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Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... African green monkey kidney epithelial Vero-E6 cells (ATCC, CRL-1586) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2020Quote: Spinner-adapted L cells were grown in suspension culture in Joklik’s minimum essential medium (US Biological) supplemented to contain 5% fetal bovine serum (FBS) (Gibco), 2 mM L-glutamine (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Human cervical epithelial adenocarcinoma HeLa cells were cultured at 37° C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Blocks were sectioned to 5 μm thickness using a Leica microtome (RM2145) and applied to Ultrastick slides (Thermo Scientific). Sections were stored at room temperature until CLASI-FISH was performed.
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bioRxiv - Immunology 2021Quote: B16-F10 cells (ATCC, CRL-6475) were purchased and cultured at 37°C in 5% CO2 in DMEM (Invitrogen), 10% FCS (Benchmark) ...
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bioRxiv - Immunology 2020Quote: mRNA from 2-5 x 104 sorted cells was captured with 12 μl of Dynabeads oligo(dT) (Life Technologies), washed ...
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bioRxiv - Immunology 2021Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50µg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: HeLa and MelJuSo cell lines were maintained at 37°C/5% CO2 in Gibco Iscove’s Modified Dulbecco’s Medium (IMDM, Life Technologies-Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... RNA samples were first incubated at 65°C for 5 min in the presence of 0.5 mM dNTPs and 2.5 µM oligo(dT)20 (Life Technologies-Invitrogen). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... which were then transfected into Expi293F cells growing at 37 in 5% CO2 atmosphere using ExpiFectamine reagent (Thermofisher Scientific). Proteins were purified by strep-tag affinity and subsequently size exclusion chromatography.
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bioRxiv - Microbiology 2021Quote: ... Exponential phase starter cultures (OD600∼ 0.5 - 1.5) were diluted to OD600 = 0.01 and grown in 96-well Nunclon Delta surface clear plates (Thermo Scientific) with 150 μL per well ...
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bioRxiv - Immunology 2021Quote: ... replicate-wells of human IgG (40, 20, 10, 5, and 2.5 ng/µl in PBS with 10% glycerol) and AlexaFlour-555-Hydraside (Invitrogen, at 1 ng/µl in 178 mM phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... Slices were maintained in a humidified incubator with 5% CO2 at 37°C with 1 ml MEM (Gibco, 31095209) containing 20% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Equal concentrations of lysate (5 µg) were analyzed by reducing SDS-PAGE and transferred to a nitrocellulose membrane (Invitrogen). Membranes were washed with tris buffered saline (pH 7.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Then, 5 x106 cells were washed in PBS (HyClone Laboratories, Logan, UT, USA) containing 1 % BSA (Thermo Fisher Scientific) and stained in 100 μL PBS and BSA 1 %-containing Antibody cocktail ...
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bioRxiv - Immunology 2020Quote: Chloroquine diphosphate salt (CQ) (98%) and adenosine 5’-monophosphate sodium salt hydrate (AMP) (99%) were purchased from ACROS Organics™ ...
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bioRxiv - Immunology 2020Quote: ... Plates were blocked for 1 hr at room temperature with 200 μl 5% Blocker™ BLOTTO (Thermo Scientific, #37530) and washed three times with 200 μl PBS-T ...
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bioRxiv - Immunology 2021Quote: ... were diluted in two-fold dilution series from 1:5 to 1:5120 in Eagle’s Minimum Essential Medium (Gibco) with 5% FCS (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... lysed in 1X Novex buffer and 5-7.5×105 cells equivalents were resolved on 4-12% NuPAGE gel (Invitrogen) in MOPS buffer ...
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bioRxiv - Immunology 2021Quote: ... Remaining cells were centrifuged for 5 minutes at 500 g before 100µL of extraction buffer added (Arcturus Picopure RNA kit, Thermofisher) and lysates frozen at −80°C.
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bioRxiv - Microbiology 2021Quote: ... cell medium was replaced by fresh medium supplemented with 5 µM Hoechst 33342 nucleic acid stain (Thermo Fisher Scientific) as a background stain for high-content imaging analysis ...
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bioRxiv - Microbiology 2021Quote: ... 5 uL of re-suspended BAC16 DNA was incubated with 100 uL Opti-MEM reduced serum media (Thermo Fisher) for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and EFKB3 cells were cultured in Dulbecco’s minimal essential medium (DMEM) supplemented with heat inactivated fetal bovine serum(5 or 10%) and antibiotic/antimyotic (GIBCO). Both NP an OP swabs were used for virus isolation ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml of culture were grown in 20 mm × 150 mm glass test tubes (Fisher Scientific, Hampton, NH, USA) with disposable ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspendend in 1 mL M9 before 5 µL of DiI stock solution (2 mg/mL DiI (Molecular Probes, #D282) in dimethyl formamide ...
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bioRxiv - Cell Biology 2021Quote: ... a 6 cm dish of E14 cells 50% confluent was transfected using 2μg of pX459-Cas9-sgRNA and 5 μg of targeting construct using Lipofectamine 2000 (ThermoFisher) according to manufacturer’s guidelines ...
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bioRxiv - Genetics 2019Quote: ... 5% CO2) to 70-80% confluence in dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Treated sections were then mounted with hybridization buffer (5%/15% Formamide; 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616 ...
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bioRxiv - Genetics 2019Quote: ... 10% β-mercaptoethanol at 95°C for 5 min and run on NuPage 10% Bis-Tris gels (Life Technologies) in 2-(N-morpholino ...
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bioRxiv - Genomics 2021Quote: ... 0.5μM oligo-dT (IDT; 100uM 5′-biotin-ACGAGCATCAGCAGCATACGA-T30VN-3′) and 0.5mM dNTPs/each (Thermo Fisher; 25 mM each) and snap frozen at −80 °C ...
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bioRxiv - Genomics 2021Quote: ... The presence of SARS-CoV-2 was determined using the 2019-nCoV CDC assay (https://www.fda.gov/media/134922/download) on the QuantStudio 5 (Applied Biosystems) (n=7) ...
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bioRxiv - Genomics 2019Quote: ... Cells were cultured at 37°C with 5% CO2 in Roswell Park Memorial Institute (RPMI) medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2019Quote: ... Cells were cultured at 37°C with 5% CO2 in Ham’s F-12 Nutrient Mixture medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: ... urartu was labelled by nick translation with ChromaTide™ Alexa Fluor™ 488-5-dUTP (Invitrogen; C11397; coloured green), 2 ...
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bioRxiv - Genetics 2020Quote: ... After 5 min incubation with the developing reagent was added (Pierce® ECL Western Blotting Substrate, 32106, Thermo Scientific) and images were captured using Azure 300 imaging system (Azure Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% ACN/0.1% formic acid at a flow of 250 nL/min for 90 min and analyzed with a QExactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The full structure of the NUKU2 transcript was determined with 5’ and 3’ RACE using the GeneRacer kit (Invitrogen), and testicle total RNA (Ambion ...
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bioRxiv - Microbiology 2020Quote: Spinner-adapted L929 cells were grown in suspension culture in Joklik’s minimum essential medium (JMEM; US Biological) supplemented to contain 5% fetal bovine serum (FBS) (Gibco), and 2 mM L-glutamine (Corning) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were seeded at densities of 3 × 104 or 5 × 104 per 60 mm plate with a bottom layer of 0.6% Noble agar in MEM (Gibco) and a top layer of 0.5% methylcellulose containing MEGM (Lonza) ...
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bioRxiv - Microbiology 2022Quote: MDCK cells were seeded in 96-well microplates at a density of 5×103 cells/well in 100 µl Gibco Dulbecco’s Modified Eagle Medium (DMEM) (Gibco® ...
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bioRxiv - Developmental Biology 2022Quote: ... complexes were formed by mixing the sgRNA (2.5 ng/uL) with Cas9 protein (IDT, 5 ng/uL) in Opti-MEM (ThermoFisher) and incubating at 37C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were cultured at 37°C with 5% CO2 for 3 days in RPMI-1640 medium (Thermo Fisher Scientific) supplemented 10% FCS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... SK-MEL-28 and SK-MEL-5 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies Corporation) with 10% fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in a humidified incubator at 37°C under 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5 µg of pCS2P+dkk1GFP plasmid in using Lipofectamine 2000 (Thermo Fisher Scientific, MA, USA). Dkk1GFP CM were collected on the 2nd ...
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bioRxiv - Developmental Biology 2022Quote: ... In brief: Cells were maintained in a humidified incubator at 5% CO2 and 37 °C (Thermo Fisher Scientific, USA).