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Citations for Thermo Fisher :
9251 - 9300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... UHPLC system coupled online with an Orbitrap Fusion Tribrid (Thermo Scientific) mass spectrometer ...
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bioRxiv - Molecular Biology 2024Quote: Total EV-RNA was extracted with TRIzol reagent (Ambion, Life Technologies) and chloroform precipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Absorbance (570 nm) was measured at a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific) and cell viability was calculated as % with respect to untreated cells (DMEM only).
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bioRxiv - Molecular Biology 2024Quote: Tissues were fixed in 4% paraformaldehyde (PFA)/PBS solution (Affymetrix/USB) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... rabbit anti-HDAC2 (Thermo Fisher Scientific, 51-5100, 1:1000); rabbit anti-HDAC3 (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse anti-KRT14 (Invitrogen, #MA5-11599, 1:500); rat anti-Ki-67 (eBioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... A Hypersil Gold C18 column (Thermofisher, 100 x 2.1 mm, particle size: 1.9 µm) was used for separation ...
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bioRxiv - Molecular Biology 2024Quote: ... 340 μl DMEM already containing 1.25 nM LysoTracker™ Red DND-99 (L7528, Invitrogen) and 1 µg/ml Hoechst (62249 ...
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bioRxiv - Molecular Biology 2024Quote: ... was diluted 1:200 and AF488 Donkey anti-rabbit (Life Technologies; A21206) was diluted 1:200 in staining buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... and X-ray films (ThermoFisher, 34090).
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bioRxiv - Molecular Biology 2024Quote: ... and Halt™ Protease/Phosphatase Inhibitor Cocktail (ThermoFisher, 78441) on ice for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... placed on slides using Fluoromount-G Mounting Medium (Invitrogen; 00-4958-02), and cover slipped.
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bioRxiv - Molecular Biology 2024Quote: ... and Penicillin-Streptomycin (Thermo Fisher Scientific) in a humidified incubator with 5% CO2 maintained at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The dissociated mESC were first labeled with the eBioscienceTM Fixable Viability Dye eFluorTM 780 (ThermoFisher, 65-0865-14) to select viable cells and then stained for surface markers with indicated fluorochrome-conjugated antibodies diluted in FACS buffer (PBS 1X ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100µM uridine-5’-triphosphate trisodium (UTP) salt (Thermo Scientific Chemicals #AAJ63427MC) apically.
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bioRxiv - Molecular Biology 2024Quote: NHEKs were cultured in Keratinocyte Serum-Free Medium (Gibco, Cat# 17005042) per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins from WCEs were resolved using NuPAGE 4-12% bis-tris gel (Thermo Fisher Scientific, NP0321BOX) and transferred onto polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The tissues were cultured in DMEM medium (Gibco, Cat# 11965092) containing 10% FBS and treated with AGX51 (100 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA with ligated adaptors was then treated with sodium bisulfite (MethylCode, Thermo Fisher Scientific) for 4hrs and amplified with index primers using KAPA HiFi HotStart Uracil+ Ready Mix (KAPA Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: For samples prepared with the CryoCycle blotting method inside a TFS Vitrobot Mark IV chamber (Thermo Fisher Scientific) (Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted using Trizol reagent (Invitrogen, Waltham, MA). Library preparation ...
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bioRxiv - Molecular Biology 2024Quote: ... 15% KOSR (KnockOut Serum Replacement; ThermoFisher Scientific, 10828028), GlutaMAXTM (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... The fractions were analysed using the same DIA method as described above on an Orbitrap 480 spectrometer (Thermo Scientific), coupled to an Evosep One liquid chromatography system ...
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bioRxiv - Molecular Biology 2024Quote: Samples were screened on a TFS Glacios cryoTEM (Thermo Fisher Scientific) and a TFS Falcon 3EC camera operating in integrating mode using either Smart Leginon Autoscreen (Cheng et al. ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was performed using an ABI 7500 real-time PCR system and SYBR Green dye (Applied Biosystems, Foster City, CA, USA). The mRNA expression levels were normalized to the endogenous GAPDH housekeeping gene ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
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bioRxiv - Cell Biology 2024Quote: ... in PBS-/- (Life Technologies, #14190250). Methods on the cell line generation and characterization can be found in the Supplementary materials.
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bioRxiv - Cell Biology 2024Quote: ... were selected for with additional supplementation of 20 µg/mL G418 Sulfate (Thermofisher) and/or 50 µg/mL Hygromycin B (Thermofisher).
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bioRxiv - Cell Biology 2024Quote: ... and Opti-MEM reduced serum medium (Thermo Fisher Scientific, #31985062) in a 12-well plate following the manufacturer’s instruction (Yusa et al ...
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bioRxiv - Cell Biology 2024Quote: ... Null cells were selected for with supplementation of 10 µg/mL Blasticidin S (Thermofisher). Transformed cells (see Generation of Transgenic Lines) ...
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bioRxiv - Cell Biology 2024Quote: ... and/or 50 µg/mL Hygromycin B (Thermofisher).
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bioRxiv - Cell Biology 2024Quote: ... as measured via Countess II FL (Thermofisher), and a minimum of 10 mLs were harvested ...
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bioRxiv - Cell Biology 2024Quote: ... were co-transfected with pMK467 (dTAG-BromoTag-mAID-EGFP-NLS) and pCS-TP (TOL2 transposase) with the Neon system (Thermo Fisher Scientific) (Sumiyama et al ...
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bioRxiv - Cell Biology 2024Quote: ... 2 (ThermoFisher Scientific; 4390846), CCDC51 oligo #1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and DNM1L/Drp1 (Thermo Fisher Scientific; s19560). CCDC51 oligo #1 was used in all experiments unless stated otherwise ...
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bioRxiv - Cell Biology 2024Quote: ... CCDC51 oligo #1 (Thermo Fisher Scientific; s36162), CCDC51 oligo #2 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were carried out with Lipofectamine 3000 (ThermoFisher Scientific) according to manufacturer’s instructions for 5-6 hours ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 25nM MitoTracker CMXRos (Thermo Fisher Scientific; M7512) in DMEM for 30-60 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... CCDC51 oligo #2 (Thermo Fisher Scientific; s36164), and DNM1L/Drp1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... transfections were performed in 6-well plates with Lipofectamine RNAiMAX (ThermoFisher Scientific) and 20 nM RNAi oligonucleotides according to manufactuer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% penicillin-streptomycin (Invitrogen)) was used for cell culture ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse anti-FAK (ThermoFisher Scientific; 34Q36), Rabbit anti-phospho-FAK (Y925 ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were analyzed by reversed-phase nLC-MS/MS using an Ultimate 3000 UHPLC coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18 ...
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bioRxiv - Cell Biology 2024Quote: ... MS/MS fragment spectra were searched using Sequest HT in Proteome Discoverer (Thermo Scientific) against a human database (UniProt ...
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bioRxiv - Molecular Biology 2024Quote: ... Reporter stable cell line was generated by transfection (Lipofectamine™ 2000, thermofisher) and drug selection (Hygromycin, Thermofisher).
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bioRxiv - Molecular Biology 2024Quote: ... and 50 μM β-mercaptoethanol (Gibco, 21985023). To generate Ogt iKO mESC carrying the 2C-reporter we used the MERVL::tdTomato reporter [51] that was kindly shared from Samuel Pfaff (Addgene #40281) ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibiotic selection was performed by culturing the transfected mESC with 1 um puromycin (Thermo Fisher, A1113803) for 2 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... sfGFP::CENP-A were acquired from the Needleman Lab (Harvard University) and cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... All libraries were assessed using Qubit™ RNA HS Assay Kit (ThermoFisher, Q32855) and TapeStation High Sensitivity RNA ScreenTape Analysis (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... AHR- or OVOL1-specific siRNA (generated by Huzhou Hippo Biotechnology Co., Ltd) was transfected into NHEKs using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific, Cat# 13778075) per manufacturer’s protocol.