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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Subsequent selection involved serial dilutions in Opti-MEM medium containing Geneticin (Thermo Fisher Scientific, 10131035), ranging from 200-400 ug/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and the aforementioned plasmid in Opti-MEM medium (Thermo Fisher Scientific). This mixture was allowed to interact at RT for 5 minutes prior to application ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were resuspended in 100 μL staining solution containing 1:1000 SYBR Green I (Invitrogen) dilution in 0.5% BSA/PBS and incubated for 20 mins at RT ...
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bioRxiv - Cell Biology 2024Quote: ... containing 10% heat-inactivated FBS and penicillin (100 IU/ml)/streptomycin (100 μg/ml) (Invitrogen) in a humidified 5% CO2 incubator at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... 10% vol/vol FBS (Invitrogen). After one hour of staining ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% vol/vol Penicillin-Streptomycin (Gibco, Cat #15140-148) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μg/mL streptomycin (Invitrogen), 10% vol/vol FBS (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... 1.0 mm denaturing gels (#XP10200BOX, Invitrogen, Waltham, MA, USA). After all protein constituents were separated by gel electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... The Click-IT Plus EdU Cell Proliferation Kit with Alexa Flour 488 and 647 (C10633, C10635 Invitrogen) was applied to cells for 30 minutes to detect EdU.
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bioRxiv - Microbiology 2024Quote: ... and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the Ares DNA labeling kit (Molecular Probes, Eugene, OR, USA) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... and the 96-well plate was centrifuged for 1 min at 1000 x g and subsequently run at the standard 20 µl qPCR SYBR green program on QuantStudio 3 real-time system (Applied Biosystem, ThermoFisher Scientific, Waltham, MA, USA). The experiment was performed as technical triplicates for each primer pair for each sample ...
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bioRxiv - Microbiology 2024Quote: ... RNA to cDNA reverse-transcription was performed using Maxima Reverse Transcriptase (Thermo Fisher Scientific) with Oligo (dT)18 primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems, Foster City, CA, USA) in a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: 6×104 NHF cells per well were plated in 24-well tissue culture plates and transfected using lipofectamine RNAiMax transfection reagent (ThermoFisher scientific, Waltham, MA, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of the appropriate cDNA was added and the 96-well plate was centrifuged for 1 min at 1000 x g and subsequently run at the standard 20 µl qPCR SYBR green program on QuantStudio 3 real-time system (Applied Biosystem, ThermoFisher Scientific, Waltham, MA, USA). The experiment was performed as technical triplicates for each primer pair for infected and non-infected samples ...
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bioRxiv - Microbiology 2024Quote: ... 1 volume of phenol:chloroform:isoamylalcohol (25:24:1, v/v, 15593031, Invitrogen, USA) was added ...
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bioRxiv - Microbiology 2024Quote: ... RNA obtained was treated with 10x TURBO DNase (Life Technologies, Carlsbad, USA) for 1 h to eliminate possible DNA contamination ...
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bioRxiv - Cell Biology 2024Quote: ... samples were counter stained with Hoechst (1:1000, ThermoFisher, 62249). Slides were cover slipped with Vectashield (Vecto Labsr ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS then counterstained with Hoechst (1:1000, ThermoFisher, 62249) for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of the appropriate DNA was added and the 96-well plate was centrifuged for 1 min at 1000 x g and subsequently run at the standard 20 µl qPCR SYBR green program on QuantStudio 3 real-time system (Applied Biosystem, ThermoFisher Scientific, Waltham, MA, USA). The experiment was performed as technical triplicates for each primer pair for infected and non-infected samples ...
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bioRxiv - Microbiology 2024Quote: ... abscessus and their combination on cell viability was analyzed by Presto Blue assay (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and run and analysed on the StepOnePlus™ real-time PCR system (Applied Biosystems). Full-length SMN2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µg total RNA from each sample was reverse-transcribed using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific™) according to the kit instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1x Concentrated sample reducing agent (Invitrogen), and NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... protein expression was carried out in HighFive cells (Thermo Fisher Scientific) at 130 rpm and 27 °C for 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... media supplemented with 10% FBS (Gibco 26140095), penicillin (10,000 IU mL-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... All flow cytometry was performed in 96-well format with the same Attune NxT Flow Cytometer (ThermoFisher) and analyzed using FlowJo ...
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bioRxiv - Molecular Biology 2024Quote: ... medium was aspirated and cells washed with PBS before addition of trizol (Ambion) and RNA was extracted using phenol/chloroform ...
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bioRxiv - Cell Biology 2024Quote: ... MitoTracker Deep Red (Invitrogen M22426, 2 nM) for 15 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... mouse hearts were isolated and washed with ice-cold HBSS 1x with 1 % penicillin-streptomycin (Gibco). Hearts were cut into halves ...
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bioRxiv - Cell Biology 2024Quote: ... a pre-plating dish was washed 3 times and DMEM supplemented with 10% FBS (Gibco) was added to the adhered cells and incubated at 37°C with 5% CO2.
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% FBS (Gibco), 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin-streptomycin (Gibco), insulin and FGF (Promocell)] ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM sodium pyruvate and 1% antibiotic-antimycotic (Gibco) at 37°C and 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (Gibco), 1 mM sodium pyruvate and 1% antibiotic-antimycotic (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin-streptomycin (Life Technologies) was added to the tube and the content was transferred to a falcon through a 70 µm cell strainer ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 mM L-glutamine (Gibco). icMEFs and iCMs (from day 1 post-transduction onwards ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines and primary fibroblasts were cultured in DMEM (Gibco) high glucose (4.5 g/L glucose ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin-streptomycin (Gibco), 0.1 mM norepinephrine (SIGMA ...
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bioRxiv - Cell Biology 2024Quote: ... containing protease and phosphatase inhibitors (Thermo Scientific). Cell lysates were then centrifuged at 15,100 g for 10 min at 4°C to remove insoluble cellular components ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were prepared by boiling with NuPAGE LDS sample buffer (Invitrogen) for 5 min at 95°C in the presence of 50 mM DTT and protein standard (NZYTech ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured using Pierce BCA Protein Assay Kit (Thermo Scientific) and a plate-reader Tecan Infinite M200 (Tecan) ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted using phenol/chloroform and quantified using the Qubit dsDNA Broad Range assay (Thermo Fisher Scientific). At least 20 µg of DNA were used for library preparation using the NEBNext Ultra DNA library prep kit ...
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bioRxiv - Molecular Biology 2024Quote: ... in nuclease-free water (Thermo Fisher).
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bioRxiv - Molecular Biology 2024Quote: ... 1 mL of stain buffer (ThermoFisher Sci 00-4222-57) was added and pipette mixed thoroughly ...
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bioRxiv - Molecular Biology 2024Quote: ... 32 µL of RevertAid RT Reverse Transcription Kit using random hexamer primers (Thermofisher) prepared using the manufacturer’s protocol was aliquoted into a separate 96-well PCR plate and 8 µL of the lysis samples were added and mixed ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 µL of stain buffer (Invitrogen) was added and mixed vigorously ...
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bioRxiv - Molecular Biology 2024Quote: ... media supplemented with 10% FBS (Gibco 26140095), penicillin (10,000 IU mL-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... growth medium was exchanged to DMEM without glucose (Gibco) 24 h after the second transfection and cells incubated for another 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were cultured in DMEM medium (Gibco, USA), supplemented with 10% fetal bovine serum (FBS ...