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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and Opti-MEM reduced serum medium (Thermo Fisher Scientific, #31985062) in a 12-well plate following the manufacturer’s instruction (Yusa et al ...
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bioRxiv - Cell Biology 2024Quote: ... Null cells were selected for with supplementation of 10 µg/mL Blasticidin S (Thermofisher). Transformed cells (see Generation of Transgenic Lines) ...
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bioRxiv - Cell Biology 2024Quote: ... and/or 50 µg/mL Hygromycin B (Thermofisher).
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bioRxiv - Cell Biology 2024Quote: ... as measured via Countess II FL (Thermofisher), and a minimum of 10 mLs were harvested ...
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bioRxiv - Cell Biology 2024Quote: ... were co-transfected with pMK467 (dTAG-BromoTag-mAID-EGFP-NLS) and pCS-TP (TOL2 transposase) with the Neon system (Thermo Fisher Scientific) (Sumiyama et al ...
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bioRxiv - Cell Biology 2024Quote: ... 2 (ThermoFisher Scientific; 4390846), CCDC51 oligo #1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and DNM1L/Drp1 (Thermo Fisher Scientific; s19560). CCDC51 oligo #1 was used in all experiments unless stated otherwise ...
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bioRxiv - Cell Biology 2024Quote: ... CCDC51 oligo #1 (Thermo Fisher Scientific; s36162), CCDC51 oligo #2 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were carried out with Lipofectamine 3000 (ThermoFisher Scientific) according to manufacturer’s instructions for 5-6 hours ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 25nM MitoTracker CMXRos (Thermo Fisher Scientific; M7512) in DMEM for 30-60 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... CCDC51 oligo #2 (Thermo Fisher Scientific; s36164), and DNM1L/Drp1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... transfections were performed in 6-well plates with Lipofectamine RNAiMAX (ThermoFisher Scientific) and 20 nM RNAi oligonucleotides according to manufactuer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% penicillin-streptomycin (Invitrogen)) was used for cell culture ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse anti-FAK (ThermoFisher Scientific; 34Q36), Rabbit anti-phospho-FAK (Y925 ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were analyzed by reversed-phase nLC-MS/MS using an Ultimate 3000 UHPLC coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18 ...
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bioRxiv - Cell Biology 2024Quote: ... MS/MS fragment spectra were searched using Sequest HT in Proteome Discoverer (Thermo Scientific) against a human database (UniProt ...
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bioRxiv - Molecular Biology 2024Quote: ... Reporter stable cell line was generated by transfection (Lipofectamine™ 2000, thermofisher) and drug selection (Hygromycin, Thermofisher).
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bioRxiv - Molecular Biology 2024Quote: ... and 50 μM β-mercaptoethanol (Gibco, 21985023). To generate Ogt iKO mESC carrying the 2C-reporter we used the MERVL::tdTomato reporter [51] that was kindly shared from Samuel Pfaff (Addgene #40281) ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibiotic selection was performed by culturing the transfected mESC with 1 um puromycin (Thermo Fisher, A1113803) for 2 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... sfGFP::CENP-A were acquired from the Needleman Lab (Harvard University) and cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... All libraries were assessed using Qubit™ RNA HS Assay Kit (ThermoFisher, Q32855) and TapeStation High Sensitivity RNA ScreenTape Analysis (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... AHR- or OVOL1-specific siRNA (generated by Huzhou Hippo Biotechnology Co., Ltd) was transfected into NHEKs using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific, Cat# 13778075) per manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% FBS (Thermo Fisher Scientific) and Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... coated Protein-G magnetic beads (Pierce™ ThermoFisher Scientific) were used to capture BG4-G4 complexes ...
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bioRxiv - Molecular Biology 2024Quote: ... lysates were prepared in NuPAGE LDS sample buffer (Thermo Fisher Scientific, NP0007) and reducing agent ...
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bioRxiv - Molecular Biology 2024Quote: ... epidermal cells from mouse back skin were incubated with H2DCFDA (Invitrogen, Cat# D399) at a concentration of 10 μM at 37℃ for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 80 °C for 10 min and proteins separated using precast NuPAGE 4–12 % Bis-Tris gels (Thermo Fisher Scientific, NP0322PK2) at 80 V for ca ...
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bioRxiv - Cell Biology 2024Quote: ... was employed to extract the RNA according to the manufacturer’s instructions and then quantified using a NanoDrop spectrophotometer (ThermoFisher Scientific). Quantinova Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μM of DHE was added in HBBS medium (Hanks’ Balanced Salt Solution, Thermo fisher, 24020117) and the increase in intensity measured continuously over time using a UV Nikon microscope.
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bioRxiv - Cell Biology 2024Quote: ... Transfection was performed using Lipofectamine 3000 transfection reagent (Thermo Fisher, L3000015) according to the manufacturer’s instructions and following optimization.
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bioRxiv - Cell Biology 2024Quote: ... and 1% of 100 U/mL Penicillin and 100 mg/mL streptomycin (Thermo Fisher, 15140122). GE-180 was a gist by General Electric Healthcare ...
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bioRxiv - Cell Biology 2024Quote: The Amplex Red Cholesterol Assay Kit (Thermo Fisher, A12216 was used to quantify cholesterol levels ...
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bioRxiv - Cell Biology 2024Quote: ... has been added to 50 μl of magnetic beads linked to Protein A (ThermoFisher Scientific, 10001D) for 15 minutes at RT to induce the binding ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies were conjugated to Alexa Fluor dyes (Life Technologies, UK). Following washing (3 x 15 minutes using PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... L-Glutamine (4 mM) and Sodium Pyruvate (1 mM) (Thermo Fisher, 11995065), supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% Fetal Bovine Serum (FBS) (Thermo Fisher, 10082147) and 1% of 100 U/mL Penicillin and 100 mg/mL streptomycin (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 10 U of RNAse A (Thermofisher, Cat# 19101) was diluted into 1 ml total of buffer 3 ...
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bioRxiv - Cell Biology 2024Quote: ... Lysate concentrations were measured at 488nm absorbance using NanoDrop ND-1000 spectrophotometer (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: IMCD-3 cells expressing mScarlet tubulin were then transfected in OptiMEM media (Thermo Scientific) using Lipofectamine 2000 (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.01 M HEPES (Thermo Fisher Scientific, Cat# BP310), 0.001 M sodium pyruvate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were desalted through a C18 matrix (HyperSep SpinTip C-18, Thermo Fisher Scientific). 0.1% Trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2024Quote: ... skin was minced using a fine scissor and then digested with 10 ml of RPMI 1640 Medium (Gibco, Cat# 11875093) containing 0.25% collagenase (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA of human TAF2 was amplified by PCR with gene-specific primers fused to attB recombination sequences for Gateway® cloning (Thermo Fisher Scientific). The amplified sequence was recombined via BP reaction into the pDONR201 donor resulting in pENTR-TAF2 for N-terminal tagging ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Trifluoroacetic acid (TFA; Thermo Fisher Scientific) in ddH2O was used as binding and washing solution and the tryptic peptides were then eluted from the tips with a releasing solution of 65% acetonitrile (LC-MS grade ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells with surface staining only were incubated with SYTOX™ Blue Dead Cell Stain (Invitrogen, Cat# S34857) for 5 min before analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... FITC anti-mouse MHC II (Invitrogen, Cat# 11-5321-82, Clone M5/114.15.2), and PE anti-mouse CD207 (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... utilizing Lipofectamine 2000 (Thermo Fisher Scientific). Following a 24-hour incubation period ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor-conjugated secondary antibodies for immunofluorescence staining were purchased from Invitrogen.
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bioRxiv - Developmental Biology 2024Quote: ... the transfected cells underwent lysis in 800 µl of RIPA buffer (Thermo Fisher Scientific) supplemented with complete ...