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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... A small fraction of prospectively isolated cells was cytospun onto slides immediately after isolation with Cytospin 4 (Thermo Scientific) at 500 rpm for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX, ThermoFisher) and then transferred onto a Biotrace NT nitrocellulose membrane (27376-991 ...
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bioRxiv - Cancer Biology 2022Quote: ... Mesenteries were then stored for up to 48hrs at 4°C into PBS with 2% Antibiotic-Antimycotic solution (ThermoFisher).
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bioRxiv - Biophysics 2022Quote: ... DRGs were dissociated and plated at a density of 4 × 104 per chamber (Lab-Tek coverglass system Nunc #155411), coated with Matrigel (Becton Dickinson ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody, # PA1-16972, Invitrogen) diluted 1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Each sample was analyzed with a 4 hour gradient using a 50 cm EASY-Spray column (Thermo Scientific; Dionex). Raw MS/MS files were analyzed with the MaxQuant software ...
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bioRxiv - Bioengineering 2022Quote: ... lungs were rinsed with antibiotics/antimycotics (10% P/S, 4% amphotericin-B [Sigma], 0.4% gentamicin [Gemini Bio] in PBS with Ca2+ and Mg2+ [Gibco]) followed by perfusion with 0.0035% Triton X-100 (American Bioanalytical) ...
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bioRxiv - Cell Biology 2022Quote: ... Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen). Crypts were collected and filtered through 70 µm cell strainers in subsequent fractions ...
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bioRxiv - Neuroscience 2022Quote: ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using precast 4-12% polyacrylamide gradient gels (ThermoFisher, NW04122BOX), submerged at 4°C in Bolt™ MOPS SDS Running Buffer (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: The lysates were analyzed by 4-16% Native PAGE using precast Bis-Tris Mini Protein Protein Gels (Invitrogen BN1003BOX) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... at 69028 RFC in 4◦C for 30min and the cleared lysate was aspirated and added 1µl DNase (ThermoFisher). Further purification of the CrRabL2 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
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bioRxiv - Biochemistry 2022Quote: ... Forty μg of protein extract for each sample were separated on a 4–12% NuPage Bis-Tris gel (Invitrogen) at 125 V for 100 min in NuPage MES SDS Running Buffer (Invitrogen)and transferred to nitrocellulose membranes.
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bioRxiv - Biochemistry 2022Quote: ... Fifteen to 20 μg of protein was loaded into a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies) and transferred onto a nitrocellulose membrane with the iBlot 2 semidry transfer system (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 mM aminocaproic acid and 30% sucrose) and loaded onto a 4-16% precast BN-PAGE gel (Life Technologies). Second dimension analysis was performed by solubilizing BN-PAGE gel slices in 2x Laemmli buffer (Laemmli ...
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bioRxiv - Microbiology 2022Quote: ... The PCR mixture (20 μL of final volume) consisted of 4 μL of buffer Phusion High Fidelity 5X (ThermoFisher) (1X final) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed for 30 min at 4°C using eBioscicencesTM FoxP3/Transcription factor staining buffer set (Thermo Fisher) and stained for the following intracellular markers ...
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bioRxiv - Genetics 2022Quote: Denatured protein lysates (12.5 μg/sample) were loaded on to NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gels (Invitrogen, NP0321) alongside Chameleon Duo Protein Ladder (3 μL/lane ...
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bioRxiv - Bioengineering 2022Quote: ... glacial acetic acid) for 2 hours at room temperature (Section 2.14) or methanol-free 4% formaldehyde (ThermoFisher, Section 2.16) and then kept in PBS at 4°C before washing in gradations of ethanol up to 100% ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were mounted with the ProLong™ Diamond Antifade Mountant with 4’,6-Diamidino-2-Phenylindole (DAPI) (ThermoFisher Scientific). Ki-67 signals were visualized with Nikon microscopy ...
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95°C for 5 min before loading onto a NuPAGE Bis-Tris 4-12% gel (Invitrogen). Immunoblotting was performed using a Mini Trans-Blot cell (BioRad ...
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bioRxiv - Biophysics 2022Quote: ... NaV1.4 (GenBank: MZ545381.1) expressed in a pCDNA3.1 vector (9) was made using the mMACHINE™ T7 Transcription Kit (Invitrogen). Xenopus oocytes were injected with 3– 6 ng of Pt NaV1.4 and TEVC experiments were performed 1–2 days post-injection ...
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bioRxiv - Biophysics 2022Quote: ... 0 to −4 blot force range) and plunge-freezing in liquid ethane using a Vitrobot Mk IV (Thermo Fisher).
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bioRxiv - Genomics 2022Quote: ... and lysates were cleared by centrifuging at 11,000 × g for 15 min at 4°C and quantified using Qubit reagent (Invitrogen). For preparation of samples for SDS PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... The grids were blotted for 4 s and plunge-frozen in liquid ethane with a Vitrobot Mark IV (ThermoFisher) at 4°C and 100% humidity ...
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bioRxiv - Genomics 2022Quote: ... 3C libraries were ligated for 4 hours at 25ºC with 10U T4 ligase and ligation buffer (ThermoFisher cat 15224) in a thermomixer at 1000rpm ...
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bioRxiv - Immunology 2022Quote: SDS-PAGE was performed with 30 μg of protein lysate on 4– 12% NuPAGE Bis-Tris (Thermo Fisher Scientific), in MOPS buffer as described [23] ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspension was clarified by centrifugation at 16,000 g for 10 min at 4 °C The clarified protein lysate was mixed with 1.5 mg magnetic beads (Dynabead M-270 Epoxy, ThermoFisher) conjugated with in-house GFP nanobody (Fridy et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were centrifuged at 20,627g for 20 min at 4°C and incubated with Neutravidin agarose beads (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were centrifuged at 20,627g for 20 min at 4°C and incubated with glutathione agarose beads (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Coverslips were affixed using Prolong Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, Cat. # P36935).
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bioRxiv - Plant Biology 2021Quote: ... The samples were precipitated with anti-Myc antibody and ran on a 4-12% NuPAGE Bis-Tris gel (ThermoFisher) and blotted using the MiniBlot Module (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... brains were transferred through 4 wells containing a blocking solution (5% Goat Serum in PBT; Life Technologies #16210-064), and sitting in the last well for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... DNA was quantified and quality checked using a Qubit 4 Fluorometer and a Nanodrop 2000 Spectrophotometer (Thermo Scientific™). DNA metagenomic libraries were generated using the KAPA Library System (per manufacturer’s instructions) ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in 100 µL of a 0.5% saponin solution containing 4 µg rabbit α-TMPRSS2 (Invitrogen #PA5-14264). After 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Eluates were collected from the beads by centrifugation and resolved on a NUPAGE 4-12 % Bis-Tris gel (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... a PDL-coated mylar film dish was biotinylated by incubating NHS-biotin (Thermo Fisher Scientific, 200 4 µg/ml) for 3h ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... pelleted by centrifugation at 1,000 rpm for 4 min and resuspended in sorting buffer containing HBSS (Gibco, #14175-079), 2% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromatin was clarified by centrifugation at 21,000 g at 4°C for 10 min and precleared with 80 µl prewashed Dynabeads protein A (ThermoFisher) for 30 min at 4°C ...
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bioRxiv - Genetics 2021Quote: ... for 10 minutes at 70°C before loading into Bolt 4-12% Bis-Tris Plus gels (Thermo Fisher Scientific). Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Library quantity and quality were assessed with Qubit 4 Fluorometer with the 1X dsDNA HS Assay Kit (Invitrogen Q33231) and on a Bioanalyzer 2100 System with a High Sensitivity DNA chip ...
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bioRxiv - Molecular Biology 2020Quote: ... for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... gel lanes from BN gels were placed onto top of a 4-12% NuPage 2D well (#NP0326, Thermo Scientific). After separation and blotting ...
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bioRxiv - Neuroscience 2020Quote: ... and the samples were first post-fixed overnight (4°C) and then placed in 0.25 M EDTA (Invitrogen AM9261) in PBS at 4°C for 3 days for decalcification ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were centrifuged for 16h at 100 000xg at 4°C (TH-641 Sorvall; Thermo Fisher Scientific, MA, USA) and afterwards 1mL fractions were collected starting from the top to bottom ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein from extracts were separated in SDS–polyacrylamide gels 4–12% (NuPAGE Bis-Tris, Thermo Scientific) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of protein for each sample were loaded and run on a 4–12% SDS-PAGE gel (Invitrogen). A nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... After washing they were incubated overnight at 4°C with Alexa 555 Phalloidin anti-F-actin (A34055, ThermoFisher Scientific) and the secondary antibodies diluted in 0.3% PBT ...
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bioRxiv - Microbiology 2020Quote: ... and separated by SDS-PAGE using 16% Tricine protein gels or NuPAGE 4-20% Bis-Tris gels (Thermofisher Scientific) and transferred to nitrocellulose membranes using the iBlot™2 Dry Blotting system (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were then transferred onto fresh media for 4 hours and immediately placed in RNAlater solution (Ambion™). Experiments were carried out in biological duplicates ...