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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was pre-cleared with 30 μg of Dynabeads Protein G/A (ThermoFisher) for 30 min under rotating mixing ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was harvested using 1 ml Trizol (ThermoFisher Scientific) according to the manufacturer’s protocol and resuspended in Formamide (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... before Fab was purified with CaptureSelect™ IgG-CH1 Affinity Matrix (ThermoFisher). Bound Fab was eluted in a buffer containing 100 mM glycine pH 3.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... or control rabbit IgG (ThermoFisher), goat anti-DDX5 (ab10261 ...
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bioRxiv - Molecular Biology 2024Quote: ... duplicate wells were treated with Hoechst 3342 (ThermoFisher Scientific) for 45min ...
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bioRxiv - Molecular Biology 2024Quote: ... or PowerUp™ SYBR™ Green Master Mix (ThermoFisher). Forty reaction cycles of 15 s at 95 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phalloidin 647 Plus (Invitrogen, A30107, 1:400) to visualize nuclear and F-actin ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3X with DPBS and stained with small molecule DNA stain Hoechst 33342 (Invitrogen, H3570, 1:1000, 10µg/mL), and Phalloidin 647 Plus (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... permeabilized with 0.5% Triton X-100 in DPBS for 20 minutes and stained for 30 minutes each with Click-iT reaction cocktail containing Alexa Fluor 488 Azide and 5ug/ml Hoechst 33342 (Invitrogen, H3570). The plate was imaged at 10x (MRF00101 ...
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bioRxiv - Molecular Biology 2024Quote: ... an initial cell count cell was conducted using cell-permeable DNA stain Hoechst 33342 (Invitrogen, H3570, 1:1000, 10µg/mL). Whole wells were stained and imaged in triplicate every day for three days using a 2X objective (Nikon ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated from 1 mg of total RNA using random hexamers and Maxima Reverse Transcriptase (Thermo Fisher Scientific). Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and Lyso-Tracker Red are from Invitrogen. Anti-caspase-3 (#CST-9662) ...
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bioRxiv - Cancer Biology 2024Quote: ... 75 µM ID column packed with C18 beads (1.7 µm) (IonOpticks) using a Vanquish Neo (Thermo Fisher Scientific) UHPLC ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM GlutaMAX (Thermo Fisher Scientific), 5 ng/ml human insulin (Sanofi ...
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bioRxiv - Cancer Biology 2024Quote: ... HepaRG cells were cultured in WEM (Thermo Fisher Scientific) with 10% FCS ...
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bioRxiv - Cancer Biology 2024Quote: ... Dulbecco’s Modified and Eagle Medium (DMEM) were procured from Gibco, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ...
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bioRxiv - Cancer Biology 2024Quote: ... SW480 and SW620 were cultured in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) with 7% fetal calf serum (FCS) ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantified via Nanodrop spectrophotometer (Thermo scientific). The sequencing library was generated by combining equal amounts of purified PCR products and quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... The library was amplified by large-scale transformation of Stbl3 cells (ThermoFisher Scientific) and grown on LB plates containing ampicillin ...
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bioRxiv - Molecular Biology 2024Quote: ... and counterstained with Alexa-Fluor conjugated secondary antibodies (Invitrogen A11001 & A11011, 1:500, 4µg/mL) for 2 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Hoechst 33342 (Invitrogen, H3570, 1:1000, 10µg/mL), and Phalloidin 647 Plus (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies) using the Luna qPCR Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse HA-probe antibody (Thermofisher, 2-2.2.14, 1:2000), rabbit HA-probe antibody (CST ...
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bioRxiv - Molecular Biology 2024Quote: ... or developed with SuperSignal West Femto kit (ThermoFisher) and scanned with KwikQuant Imager (KindleBiosciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... at which point fibroblasts were treated for 24 h with 10µM EdU solution (Invitrogen, C10337). 24 h after EdU treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... Bolt 8% Bis-Tris Plus gels (Invitrogen) or self-cast 10% acrylamide gels (0.375 M Tris pH 8.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Hoechst 33342 (Invitrogen, H3570, 1:1000, 10µg/mL), and Phalloidin 647 Plus (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were resolved on precast NuPAGE Novex 4–12% Bis-Tris gels (Invitrogen), Bolt 8% Bis-Tris Plus gels (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was measured using the Alexa Fluor™ 488 Annexin V conjugate with Propidium Iodide (ThermoFisher Scientific) according to the manufacturer’s instructions and analysed by flow cytometry (BD LSRFortessa™ X-20 ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phalloidin 647 Plus (Invitrogen, A30107, 1:400). Plates were imaged at 10x (MRF00101 ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were TMT labelled using the TMT 10-plex isobaric label reagents (Thermofisher) and fractionated on a Waters XBridge BEH C18 (3.5 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phalloidin 647 Plus (Invitrogen, A30107, 1:400). Plates were imaged at 10x (MRF00101 ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunoprecipitated complexes were collected using Protein G Dynabeads (Invitrogen) and washed with 1000 μL of respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractionated samples and bead-enriched samples were individually loaded onto a C18 trap column (3 μm, 75μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phalloidin 647 Plus (Invitrogen, A30107, 1:400). Plates were imaged at 10x (MRF00101 ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were ionized using a nanospray ion source into a Q-Exactive HF mass spectrometer (Thermo Fisher Scientific). MS/MS spectra were searched against the Uniprot human complete proteome FASTA database downloaded on 2018_10_26 ...
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bioRxiv - Molecular Biology 2024Quote: ... Hoechst 33342 (Invitrogen, H3570, 1:1000, 10µg/mL), and Phalloidin 647 Plus (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG M2 or HA-tag antibody at 4 °C overnight before precipitated with magnetic Protein G beads (Thermofisher) for 2 hours at 4 °C followed by 3 washes with RIPA buffer.
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bioRxiv - Cancer Biology 2024Quote: ... V5-tag (46-0705, Invitrogen)(1.12 ug antibody/500 ug protein IP ...
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bioRxiv - Cancer Biology 2024Quote: Transfections of siRNAs were performed using Lipofectamine 3000 (L3000015, Thermo Fisher Scientific). Cells were reverse transfected with final siRNA concentration of 12.5 nM siRNA ...
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bioRxiv - Cancer Biology 2024Quote: Cells were washed with PBS and harvested in TRIzolTM reagent containing phenol (15596018, Life Technologies). For total RNA extraction ...
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bioRxiv - Cancer Biology 2024Quote: crRNA:tracrRNA duplexes were transfected into RPE Cas9 cells by reverse transfection using Lipofectamine 3000 transfection reagent (L3000015, Thermo Fisher Scientific) with a final crRNA:tracrRNA concentration of 25 nM (12.5 nM upstream crRNA:tracrRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and the upper aqueous phase was used for precipitating the RNA by adding 500 µL isopropanol – and optionally 2 µL GlycoBlue Coprecipitant (AM9516, Thermo Fisher Scientific) – and centrifuging ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS) and 2 µL 20 mg/mL proteinase K (25530049, Thermo Fisher Scientific). Samples were incubated overnight at 65 °C and 800 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... and resuspended in nuclease-free H2O (AM9939, Life Technologies). RNA concentration was measured by spectrophotometry.
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bioRxiv - Cancer Biology 2024Quote: For immunoblotting cells were harvested in RIPA lysis buffer supplemented with a protease and phosphatase inhibitor cocktails (Thermo Fisher). Lysis was allowed to proceed for 30-60 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... and chromatin fractions was carried out using a Subcellular Protein Fractionation Kit (Thermo Fisher #78840).
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated in PBS with 1.5 mM dithiobis (succinimidyl propionate) (DSP, Thermo Fisher #22585) for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell nuclei were then extracted and resuspended in 1/2 dilution IP buffer (Thermo Fisher #87788) and sonicated for 2 min (5s on/5s off ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were determined by Bicinchoninic acid (BCA) assay (Thermo Fisher). Lysates were prepared with 4X Laemmli buffer (BioRad ...