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Citations for Thermo Fisher :
9151 - 9200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and western blot carried out using the iBind Western Blot Apparatus (Life Technologies). Affinity-purified rabbit polyclonal antibodies specific to each eIF4E family member (Genscript ...
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bioRxiv - Molecular Biology 2024Quote: ... 2mM L-glutamine (Thermo Fisher Scientific, 25030081), 2 mM sodium pyruvate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... deposited by Blau H.M.) and the secondary antibody was an Alexa Fluor 488-conjugated anti-mouse antibody (Invitrogen). Nuclei were counter-stained with DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... non-essential amino acids (Thermo Fisher Scientific, 11140050), 2mM L-glutamine (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... a 20μl system of ChamQ SYBR Master Mix (Low ROX Premixed) (Q331, Vazyme, China) is employed on the ABI QuantStudio5 System(Thermo Fisher, USA). GAPDH is chosen as the internal reference gene ...
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bioRxiv - Molecular Biology 2024Quote: ... transfection (2μg plasmid) followed by 500 μg/ml hygromycin B (Gibco, 10687010) selection for 10 days before being single-cell sorted ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM sodium pyruvate (Thermo Fisher Scientific, 11360070), and 100 μM 2-mercaptoethanol (MP Biomedicals ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Bolt Mini Blot Module (Life Technologies) and western blot carried out using the iBind Western Blot Apparatus (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was cut into smaller pieces and placed into gelatin-coated tissue culture dishes with DMEM (Thermo Fisher Scientific, 10567022) supplemented with antibiotics ...
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bioRxiv - Molecular Biology 2024Quote: ... where targeted vision genes were PCR-amplified in 16 cycles after capture with streptavidin beads (Dynabeads, ThermoFisher). Once amplified ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified by phenol/chloroform (pH 7.0) (ThermoFisher, 10308293) and ethanol precipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 1μL RNase A (10 μg/ml) (Thermo Scientific™, EN0531) and 2 μL Proteinase K (10 mg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... or mock were cultured in DMEM media (Gibco, #11965-084) supplemented with puromycin and 10% FBS (Atlas Biologicals ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1µL/sample Turbo DNase in 1X Turbo DNase Buffer (100µL/sample)(Thermo fisher, AM2238) was added to digest chromatin pellets at 37°C with 1000rpm shake until pellets were dissolved ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclear lysates of inputs and affinity-purified elution were loaded on a 4-20% Tris-glycine gel (Invitrogen, Cat# XP04205BOX), 10% acetic acid and 50% methanol was used to fix the gel in for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 11873580001)/1X phosphatase inhibitors (Thermo Fisher, A32961)(PPI)] and 1μL/sample of Benzonase® Nuclease (Merck ...
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bioRxiv - Microbiology 2024Quote: ... and EDTA-free protease inhibitor cocktail (Thermo Fisher J61852.XF). Parasites were lysed by 10 passages through a syringe fitted with a 25-gauge needle and 20 passages with a 27-gauge needle ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were resolved by SDS-PAGE and transferred to a nitrocellulose membrane using the iBlot Dry Blotting System (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... NRK cells and HEK 293FT cells were grown in DMEM (Gibco, C11995500BT) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... 1% (v/v) glutamax (Gibco, 35050-061) and 1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... ESCs were electroporated with the linearized vector using XhoI and selected in 150 μg/mL G418 (Thermo Fisher Scientific). Clones were validated by genomic PCR using the following primers ...
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bioRxiv - Cell Biology 2024Quote: Equal amounts of cells were lysed in 1X LDS sample buffer (Life Technologies) containing 10% NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% streptomycin and penicillin (GIBCO, 15140122). The cells were grown under conditions of 37°C with 5% CO2 and tested for mycoplasma regularly.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA assay (Thermo Scientific Scientific) and samples were diluted in lysis buffer (50 mM Tris pH 6.8 ...
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bioRxiv - Molecular Biology 2024Quote: Cells expressing Corelet system constructs (NLS-iLID-GFP-Ferritin and either BRD4dN-mCh-sspB or BRD4FL-mCh-sspB) via lentiviral transduction were seeded on 96-well glass bottom plates (ThermoFisher Cat. No NC0536760), then loaded onto a Nikon A1 laser scanning confocal microscope with Nikon Eclipse Ti2 body ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies used were Alexa Fluor 488 anti-rabbit (A-21206; Thermo Fisher Scientific; 1:200), Alexa Fluor 488 anti-mouse (A-21202 ...
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bioRxiv - Cell Biology 2024Quote: ... males were picked onto an OP50 plate and treated with 200 uL 0.05 mM working stock of MitoTracker Deep Red FM (Invitrogen) or MitoTracker Red CMXros (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... or MitoTracker Red CMXros (Invitrogen) in M9 overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies used in this work were mouse monoclonal anti-tubulin (DM1α; Thermo Fisher Scientific; 1:200) and rabbit anti-GFP (NB600-308 ...
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bioRxiv - Cell Biology 2024Quote: ... and Alexa Fluor 594 anti-mouse (A-21203; Thermo Fisher Scientific; 1:200). Z-stacks were captured at 1-μm steps for meiotic embryos using the same microscope described above for live imaging.
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bioRxiv - Cell Biology 2024Quote: ... and/or 0.2U/mL Alexa Fluor 647-labeled phalloidin (Invitrogen) for 35-45min as detailed in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... coverslips were mounted on glass slides in ProLong Gold anti-fade (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 488 anti-mouse (A-21202; Thermo Fisher Scientific; 1:200) and Alexa Fluor 594 anti-mouse (A-21203 ...
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bioRxiv - Cell Biology 2024Quote: ... 1mL Glutamax (Gibco-#35050-061), 4.4mg Vitamin C (Sigma-#A4403) ...
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bioRxiv - Cell Biology 2024Quote: ... 1×106 mESCs/sq.cm plated with KnockOut DMEM (Gibco-10829-018) supplemented with 2% FBS and incubated overnight at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... 1X Penicillin/streptomycin (Gibco-#15240-062), make up to 100mL with MCDB (Sigma-#M6770)] with 100ng/mL Activin (Sino Biological-#10429 ...
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bioRxiv - Cell Biology 2024Quote: ... with UNG (ThermoFisher, Waltham, MA, Cat. no. 4440038), was used to perform qRT-PCR of cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... or 647 nm depending on the incorporated fluorophore using a Nanodrop™ 2000 spectrophotometer (Thermo Fisher Scientific). To assess the quality of generated probes ...
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bioRxiv - Cell Biology 2024Quote: ... The cell suspension was diluted 10-fold with EMM2 liquid medium without thiamine in a 96-well DeepWellTM plate (ThermoFisher, #260251), and this was subjected to incubation at 33°C for 6 h with shaking at 900 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 2000 (Thermo Fisher Scientific), as we have previously described (46,49,50) ...
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bioRxiv - Cell Biology 2024Quote: ... washed twice with Opti-MEM (Thermo Fisher scientific) and resuspended in BTXpress solution (BTX) ...
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bioRxiv - Cell Biology 2024Quote: ... 1× B-27 Supplement (Life Technologies), 1.25 mM N-acetylcysteine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was measured in triplicate with the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific). Immunoblotting experiments were performed as already described(Notarangelo et al. ...
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bioRxiv - Cell Biology 2024Quote: ... pATM-Ser1981 (1:1000, Invitrogen), pATR-Thr1989 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were Alexa-fluor 488 anti-mouse IgG (1:1000, Invitrogen), Alexa-fluor 555 anti-rabbit IgG (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then stained with fluorescently labeled wheat-germ agglutinin (1:1000, Invitrogen) to stain lectin and nuclei were labeled with DAPI (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... Otx2 (1:1000, Invitrogen) and GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... pATM-Ser1981 (1:500, Invitrogen) and pATR-Thr1989 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa-fluor 555 anti-rabbit IgG (1:1000, Invitrogen). Cells were then stained with fluorescently labeled wheat-germ agglutinin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... these cells were isolated from the gels using TrypLE Express (Gibco). 10⁵ cells per ml were suspended in PBS and were mixed with 1% low melting agarose (GeneI ...