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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Explants were suspended in 90% foetal bovine serum (Gibco Invitrogen Saint Aubin, France)/ 10% dimethyl sulfoxide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Explants were suspended in 90% foetal bovine serum (Gibco Invitrogen Saint Aubin, France)/ 10% dimethyl sulfoxide (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and 90% heat-inactivated fetal bovine serum (FBS; GIBCO, California, USA; cat. 11560636), and stored at −80 °C until use.
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with streptavidin-Alexa 488 (1:750, 90 min, Molecular Probes). Next ...
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bioRxiv - Bioengineering 2023Quote: ... or resuspended in freezing media (90% fetal bovine serum (FBS, Life Technologies, 10438018) + 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2023Quote: ... labeled with eFluor670 according to manufacturer’s protocol (Thermo Fisher, Cat#: 64-0840-90), then exposed to 217.8 mJ/cm2 UV irradiation for 3 min to induce apoptosis ...
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bioRxiv - Cell Biology 2022Quote: ... RNAs were isolated from the rest 90% of precipitates with TRIzol LS (Invitrogen) according to the standard manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded at a density of 80-90 % into chamber slides (Nunc) and fixed after 24 h with 4% paraformaldehyde at room temperature for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... NPSCs were passaged upon reaching 85-90% confluency using Accutase (Thermo fisher, A1110501). If applicable ...
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bioRxiv - Genetics 2023Quote: ... HEK293T cells were cultured to 90-95% confluency in DMEM (Life Technologies 11995081) supplemented with 10% FBS (Sigma-Aldrich F2442 ...
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bioRxiv - Genomics 2023Quote: ... primary antibodies (90-100 μg) were buffer-exchanged to 1× PBS (Invitrogen AM9624) using 50KDa Amicon Ultra Centrifugal Filter Unit (Millipore ...
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bioRxiv - Pathology 2024Quote: ... and run with SDS running buffer for 90 minutes (LC2675, Thermo Fisher Scientific). Gels were renatured in Zymogram Renaturing Buffer (LC2670 ...
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bioRxiv - Molecular Biology 2024Quote: Ba/F3 cells were purchased from DSMZ and maintained in 90% RPMI (Gibco), 10% HI-FBS (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... HEK 293T cells were transfected at 75-90% confluency with Lipofectamine 2000 (Invitrogen) in 35mm 1.5 coverglass bottom dishes MatTek dishes ...
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bioRxiv - Molecular Biology 2024Quote: ... and IMR-90 tert (human lung fibroblast) cells were cultured in DMEM (Gibco), supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were grown to near confluence (90-95%) in either Stemflex (Thermofisher #A3349401) or in mTeSR Plus (StemCell Technologies #100-0276 ...
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bioRxiv - Cell Biology 2024Quote: ... and >90% humidity and were passaged at 80% confluency with Trypsin (#25300054, Thermofisher). For all the experiments ...
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bioRxiv - Biochemistry 2024Quote: ... ∼90% confluent cells were either treated 4 h with HBSS media (ThermoFisher, 24020117) with or without 50 μM Bafilomycin (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected at 80-90% confluency with Lipofectamine 2000 reagent (Invitrogen, UK).
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bioRxiv - Biochemistry 2020Quote: Beads were first activated with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (Thermo Fisher Scientific) in the presence of N-hydroxysuccinimide (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Analytical grade cefepime hydrochloride (Apotex Corporation, Weston, FL, USA) and ceftazidime (Acros Organics, NJ, USA) pentahydrate as an internal standard were used for liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Microbiology 2022Quote: ... or L(+)-arginine hydrochloride (126.5 μg/ml; Acros Organics, New Jersey; per Davis RW, 1990) were added to the DvH cultures ...
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bioRxiv - Physiology 2023Quote: ... USA) with exception of the pyridine and methoxyamine hydrochloride (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Biophysics 2024Quote: ... and 250 µM of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Thermo Scientific, 24510) in Borate buffer (100 mM Borate-NaOH ...
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bioRxiv - Plant Biology 2020Quote: ... cinerea germlings exposed to different concentrations NCR044.1 was measured every 30 min for 2 h using the ROS indicator dye 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA, Invitrogen, Carlsbad, CA). ROS levels were quantified by measuring the fluorescence in a SpectraMax® M3 spectrophotometer microplate reader (exc ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... The gel was imaged and washed 2 x 15 minutes in nuclease-free water then 2 x 15 minutes in 10X UltraPure SSC Buffer (Invitrogen, Carlsbad, CA) with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Genomics 2022Quote: ... Individual DGs were removed from the sting complex using clean forceps and pooled according to treatment group in sterilized 2 mL FastPrep tubes containing six 2 mm zirconia beads and 250 µL Trizol (Invitrogen, Carlsbad, CA). Individual DGs were pooled to obtain sufficient amounts of RNA for downstream analyses ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Cell Biology 2020Quote: ... Treated or untreated parasites were incubated with 100 mM LysoTracker™ Red DND-99 (ThermoFisher Scientific Cat# L7528) in culture medium for 30 min at 26°C and analysed with a CytoFLEX flow cytometer (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... fresh BAL cell cytospin slides were stained using a Kwik-Dif Stains kit (Fisher Scientific, #99-907-00) for inflammatory cell differential counts ...
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bioRxiv - Neuroscience 2020Quote: ... cells were double-stained with live cell dyes Lysotracker Red DND-99 (Molecular Probes Cat. No. L-7528) and Mitotracker Deep Red FM (Molecular Probes Cat ...
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bioRxiv - Cell Biology 2022Quote: ... then searched with a database consisting of the top 20 Byonic-identified proteins (sorted by Log Probability and accounting for >99% of protein signal) using the MassAnalyzer algorithm embedded in BioPharma Finder software (version 4.1.53.14, Thermo Fisher) [43 ...
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bioRxiv - Microbiology 2021Quote: RNA-FISH was performed using the RNA View Cell Plus assay kit (Cat. No.88-19000-99, ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Growth medium was replaced with MEM supplemented with 0.05 μM LysoTracker® Red DND-99 (L7528, Life Technologies) and incubated for 30 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... >99% of BAL cells were found to be negative for fixable viability dye eFlour 780 (eBioscience, ThermoFisher Scientific), and hence ...
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bioRxiv - Biochemistry 2024Quote: ... were quantified by masking the signal for the lysosomal marker LAMP1 or LysoTracker Red DND-99 (Invitrogen #L7528).
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bioRxiv - Microbiology 2024Quote: ... THP-1 macrophages were incubated 30 min before the end of infection with Lysotracker red DND-99 (Invitrogen) at a final concentration of 75 nM ...
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bioRxiv - Neuroscience 2023Quote: ... cells were double-stained with live cell dyes Lysotracker Red DND-99 (Molecular Probes Cat. No. L-7528) and Mitotracker Deep Red FM (Molecular Probes Cat ...
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bioRxiv - Cell Biology 2023Quote: ... LysoTracker Red DND-99 (L7528) and Wheat Germ Agglutinin (WGA)-Alexa Fluor 594 (W11262) were obtained from ThermoFisher Scientific (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting reaction mixtures then were supplemented with 1 μl 0 or 1 mM ATP (>99% purity; ThermoFisher) in transcription buffer and incubated 5 min at 37°C (to enable termination) ...
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bioRxiv - Genetics 2024Quote: ... selected for genotype and transferred to Schneider’s insect medium containing 50nM Lysotracker™ Red DND-99 (Thermo Fisher) or DHE (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Legs/wings and bodies were placed into 2-mL tubes (Thermo Fisher Scientific, Emeryville, CA) containing 500 μL PBS and a 5-mm glass bead (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... mixed with DMEM supplemented with 2% FBS and penicillin/streptomycin (Thermo Fisher Scientific, Emeryville, CA) was added to each well to generate a solid agar plug ...
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bioRxiv - Bioengineering 2020Quote: ... then incubated in 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were stained with 4′-6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, CA, USA) before examination under a fluorescence microscope (Zeiss LSM 710).
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 4′,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Plant Biology 2023Quote: ... The Gateway recombination frame (Fragment 2, F2) was amplified from pEarleyGate 103 (Invitrogen, CA, USA) using the primer pair c/d (Table 1) ...
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bioRxiv - Developmental Biology 2022Quote: ... the tissue sections were stained with 4,6-diamidino-2-phenylindole (DAPI; Invitrogen, Carlsbad, CA, USA) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 12.5 μl 2 × SYBR™ Green PCR Master Mix (Life Technologies, Carlsbad, CA, United States), and 8.5 μl nuclease water ...