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Citations for Thermo Fisher :
9201 - 9250 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the plate was washed 4 times with PBST using an automated plate washer (Thermo Fisher Scientific Wellwash Versa, USA), and the samples were incubated in anti-pTRKB antibodies diluted 1:1000 in the blocking buffer overnight at 4° C ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 4 seconds at -2 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher). The blotting chamber was maintained at 22°C and 100% humidity during freezing.
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bioRxiv - Molecular Biology 2021Quote: ... and dialyzed against PBS (pH 7.4) at 4°C overnight using a SnakeSkin Dialysis Tubing (Thermo Fisher Scientific, 68100). The RBD was further polished by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2021Quote: ... For PhoX crosslinking we used a Fe3+-IMAC column (Propac IMAC-10 4 × 50 mm column, Thermo Fisher scientific) connected to an Agilent HPLC ...
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bioRxiv - Biochemistry 2020Quote: ... Protein gels were run using sodium dodecyl sulfate polyacrylamide gradient gels (NuPage 4−12% Bis-Tris gel from Invitrogen) using the BenchMark protein ladder (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... After 4 washes with PBS 0.01% v/v Tween-20 and 2 washes with ELISA Light washing buffer (ThermoFisher), CSPD substrate with Sapphire II enhancer (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... The chromatin was then cleared by centrifugation (12,000 g for 10 minutes at 4°C) and 80 μL of protein G DynaBeads (ThermoFisher, washed 3 times in cold ChIP buffer right before use ...
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bioRxiv - Microbiology 2020Quote: Proteins were separated by SDS-PAGE using NuPAGE 4–12% (w/v) Bis-Tris gradient gels (Thermo Fisher Scientific), and then transferred onto a PVDF membrane using the iBlot 2 dry blotting system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... over 4 independent replicates of cultures in plates (96 wells; Nunclon 162 TM Surface; Nunc A/S, Roskilde, Denmark). Plates were incubated at 30 °C for 48 h with continuous orbital shaking at 180 rpm ...
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bioRxiv - Microbiology 2020Quote: ... and the wells were overlaid with 4 ml/well DMEM with 5% FBS and 0.7% agarose (Invitrogen, 16500-500). At 6 d.p.i. ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using a Gel Extraction Kit (OMEGA, D2500-01) and quantified with Qubit 4 fluorometer (Invitrogen). The amplicon library was sequenced by Illumina MiSeq (500-cycle ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were then incubated for 120 minutes with anti-Rab14 (4 µg/ml in 0.1% (v/v) horse serum (Gibco), 0.1% (w/v ...
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bioRxiv - Neuroscience 2020Quote: All proteins were separated on pre-cast 4-12 % Bis-Tris SDS-PAGE gels (NPO321BOX, NPO322BOX, NPO336BOX) from Invitrogen using MES running buffer (50mM MES ...
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bioRxiv - Pathology 2021Quote: A section of the infected lung tissues (4 dpi) was collected in 0.5 mL of TRIzol LS reagent (ThermoFisher) in 2 mL tubes with a 5mm bead ...
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bioRxiv - Immunology 2020Quote: ... cells were permeabilized overnight at 4°C with the Foxp3/ Transcription Factor Staining Buffer Set according to the manufacturer’s instructions (ThermoFisher).Cell fluorescence was measured using an LSRII flow cytometer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10-100 μg total protein was then separated with a 4-12% (v/v) gradient Bis-Tris gel (ThermoFisher) run in MES buffer system ...
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bioRxiv - Molecular Biology 2020Quote: ... 1,1’-bi(4-anilino) naphthalenesulfonic acid (Bis-ANS) and Alexa Fluor® 488 phalloidin were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 20000xg for 30 minutes at 4°C before supernatant is removed and addition of 0.5% G-250 sample additive (Invitrogen). First dimensional BN-PAGE was run on NativePAGE™ 3-12% Bis-Tris gels under native conditions at 150V for 30 minutes with 1x native cathode buffer (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were transfected at DIV3-4 (knock-in constructs) or DIV10-11 (overexpression constructs) using Lipofectamine 2000 reagent (Invitrogen). Shortly before transfection ...
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bioRxiv - Synthetic Biology 2021Quote: ... the insert with 4 × NNK library was amplified by Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher Scientific) with the following primers ...
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bioRxiv - Immunology 2021Quote: ... Libraries were quantified using a Qubit 4 Fluorometer and the Qubit dsDNA HS assay kit (ThermoFisher Scientific, Waltham, Massachusetts). The QIIME2 platform was used to process sequence data ...
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bioRxiv - Immunology 2021Quote: ... Samples were resolved by electrophoresis on 4-12% Bis-Tris gels with MOPS Running Buffer (Life Technologies NuPAGE system) and transferred to nitrocellulose membrane by electrophoresis at 100V for 1 hour in cold transfer buffer (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: Cell lysates and immunoprecipitations were resolved using precast Novex 4-12% Bis-Tris Midi Protein Gels (Thermo Fisher Scientific) and transferred to methanol-activated Immobilon-P PVDF Membranes (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen) and images were acquired using Nikon A1-R confocal microscope using the NIS Elements software ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The heart and adjacent posterior body wall structures were isolated and stained in 2.5 mmol/l di-4-ANEPPS (Invitrogen) for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... for 12 h at 4°C and then Alexa Fluor 647-conjugated goat anti-rabbit (Thermo-Fisher Scientific Inc.) for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Thirty micrograms of protein lysates were resolved on Bolt 4-12% Bis-Tris plus gel (Invitrogen, Carlsbad, CA, USA) under reducing conditions and transferred to a nitrocellulose filter ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were washed in 0.1% IGEPAL/PBS and mounted using ProLong Gold antifade reagent with 4’-6-Diamidino-2-phenylindole (DAPI) (Invitrogen). Immunofluorescence studies in HeLa cells were carried out as described above ...
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bioRxiv - Immunology 2019Quote: Twenty microgram (µg) of total protein lysates were size-fractionated on 4-12% NuPAGE Bis-Tris gel (#NP0321, Invitrogen). OLR1 was detected by incubation with a rabbit anti-mouse OLR1 antibody (1:500 ...
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bioRxiv - Plant Biology 2019Quote: ... loaded on leaf area basis and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Cell Biology 2019Quote: ... Mounting medium contained DAPI (4’, 6-diamidino-2-phenylindole) to visualize the nucleus (Molecular Probes Inc., Eugene, OR, USA). Negative controls were performed with either no primary or no secondary antibodies ...
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bioRxiv - Genomics 2021Quote: ... we extracted total RNA from rosette leaves of 4-5-week-old plants using Trizol (Invitrogen, cat. No. 15596026) according to manufacturer’s manual ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and the eluate was loaded on a 4-12% NuPAGE Bis-Tris gel (Invitrogen). 0.5 l of 1x MOPS running buffer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: For Diff-Quik staining HLA-DR+ cells were centrifuged onto glass slides in Cytospin 4 cytocentrifuge (Thermo Fisher Scientific). Cells were fixed with methanol ...
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bioRxiv - Neuroscience 2021Quote: ... incubated 5 minutes with at room temperature with 500 ng/ml 4’,6-Diamidino-2-Phenylindole Dilactate (DAPI; ThermoFisher) in DPBS and mounted in ProLong®Gold (ThermoScientific).
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bioRxiv - Cell Biology 2020Quote: ... The immunoprecipitates were dissolved in 2×SDS loading buffer and resolved on NuPAGE 4–12% Bis-Tris gel (Invitrogen), and then silver stained using Pierce silver stain kit (Thermo) ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... 2008) overnight at 4°C and then incubated with anti-mouse secondary antibodies labeled with Alexa fluor 488 (Invitrogen) for 1 hour at room temperature and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Microbiology 2020Quote: ... solution and spun for 1.25 h at 41,500 x g at 4°C in a Sorvall S55-A2 fixed angle rotor (ThermoFisher Scientific). Cell and virus fractions were lysed in lysis buffer (30 mM NaCl ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation, Carlsbad, CA). Proteins were transferred to a nitrocellulose membrane and probed against photosynthetic proteins with antibodies according to manufacturer’s protocols (Agrisera ...
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bioRxiv - Cell Biology 2019Quote: ... All siRNA transfections (see table 4 for siRNA sequences) were performed with 40 siRNA duplexes using Lipofectamine RNAiMAX (Invitrogen) in OptiMEM without FBS.
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 4% formalin solution (neutrally buffered) and kept in phosphate buffered saline (PBS, Gibco no. 10010023) during the experiment.
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Fetal guinea pig muscle cells were cultured for 48 hrs in 4-well chamber slides (Nunc; Thermo Scientific, Canada) and then fixed for 10-15 min using 4% paraformaldehyde ...
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bioRxiv - Cell Biology 2020Quote: ... Fetal guinea pig muscle cells were cultured for 48 hrs in 4-well chamber slides (Nunc; Thermo Scientific, Canada) and then fixed for 10-15 min using 4% paraformaldehyde ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were run at 4 °C for 90 min and then stained 30 min with SYBR™ Gold (ThermoFisher). Gels were imaged on a fluorescence Typhoon imager with the excitation and emission set to 480 nm and 520 nm ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed with PBS and then fixed with 4% paraformaldehyde (Thermo Fisher Scientific Life Technologies, Waltham, MA) at room temperature for 10 minutes ...
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bioRxiv - Genetics 2021Quote: ... were taken to reducing sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE; Gels: ThermoFisher NuPAGE 4 to 12 %, Bis-Tris). The separated proteins were transferred to nitrocellulose membranes (0.2 µm ...