Labshake search
Citations for Thermo Fisher :
9351 - 9400 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... and stored at 4 °C overnight prior to storage at −80 °C following RNAlaterTM best practices (Life Technologies, 2011). Following this ...
-
bioRxiv - Biochemistry 2024Quote: ... cell suspensions were incubated for 20 min at 4°C in 80 µL of L15 medium containing 2.1 µg of anti-rat CD2 antibody (Invitrogen) per 15 million dissociated cells ...
-
bioRxiv - Molecular Biology 2024Quote: ... and two sets of triplicate technical replicates were run on NuPage 4-12% Bis-Tris SDS Page (ThermoFisher Scientific) gels in 10 mM DTT/1x loading buffer ...
-
bioRxiv - Microbiology 2024Quote: ... and 2 µL RNase A/T1 Mix (4 µg RNase A, 10 U RNase T1, ThermoFisher Scientific, MA, USA) at 37°C for 30 min to remove host-originating nucleic acids ...
-
bioRxiv - Microbiology 2024Quote: Primary CF-HBECs from 4 donors were polarized on 24 mm Transwell filters and washed with DPBS (ThermoFisher Scientific) before treatment ...
-
bioRxiv - Microbiology 2024Quote: ... then cooled and measured for total protein by Qubit 4 using the Protein Assay Quantification Kit (Life Technologies, Q33211), according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: ... each heat inactivated sample was 4-fold serially diluted in Eagle’s Minimum Essential Medium (EMEM) (Cellgro) supplemented with 4% heat inactivated fetal bovine serum (FBS) (Gibco) and mixed with an equal volume of SARS-CoV-2 isolate USA-WA1/2020 from the Biological and Emerging Infections Resources Program (BEI Resources ...
-
bioRxiv - Molecular Biology 2024Quote: ... before being incubated for 20 min at 4°C with anti-V5-FITC or anti-V5-PE (ThermoFisher Scientific) and anti-CD8-APC (Miltenyi Biotec) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were heated to 80°C for 5 min and electrophoresed on 4-12% NuPAGE Bis-Tris gels (ThermoFisher). Wet transfer was to a nitrocellulose membrane (0.45 uM pore ...
-
bioRxiv - Molecular Biology 2024Quote: ... 4 days post doxycycline induction cells were incubated in a cell culture medium with Hoechst 33342 (Thermo Fisher Scientific) at a final concentration 5 μg/ml for 30 min at 37°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 µL of each sample was loaded into a well of NativePAGE 4-12% Bis-Tris Gels (Invitrogen NP0321BOX). The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010 ...
-
bioRxiv - Molecular Biology 2024Quote: Full-length 6E11 antibody was digested over 4 hours at 37 °C using immobilized ficin agarose resin (Thermo Fisher) and then clarified by size-exclusion chromatography using a Superdex Increase 200 column (Cytiva ...
-
bioRxiv - Molecular Biology 2024Quote: Equivalent volumes of each fraction were loaded onto a 4-12 % Bis-Tris NuPAGE SDS-PAGE gel (Life Technologies) and separated by molecular weight using denaturing electrophoresis ...
-
bioRxiv - Microbiology 2024Quote: ... Purified proteins were examined using NuPAGE Bis-Tris 4-12% acrylamide gels with MES as the running buffer (Invitrogen). Proteins were stained AcquaStain Protein Gel Stain (Bulldog-Bio).
-
bioRxiv - Molecular Biology 2024Quote: ... Debris was pelleted at 15,000 RCF for 15 min at 4°C and protein concentration determined using a BCA assay (Invitrogen). Protein (50 μg per sample ...
-
bioRxiv - Developmental Biology 2024Quote: ... Between 20-30 μg of whole cell lysate were loaded in Novex WedgeWell 4–20% Tris-Glycine Gel (Invitrogen) and subject to SDS-PAGE ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein extracts (30 μg) were resolved by SDS-PAGE on Mini 4% - 20% Novex Tris-Glycine Gels (Thermo Fisher). Primary antibodies were incubated overnight at 4°C (Supplementary Table 4) ...
-
bioRxiv - Neuroscience 2024Quote: ... 10 μg of total protein per sample was loaded onto 4 to 12% bis-tris SDS- polyacrylamide gel (Invitrogen). Following electrophoresis ...
-
bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2024Quote: ... Gels were functionalized with 0.1M sulfo-succinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Cat# 22589, Thermo Scientific) dissolved in HEPES before overnight incubation in collagen type-I solution (Catalogue # A1048301 ...
-
bioRxiv - Microbiology 2024Quote: ... 30 μL of each concentrated sample was run on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Scientific, catalog number ...
-
bioRxiv - Cell Biology 2024Quote: ... Lysates (35-40 µg) were separated by SDS-PAGE on NuPage 4-12% gels (Bis-Tris Gel; Invitrogen, #NP0335BOX), transferred to polyvinylidene fluoride membranes and blocked in 5% (w/v ...
-
bioRxiv - Cell Biology 2024Quote: ... or C12E8 (0.5 mg/mg protein) and purified by a brief SDS-PAGE run (Nu-PAGE 4-12%, Invitrogen). Lanes were excised ...
-
bioRxiv - Cell Biology 2024Quote: ... and aliquots containing 5 × 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen). The sample lane was divided into eight slices that were excised from the Coomassie-stained gel ...
-
bioRxiv - Biochemistry 2023Quote: ... before being incubated for 20 min at 4°C with anti-V5-FITC or anti-V5-PE (ThermoFisher Scientific) and anti-CD8-APC (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 μg of samples were loaded on SDS-PAGE gel (Novex 4%–20% Tris-Glycine Mini Gels, Thermo Scientific). Protein was transferred to a PVDF membrane 0.2 μm (Amersham ...
-
bioRxiv - Plant Biology 2023Quote: ... The final PCR products (round 4) were subcloned into the pJET vector (CloneJET PCR Cloning Kit, Thermo Fisher Scientific) and sequenced ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR products were then cloned into the pCR™4-TOPO® TA cloning vector (Thermo Fisher Scientific) and the clones were confirmed by restriction digestion with BamHI ...
-
bioRxiv - Molecular Biology 2024Quote: ... and samples containing 30 µg total protein were analyzed by western blotting on 4-12% Bis-Tris Gels (Invitrogen). Proteins were transferred onto nitrocellulose membranes in accordance with the manufacturer’s instructions (Biorad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended in 500 µl of 4% BSA in HBSS and counted using Trypan Blue staining (Gibco) and a Countess cell counter (Invitrogen) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10–15 µL of protein lysis were run per lane of 4–12% Bis-Tris gels (Thermo Fisher Scientific) using MOPS buffer ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis in NuPage 4-12% Bis-tris gels run with 1X MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 µg protein sample was loaded in each well and ran on a 4%–12% SDS-PAGE gel (Invitrogen), then transferred to the nitrocellulose membrane (0.2 µm ...
-
bioRxiv - Cell Biology 2024Quote: Cells for immunofluorescence analysis of centrosomes were fixed using pre-chilled (−20°C) methanol (ThermoFisher Scientific Cat. # A412P-4) for 10 minutes at −20°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... zebrafish larvae were dechorionated manually and fixed at 28-30 hpf with 4% PFA (043368-9M, Thermo Fisher Scientific) in PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... while 10 μg/lane of lysates were electrophoresed on Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific) for β-actin ...
-
bioRxiv - Developmental Biology 2024Quote: ... Chromatin was released from crosslinked nuclei by dissolving 300 μl of SDS buffer (50 mM HEPES pH 7.4, 10 mM EDTA pH 8.0, 4% UltraPure™ SDS Solution from Invitrogen, along with a newly added 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
-
bioRxiv - Microbiology 2024Quote: ... the bound proteins resolved using 4-12% Novex Tris-glycine gels and silver-stained (MS compatible) (Thermo Fisher Scientific). Trypsin digestions of the 0.2% FA elution were processed for mass spectrometric (MS ...
-
bioRxiv - Microbiology 2024Quote: ... for 24 hours at 4 °C and subsequently incubated with streptavidin Alexa-488 (Invitrogen Molecular Probes, Carlsbad, CA, USA) in a 1:500 dilution for 1 hour at room temperature.
-
bioRxiv - Microbiology 2024Quote: ... for 24 hours at 4 °C and subsequently incubated with streptavidin Alexa-488 (Invitrogen Molecular Probes, Carlsbad, CA, USA) in a 1:500 dilution for 1 hour at room temperature.
-
bioRxiv - Immunology 2024Quote: ... the antibody was reduced by incubating it with a final concentration of 4 µM TCEP (Thermo Fisher Scientific, USA) for 30 mins in a water bath maintained at 37 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
-
bioRxiv - Biochemistry 2024Quote: ... A fresh 4% formaldehyde solution was prepared in PBS by diluting from a 16% methanol-free stock (Thermo Scientific). Cells were treated with formaldehyde for 10 minutes at 25°C under gentle mixing then washed with PBS ...
-
bioRxiv - Biochemistry 2024Quote: HeLa cells (Leibnitz Institute DSMZ; ACC 57) at passage 4-25 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Ref ...
-
bioRxiv - Biochemistry 2024Quote: Reduced protein extracts were separated on 10% SDS–PAGE gels or 4–12% NuPAGE bis–tris precast gels (Invitrogen) by electrophoresis ...
-
bioRxiv - Cancer Biology 2024Quote: ... Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher) at 37 °C ...
-
bioRxiv - Cancer Biology 2024Quote: ... The gels were transferred at 100 V for 1.5 hours at 4°C in transfer buffer (ThermoFisher Scientific, NP0006) onto PVDF membranes (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein per sample were separated on 4 to 20% Tris-glycine gradient gels (XP04200BOX, Thermofisher Scientific) and transferred to nitrocellulose membranes ...
-
bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and nuclei were stained with 4’-6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and mounted with an aqueous mounting medium (Aqua-Poly/Mount ...
-
bioRxiv - Cell Biology 2024Quote: ... The 2D well of the pre-cast NuPAGE 4%-12% Bis-tris 2D well gel (Thermo Fisher Scientific, NP0326BOX) was washed and filled with 1x MOPS running buffer (Thermo Fisher Scientific ...