-specific chromosomal inversion were generated by Gateway cloning (ThermoFisher Scientific) of yeast recombination sites into introns of fluorescent reporter genes.
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bioRxiv - Genetics 2019Quote: HEK cells were transfected with plasmids encoding GFP-tagged Cdc42 variants using Calcium Phosphate transfection or Lipofectamine LTX Kit (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... and 4 μL was mixed with 1 μL Salt solution (1.2M NaCl, 0.06M MgCl2) and 1 μL pBAD-TOPO® plasmid (Invitrogen, K4300-01) and incubated for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... E14Tg2a-rtTAms2 cells were then transfected with piggyBac-TetON-Nanog-PGK-Hph-pA or piggyBac-TetON-Esrrb-PGK-Hph-pA together with pCMV-hyPBASE (Yusa et al., 2011) plasmids using lipofectamine 3000 (Thermofisher; L3000015).
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bioRxiv - Evolutionary Biology 2020Quote: ... Plasmid DNAs were isolated according to standard protocols and recombinant plasmids were subjected to sequencing using universal M13 primers and the BigDye terminator cycle sequencing kit v1.1 (Thermo Fisher Scientific). Sequencing products were EDTA/ethanol-precipitated ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Subcloning into the pSP64t expression plasmid was accomplished using the Gateway® LR clonase II® enzyme (Life Technologies, Carlsbad, CA). Individual cRNAs were synthesized in vitro from linearized pSP64t using the mMESSAGE mMACHINE® SP6 kit (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... We co-injected into one-cell stage zebrafish embryos 15 pg of UAS:pcdh18aDCDREDV/AEAV-GFP construct with 25 pg of tol2 transposase mRNA, transcribed from linearized pCS-zT2TP plasmid (Suster et al., 2011) using the mMESSAGE mMACHINE SP6 kit (Ambion, AM1340). Embryos expressing GFP were sorted and imaged at the desired stages.
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bioRxiv - Immunology 2022Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following manufacturer’s indications ...
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bioRxiv - Biochemistry 2022Quote: ... and pMES4-Nanobody expression plasmids described previously were transformed into and expressed using the One-Shot BL21 Star DE3 bacterial cell line (Invitrogen, C601003) by stimulating induction with 0.5 mM IPTG (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Over-expression vectors were prepared by cloning the PLAGL2 coding sequence into BII-ChBtW and IEC6 cells transfected in 6 well plates with 1600 ng of this plasmid and 400 ng of pCMV-hyPBase using Lipofectamine 2000 (ThermoFisher Inc.). Cells were select 48 hours after transfection with medium containing 4 µg/ml Blasticidin and selected for 5 days ...
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bioRxiv - Bioengineering 2022Quote: ... 150 ng guide RNA-encoding plasmid and 150 ng Cas13-encoding plasmid or 2 times 100 ng of each Split-Cas13-encoding plasmid was mixed with 25 μl Optimem and 0.5 μl Lipofectamine 2000 (Thermo Fisher #11668019), incubated at room temperature for 10 minuets and then pipetted onto the cells ...
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bioRxiv - Bioengineering 2022Quote: ... 0.4 μl P3000 and 100-300 ng plasmid DNA was pooled with 25 μl Optimem and 0.3 μl Lipofectamine 3000 (Thermo Fisher #L3000001), incubated for 10 minutes at room temperature and then pipetted onto the cells ...
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bioRxiv - Biochemistry 2022Quote: ... and transfected with 24 µg of plasmid encoding either the wildtype or E406W SARS-CoV-2 spike protein using Lipofectamine 2000 (Life Technologies). Four hours at transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... we cloned an EcoCas3 cDNA with a octa-histidine tag and a six asparagine-histidine repeat tag into a pFastbac-1 plasmid (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... targeted sequences of EMX1 with PAM variants (AAG or CCA) were cloned into a pCR4Blunt-TOPO plasmid vector (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The expression plasmids encoding HC and LC sequences were co-transfected into Expi293F cells using ExpiFectamine™ 293 Transfection reagents (Gibco). For IgG expression ...
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bioRxiv - Molecular Biology 2020Quote: ... CHO-K1 cells were plated in six-well plates at 0.5 × 106 cells/well and transfected with 1 μg of each indicated expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 mRNA for mouse zygote injection was in-vitro transcribed from the XbaI-linearized Cas9 plasmid with a T7 promoter using mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies). The sgRNAs were in-vitro transcribed from PCR products with the MEGAshortscript Kit (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg linearized plasmid DNA was used in a 30 µl reaction with the mMessage mMachine SP6 Transcription Kit (Invitrogen: AM1340). RNA integrity was examined on a 1% agarose gel ...
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bioRxiv - Molecular Biology 2020Quote: ... We also sequentially removed the 5’ and 3’ viroid moieties from this latter plasmid via PCR with the phosphorylated primers (T4 polynucleotide kinase, Thermo Scientific) D3606 and D3285 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3.5 μg of our BFP-UGI plasmid diluted to 1.5 ml with Opti-MEM I Reduced Serum Medium (Gibco, cat #31985088). The transfection solution was incubated for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... S2 cells grown in SFX medium were co-transfected by 3xFLAG-Pita (wild-type and with deletion of CP190-interacting region) and CP190 plasmids with Cellfectin II (Life Technologies), as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were transfected with indicated plasmids alone or in combination for 8 h by using Lipofectamine 3000 (Thermo Fisher, USA), and then continue to cultivate for 24 h in the fresh medium before being subjected to indicated experiments.
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bioRxiv - Molecular Biology 2021Quote: ... S2 cells grown in SFX medium were co-transfected by 3xHA-dCTCF (wild-type and with deletion of CP190-interacting region) and CP190 plasmids with Cellfectin II (Life Technologies), as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of the plasmid was digested with KpnI and BamHI in a 20 μL reaction for 1 h using FastDigest enzymes (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... In-house expressed antibodies were made through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... we seeded 2×105 HEK293FT cells per well of a 24-well plate prior to transfection (12 - 18 hours) and used 500 or 750 ng plasmid together with a 5-to-1 ratio of Lipofectamine 2000 (ThermoFisher 11668019) or 1 mg/mL polyethylenimine (Polysciences 23966 ...
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bioRxiv - Genomics 2019Quote: ... All six pX300-gRNA plasmids were pooled and 1 µg was transfected into 2 million E14tg2a cells using Lipofectamine (Life Technologies). A separate pX300 empty vector was also transfected into E14tg2a to serve as a negative control ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep seeded in 10-cm dishes were transfected with the plasmid encoding for the corresponding S glycoprotein using lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 1,000,000 wild-type (J1) mESCs were transfected with 1µg of each 5’ and 3’ sgRNA-Cas9-mCherry plasmids using Lipofectamine 2000 (Thermo Fisher Scientific). After 24hrs of transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... served to ligate the purified inserts into pCAGGS/MCS plasmid (34) purified from agarose gel by the GeneJET Gel extraction kit (ThermoFisher Scientific) after linearization with Fast Digest EcoRI and XhoI (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293T cells in a 10 cm dish with about 60% confluence were transfected with 8 μg lentiviral construct and helper plasmids using Lipofectamine 2000 (Life Technologies). 24 hours after transfection ...
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bioRxiv - Biophysics 2019Quote: ... The plasmids were heat shocked into Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific, Cheshire, UK) and purified as previously described 18 ...
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bioRxiv - Systems Biology 2020Quote: ... with 125 ng SwaI/BclII-linearised pML104, (directing expression of Cas9, and the guide RNA) (29) and 70 ng of plasmid repair DNA fragment (Life Technologies, Table S1 ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were transfected with 2 μg of plasmid by electroporation using the Neon Transfection System according to the manufacturer’s instructions (Thermo Fisher Scientific). 48 hours after transfection cells were sorted based on GFP into 96-well plates at a single-cell-per-well density for clonal expansion ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T cells were grown in 10 cm dishes and transfected with APEX2-tagged mammalian expression plasmids using Lipofectamine 3000 (Thermo Fisher). Cells were washed off the plate 12-15 hours post-transfection with Dulbecco’s PBS and then pelleted at 500x g ...
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bioRxiv - Developmental Biology 2019Quote: ... we amplified the patient cDNA using PHF6-specific primers flanking the site of mutation and subcloned into a plasmid vector using TOPO-TA cloning (Thermo Fisher) and sequenced according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Virus was generated by transfection of HEK293FT cells with appropriate packaging plasmids (Lentivirus: pMD2.G and psPAX2; Retrovirus: pUMVC and pVSV-G) with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa Kyoto cells were transfected with two guide RNA and one HDR donor plasmid (or 4 guide RNA and two HDR donor plasmids for the EGFP-STAG1 FLAG-mRFP-STAG2 double tagged cell line) using Lipofectamine 2000 (Invitrogen 11668019). Cells were grown for 7-10 days before sorting single cells into 96 well plates ...
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bioRxiv - Developmental Biology 2019Quote: The LacZ and white probes were generated by transcription from linearized pBluescript template plasmids (kindly provided by Dr. Mattias Mannervik) with T3 or T7 RNA polymerase (Thermo Fisher) and Dig RNA labelling mix (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... Cas9 mRNA for the injections was produced by in vitro transcription of the p3T3S-nls-zCas9-nls plasmid [39] using the T3 mMessage machine transcription kit (Ambion/Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... using 5 µL of in vitro methylated plasmid DNA or 1.5 µg genomic DNA measured with the Qubit dsDNA BR Assay (Thermo Fisher Scientific). 1 µL of bisulfite-converted DNA was amplified with HotStar Taq DNA polymerase (QIAGEN ...
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bioRxiv - Cell Biology 2019Quote: ... hTert-RPE1 cells were co-transfected for 24 h with pCMV-Cas9-RFP and pUC57-kan plasmids using Opti-MEM and Lipofectamine 2000 (Thermofisher Switzerland) following the provider’s instructions ...
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bioRxiv - Neuroscience 2019Quote: The TPBG expression vector contains full-length mouse TPBG cDNA (NM_011627.4) inserted into the pCMV-sport6 plasmid (Thermo Scientific; Cat# 12209) provided by the PlasmID Repository (Clone ID ...
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bioRxiv - Microbiology 2019Quote: ... Large scale transient transfections for murine CRD4-7-Fc and CR-Fc were carried out using 100 µg of total plasmid DNA and 100 ml of suspension cultured Expi293F cells (Life Technologies). Supernatants were harvested at day 6 ...
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bioRxiv - Immunology 2019Quote: DNA plasmids (Caspase-1-GFP and NOD1-mOrange) 59 or specific silencer and negative control siRNA (40 μmol of each; ThermoFisher Scientific) were diluted in Opti-MEM medium (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Entry clones of myosin XI (WT and mutants) and 3mEGFP were cloned into a moss expression plasmid with LR clonase (Thermo Fisher), yielding pB1-MyoHNC-B5-gtail-mutation-B5-3mEGFP-B2 ...
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bioRxiv - Molecular Biology 2019Quote: ... LB was added to the sorted cells and they were grown at 37°C for 2 hours prior to plasmid isolation using PureLink HiPure Miniprep Kit (Thermo Fisher).
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bioRxiv - Genomics 2020Quote: ... Trono) and 13.6 µg target library plasmid were mixed in 651 µL Opti-MEM (serum free, Opti-MEM (Thermo Fisher Scientific)) ...
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bioRxiv - Developmental Biology 2019Quote: The following chick plasmids were used to generate Digoxigenin–labeled antisense probes: Etv4 (a kind gift from M. Bronner) Spry1 obtained from Life Technologies Klf7 ChEST376015 ...