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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 105 – 4 x 105 mESCs were cultured in 60mm Petri dishes in DFNB medium (Neurobasal medium/Gibco, DMEM F12 1:1/Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent indicators (fluo-4 AM, fura-2 AM, fura-FF AM) were purchased from Molecular Probes (Thermo Fisher Scientific). NMDA was from Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... 150 µg of protein extract from each sample were ran on NuPAGE 4%–12% Bis–Tris Protein Gels (Invitrogen) using MES running buffer (100 mM MES ...
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bioRxiv - Cell Biology 2022Quote: ... images of live cell morphology were captured using the epifluorescence mode and with FM™ 4-43 (Invitrogen, T3166) added right before imaging.
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bioRxiv - Neuroscience 2021Quote: ... Cell transfections were carried out in 96-well plate with 1.0×10^4 target cells where each well received 100 ng DNA with a mix of Lipofectamine 3000 (Invitrogen) in Opti-MEM.
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle overnight at 4 °C (Alexa flor 488 goat anti mouse, Invitrogen A11001 ...
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bioRxiv - Neuroscience 2019Quote: ... 25µg of total protein (60µg for PLCγ1) in 20µL was run on 4-20% tris-glycine gels (ThermoFisher #XP04205BOX, USA) for 120 minutes at 130V ...
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bioRxiv - Molecular Biology 2020Quote: ... were resolved on precast NuPAGE 4-12% Bis-Tris midi 12+2-well protein gels (cat. no. WG1401BOX, Invitrogen) at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted in ProLong™ Gold Antifade Mountant containing 4’,6’-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). Images were acquired at 100x magnification using a Nikon Eclipse Ti fluorescence microscope fitted with a Hamamatsu C11440 digital camera ...
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bioRxiv - Molecular Biology 2020Quote: ... ∼50 µg of protein extracts was then resolved on precast NuPAGE Novex 4–12% Bis-Tris gels (Invitrogen, NP0321BOX). The proteins were transferred to a nylon membrane with the semidry transfer Pierce Power System (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The endogenous antibodies were added for 4 °C overnight reaction before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Genomics 2020Quote: ... The samples were genotyped using a 200K Affymetrix Axiom® myDesign Custom SNP Array developed by the EPIC4 coho salmon genome consortium (http://www.epic-4.org) and built by ThermoFisher Scientific (San Diego ...
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bioRxiv - Neuroscience 2020Quote: ... Sample volumes were adjusted with RIPA buffer containing 100 mMDTT and NuPAGE LDS Sample Buffer 4× buffer (Life Technologies) and boiled for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was incubated at 4 °C for 30 min with 60 μl of Dynabeads M-270 Streptavidin (Invitrogen) equilibrated with lysis buffer containing 1% Triton X-100 ...
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bioRxiv - Cell Biology 2019Quote: Between 40-50µg of samples were run on NuPAGE Bis-Tris 4–12% gels in NuPage MES SDS Buffer (Invitrogen) for 40 minutes at 200 V and transferred to 0.4-µm nitrocellulose membranes using a semi-dry transfer system (Bio-Rad Catalog #1704150 ...
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bioRxiv - Cell Biology 2019Quote: ... Five μg of proteins were loaded onto a 4-12% (wt/vol) NuPAGE Bis-Tris gel (Thermo Fisher Scientific), and SDS-PAGE and immunoblotting were conducted following standard protocols ...
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bioRxiv - Genomics 2020Quote: ... The samples were then loaded onto a NuPAGE 4-12% Bis-Tris Protein Gel (Thermo Fisher Scientific Cat# NP0335BOX) and a short gel was run using NuPAGE MOPS SDS Running Buffer (Thermo Fisher Scientific Cat# NP0001 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 20 μg of soluble protein per sample were run in a 4-20% Tris-Glycine gradient polyacrylamide gel (Invitrogen) and transferred to a ProTran NitroCellulose transfer membrane (Whatman ...
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bioRxiv - Cell Biology 2019Quote: ... CHMP4B was labelled overnight at 4°C with Alexa 555 succimidyl ester or 633 succimidyl ester (Thermo Fisher Scientific) using a molar ratio (Alexa Fluor:protein ...
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bioRxiv - Genetics 2020Quote: 1.5 × 105 CD34+ cells were deposited on glass slides by centrifugation using a Cytospin(tm) 4 cytocentrifuge (Thermo Scientific) for 5 min at 800 rpm ...
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bioRxiv - Bioengineering 2019Quote: HESC-CMs were replated into wells of a 4-well NuncTM chamber slide at 60k cells/well (Thermo Scientific) following immobilization of either IgG or Delta-1 and supplemented with 5 µg/ml fibronectin as described above ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA fragments were sheared into 300bp fragments using Bioruptor sonication at 4°C (Ion Torrent, Life Technologies, NY, USA). Following preclearing with salmon sperm/Protein A agarose slurry (GE Healthcare ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tube was then centrifuged at 1600 rpm for 10 minutes at 4°C and the pellet resuspended in Hank’s Buffered Saline Solution (#14175095, Gibco, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... and left to stratify in the darkness at 4 C for two days on ½ MS plates (0.43% (w/v) MS Salts (Gibco), 1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated using SDS–PAGE with Novex 4–20% Tris-glycine polyacrylamide gel (Invitrogen, Life Technologies, Carlsbad, CA). Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated using SDS–PAGE with Novex 4–20% Tris-glycine polyacrylamide gel (Invitrogen, Life Technologies, Carlsbad, CA). Western blot analysis was performed using peroxide-conjugated anti-hemaglutinin antibody at 1:250 dilution (Roche) ...
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bioRxiv - Developmental Biology 2019Quote: Primary chondrocytes were isolated from limb buds of E12.5 embryos by digestion with 1 U/ml Neutral Protease NB (Serva) for 15 min and with 0.3 U/ml collagenase NB 4 (Serva)/0.05 % trypsin-EDTA (Life Technologies) for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Enzyme activity was stopped by addition of equal volume of 4% BSA in Earle’s Balanced Salt Solution (EBSS, Gibco). The cell suspension was filtered through a 70 μm cell strainer and centrifuged at 1200 rpm for 5 min and the pellet was re-suspended in 0.9 M sucrose in 0.5 x HBSS (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: ... boiled for 5 min at 90°C and loaded on NuPAGE Novex 4–12% Bis-Tris Protein Gels (Invitrogen). Gels were then fixed for 30 min at RT in fixing solution (40% [v/v] methanol ...
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bioRxiv - Biochemistry 2019Quote: ... Imidazole was removed by desalting the protein solution at 4°C using Zeba spin 7K desalting column (ThermoFisher Scientific) into 50 mM potassium phosphate buffer pH 7.3 ...
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bioRxiv - Physiology 2021Quote: ... for 60 minutes at 4 °C and the reaction was stopped with 10 μl SuperaseIn (Thermo Fisher Scientific AM2696). Ribosome-protected fragments were isolated using microspin S-400 (GE HealthCare ...
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bioRxiv - Cancer Biology 2021Quote: ... 25 μg of protein per lane were resolved on NuPAGE Novex Bis-Tris 4-12% polyacrylamide gels (Invitrogen, #NP0321BOX) and transferred onto nitrocellulose blotting membranes (Amersham Protran ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies). ImageJ/Fiji software (National Institutes of Health ...
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bioRxiv - Immunology 2021Quote: ... proteins with a molecular weight (MW) below 100 kDa were separated on 4-12% Bis/Tris gels (Life Technologies) in MES SDS Buffer (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun to remove any debris and loaded onto a 4-12% Bis-Tris NuPAGE gel (Thermo Fisher) and separated for 3 hours at 4C at 100V ...
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bioRxiv - Microbiology 2021Quote: ... Harvested cells were normalized to the lowest OD-600 of the collected cells and pelleted by centrifugation at 20000XG for 2 mins and resuspended in 75 µL lysis buffer (10 mM Tris-HCl, pH 7.0, 4% SDS, 5 U DNaseI (ThermoFisher), 0.2 tablets of PhosStop (Roche)) ...
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bioRxiv - Systems Biology 2020Quote: ... DNA was checked for quality by gel electrophorese and by using a Qubit 4 Fluorometer (Thermo Fisher Scientific, USA). Metagenomic sequencing was performed by Novogene Ltd ...
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bioRxiv - Neuroscience 2021Quote: ... extracted nuclear proteins were subjected to NuPAGE Bis-Tris (4 to 12%) gel electrophoresis under reducing conditions (ThermoFisher Scientific) and then electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... cells were grown in Eagle’s Minimum Essential Medium (EMEM) (Cellgro) supplemented with 4% heat inactivated fetal bovine serum (FBS) (Gibco) at 37° C and 5% CO2.
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were mounted in ProLong Gold antifade reagent supplemented with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes). Conventional immunocytochemical staining was done to quantify the activated PAK1 and actin in oAβ and IPA-3 treated cells using phospho-PAK1 (Thr423)/PAK2 (Thr402 ...
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bioRxiv - Systems Biology 2021Quote: ... Fat and lower dermis was cut away and discarded before dispase (2 U/ml, Gibco, UK, 20h, +4°C) digestion ...
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bioRxiv - Genetics 2021Quote: ... The libraries were subjected to size selection (250–600 bp) on a 4–20% TBE PAGE gel (Thermo Scientific). The TBE gel was run at 180V ...
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bioRxiv - Biochemistry 2020Quote: ... were then separated by 4 to 12% bis-tris SDS-PAGE in NuPAGE MOPS SDS Running Buffer (Life Technologies) either at room temperature (for Western blot ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated on NuPAGE™ Novex® 4-12% Bis-Tris protein gels in MES buffer (Life Technologies) and were transferred onto nitrocellulose membranes using the iBlot® system (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... Yields were measured using the Qubit dsDNA HS Assay Kit with a Qubit 4 fluorometer according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Bioengineering 2021Quote: ... Bacteria were washed and pelleted with PBS via centrifugation (13,180 × g at 4 °C for 10 min) with a superspeed centrifuge (ThermoFisher). Afterwards ...
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bioRxiv - Bioengineering 2020Quote: ... The tissues were incubated in the recommended concentrations of Fluo-4 direct according to the manufacturer (Molecular Probes, F10471) for 50 min in the incubator at 37 °C and 10 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were stored at 4°C in an autosampler and analyzed using a Vanquish LC system (Thermo Fisher Scientific), coupled to a 6500+ QTrap mass spectrometer (SCIEX ...
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bioRxiv - Molecular Biology 2020Quote: Full-length N was separated from the lower molecular weight proteoforms on a 0.8 mm 4-12% bis-tris SDS-PAGE gel (Invitrogen) and stained with Coomassie Blue ...