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Citations for Thermo Fisher :
9251 - 9300 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... which were boiled for 5 min at 95°C and then separated on 4-12% polyacrylamide gradient gels (Invitrogen). After blotting ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated by electrophoresis in NuPAGE 4-12% Bis-Tris gels run with 1x MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were acidified with 4 μL of 50 % formic acid and dried in a SpeedVac centrifugal evaporator (ThermoFisher) at 65°C for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were immersed in 2ml dissociation solution (2% FCS-PBS solution with approximate 145U/ml type 4 Gibco collagenase) and incubated at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor specimens were minced and digested for up to 4 h at 37°C in AdMEM/F12 medium (Gibco) containing 2 mM GlutaMAX (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... 20μg of protein were denatured in loading dye containing SDS then resolved by 4-12% SDS-PAGE (Invitrogen #NP0336BOX). Protein was transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein was loaded into a 15-well NuPAGE 4-12 % Bis-Tris gel (Cat# NP0336BOX, ThermoFisher) and run in MES buffer (50 mM MES ...
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bioRxiv - Cell Biology 2020Quote: ... along with the pOGG4 (4 µg) plasmid were transfected into cells using 7.5 µL of Lipofectamine 3000 (ThermoFisher, L3000001). All transfections were performed in 6-well culture dishes at 80% confluency ...
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bioRxiv - Microbiology 2020Quote: ... and 5µl of the diluted samples were loaded onto a 4-12% bis-tris NuPage gel (Thermo Fisher Scientific) and run at 200V for 50min in MOPS buffer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: 4 μl of purified spores (6×107 spores/ml) were mixed with 40 μl of NucBlue (Invitrogen, catalog #R37605) and incubated at 25°C for 40 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were subjected to size selection (250–500 bp) on a 4–20% TBE PAGE gel (Thermo Scientific). The recovered libraries were sequenced on the Illumina Nextseq platform using Nextseq 500 mid-output kit with 2×150 paired-end.
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were subjected to size selection (250–500 bp) on a 4–20% TBE PAGE gel (Thermo Scientific). The recovered libraries were sequenced on the Illumina Nextseq platform using Nextseq 500 high-output kit with 2 × 76 paired-end ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Ovaries were washed 4 times for 10min in PBS-Tr and stained with 0.5µg/ml DAPI (Thermo Fisher Scientific) for 10min ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 2 μg of protein per lane was loaded on a 4-12% graded Tris-glycine SDS-polyacrylamide gel and proteins were transferred to a 0.2μm PVDF membrane (Life Technologies). Membranes were blocked in 5% milk in PBS with 0.05% Tween-20 for one hour at room temperature with rocking ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR was performed with standard procedures on 4 μg total RNA with 200 u M-MLV RT (Invitrogen), using the primers listed in Table SX ...
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bioRxiv - Immunology 2021Quote: ... lysed in 1X Novex buffer and 5-7.5×105 cells equivalents were resolved on 4-12% NuPAGE gel (Invitrogen) in MOPS buffer ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated with 0.2 μM of 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Life Technologies, Carlsbad, CA) in PBS pH 7.4 ...
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bioRxiv - Genomics 2020Quote: ... standards (3 x 10μl) and samples (6 x ∼18μl) were loaded on a 4-12% Bis-Tris Nu-PAGE (Life Technologies) gel for electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... or with Aurora B-FRET sensor (CFP/YFP) (Fig. 2g-l, Extended Data Fig. 4) or alternatively by labelling with Hoechst 33342 (1.62 μM, Invitrogen) (Figs ...
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bioRxiv - Immunology 2021Quote: ... washed and stained with LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (20 mins, 4°C; Thermofisher, #L34965). After washing ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 4% formalin solution (neutrally buffered) and kept in phosphate buffered saline (PBS, Gibco no. 10010023) during the experiment.
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bioRxiv - Immunology 2022Quote: ... The soluble fraction was supplemented with 0.5% Coomassie G-250 immediately before loading samples onto NativePAGE 4 to 16% Bis-Tris mini gels from Invitrogen™ ...
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bioRxiv - Neuroscience 2022Quote: ... OPC-DRG neuron cocultures on coverslips were loaded with 4 μM of fura-2AM (Life Technologies, Grand Island, NY) for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting survivor cells were expanded for 4 days before transferring to a T25 cell culture flask (Thermo Fisher). During the transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... quantified using aLife Technologies Qubit 2.0 instrument were run on 4-12% Bis-Tris Plus Bolt gels (ThermoFisher Scientific) and transferred to a nitrocellulose membrane with an iBlot dryblot system (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Then the cells were fixed with 4% paraformaldehyde solution and analyzed with Attune NxT flow cytometer (Thermo Fisher, MA) at UNC Flow Cytometry Core Facility ...
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bioRxiv - Cell Biology 2022Quote: ... Briefly 24 pmol siRNA was used together with 4 µl of liposomal transfection reagent in 400 µl OptiMEM (Invitrogen) per 3×105 cells and scaled accordingly ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were heated to 95°C for 10 min and run on NuPAGE 4%-12% bis-tris gels (Invitrogen). Proteins were transferred to PVDF membranes (BioRad ...
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bioRxiv - Immunology 2022Quote: ... Dynabeads™ Mouse T-Activator CD3/CD28 beads were added according to manufacturer’s instructions for 4 hours (Thermo Fisher). At the conclusion of 4 hours ...
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bioRxiv - Immunology 2022Quote: ... cells were centrifuged at 4°C at 200xg for 10 minutes and fixed with IC fixation buffer (Thermo Fisher) in 200 µL (100 µL IC Fixation buffer + 100 µL PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... were added to the wells and ran on a NuPage™ 4-12% Bis-Tris mini gel (Invitrogen, NP0336BOX). After gel electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Untransfected Flp-In T-REx HEK293 cells were cultured under selection with 4 µg/mL blasticidin (Gibco, ThermoFisher Scientific) and 50 µg/mL zeocin (Invitrogen ...
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Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteinsbioRxiv - Biochemistry 2022Quote: ... 4 μl of the flow through were subjected to a protein determination (Pierce BCA Protein Assay (Thermo Scientific, #23225). 25 μl of the remaining flow-through were mixed 1:1 with 2x sample buffer (180 mM Tris pH 6.8 ...
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bioRxiv - Biochemistry 2022Quote: ... Grids were blotted (3s, force 4) and rapidly plunge-frozen in liquid ethane using a Vitrobot (Thermo Fisher Scientific) in 100% humidity at 22 ℃ ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Cell Biology 2022Quote: ... was added to the samples and the samples transferred to a NuPAGE 4-12% Bis-Tris Gel 1.0 mm x 10 well (Invitrogen) for SDS PAGE gel electrophoresis ...
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bioRxiv - Microbiology 2022Quote: ... Transcription reactions were incubated 4 h at 37 °C and transcripts were purified with TRIzol Reagent (Thermo Fisher Scientific) and Direct-zol RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2022Quote: siRNAs purchased from Eurofins MWG Operon or Sigma-Aldrich (Table 4) were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates were then cleared by centrifugation at 40,000g at 4 °C for 20 min and flowed through columns packed with protein-A Sepharose beads (Invitrogen) to deplete the endogenous antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were cultured on glass coverslips in a 4-well plate containing Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, 12320032) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: The cells were stained with 29 μM DAE620-Mito for 4 hours and 100 nM MitoTracker Green (Invitrogen, M7514) for 30 minutes in the culture medium ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were lysed in 300 µL SDS buffer (50 mM HEPES pH 7.4, 10 mM EDTA pH 8.0, 4% UltraPure™ SDS Solution (24730020, Invitrogen), freshly added 1× cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Biophysics 2022Quote: ... thoracic and lumbar DRGs were harvested from 4 to 6 week old MrprD-GFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and these plasmids were combined with the appropriate destination vectors (see Table 4) in LR Clonase II reactions (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... NCC primary cultures were performed in 4-well plates or in 8-well Chambered Coverglass (Nunc, cat. no. 154511) coated with fibronectin ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg protein of each sample was loaded into 4 to 12% Bis-Tris protein gel (Thermo Fisher Scientific) and sent for MS analysis at the University of Texas System Proteomics Network ...
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bioRxiv - Developmental Biology 2022Quote: ... for 2 hours at 250mA and 4°C using cold transfer buffer (1.5x NuPAGE transfer buffer (Thermo Fisher Scientific), 10% methanol ...