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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: In pcDNA3.1-TRIP6-HA the mouse Trip6 CDS with a C-terminal HA epitope was cloned by PCR between the NheI and EcoRI sites of pcDNA3.1 (Invitrogen, Karlsruhe, Germany). The KpnI / XhoI fragment of pcDNA3.1-TRIP6-HA ...
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bioRxiv - Molecular Biology 2024Quote: ... The primers (ThermoFisher Scientific) were as follows (5’ to 3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... Blots were imaged and quantified using iBright CL1500 Imaging System (Thermo Fisher Scientific Inc., Waltham, MA).
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bioRxiv - Molecular Biology 2024Quote: ... Invitrogen) and loaded onto an 8% native acrylamide gel and visualised with SYBR green (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... 99+% (Thermo Scientific Chemicals). Immediately following stimulation ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). After protein quantification ...
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bioRxiv - Cancer Biology 2024Quote: ... Ba/F3 cells expressing wild-type or mutant CD74-ROS1 constructs were seeded at 1,000 cells per well in a volume of 25 μl using a Multidrop Combi Reagent Dispenser (Thermo Fisher Scientific). Plates were incubated for 72 hours at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The extraction of total RNA from the tissues was carried out using TRIzol (Invitrogen, Carlsbad, CA, USA) under the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... lysates were extracted with Laemelli sample buffer for 10 minutes at 75°C and lysates were run on 4% to 12% Bis-Tris gels (Invitrogen; Thermo Fisher Scientific). Proteins were transferred to nitrocellulose membranes (Prometheus ...
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bioRxiv - Cancer Biology 2024Quote: ... NP1542 and 22RV1 cells were grown in RPMI medium with 10% FCS (Invitrogen). Cells were transiently transfected with the indicated plasmids using Lipofectamine 2000 or 3000(Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The Agilent 2100 bioanalyzer and NanoDrop were then used to quantify total RNA (Thermo Fisher Scientific, MA, USA). The construction of the mRNA library ensued ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transiently transfected with the indicated plasmids using Lipofectamine 2000 or 3000(Invitrogen) as per manufacturer’s instruction.
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bioRxiv - Molecular Biology 2024Quote: ... coupled to an orbitrap mass spectrometer (Q-Exactive Hybrid Quadrupole-Orbitrap, Thermo Fisher Scientific, USA). The mobile phase solvent A was water with 0.1% formic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... This was done on an Orbitrap-Elite hybrid ion trap MS instrument (Thermo Fisher Scientific GmbH, Bremen, Germany) running in data-dependent acquisition (DDA ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was reverse transcribed using Superscript II Reverse Transcriptase (Life Technologies) with random primers (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Click-IT Alexa594 (Life Technologies) reactions were performed as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Generated cDNA was used as template for quantitative Real-Time PCR using an Applied Biosystems (Life Technologies) Fast 7500 thermal cycler ...
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bioRxiv - Molecular Biology 2024Quote: ... The eyes were macerated in TRIzol and the RNA was purified using the PureLinkTM RNA Mini Kit (ThermoFisher Scientific, Waltham, MA, USA) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The following primary antibodies were applied to detect the expression of iPSC markers: anti-TRA-1-60 (Invitrogen, 41-1000, 1/100), anti-SSEA4 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:50,000 diluted Hoechst (Thermo Fisher Scientific, H1398) was added to stain nuclei for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-SSEA4 (Invitrogen, 41-4000, 1/200), anti-NANOG (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... The stable INTS6-GFP and hSSB1-GFP mutants were generated in HeLa WT with Lipofectamine LTX (Invitrogen, 15338100) transfection (2μg plasmid ...
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bioRxiv - Molecular Biology 2024Quote: ... Mass spectrometric data were acquired using an Orbitrap mass spectrometer (Q Exactive, ThermoFisher). HDX analyses were performed in triplicate from single preparations ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were extensively washed using TBST between each incubation and sealed on the slide glass using ProLong™ Diamond Antifade Mountant (Thermo Fisher Scientific, Cat# P36965). For primary antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... IgG and H1.8 antibody beads were crosslinked using 4 mM BS3 (Thermo Fisher Scientific, # A39266) resuspended in PBS (pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Volume visualization was performed in Avizo 2020.2 (Thermo Fisher Scientific). Data were reported in the standard format used by The American Society for Bone and Mineral Research (ASBMR ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µL of nucleosome fractions were mixed with 1 μL of 10 mg/mL RNaseA (Thermo Scientific) and incubated at 55 °C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and rabbit anti-H1.8 custom antibodies (26) (Identification# RU2130) were conjugated to Protein-A coupled Dynabeads (Thermo Fisher Scientific, # 10001D) at 250 μg/ml beads at 4 °C for overnight on a rotator ...
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bioRxiv - Molecular Biology 2024Quote: ... The vitrified grids were loaded onto the Titan Krios (ThermoFisher), equipped with a 300 kV field emission gun and a K3 direct electron detector (Gatan) ...
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bioRxiv - Molecular Biology 2024Quote: The vitrified grids were loaded onto the Titan Krios (ThermoFisher), equipped with a 300 kV field emission gun and a K3 direct electron detector (Gatan) ...
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bioRxiv - Molecular Biology 2024Quote: ... laevis H1.8 (# RU2130) were conjugated to Protein-A coupled Dynabeads (Thermo Fisher Scientific, # 10001D) at 20 μg/mL beads at 4 °C for overnight on a rotator ...
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bioRxiv - Molecular Biology 2024Quote: ... rat-specific primers were purchased (Thermo Fisher Scientific), including primers for 18S and GAPDH ...
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bioRxiv - Molecular Biology 2024Quote: ... on a 7900HT Real-Time PCR instrument (Thermo Fisher Scientific, 10966). The housekeeping gene Actb was used as the reference ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell nuclei were also stained with Hoechst 33342 Solution (Thermo Fisher Scientific). To quantify percentages of caspase 3-expressing areas ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1× penicillin/streptomycin (Thermo Fisher Scientific) at 37°C in 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... and bone metastatic BoM-1833 breast cancer cell lines (kindly provided by Joan Massague) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific), 0.02 mM folic acid (Millipore Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: NK-92 cells (ATCC; CRL-2407) were cultured in Minimum Essential Medium α (_-MEM) without ribonucleosides media (Thermo Fisher Scientific) supplemented with 12.5% fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were cultured in DMEM/F12 media (Thermo Fisher Scientific) supplemented with 5% horse serum (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor 488-conjugated goat anti-rabbit and Alexa Fluor 568-conjugated anti-rat antibodies (Invitrogen). Cell nuclei were also stained with Hoechst 33342 Solution (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1x penicillin/streptomycin (Thermo Fisher Scientific) at 37°C in 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5% horse serum (Thermo Fisher Scientific), 20 ng/mL EGF (Pepro Tech) ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were identified using tandem MS (MS/MS) with an Orbitrap mass spectrometer (Q Exactive, ThermoFisher). Spectra were acquired in data-dependent mode with the top five most abundant ions selected for MS2 analysis per scan event ...
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bioRxiv - Molecular Biology 2024Quote: ... The concertation of H1.8-GFP bound nucleosome in each fraction is calculated based on the 260 nm light absorbance detected by Nanodrop 2000 (Thermo Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mM L-Glutamine (Gibco), maintaining a density between 1 x 105 and 1 x 106 cells/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Nonspecific antibody binding was blocked using Ultravision Protein-block (Thermo Fisher Scientific). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... SXO viability was assessed using the ReadyProbe Cell Viability Kit (Invitrogen R37610). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were cultured in suspension using RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 U/ml penicillin-streptomycin (Gibco) and 1 mM L-Glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco), 100 U/ml penicillin-streptomycin (Gibco ...