Labshake search
Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... ovarioles were stained with CellMask Orange Plasma membrane Stain (ThermoFisher; diluted 1:10 000 in culture medium) for 15 minutes and washed with ex vivo culture medium before imaging ...
-
bioRxiv - Cell Biology 2024Quote: ... Each EM grid was applied with 4 µl sample and vitrified by plugging into liquid ethane using the Vitrobot Mark IV system (Thermo Fisher Scientific). The cryo-EM samples were examined using an FEI Tecnai Arctica 200 kV transmission electron microscope ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were resolved by SDS-PAGE and transferred to a nitrocellulose membrane using the iBlot Dry Blotting System (Invitrogen), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... NRK cells and HEK 293FT cells were grown in DMEM (Gibco, C11995500BT) supplemented with 10% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... 1% (v/v) glutamax (Gibco, 35050-061) and 1% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... ESCs were electroporated with the linearized vector using XhoI and selected in 150 μg/mL G418 (Thermo Fisher Scientific). Clones were validated by genomic PCR using the following primers ...
-
bioRxiv - Cell Biology 2024Quote: Equal amounts of cells were lysed in 1X LDS sample buffer (Life Technologies) containing 10% NuPAGE reducing agent (Life Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 1% streptomycin and penicillin (GIBCO, 15140122). The cells were grown under conditions of 37°C with 5% CO2 and tested for mycoplasma regularly.
-
bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA assay (Thermo Scientific Scientific) and samples were diluted in lysis buffer (50 mM Tris pH 6.8 ...
-
bioRxiv - Molecular Biology 2024Quote: Cells expressing Corelet system constructs (NLS-iLID-GFP-Ferritin and either BRD4dN-mCh-sspB or BRD4FL-mCh-sspB) via lentiviral transduction were seeded on 96-well glass bottom plates (ThermoFisher Cat. No NC0536760), then loaded onto a Nikon A1 laser scanning confocal microscope with Nikon Eclipse Ti2 body ...
-
bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies used were Alexa Fluor 488 anti-rabbit (A-21206; Thermo Fisher Scientific; 1:200), Alexa Fluor 488 anti-mouse (A-21202 ...
-
bioRxiv - Cell Biology 2024Quote: ... males were picked onto an OP50 plate and treated with 200 uL 0.05 mM working stock of MitoTracker Deep Red FM (Invitrogen) or MitoTracker Red CMXros (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... or MitoTracker Red CMXros (Invitrogen) in M9 overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... The primary antibodies used in this work were mouse monoclonal anti-tubulin (DM1α; Thermo Fisher Scientific; 1:200) and rabbit anti-GFP (NB600-308 ...
-
bioRxiv - Cell Biology 2024Quote: ... and Alexa Fluor 594 anti-mouse (A-21203; Thermo Fisher Scientific; 1:200). Z-stacks were captured at 1-μm steps for meiotic embryos using the same microscope described above for live imaging.
-
bioRxiv - Cell Biology 2024Quote: ... and/or 0.2U/mL Alexa Fluor 647-labeled phalloidin (Invitrogen) for 35-45min as detailed in Table S2 ...
-
bioRxiv - Cell Biology 2024Quote: ... coverslips were mounted on glass slides in ProLong Gold anti-fade (Invitrogen).
-
bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 488 anti-mouse (A-21202; Thermo Fisher Scientific; 1:200) and Alexa Fluor 594 anti-mouse (A-21203 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1mL Glutamax (Gibco-#35050-061), 4.4mg Vitamin C (Sigma-#A4403) ...
-
bioRxiv - Cell Biology 2024Quote: ... 1×106 mESCs/sq.cm plated with KnockOut DMEM (Gibco-10829-018) supplemented with 2% FBS and incubated overnight at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1X Penicillin/streptomycin (Gibco-#15240-062), make up to 100mL with MCDB (Sigma-#M6770)] with 100ng/mL Activin (Sino Biological-#10429 ...
-
bioRxiv - Cell Biology 2024Quote: ... with UNG (ThermoFisher, Waltham, MA, Cat. no. 4440038), was used to perform qRT-PCR of cDNA ...
-
bioRxiv - Cell Biology 2024Quote: ... or 647 nm depending on the incorporated fluorophore using a Nanodrop™ 2000 spectrophotometer (Thermo Fisher Scientific). To assess the quality of generated probes ...
-
bioRxiv - Cell Biology 2024Quote: ... The cell suspension was diluted 10-fold with EMM2 liquid medium without thiamine in a 96-well DeepWellTM plate (ThermoFisher, #260251), and this was subjected to incubation at 33°C for 6 h with shaking at 900 rpm ...
-
bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 2000 (Thermo Fisher Scientific), as we have previously described (46,49,50) ...
-
bioRxiv - Cell Biology 2024Quote: ... washed twice with Opti-MEM (Thermo Fisher scientific) and resuspended in BTXpress solution (BTX) ...
-
bioRxiv - Cell Biology 2024Quote: ... 1× B-27 Supplement (Life Technologies), 1.25 mM N-acetylcysteine (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... and phalodin 488 1/400 (Invitrogen, A12380) for 1 h in the dark at RT and then washed 3 x 5 min ...
-
bioRxiv - Cell Biology 2024Quote: ... and 10% fetal bovine serum (Gibco 16140071) were added to enhance transfection efficiency (Yuan et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... The blocking buffer was supplemented with 0.1 mg/mL sheared salmon sperm DNA (Thermo Fisher 15632011) and 0.05% w/v dextran sulfate (Merck D4911 ...
-
bioRxiv - Cell Biology 2024Quote: ... SlowFade Diamond (Invitrogen S36967) or ProLong Gold (Invitrogen P36930).
-
bioRxiv - Cell Biology 2024Quote: ... and Image-iT FX reagent (Invitrogen I36933) was applied for 10 min after blocking to reduce nonspecific imager binding (Koester et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... or ProLong Gold (Invitrogen P36930).
-
bioRxiv - Cell Biology 2024Quote: ... and fixed for 10 min by 4% paraformaldehyde (PFA) diluted in Dulbecco’s PBS containing Ca2+ and Mg2+ (DPBS; Gibco 14040083), then blocked and permeabilized for 1 h in blocking buffer (10% bovine calf serum and 0.1% Triton X-100 in DPBS) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 200 ng of total RNA was used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit using random primers (Applied Biosystems). RT-qPCR was performed by the CFX96 Real-Time PCR system (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... while cDNAs encoding hsINTS1 and hsINTS8 were ordered as string DNAs from Thermo Fisher Scientific.
-
bioRxiv - Molecular Biology 2024Quote: ... linearized with NotI and mRNA was synthesized with mMESSAGE mMACHINE™ SP6 Transcription Kit (Invitrogen, AM1340). mRNA (400 ng/μl ...
-
bioRxiv - Molecular Biology 2024Quote: Cells from 6-well plates were trypsinized (Gibco) and collected in Eppendorf tubes ...
-
bioRxiv - Molecular Biology 2024Quote: The nuclei were stained with DAPI (Cell Signalling Technology) and the coverslips were washed and mounted using ProLong™ Gold Antifade mounting media (Invitrogen). Fluorescent images were obtained by using Zeiss Axiovert 200M fluorescence inverted microscope and analysed by CellProfiler software [33].
-
bioRxiv - Molecular Biology 2024Quote: ... supplemented with 15% Fetal Bovine Serum (FBS) (Gibco). Lipofectamine LTX with PLUS reagent (Invitrogen by Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 100 mg/mL streptomycin (Thermo Fisher Scientific). TAP cell lines were generated based on the HEK293 Flp-In T-REx system (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... in a StepOnePlus™ Real-Time PCR System (Applied Biosystems) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: Molecular biology enzymes and kits were purchased from Thermo Fisher Scientific (Madrid ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were maintained in Dulbecco’s modified Eagle’s medium: nutrient mixture F12 (DMEM/F12; Thermo Fisher Scientific) with 10% (v/v ...
-
bioRxiv - Microbiology 2024Quote: ... and 1% (v/v) penicillin‒streptomycin (Thermo Fisher Scientific). Cells were maintained in a humidified incubator at 37 °C and 5% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA) and 10% FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... were exposed to DNA-lipid complexes formed by a 1:1 mixture of Lipofectamine® 2000 Reagent (Thermo Fisher Scientific) and the aforementioned plasmid in Opti-MEM medium (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2024Quote: ... with each well containing 100 uL of Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific), supplemented with 1% penicillin-streptomycin (GIBCO ...
-
bioRxiv - Cell Biology 2024Quote: ... Human MPB CD34+ cells from a healthy volunteer were thawed using chilled Iscove’s Modified Dulbecco’s Medium (IMDM) (Thermo Fisher Scientific, Waltham, MA, USA,) supplemented with 2% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 1% penicillin-streptomycin (GIBCO), 100 ng/mL SCF ...