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Citations for Thermo Fisher :
9401 - 9450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... complementary DNAs was synthesized using SuperScript III reverse transcriptase (Thermo Fisher Scientific) and random hexamers in accordance with the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... the reaction mix was combined with 50 µL MyOne Streptavidin C1 Dynabeads (Life Technologies Cat#65001) in 2X B&W buffer (2 M NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Resazurin (alamarBlue; Invitrogen) (67 ...
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bioRxiv - Microbiology 2024Quote: ... were grown in Dulbecco’s minimal essential media (DMEM, Gibco, Thermo Fisher Scientific) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2024Quote: ... were grown in Dulbecco’s minimal essential media (DMEM, Gibco, Thermo Fisher Scientific) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2024Quote: ... Four micrograms of either pcDNA3.1 or pcDNA3.1-HA-LMP2A plasmid DNAs (provided by Prof. T. Kanda, Tohoku Medical and Pharmaceutical University) were transfected with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Samples of tissue were collected and placed into a solution of 10% neutral buffered formalin (Thermo Fisher Scientific) for 24 h ...
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bioRxiv - Microbiology 2024Quote: ... RIPA buffer (Thermo Fisher) was added along with a protease inhibitor (cOmplete mini ...
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bioRxiv - Microbiology 2024Quote: ... 200 U/mL penicillin and 200 mg/mL streptomycin (DMEM, Gibco, Thermo Fisher Scientific). Primary human Sertoli cells were purchased from and grown in Sertoli cell growth media (IX Cells Biotechnologies ...
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bioRxiv - Microbiology 2024Quote: ... 5’- UUUCCUUCCACUCGGAUAAGAUGCUGA-3’ were transfected with RNAiMaX (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... PI [10 µg/mL] (Life Technologies) was added ...
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bioRxiv - Microbiology 2024Quote: ... Laurdan (Invitrogen) was added to a final concentration of 10 µM and incubated at 37°C with rotation for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Zeocin solution was purchased from Invitrogen (Waltham, MA, USA. USA). Bleomycin sulfate powder (Tokyo Chemical Industry ...
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bioRxiv - Microbiology 2024Quote: ... Apoptotic cells were detected using a Dead Cell Apoptosis Kit (Thermo Fisher Scientific) and a Cytoflex flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2024Quote: ... The RNA concentrations were measured using a NanoDrop 2000 spectrophotometer (ThermoFisher). cDNA synthesis was performed using HiScript III RT SuperMix (Vazyme ...
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bioRxiv - Microbiology 2024Quote: ... donkey anti-goat Alexa Fluor 594 (1:2500, A32758, Invitrogen), was added ...
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bioRxiv - Microbiology 2024Quote: ... plasmids were linearized and in vitro transcribed with the mMessage mMachine SP6 transcription kit (Invitrogen, cat. AM1340) per the manufacturer’s protocol to produce the full-length positive-sense mRNA which was transfected into Vero cells using Lipofectamine 3000 following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 12.5 μL AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific), 0.75 μL of each primer (forward and reverse ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted from the tissue samples using TRIzol solution (Invitrogen, Mulgrave, VIC, Australia) under manufacturer’s protocol coupled with an overnight precipitation in isopropanol at −21°C to increase yields ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in fresh medium and then sonicated using a Fisherbrand Model 505 Sonic Dismembrator (Fisher Scientific, PA, USA) on ice for 45 min with alternating 10 s at 30% intensity ...
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bioRxiv - Microbiology 2024Quote: ... The synthesized TTG fragment was BP cloned into suicide vector pR6KT2G using Gateway BP Clonase II enzyme mix (Thermo Fisher Scientific) (Stice et al. ...
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bioRxiv - Microbiology 2024Quote: ... and mock community were normalized using SequalPrep Normalization Plate Kit (Invitrogen, Carlsbad, CA, USA) and pooled into equal volumes ...
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bioRxiv - Microbiology 2024Quote: The amplicon was integrated into SmaI-digested pMQ72 using the GeneArt Gibson Assembly HiFi Master Mix (Invitrogen) according to the manufacturer specifications ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4 °C followed by incubation with appropriate Alexa fluorogenic secondary antibodies (Thermo Fisher Scientific) at room temperature for 1 hours ...
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bioRxiv - Cell Biology 2024Quote: ... and GFP (Thermo Fisher Scientific) overnight at 4 °C followed by incubation with appropriate Alexa fluorogenic secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs from all cells with or without GMT/chemicals induction were extracted using Trizol Reagent (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using StepOne Real-Time PCR System (Thermo Fisher Scientific). Primer oligonucleotides were synthesized by Sigma and are listed in Supplementary Table S2.
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bioRxiv - Cell Biology 2024Quote: Calcium transient was measured with Rhod-3 Calcium Imaging Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were blocked with 4% horse serum in PBS for 1 hour and then incubated with primary antibodies against cTnT (Thermo Fisher Scientific), α-actinin (Abcam) ...
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bioRxiv - Cell Biology 2024Quote: ... RNA concentrations were measured with Nanodrop spectrophotometer (Thermo Fisher Scientific). RNA was reverse transcribed by using iScript cDNA Synthesis Kit (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: All eukaryotic expression plasmids for pUL71 and pUL71 homologues were generated in the backbone of pEF1/Myc-His C expression vector (Invitrogen) by cloning using restriction enzymes ...
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bioRxiv - Cell Biology 2024Quote: ... Quantification of libraries was performed by use of the Qubit® dsDNA HS Assay Kit (#Q32854, ThermoFisher Scientific, US).
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bioRxiv - Cell Biology 2024Quote: ... in Opti-MEM (Thermo Fisher, 51985034) or the PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... and goat anti rabbit-Alexa Fluor 488 (Thermo Fisher, A11034).
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bioRxiv - Cell Biology 2024Quote: ... the quality and quantity of the immunoprecipitated DNAs were assessed using the Qubit®Fluorometer (Thermo Fisher, Q32866) and agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent secondary antibodies: goat anti mouse-Alexa Fluor 568 (Thermo Fisher, A11031) and goat anti rabbit-Alexa Fluor 488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were used for immunoprecipitation with Protein G Dynabeads (Thermo Fisher, 10004D) associated with the 0.35 μg anti-HA rabbit mAb (CST ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cDNA was then used for real-time qPCR with the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02) using the Quant Studio™ 6 Flex Real-Time PCR system (Thermo Fisher). The mRNA levels of GAPDH or actin were used as an internal control to normalize the mRNA levels of genes of interest ...
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bioRxiv - Microbiology 2024Quote: ... and 1× SYBR green master mix (Applied Biosystems, A25742). The gyrA gene was used as an internal control.
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bioRxiv - Microbiology 2024Quote: Extracted DNA was quantified by a Qubit assay with the high sensitivity kit (Life Technologies, Carlsbad, CA, USA) and 0.2 µg/µL of DNA was sequenced by Illumina MiSeq runs (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicon was visualized in 1.5% agarose gel stained with SYBR Safe DNA gel stain (Thermo Fisher Scientific). The PCR product of the selected deletion mutant was purified using Monarch PCR and DNA cleanup kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... 20% PE: 5% PS) were prepared as described in (49) with the addition of DiOC18(3) (3,3’-Dioctadecyloxacarbocyanine Perchlorate) (Invitrogen, D275) for fluorescence ...
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bioRxiv - Microbiology 2024Quote: The amplicons were integrated into SmaI (New England BioLabs)-digested vector using the GeneArt Gibson Assembly HiFi Master Mix (Invitrogen) according to the manufacturer specifications ...
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 (Thermo scientific, Cat# MA1-140 ...
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 (Thermo scientific, Cat# 31460, RRID: AB_228341), goat anti-mouse IgG1 cross-adsorbed secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to a PVDF membrane in 1X Bolt transfer buffer (Thermo scientific, Cat# BT0006) for overnight at 25V ...
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bioRxiv - Cell Biology 2024Quote: ... Blots were then developed using an ECL reagent (Thermo scientific, Cat# 34580) in a box western blot imager (G ...
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bioRxiv - Cell Biology 2024Quote: ... 1:2000 (Thermo scientific, Cat# A10551, RRID: AB_2534048). For the IP3R1 knock down experiment ...
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bioRxiv - Cell Biology 2024Quote: ... for separation in 1X Bolt MES SDS running buffer (Thermo scientific, Cat# B0002) for 2 h at 120 V ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR was run with PowerUp SYBR green Master Mix (Thermo scientific, Cat# A25742) on a Quant Studio 3 detection system (Applied Biosystems ...