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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were maintained in embryo media at 28.5°C until 5 DPF and fixed in 4% paraformaldehyde (Fisher Scientific) at room temperature for 1 hour and stored in 1x PBS (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cell lysates were incubated with RNA-aptamer-coupled beads for 3.5 h at 4°C under rotation in the presence of 60U RNasin (Ambion). For each pull-down ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected and stained for neutral lipids by BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a, 4a-Diaza-s-Indacene; ThermoFisher Scientific; cat# D3922) or HCS LipidTOX™ Red Neutral Lipid Stain (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Comparable amounts of proteins were subjected to SDS-PAGE using NuPAGE 4% to 12% Bis–Tris gradient gels (Invitrogen) and were transferred to PVDF membranes ...
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Remodeling of the Ribosomal Quality Control and Protein Translation by a Viral Ubiquitin DeconjugasebioRxiv - Cell Biology 2023Quote: ... Equal amounts of lysates were fractionated in acrylamide Bis-Tris 4-12% gradient gel (Life Technologies Corporation, Carlsbad, USA). After transfer to PVDF membranes (Millipore Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 µg of each sample was loaded on a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific, USA) and run at 180 V for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Fixation was performed with 4% paraformaldehyde or Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific; 00-5523-00) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed twice with PBS before fixation with PBS containing 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... in phosphate buffered saline (PBS) for 15 min at room temperature or in 100% methanol (A412-4, Fisher Scientific) for 5 min at -20°C ...
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bioRxiv - Cell Biology 2023Quote: ... in phosphate buffered saline (PBS) for 15 min at room temperature or in 100% methanol (A412-4, Fisher Scientific) for 5 min at -20°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated in an NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (WG1402BOX, Thermo Fisher Scientific) in NuPAGE™ MOPS SDS running buffer (50 mM MOPS ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were prepared with 30 μg of protein in RIPA buffer with 4× LDS loading buffer (LP0001, Life Technologies). Samples were electrophoresed on SDS/PAGE gels ...
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bioRxiv - Genetics 2023Quote: ... When 80% confluent (approximately every 4 days) hiPSCs were dissociated using 0.5 mM EDTA (Invitrogen; cat. no. 15575-038) in DPBS (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... human stool extracts and mouse colonic tissue lysates were resolved on Bolt 4-12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated by SDS-PAGE using a 4-12% NuPAGE Bis-Tris gel (Thermo Fisher Scientific Cat# NP0321BOX) in NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific Cat# NP0001) ...
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bioRxiv - Developmental Biology 2023Quote: ... Images were collected manually or automatically with 4-6 µs dwell time using Maps 3.11 software (Thermo Fisher Scientific) (Burel et al. ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was collected from EO771.LMB tumors that were xenografted into B6 and PWD.B6 mice (n = 4 per group) isolated using TRIzol Reagent (ThermoFisher), poly-A purified ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Plant Biology 2023Quote: ... 23°C) and separated on linear gradient 4-12% Bis-Tris polyacrylamide gels (NuPAGE® Novex®, Invitrogen, USA) in MES running buffer (50 mM MES ...
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bioRxiv - Biochemistry 2023Quote: ... the DNA was quantified using the Qubit 1X dsDNA HS assay kit and a Qubit 4 Fluorometer (both Invitrogen), and the DNA of the insertion libraries from the same assay was pooled in an equimolar ratio ...
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bioRxiv - Developmental Biology 2023Quote: ... the cell pellet was gently washed with 4% BCS/PBS and resuspended in 100 µl of 0.1% Ultrapure Bovine Serum Albumin (BSA)/PBS (Invitrogen). Cell number and viability was assessed using trypan exclusion and a TC20 automated cell counter (BioRad) ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Quantity and quality of the dsRNA was assessed by fluorometric analysis using a Qubit 4 Fluorometer (Thermo Fisher Scientific). dsRNA was stored in the dark at −80°C until usage.
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bioRxiv - Microbiology 2023Quote: ... 100 ng of pCI-VSV-L were mixed with 4 ml of Plus reagent and 6.6 ml of Lipofectamine LTX (Invitrogen). 30 minutes later ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μg of protein from extracts were separated in NuPAGE 4-12% Bis-Tris Gel 1.0mm (NP0321BOX, Thermo Scientific) or 3-8% Tri-Acetate gel (EA03755BOX ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant (30 μl) was loaded on a NuPAGE Bis-Tris 4-12% polyacrylamide gel (Invitrogen, Massachusetts, United States), and run in MOPS buffer at 200 V ...
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bioRxiv - Biochemistry 2023Quote: NMA1982 phosphatase activity was measured using a standard fluorescence intensity phosphatase assay utilizing 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP; Invitrogen) as the substrate [26] ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were mounted with Fluoromount G™ containing 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI, Invitrogen) for nuclei detection ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with PBS and set in a maintenance medium that contained 4% BSA Fraction V (Gibco) and 0.1% L-(tosylamindo-2-phenyl ...
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bioRxiv - Genetics 2023Quote: Proteins were separated by SDS-PAGE electrophoresis on a Bolt 4-12% Bis-Tris Plus Gel (Thermo Fisher Scientific) and then transferred onto a 0.45-µm nitrocellulose membrane in a Mini Trans-Blot Cell (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... 20Lμg of cell protein extract per sample was separated on 4-12% Bolt® Bis-Tris Plus gels (ThermoFisher) using MOPS SDS NuPAGE Running Buffer (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... cell suspension from isolated females infecting tomato roots was applied on slides using Cytospin 4 cytocentrifuge (Shandon, ThermoFisher Scientific). Slides were incubated for 20 min at -20 °C in methanol:acetone (1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were stored in 2% PFA at 4°C prior to analysis using an Attune NxT Flow Cytometer (Invitrogen) in the Flow Cytometry Core Facility of the Faculty of Medicine & Dentistry at the University of Alberta ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...
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bioRxiv - Genomics 2023Quote: CD56pos cells were seeded at 3,000 cells per cm2 in 4 well Permanox Nunc Lab-Tek chambers (ThermoFisher #177437) and cultured in growth medium ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were centrifuged at 14,000 x g at 4°C for 20 minutes and quantified with Pierce BCA Protein Assay Kit (ThermoFisher). 10 μg or 20 μg of proteins were separated by electrophoresis in denaturing SDS polyacrylamide gel (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... 15 μg of each sample was loaded onto a 4-12% Bis-Tris SDS-PAGE gel (Thermo Fisher, WG1401A). Two μL of MagicMark XP Western Protein Standard (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 50μg of lysate was mixed with SDS loading buffer and loaded into 4%-12% precast gels (NP0335, Life Technologies). For BRCA1 western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins in cell lysates (20-40 μg) were separated on precast NuPAGE™ 4-12% Bis-Tris gels (Invitrogen) using MES (Figure 4 ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µg packaging plasmid (psPAX2) and 2.5 µg envelope plasmid (pMD2.G) using Lipofectamine 2000 in OptiMEM (ThermoFisher Scientific). After 6-hr incubation of Lipofectamine/DNA mixture in OptiMEM ...
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bioRxiv - Developmental Biology 2023Quote: ... resuspended in mounting media containing 4’,6’-diamidino-2-phenylindole (DAPI) (Fluoromount-G™ Mounting Medium, with DAPI, Invitrogen), incubated overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Total cell lysates were prepared and analyzed by SDS-PAGE using 4-12% gradient Bis-Tris NuPAGE gels (ThermoFisher) followed by immunoblotting71 ...
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bioRxiv - Cancer Biology 2023Quote: ... and A260/A230 and A260/280 ratios of RNA were determined by the Qubit 4 fluorometer (Thermo Fisher Scientific), and the DeNovix Spectrophotometer DS 11 (DeNovix Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg of protein were resolved using a NuPAGE (4– 12%) Bis-Tris gel (Thermo-Fisher Scientific, Cat. # NP0322PK2). After the run ...
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bioRxiv - Immunology 2022Quote: ... filtered through a 70μm cell strainer and centrifuged at 4°C for 10min at 400xg before resuspending cells in D-PBS (Gibco) determining cell counts and viability using a nucleocounter® ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... Equal amounts of protein were loaded and resolved on a 4–12% Bis-Tris gradient gel (Thermo Fisher Scientific) and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of protein samples within each group were loaded and resolved using 4–12% NuPAGE gels (Life Technologies), and subsequently electro-transferred onto nitrocellulose membrane (iBlot ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were prepared and run on NativePage 4-12% Bis-Tris gels as per the manufacturer’s instructions (Life Technologies). For immunoblotting ...
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bioRxiv - Microbiology 2023Quote: ... (BEI NR-52310) or pSARS2-SΔCT and 4 μg pTRMPSS2 (VRC/NIAID) diluted in 2 mL OPTIMEM media (Gibco) (DNA OPTIMEM mixture) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial membranes were stained in this suspension with 50 µl of 100 mg/mL FM 4-64FX (Molecular Probes) and washed twice in PBS by centrifugation for 7,000 x g at RT ...