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Citations for Thermo Fisher :
9501 - 9550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed 2X in PBS and stained with 10 µg/mL Wheat-Germ Agglutinin (WGA) AlexaFluor 647 conjugate (Invitrogen, W32466) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular Ki67 staining was performed using the FoxP3 transcription factor staining kit per manufacturer protocol (ThermoFisher, 00-5523-00). Cells were fixed in 2% paraformaldehyde for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30 µg of protein was loaded onto precast gel (NP0321, NuPAGE™ 4- 12%, Bis-Tris, Mini Protein Gels, ThermoFisher Scientific, USA) and resolved at 135 volts for 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Cultured 5637 human bladder cancer cells were lysed in Pierce IP Lysis Buffer (ThermoFisher, 87787), and incubated for 30mins at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The qRT-PCR reaction was run in QuantStudio™3 Real-Time PCR System (Applied Biosystems™, USA) using the following settings ...
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bioRxiv - Cell Biology 2024Quote: ... ATF6 (Thermo Fisher, PA5-20216), XBP1 (CST ...
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bioRxiv - Cell Biology 2024Quote: Bladders of young WT and Ifrd1−/− mice were collected and homogenized in TRIzol™ Reagent (15596026, Thermo Fisher Scientific, USA) to extract total RNA followed by DNase 1 treatment (18068-015 ...
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bioRxiv - Cell Biology 2024Quote: ... p-PERK (Thermo Fisher, PA5-102853), eIF2α (CST ...
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bioRxiv - Cell Biology 2024Quote: ... to extract total RNA followed by DNase 1 treatment (18068-015, Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein was quantitated using a BCA assay (Pierce™ BCA Protein Assay Kit, 23225, Thermo Fisher Scientific, USA). 10-30 µg of protein was loaded onto precast gel (NP0321 ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was utilized to perform cDNA synthesis using SuperScript™ II Reverse Transcriptase (18064-014, Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips were mounted onto slides in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific), let dry over two nights and imaged in a Leica SP8 FALCON cLSM as described.
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bioRxiv - Microbiology 2024Quote: ... and the Ion Xpress DNA bar coding kit (Life Technologies) according to the 200-base-read Ion Proton libraries instructions of the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... Media was replaced with mock or 2.5 µM DFT supplemented FluoroBrite (Thermo Fisher Scientific) at 24 hpi ...
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bioRxiv - Microbiology 2024Quote: ... medium was removed and samples were clicked with AO-6MT in FluoroBrite (Thermo Fisher Scientific) containing Hoechst 33342 as indicated ...
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bioRxiv - Microbiology 2024Quote: ... DNA was quantified using a NanoDrop spectrophotometer (Thermo Scientific) and diluted to 5 ng/µL ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with non-essential amino acids (Thermo Fisher Scientific) and 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2024Quote: ... and stained with secondary antibody goat anti-mouse AlexaFuor594 (Thermo Fisher Scientific), unless specified ...
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bioRxiv - Microbiology 2024Quote: ... BMDM were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) GlutaMAX (Gibco #10569-010) supplemented with 1 mM sodium pyruvate ...
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bioRxiv - Microbiology 2024Quote: ... Bar-coded libraries were prepared using the Ion Plus fragment library kit (Life Technologies) and the Ion Xpress DNA bar coding kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with secondary antibody (goat anti-rabbit AlexaFluor488, Thermo Fisher Scientific) diluted in blocking buffer containing 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2024Quote: Membrane potential was measured using the potentiometric fluorescent probe DiSC3(5) (Invitrogen). Mid-log phase cells were diluted to an OD600 of 0.4 ...
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bioRxiv - Microbiology 2024Quote: ... and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX). Fragments from ∼190-250bp in length were excised ...
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bioRxiv - Microbiology 2024Quote: ... The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher). Images were captured using an inverted fluorescence microscope (Olympus-IX73).
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bioRxiv - Microbiology 2024Quote: ... The fluorochrome-labeled antibodies used were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... AlexaFluor 594 anti-human IgG (Thermo Fisher). The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... coli strain BL21(DE3) pLysS (Invitrogen) using lysogeny broth (LB ...
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bioRxiv - Microbiology 2024Quote: ... and 1% Tryptose phosphate browth (Trp; Life Technologies).
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Amplified fragments were analyzed by capillary electrophoresis using a 3730 XL DNA Analyzer (Applied Biosystems). Chromatograms were analyzed using the Osiris software (ncbi.nlm.nih.gov/osiris) ...
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bioRxiv - Microbiology 2024Quote: ... followed by sequencing using the S5 Ion torrent technology (Thermo Fisher), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 100ng of equimolar mixture of the produced fragments were transfected using the Lipofectamine 3000 kit (Invitrogen) following the supplier’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... The extracted DNA was electrophoresed on a 1% agarose gel and checked with a NanoDrop 2000 UV-vis spectrophotometer (Thermo Scientific, Wilmington, USA). PCR reactions were performed as described in a previous paper (Shen et al. ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were built adding to fragmented DNA barcode for sample identification and primers with Ion Plus Fragment Library Kit using AB Library Builder System (Thermo Fisher). To pool equimolarly the barcoded samples ...
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bioRxiv - Microbiology 2024Quote: ... Next steps included an emulsion PCR of the pools and loading on 520 chips performed using the automated Ion Chef instrument (Thermo Fisher), followed by sequencing using the S5 Ion torrent technology (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (Invitrogen, ref A10262, dilution 1:200), anti-OCT4 (StemAB ...
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bioRxiv - Cell Biology 2024Quote: ... and Ham’s F10 (RDH medium; Thermo Fisher Scientific) at 38°C in 5% CO2 until cell microinjection ...
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bioRxiv - Cell Biology 2024Quote: ... 106 B19_GFP_DPPA2mKO2 cells were co-transfected using Lipofectamine 2000 (Invitrogen) with 1 μg of pEOStagBFPplasmid plus 2 μg of the PBase-expressing vector pCAGPBase (Wang et al ...
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bioRxiv - Cell Biology 2024Quote: ... 106 cells were co-transfected using Lipofectamine 2000 (Invitrogen) with 1 μg of PB plasmid plus 2 μg of the PBase-expressing vector pCAGPBase (Wang et al ...
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bioRxiv - Cell Biology 2024Quote: ... 106 B19_GFP cells were co-transfected using Lipofectamine 2000 (Invitrogen) with 1 μg of pCAG-Cas9D10A-sgDPPA2(x2 ...
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bioRxiv - Microbiology 2024Quote: ... Individual reporter plasmids were amplified by EquiPhi29 polymerase (Thermo Fisher Scientific) and transformed into recipient B ...
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bioRxiv - Microbiology 2024Quote: ... DNA concentration was quantified with a Quant-iT dsDNA HS kit (ThermoFisher Scientific) assay in an Eppendorf AF2200 plate reader (Eppendorf UK Ltd ...
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bioRxiv - Microbiology 2024Quote: ... Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed using 4–20% gels (Invitrogen, Carlsbad, CA, USA) at 100 V for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... An AF555 conjugated mouse antibody was used to stain ZO-1 (1:100, Thermofisher Scientific) followed by incubation with a secondary-antibody to amplify the fluorescent signal ...
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bioRxiv - Microbiology 2024Quote: ... Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal, Cat# PA1-20988, Invitrogen, 1:5,000) and for virus lysate was detected with anti-p24 (mouse monoclonal ...
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bioRxiv - Microbiology 2024Quote: ... the concentration and integrity (RIN) of isolated RNA were determined using the Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher) and an RNA Standard Sensitivity Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... and the total RNA was extracted with the SPLIT RNA extraction kit (Lexogen) followed by DNase treatment with the TURBO DNase kit (Thermo Fisher). 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad) ...
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bioRxiv - Microbiology 2024Quote: ... and measured using HPLC (Dionex Ultimate 3000, Thermo Fisher Scientific, USA) equipped with an ion exclusion column (Aminex® HPX-87X ...
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bioRxiv - Microbiology 2024Quote: ... RNA was quantified using qubit fluorometer (Thermofisher) and integrity was assessed using fragment analyzer (Agilent Technologies) ...