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Citations for Thermo Fisher :
9351 - 9400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Reactions were carried out on a QuantStudio3 (Applied Biosystems) real-time PCR machine using the following program ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated in 50 nM MitoTracker Red CMXRos (Invitrogen) for 30 minutes prior to agarose embedding ...
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bioRxiv - Developmental Biology 2024Quote: ... The slides were further incubated with Alexa Fluor 488-conjugated anti-mouse IgG and Alexa Fluor 568-conjugated anti-rabbit IgG (Molecular Probes, Eugene, OR) for 1 hour at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Capped messenger RNA was synthesized using the mMESSAGE mMACHINE kit (Ambion), and 500 pg of each mRNA was injected at the one- or two-cell stage.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were grown with DMEM/F12 containing 10% FBS on 8-well Lab-Tek chamber slides (Nunc Lab-Tek Chamber Slide System, Thermo Scientific) precoated with 0.1% gelatin in PBS overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... Wnt11 and mini-LRP1 capped RNA for injections was prepared using T7 and SP6 mMessage mMachine kit (ThermoFisher, AM1344). Translation blocking morpholino antisense oligonucleotides targeting lrp1 (5’-TGCCTTGATCCAGTTCTTGG-3’ ...
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bioRxiv - Developmental Biology 2024Quote: ... or Hoechst (Life Technologies corporation) in PBST for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA concentration and purity were assessed with the Nanodrop ND-1000 spectrophotometer (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: ... ttp and g3bp was assessed with GeneArt Genomic Cleavage Detection Kit (Thermofisher), starting from genomic DNA extracted from initial tailbud embryos co-electroporated with specific sgRNAs and Eef1a>Cas9 before the first cell division ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated in Alexa Fluor 488 goat anti-mouse IgG (H+L) (Invitrogen), 1:500 in blocking solution for 1 h ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were treated with 100 nM Rhodamine Phalloidin (Thermo Fisher Scientific) or Acti-stain 488 fluorescent Phalloidin (Cytoskeleton ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 1 µg/ml DAPI (Thermo Fisher Scientific) or Hoechst (Life Technologies corporation ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.001 M sodium pyruvate (Thermo Fisher Scientific, Cat# BP356), and 0.1 mg/ml DNase (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... and PE anti-mouse CD207 (Invitrogen, Cat# 12-2075-82, Clone eBioL31).
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bioRxiv - Developmental Biology 2024Quote: ... mounted in Prolong Gold without DAPI (Invitrogen) and put in the dark to cure overnight before imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... and goat anti-rabbit Alexa 568 (Invitrogen, all diluted 1:2000 in PBSTB) for 2 h at room temperature in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... and anti-guinea pig HRP-conjugated secondary antibodies (ThermoFisher) were diluted 1:10,000 in 1x TBST containing 5% milk for 1h at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were washed 3X in PBSTB for 10 min and incubated with secondary antibodies: anti-mouse Alexa 488 (Molecular Probes), anti-guinea pig 633 ...
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bioRxiv - Molecular Biology 2024Quote: Nanoflow-LC-MS/MS for sample analysis was performed with an Orbitrap Fusion Lumos mass spectrometer coupled to an Easy nano-LC 1200 HPLC (Thermo Fisher Scientific). For peptide separation by HPLC a gradient of increasing organic proportion (buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... Gateway cloning was performed according to the manufacturer’s protocol (Thermo Fisher Scientific). All other TAFs were cloned into expression vectors in the same manner ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were washed three times in NLB and bound proteins were eluted in 30 uL 1x NuPAGE LDS (Thermo Fisher Scientific, NP0007), supplemented with reducing agent ...
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bioRxiv - Molecular Biology 2024Quote: ... 2μg of RNA was reverse-transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems) per manufacturer’s protocol ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Cells were labelled with 1:300 dilution of anti-human HMGCR (ThermoFisher, PA537367) or HMGCS1 (ThermoFisher ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... the sections were incubated with Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 546 donkey anti-rabbit IgG (both 1:200; Invitrogen, Thermo Fisher Scientific) as secondary antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... ESCs were first transduced with lentiviral packaged pLX_311 and selected with Blasticidin (Gibco; A1113903) to generate stable dCas9-KRAB expressing cell lines ...
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bioRxiv - Developmental Biology 2024Quote: Attached cells or embryoid bodies in suspension were dissociated using Trypsin-EDTA (Gibco; 25200072) for 3 minutes at room temperature before adding serum-containing media ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... sections were incubated with Alexa Fluor 647 chicken anti-mouse IgG (1:200, Invitrogen, Thermo Fisher Scientific) as a secondary antibody (Figure 5C,D).
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bioRxiv - Molecular Biology 2024Quote: ... co-stained with DAPI (Thermo Fisher Scientific Waltham, MA). Immunofluorescence detection of alpha-smooth muscle actin (αSMA ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... sections were incubated with Alexa Fluor 647 chicken anti-mouse IgG (1:200, Invitrogen, Thermo Fisher Scientific) as a secondary antibody (Figure 5C,D).
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bioRxiv - Molecular Biology 2024Quote: ... 0.5X non-essential amino acids (GIBCO 11140), 100 U/mL Penicillin-Streptomycin (GIBCO 15140) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1x GlutaMAX (Thermo Fisher Scientific), 1x ESC-qualified nucleosides (EMD Millipore) ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies included Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 546 donkey anti-rabbit IgG (both 1:200; Invitrogen, Thermo Fisher Scientific). Slides were mounted using DAPI (Molecular Probes ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies included Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 546 donkey anti-rabbit IgG (both 1:200; Invitrogen, Thermo Fisher Scientific). Slides were mounted using DAPI (Molecular Probes ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by Qubit dsDNA quantification (Invitrogen Q32853), and 2100 BioAnalyzer D1000 kit (Agilent 50671504 ...
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bioRxiv - Developmental Biology 2024Quote: ... 1-2 drops of ProLong Gold Antifade Mountant (ThermoFisher Scientific, Cat# P36930) were placed on the slides and mounted with a coverslip ...
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bioRxiv - Developmental Biology 2024Quote: ... dsRNA was synthesised from these templates using the MEGAscript T7 transcription kit (Invitrogen) and precipitated using LiCl ...
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bioRxiv - Developmental Biology 2024Quote: ... N2 (Life Technologies), 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: Purified mouse BAC DNAs Sptlc2 (RP23-460P20 / 12D2 / chr12:87239078-87411743 GRCm39) and Sptlc2 (RP23-285E19 / 12D2 / chr12:87395112-87476186 GRCm39) were labeled with a red-dUTP (AF594, Molecular Probes) by nick translation ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 μL of Fermentas FD Eco31I (Thermo Fisher Scientific, FD0293), and 0.1 μL of 20 mg/mL BSA (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was then isolated by running on E-Gel EX 1% Agarose Gels (ThermoFisher G401001), excising the band at the expected length ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of RNA of each sample was mixed with 2 µl of ERCC ExFold spike-in RNA mixes based on the RNA ratio recommended by the protocol (Invitrogen 4456739), rRNA were depleted by hybridization of complementary DNA oligonucleotides and treatment with RNase and DNase ...
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bioRxiv - Molecular Biology 2024Quote: ... N2a cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco, Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... β-mercaptoethanol 0.1 % (Thermo Fisher #31350010). Cells were seeded in a specific density of 1.5 x 105 cells/ml in ultra-low attachment dishes (Corning #3261 ...
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bioRxiv - Developmental Biology 2024Quote: ... and Neurobasal Medium (Gibco)] supplemented with N2 (Thermo Fisher #17502048), B27 without Vitamin A (Thermo Fisher #12587010) ...
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bioRxiv - Developmental Biology 2024Quote: ... penicillin/streptomycin 1 % (Thermo Fisher #15140122), β-mercaptoethanol 0.1 % (Thermo Fisher #31350010) ...
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bioRxiv - Developmental Biology 2024Quote: ... and Neurobasal Medium (Gibco)] supplemented with N2 (Thermo Fisher #17502048) ...
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bioRxiv - Developmental Biology 2024Quote: ... B27 without Vitamin A (Thermo Fisher #12587010), penicillin/streptomycin 1 % (Thermo Fisher #15140122) ...
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bioRxiv - Developmental Biology 2024Quote: ... Advanced DMEM/F-12 (Gibco) and Neurobasal Medium (Gibco)] supplemented with N2 (Thermo Fisher #17502048) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cortical neurons were plated at a density of ∼500 cells/mm2 (for RNA or protein isolation) or ∼250 cells/mm2 (for immunocytochemistry) on tissue culture plates or coverslips (Fisher Scientific, NC0672873) coated with 0.1mg/mL poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: We maintained mouse embryonic stem cells (E14) in ESC Media containing: DMEM (Fisher Scientific) supplemented with 15 % ESC-qualified fetal bovine serum (Thermo Fisher Scientific) ...